Burkholderia virus phiE125
Average proteome isoelectric point is 6.74
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 72 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|Q8W6R1|Q8W6R1_9CAUD TnpA OS=Burkholderia virus phiE125 OX=180504 GN=tnpA PE=4 SV=1
MM1 pKa = 7.35 NVTDD5 pKa = 3.97 KK6 pKa = 11.35 TKK8 pKa = 10.98 GDD10 pKa = 3.4 TSADD14 pKa = 3.37 VTRR17 pKa = 11.84 TVWSKK22 pKa = 11.35 DD23 pKa = 3.28 NEE25 pKa = 4.34 FFSCDD30 pKa = 3.62 ALDD33 pKa = 4.36 EE34 pKa = 4.51 LLDD37 pKa = 3.68 MHH39 pKa = 7.54 DD40 pKa = 4.03 EE41 pKa = 4.13 LAVGDD46 pKa = 3.95 VVFYY50 pKa = 11.27 GDD52 pKa = 3.53 VAPIPTEE59 pKa = 4.02 RR60 pKa = 11.84 LCDD63 pKa = 3.92 ADD65 pKa = 5.75 DD66 pKa = 4.61 VIDD69 pKa = 4.36 MIGEE73 pKa = 4.11 RR74 pKa = 11.84 AYY76 pKa = 11.03 DD77 pKa = 3.91 EE78 pKa = 4.35 VGEE81 pKa = 4.41 AADD84 pKa = 5.22 GYY86 pKa = 10.37 PDD88 pKa = 3.56 IAPEE92 pKa = 3.99 AKK94 pKa = 10.54 AEE96 pKa = 4.1 LEE98 pKa = 4.22 TLLSGWIEE106 pKa = 3.93 KK107 pKa = 9.34 HH108 pKa = 6.08 AKK110 pKa = 7.05 PTFYY114 pKa = 10.83 SAANVNEE121 pKa = 4.04 HH122 pKa = 6.7 VITEE126 pKa = 4.23 ANVEE130 pKa = 4.08 GRR132 pKa = 11.84 EE133 pKa = 4.09
Molecular weight: 14.63 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 4.174
IPC2_protein 3.973
IPC_protein 3.948
Toseland 3.745
ProMoST 4.062
Dawson 3.923
Bjellqvist 4.113
Wikipedia 3.834
Rodwell 3.77
Grimsley 3.656
Solomon 3.91
Lehninger 3.872
Nozaki 4.037
DTASelect 4.24
Thurlkill 3.783
EMBOSS 3.846
Sillero 4.062
Patrickios 3.452
IPC_peptide 3.91
IPC2_peptide 4.037
IPC2.peptide.svr19 3.983
Protein with the highest isoelectric point:
>tr|Q8W6R7|Q8W6R7_9CAUD Integrase OS=Burkholderia virus phiE125 OX=180504 GN=34 PE=3 SV=1
MM1 pKa = 7.92 RR2 pKa = 11.84 LPRR5 pKa = 11.84 QTRR8 pKa = 11.84 ARR10 pKa = 11.84 RR11 pKa = 11.84 AAVSSHH17 pKa = 6.02 WISRR21 pKa = 11.84 KK22 pKa = 8.68 PRR24 pKa = 11.84 SGFKK28 pKa = 10.1 DD29 pKa = 3.11 RR30 pKa = 11.84 SMNEE34 pKa = 3.84 RR35 pKa = 11.84 LMTPADD41 pKa = 3.7 LTVVTGKK48 pKa = 10.18 KK49 pKa = 9.98 RR50 pKa = 11.84 YY51 pKa = 7.1 TKK53 pKa = 10.17 QADD56 pKa = 3.12 WFKK59 pKa = 10.67 ATFGINVVTAANGAVIMTWSTFEE82 pKa = 3.92 SLQAKK87 pKa = 9.66 KK88 pKa = 10.54 AGLVGNPEE96 pKa = 3.96 TSTVEE101 pKa = 3.95 LCFDD105 pKa = 3.77
Molecular weight: 11.78 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.307
IPC2_protein 9.677
IPC_protein 10.292
Toseland 10.979
ProMoST 10.643
Dawson 11.023
Bjellqvist 10.716
Wikipedia 11.228
Rodwell 11.316
Grimsley 11.052
Solomon 11.184
Lehninger 11.155
Nozaki 10.95
DTASelect 10.701
Thurlkill 10.95
EMBOSS 11.389
Sillero 10.965
Patrickios 11.067
IPC_peptide 11.199
IPC2_peptide 9.531
IPC2.peptide.svr19 8.782
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
72
0
72
16002
43
1354
222.3
24.4
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
11.936 ± 0.798
1.181 ± 0.174
6.062 ± 0.198
6.143 ± 0.32
3.156 ± 0.179
8.086 ± 0.304
1.694 ± 0.168
4.706 ± 0.219
4.256 ± 0.26
7.937 ± 0.29
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.381 ± 0.123
3.287 ± 0.16
4.718 ± 0.362
4.056 ± 0.269
7.818 ± 0.304
5.881 ± 0.249
5.162 ± 0.205
7.28 ± 0.307
1.675 ± 0.16
2.587 ± 0.199
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here