Murine polyomavirus (strain A3) (MPyV)

Taxonomy: Viruses; Monodnaviria; Shotokuvirae; Cossaviricota; Papovaviricetes; Sepolyvirales; Polyomaviridae; Alphapolyomavirus; Mus musculus polyomavirus 1

Average proteome isoelectric point is 6.33

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 6 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>sp|P0DOJ5|LT_POVM3 Large T antigen OS=Murine polyomavirus (strain A3) OX=157703 PE=1 SV=1
MM1 pKa = 7.22GAALTILVDD10 pKa = 5.25LIEE13 pKa = 4.62GLAEE17 pKa = 4.01VSTLTGLSAEE27 pKa = 4.95AILSGEE33 pKa = 4.18ALAALDD39 pKa = 4.41GEE41 pKa = 4.62ITALTLEE48 pKa = 4.75GVMSSEE54 pKa = 4.09TALATMGISEE64 pKa = 4.38EE65 pKa = 4.53VYY67 pKa = 10.09GFVSTVPVFVNRR79 pKa = 11.84TAGAIWLMQTVQGASTISLGIQRR102 pKa = 11.84YY103 pKa = 6.9LHH105 pKa = 5.99NEE107 pKa = 3.6EE108 pKa = 4.36VPTVNRR114 pKa = 11.84NMALIPWRR122 pKa = 11.84DD123 pKa = 3.35PALLDD128 pKa = 3.47IYY130 pKa = 10.9FPGVNQFAHH139 pKa = 6.9ALNVVHH145 pKa = 7.45DD146 pKa = 4.14WGHH149 pKa = 5.87GLLHH153 pKa = 6.21SVGRR157 pKa = 11.84YY158 pKa = 4.99VWQMVVQEE166 pKa = 4.3TQHH169 pKa = 6.79RR170 pKa = 11.84LEE172 pKa = 4.31GAVRR176 pKa = 11.84EE177 pKa = 4.19LTVRR181 pKa = 11.84QTHH184 pKa = 5.6TFLDD188 pKa = 4.04GLARR192 pKa = 11.84LLEE195 pKa = 3.97NTRR198 pKa = 11.84WVVSNAPQSAIDD210 pKa = 4.41AINRR214 pKa = 11.84GASSVSSGYY223 pKa = 10.78SSLSDD228 pKa = 3.48YY229 pKa = 10.74YY230 pKa = 10.85RR231 pKa = 11.84QLGLNPPQRR240 pKa = 11.84RR241 pKa = 11.84ALFNRR246 pKa = 11.84IEE248 pKa = 4.06GSMGNGGPTPAAHH261 pKa = 6.53IQDD264 pKa = 3.67EE265 pKa = 4.48SGEE268 pKa = 4.32VIKK271 pKa = 10.28FYY273 pKa = 10.31QAPGGAHH280 pKa = 5.59QRR282 pKa = 11.84VTPDD286 pKa = 2.33WMLPLILGLYY296 pKa = 10.53GDD298 pKa = 4.76ITPTWATVIEE308 pKa = 4.19EE309 pKa = 5.22DD310 pKa = 4.7GPQKK314 pKa = 10.47KK315 pKa = 9.35KK316 pKa = 10.6RR317 pKa = 11.84RR318 pKa = 11.84LL319 pKa = 3.34

Molecular weight: 34.72 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|Q84243|Q84243_POVM3 Capsid protein VP1 OS=Murine polyomavirus (strain A3) OX=157703 PE=3 SV=2
MM1 pKa = 7.9DD2 pKa = 4.81RR3 pKa = 11.84VLSRR7 pKa = 11.84ADD9 pKa = 3.34KK10 pKa = 10.73EE11 pKa = 4.2RR12 pKa = 11.84LLEE15 pKa = 4.0LLKK18 pKa = 10.85LPRR21 pKa = 11.84QLWGDD26 pKa = 4.15FGRR29 pKa = 11.84MQQAYY34 pKa = 8.36KK35 pKa = 10.05QQSLLLHH42 pKa = 6.5PDD44 pKa = 2.84KK45 pKa = 11.27GGSHH49 pKa = 7.21ALMQEE54 pKa = 4.36LNSLWGTFKK63 pKa = 10.89TEE65 pKa = 3.95VYY67 pKa = 9.97NLRR70 pKa = 11.84MNLGGTGFQVRR81 pKa = 11.84RR82 pKa = 11.84LHH84 pKa = 6.78ADD86 pKa = 2.98GWNLSTKK93 pKa = 9.7DD94 pKa = 3.51TFGDD98 pKa = 3.34RR99 pKa = 11.84YY100 pKa = 8.3YY101 pKa = 11.23QRR103 pKa = 11.84FCRR106 pKa = 11.84MPLTCLVNVKK116 pKa = 10.13YY117 pKa = 10.71SSCSCILCLLRR128 pKa = 11.84KK129 pKa = 7.28QHH131 pKa = 7.15RR132 pKa = 11.84EE133 pKa = 3.97LKK135 pKa = 9.99DD136 pKa = 3.19KK137 pKa = 11.1CDD139 pKa = 3.54ARR141 pKa = 11.84CLVLGEE147 pKa = 4.41CFCLEE152 pKa = 5.76CYY154 pKa = 7.96MQWFGTPTRR163 pKa = 11.84DD164 pKa = 4.22VLNLYY169 pKa = 10.81ADD171 pKa = 6.04FIASMPIDD179 pKa = 3.67WLDD182 pKa = 3.98LDD184 pKa = 3.83VHH186 pKa = 6.38SVYY189 pKa = 10.77NPRR192 pKa = 11.84LSPP195 pKa = 3.73

Molecular weight: 22.81 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

5

1

6

2305

195

782

384.2

43.21

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

5.813 ± 0.713

2.299 ± 0.5

5.38 ± 0.273

5.944 ± 0.357

3.514 ± 0.596

7.245 ± 0.588

2.646 ± 0.369

3.341 ± 0.468

4.642 ± 0.993

11.8 ± 1.087

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.777 ± 0.385

3.731 ± 0.433

6.334 ± 0.504

4.685 ± 0.334

6.117 ± 0.868

6.855 ± 0.697

5.944 ± 0.859

5.9 ± 0.857

1.735 ± 0.229

3.297 ± 0.134
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski