Escherichia phage HK639

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; unclassified Siphoviridae

Average proteome isoelectric point is 6.4

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 76 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|G8C7R2|G8C7R2_9CAUD Tail assembly protein K OS=Escherichia phage HK639 OX=906669 GN=HK639_21 PE=3 SV=1
MM1 pKa = 7.49ALVDD5 pKa = 4.07QAAMLAPGGRR15 pKa = 11.84VRR17 pKa = 11.84LVEE20 pKa = 3.82VDD22 pKa = 3.27ASEE25 pKa = 4.24FSGGIHH31 pKa = 6.57RR32 pKa = 11.84FHH34 pKa = 6.96YY35 pKa = 10.81APFPHH40 pKa = 6.53TPEE43 pKa = 5.07EE44 pKa = 3.81IDD46 pKa = 3.4AANGDD51 pKa = 4.03EE52 pKa = 4.43QKK54 pKa = 10.83LGPKK58 pKa = 10.0PIFFGGNTYY67 pKa = 10.72DD68 pKa = 3.68FWPFQVSGLEE78 pKa = 4.02LSTDD82 pKa = 3.46QAAEE86 pKa = 3.99PSLSVSNLDD95 pKa = 3.31GHH97 pKa = 6.19ITALCLQFKK106 pKa = 11.03DD107 pKa = 3.46MVNAKK112 pKa = 10.1VSIIDD117 pKa = 3.76TYY119 pKa = 11.61AVYY122 pKa = 11.02LDD124 pKa = 3.44AVNYY128 pKa = 10.39AGGVNPTADD137 pKa = 2.78SSMFTIQTFWLDD149 pKa = 3.56TKK151 pKa = 10.53TSEE154 pKa = 4.62DD155 pKa = 4.02DD156 pKa = 4.64EE157 pKa = 4.86VVTWSLSSPADD168 pKa = 3.63LQNLVIPTRR177 pKa = 11.84QITSLCEE184 pKa = 3.11WALRR188 pKa = 11.84GQYY191 pKa = 10.15RR192 pKa = 11.84SGDD195 pKa = 3.19GCTYY199 pKa = 11.02NGTAYY204 pKa = 10.46FDD206 pKa = 4.05AKK208 pKa = 10.23GNPVADD214 pKa = 4.26PALDD218 pKa = 3.54VCGGCLSDD226 pKa = 3.3CRR228 pKa = 11.84KK229 pKa = 10.26RR230 pKa = 11.84FGAGLADD237 pKa = 4.22PDD239 pKa = 4.55AAILDD244 pKa = 4.12FGGFPATVLFIRR256 pKa = 4.85

Molecular weight: 27.59 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|G8C7U8|G8C7U8_9CAUD Eae-like protein OS=Escherichia phage HK639 OX=906669 GN=HK639_57 PE=4 SV=1
MM1 pKa = 7.75TITIRR6 pKa = 11.84GQILAALRR14 pKa = 11.84NNPGLSSARR23 pKa = 11.84IATMIGMTTKK33 pKa = 10.48KK34 pKa = 10.23ISGPLSTLFADD45 pKa = 4.5GLIEE49 pKa = 4.44FEE51 pKa = 4.43GKK53 pKa = 9.66HH54 pKa = 4.55GQRR57 pKa = 11.84LYY59 pKa = 11.18RR60 pKa = 11.84LTSYY64 pKa = 10.33GMKK67 pKa = 9.88YY68 pKa = 10.57APEE71 pKa = 4.85TIPAMPKK78 pKa = 10.27GNSKK82 pKa = 10.27LVQRR86 pKa = 11.84TEE88 pKa = 4.21ANVTCQEE95 pKa = 4.32CRR97 pKa = 11.84NSPAMRR103 pKa = 11.84RR104 pKa = 11.84VLMVWGRR111 pKa = 11.84VGGG114 pKa = 3.72

Molecular weight: 12.48 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

76

0

76

14608

49

1154

192.2

21.22

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

9.988 ± 0.406

0.965 ± 0.157

5.778 ± 0.21

6.106 ± 0.326

3.621 ± 0.179

7.674 ± 0.323

1.711 ± 0.181

5.743 ± 0.236

5.34 ± 0.266

7.79 ± 0.28

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.903 ± 0.141

4.299 ± 0.199

4.135 ± 0.288

4.676 ± 0.24

5.518 ± 0.275

6.558 ± 0.21

6.079 ± 0.348

6.592 ± 0.246

1.616 ± 0.162

2.909 ± 0.154
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski