Streptococcus satellite phage Javan216

Taxonomy: Viruses; unclassified bacterial viruses

Average proteome isoelectric point is 6.24

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 12 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A4D5ZGM5|A0A4D5ZGM5_9VIRU Uncharacterized protein OS=Streptococcus satellite phage Javan216 OX=2558573 GN=JavanS216_0003 PE=4 SV=1
MM1 pKa = 7.55NEE3 pKa = 3.84QEE5 pKa = 4.8KK6 pKa = 11.09DD7 pKa = 3.55CIYY10 pKa = 11.61NMIDD14 pKa = 3.33LADD17 pKa = 3.77NAILQGDD24 pKa = 4.13KK25 pKa = 11.22DD26 pKa = 3.71HH27 pKa = 7.22ALTSLYY33 pKa = 10.25FIKK36 pKa = 10.56KK37 pKa = 9.09GLQALSMGMEE47 pKa = 4.4ANN49 pKa = 3.6

Molecular weight: 5.51 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A4D5ZH32|A0A4D5ZH32_9VIRU Paratox OS=Streptococcus satellite phage Javan216 OX=2558573 GN=JavanS216_0001 PE=4 SV=1
MM1 pKa = 8.09AEE3 pKa = 3.91IKK5 pKa = 10.55KK6 pKa = 8.29ITKK9 pKa = 10.26KK10 pKa = 10.74NGTTVYY16 pKa = 9.03RR17 pKa = 11.84TNIYY21 pKa = 10.48LGIDD25 pKa = 3.15KK26 pKa = 9.58ATGKK30 pKa = 8.28QARR33 pKa = 11.84TTITANTKK41 pKa = 9.99KK42 pKa = 10.4GVKK45 pKa = 9.38IKK47 pKa = 10.3IRR49 pKa = 11.84EE50 pKa = 4.5AINEE54 pKa = 4.1FARR57 pKa = 11.84NGYY60 pKa = 6.25TVKK63 pKa = 10.73KK64 pKa = 9.07KK65 pKa = 11.2VNVKK69 pKa = 8.45TYY71 pKa = 10.81RR72 pKa = 11.84EE73 pKa = 4.3LVSLWWDD80 pKa = 3.44SYY82 pKa = 11.76KK83 pKa = 10.2NTVKK87 pKa = 10.65PNTEE91 pKa = 4.03KK92 pKa = 10.25TVKK95 pKa = 10.36SYY97 pKa = 11.71LEE99 pKa = 4.46ANLLPAFGEE108 pKa = 4.1YY109 pKa = 10.48RR110 pKa = 11.84LDD112 pKa = 3.71NLTTPVIQKK121 pKa = 9.17QVNKK125 pKa = 8.94WANKK129 pKa = 9.9ANRR132 pKa = 11.84QSKK135 pKa = 9.37NAFGSYY141 pKa = 9.86HH142 pKa = 6.73KK143 pKa = 10.85LVGLNKK149 pKa = 10.22RR150 pKa = 11.84ILKK153 pKa = 10.44YY154 pKa = 10.49GVSLQLLEE162 pKa = 5.08HH163 pKa = 6.54NPALDD168 pKa = 3.63VITPRR173 pKa = 11.84KK174 pKa = 9.16KK175 pKa = 9.9QEE177 pKa = 3.84KK178 pKa = 6.84KK179 pKa = 9.19TKK181 pKa = 10.04IKK183 pKa = 10.59FLDD186 pKa = 3.65KK187 pKa = 11.22QEE189 pKa = 4.39LKK191 pKa = 10.84QFLSYY196 pKa = 11.3LDD198 pKa = 4.0TLDD201 pKa = 3.85KK202 pKa = 11.1SDD204 pKa = 3.94YY205 pKa = 10.77KK206 pKa = 11.49NLFNTVLYY214 pKa = 10.51KK215 pKa = 11.0LLLATGLRR223 pKa = 11.84IGEE226 pKa = 4.09AMALEE231 pKa = 4.4WSDD234 pKa = 3.48VDD236 pKa = 5.19LEE238 pKa = 4.38NGVIDD243 pKa = 4.94VNKK246 pKa = 9.18TLNKK250 pKa = 9.83RR251 pKa = 11.84IEE253 pKa = 4.22INSPKK258 pKa = 10.86SMASYY263 pKa = 10.18RR264 pKa = 11.84QISIDD269 pKa = 3.08KK270 pKa = 8.25TTILMLKK277 pKa = 9.46QYY279 pKa = 11.14KK280 pKa = 9.7NRR282 pKa = 11.84QQVKK286 pKa = 8.05AWEE289 pKa = 4.27LGRR292 pKa = 11.84SEE294 pKa = 5.68KK295 pKa = 10.4IVFSNFTGKK304 pKa = 10.45YY305 pKa = 9.22FDD307 pKa = 4.9PNNIRR312 pKa = 11.84NQLYY316 pKa = 10.08KK317 pKa = 10.2HH318 pKa = 6.4FKK320 pKa = 8.94NAGVPNVRR328 pKa = 11.84FHH330 pKa = 6.96GLRR333 pKa = 11.84HH334 pKa = 4.55THH336 pKa = 5.68ATLMLNAGMSPKK348 pKa = 10.41DD349 pKa = 3.41LQHH352 pKa = 6.96RR353 pKa = 11.84LGHH356 pKa = 5.16STITMTLNIYY366 pKa = 9.09VHH368 pKa = 5.88ATEE371 pKa = 5.13EE372 pKa = 4.44GAKK375 pKa = 9.92QGGNIFEE382 pKa = 4.18MAINNLL388 pKa = 3.61

Molecular weight: 44.59 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

12

0

12

2027

41

476

168.9

19.68

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

4.539 ± 0.658

0.395 ± 0.171

6.66 ± 0.759

7.844 ± 0.802

4.489 ± 0.554

4.44 ± 0.48

1.332 ± 0.277

7.203 ± 0.38

10.163 ± 0.632

9.423 ± 0.826

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.22 ± 0.327

7.252 ± 0.522

2.467 ± 0.225

3.799 ± 0.261

4.539 ± 0.639

5.081 ± 0.512

5.871 ± 0.549

6.216 ± 0.492

0.987 ± 0.166

5.081 ± 0.412
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski