Amino acid dipepetide frequency for Escherichia phage HK639

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
11.501AlaAla: 11.501 ± 1.557
0.89AlaCys: 0.89 ± 0.257
6.093AlaAsp: 6.093 ± 0.581
6.23AlaGlu: 6.23 ± 0.778
3.286AlaPhe: 3.286 ± 0.512
8.284AlaGly: 8.284 ± 1.001
1.506AlaHis: 1.506 ± 0.325
5.888AlaIle: 5.888 ± 0.578
4.587AlaLys: 4.587 ± 0.611
6.983AlaLeu: 6.983 ± 0.622
3.491AlaMet: 3.491 ± 0.502
3.697AlaAsn: 3.697 ± 0.388
2.875AlaPro: 2.875 ± 0.537
4.45AlaGln: 4.45 ± 0.598
4.587AlaArg: 4.587 ± 0.674
7.394AlaSer: 7.394 ± 0.625
5.614AlaThr: 5.614 ± 0.597
7.736AlaVal: 7.736 ± 0.757
1.985AlaTrp: 1.985 ± 0.287
2.807AlaTyr: 2.807 ± 0.431
0.0AlaXaa: 0.0 ± 0.0
Cys
0.958CysAla: 0.958 ± 0.297
0.205CysCys: 0.205 ± 0.114
0.274CysAsp: 0.274 ± 0.132
0.616CysGlu: 0.616 ± 0.193
0.205CysPhe: 0.205 ± 0.119
0.89CysGly: 0.89 ± 0.231
0.274CysHis: 0.274 ± 0.151
0.479CysIle: 0.479 ± 0.173
0.548CysLys: 0.548 ± 0.204
0.753CysLeu: 0.753 ± 0.224
0.479CysMet: 0.479 ± 0.192
0.411CysAsn: 0.411 ± 0.159
0.685CysPro: 0.685 ± 0.216
0.411CysGln: 0.411 ± 0.132
0.89CysArg: 0.89 ± 0.227
0.548CysSer: 0.548 ± 0.196
0.411CysThr: 0.411 ± 0.159
0.411CysVal: 0.411 ± 0.204
0.137CysTrp: 0.137 ± 0.085
0.068CysTyr: 0.068 ± 0.07
0.0CysXaa: 0.0 ± 0.0
Asp
6.298AspAla: 6.298 ± 0.577
0.685AspCys: 0.685 ± 0.236
3.765AspAsp: 3.765 ± 0.474
3.355AspGlu: 3.355 ± 0.4
2.054AspPhe: 2.054 ± 0.353
6.298AspGly: 6.298 ± 0.679
0.479AspHis: 0.479 ± 0.217
2.807AspIle: 2.807 ± 0.409
2.67AspLys: 2.67 ± 0.404
4.176AspLeu: 4.176 ± 0.485
1.643AspMet: 1.643 ± 0.297
2.054AspAsn: 2.054 ± 0.317
2.122AspPro: 2.122 ± 0.268
2.533AspGln: 2.533 ± 0.489
3.423AspArg: 3.423 ± 0.499
3.834AspSer: 3.834 ± 0.514
2.601AspThr: 2.601 ± 0.513
3.765AspVal: 3.765 ± 0.553
1.095AspTrp: 1.095 ± 0.27
2.122AspTyr: 2.122 ± 0.363
0.0AspXaa: 0.0 ± 0.0
Glu
6.367GluAla: 6.367 ± 1.234
0.89GluCys: 0.89 ± 0.272
2.738GluAsp: 2.738 ± 0.406
3.56GluGlu: 3.56 ± 0.435
2.738GluPhe: 2.738 ± 0.359
3.491GluGly: 3.491 ± 0.497
1.301GluHis: 1.301 ± 0.304
3.012GluIle: 3.012 ± 0.462
2.944GluLys: 2.944 ± 0.481
5.545GluLeu: 5.545 ± 0.603
2.396GluMet: 2.396 ± 0.413
3.012GluAsn: 3.012 ± 0.463
2.122GluPro: 2.122 ± 0.377
3.491GluGln: 3.491 ± 0.673
3.355GluArg: 3.355 ± 0.634
3.56GluSer: 3.56 ± 0.508
2.807GluThr: 2.807 ± 0.456
4.108GluVal: 4.108 ± 0.468
1.164GluTrp: 1.164 ± 0.273
2.465GluTyr: 2.465 ± 0.37
0.0GluXaa: 0.0 ± 0.0
Phe
2.601PheAla: 2.601 ± 0.309
0.479PheCys: 0.479 ± 0.186
3.218PheAsp: 3.218 ± 0.442
1.848PheGlu: 1.848 ± 0.287
1.095PhePhe: 1.095 ± 0.299
3.355PheGly: 3.355 ± 0.523
0.753PheHis: 0.753 ± 0.184
2.259PheIle: 2.259 ± 0.443
1.78PheLys: 1.78 ± 0.351
2.191PheLeu: 2.191 ± 0.361
0.822PheMet: 0.822 ± 0.254
2.259PheAsn: 2.259 ± 0.359
1.643PhePro: 1.643 ± 0.382
1.095PheGln: 1.095 ± 0.238
1.848PheArg: 1.848 ± 0.395
3.081PheSer: 3.081 ± 0.427
2.259PheThr: 2.259 ± 0.373
1.643PheVal: 1.643 ± 0.31
0.548PheTrp: 0.548 ± 0.233
1.438PheTyr: 1.438 ± 0.326
0.0PheXaa: 0.0 ± 0.0
Gly
5.956GlyAla: 5.956 ± 0.91
0.958GlyCys: 0.958 ± 0.222
4.245GlyAsp: 4.245 ± 0.382
4.724GlyGlu: 4.724 ± 0.644
2.67GlyPhe: 2.67 ± 0.411
7.531GlyGly: 7.531 ± 1.163
0.89GlyHis: 0.89 ± 0.256
4.587GlyIle: 4.587 ± 0.52
5.751GlyLys: 5.751 ± 0.679
7.12GlyLeu: 7.12 ± 0.766
2.465GlyMet: 2.465 ± 0.397
3.355GlyAsn: 3.355 ± 0.457
1.438GlyPro: 1.438 ± 0.301
3.081GlyGln: 3.081 ± 0.414
3.765GlyArg: 3.765 ± 0.484
4.518GlySer: 4.518 ± 0.685
4.655GlyThr: 4.655 ± 0.698
5.408GlyVal: 5.408 ± 0.519
1.643GlyTrp: 1.643 ± 0.316
1.985GlyTyr: 1.985 ± 0.285
0.0GlyXaa: 0.0 ± 0.0
His
1.78HisAla: 1.78 ± 0.373
0.205HisCys: 0.205 ± 0.096
1.164HisAsp: 1.164 ± 0.332
1.438HisGlu: 1.438 ± 0.382
0.753HisPhe: 0.753 ± 0.248
1.369HisGly: 1.369 ± 0.405
0.342HisHis: 0.342 ± 0.149
0.958HisIle: 0.958 ± 0.225
0.685HisLys: 0.685 ± 0.217
1.027HisLeu: 1.027 ± 0.316
0.411HisMet: 0.411 ± 0.188
0.548HisAsn: 0.548 ± 0.185
0.822HisPro: 0.822 ± 0.271
0.753HisGln: 0.753 ± 0.263
1.232HisArg: 1.232 ± 0.296
0.958HisSer: 0.958 ± 0.243
1.164HisThr: 1.164 ± 0.314
0.548HisVal: 0.548 ± 0.205
0.205HisTrp: 0.205 ± 0.109
0.753HisTyr: 0.753 ± 0.192
0.0HisXaa: 0.0 ± 0.0
Ile
5.477IleAla: 5.477 ± 0.611
0.753IleCys: 0.753 ± 0.284
4.655IleAsp: 4.655 ± 0.571
3.355IleGlu: 3.355 ± 0.557
1.301IlePhe: 1.301 ± 0.289
3.697IleGly: 3.697 ± 0.483
0.958IleHis: 0.958 ± 0.215
3.012IleIle: 3.012 ± 0.496
3.628IleLys: 3.628 ± 0.453
3.491IleLeu: 3.491 ± 0.409
1.917IleMet: 1.917 ± 0.362
2.465IleAsn: 2.465 ± 0.423
3.218IlePro: 3.218 ± 0.465
2.738IleGln: 2.738 ± 0.579
3.149IleArg: 3.149 ± 0.418
4.176IleSer: 4.176 ± 0.518
4.861IleThr: 4.861 ± 0.629
2.465IleVal: 2.465 ± 0.389
0.479IleTrp: 0.479 ± 0.155
1.643IleTyr: 1.643 ± 0.265
0.0IleXaa: 0.0 ± 0.0
Lys
5.408LysAla: 5.408 ± 0.55
0.205LysCys: 0.205 ± 0.113
2.122LysAsp: 2.122 ± 0.308
3.834LysGlu: 3.834 ± 0.537
1.643LysPhe: 1.643 ± 0.349
3.628LysGly: 3.628 ± 0.511
1.164LysHis: 1.164 ± 0.282
2.67LysIle: 2.67 ± 0.391
2.67LysLys: 2.67 ± 0.462
4.518LysLeu: 4.518 ± 0.499
1.712LysMet: 1.712 ± 0.304
2.67LysAsn: 2.67 ± 0.415
3.56LysPro: 3.56 ± 0.514
2.601LysGln: 2.601 ± 0.38
3.286LysArg: 3.286 ± 0.356
2.944LysSer: 2.944 ± 0.506
3.355LysThr: 3.355 ± 0.481
3.286LysVal: 3.286 ± 0.549
0.89LysTrp: 0.89 ± 0.236
1.232LysTyr: 1.232 ± 0.263
0.0LysXaa: 0.0 ± 0.0
Leu
8.694LeuAla: 8.694 ± 0.668
0.479LeuCys: 0.479 ± 0.199
4.655LeuAsp: 4.655 ± 0.633
5.203LeuGlu: 5.203 ± 0.628
3.218LeuPhe: 3.218 ± 0.463
3.902LeuGly: 3.902 ± 0.541
1.232LeuHis: 1.232 ± 0.267
4.724LeuIle: 4.724 ± 0.384
3.971LeuLys: 3.971 ± 0.515
5.956LeuLeu: 5.956 ± 0.813
1.712LeuMet: 1.712 ± 0.327
3.971LeuAsn: 3.971 ± 0.569
2.944LeuPro: 2.944 ± 0.389
3.491LeuGln: 3.491 ± 0.575
5.408LeuArg: 5.408 ± 0.617
5.477LeuSer: 5.477 ± 0.582
5.203LeuThr: 5.203 ± 0.563
4.176LeuVal: 4.176 ± 0.596
0.822LeuTrp: 0.822 ± 0.223
2.67LeuTyr: 2.67 ± 0.432
0.0LeuXaa: 0.0 ± 0.0
Met
3.149MetAla: 3.149 ± 0.407
0.548MetCys: 0.548 ± 0.234
0.89MetAsp: 0.89 ± 0.214
1.164MetGlu: 1.164 ± 0.264
0.89MetPhe: 0.89 ± 0.27
2.191MetGly: 2.191 ± 0.405
0.548MetHis: 0.548 ± 0.222
1.78MetIle: 1.78 ± 0.339
2.328MetLys: 2.328 ± 0.364
1.917MetLeu: 1.917 ± 0.335
0.753MetMet: 0.753 ± 0.236
1.438MetAsn: 1.438 ± 0.282
1.506MetPro: 1.506 ± 0.246
1.712MetGln: 1.712 ± 0.278
1.438MetArg: 1.438 ± 0.34
2.054MetSer: 2.054 ± 0.3
2.054MetThr: 2.054 ± 0.372
2.191MetVal: 2.191 ± 0.381
0.205MetTrp: 0.205 ± 0.125
0.274MetTyr: 0.274 ± 0.131
0.0MetXaa: 0.0 ± 0.0
Asn
3.971AsnAla: 3.971 ± 0.496
0.274AsnCys: 0.274 ± 0.109
2.122AsnAsp: 2.122 ± 0.372
2.465AsnGlu: 2.465 ± 0.351
1.369AsnPhe: 1.369 ± 0.257
4.245AsnGly: 4.245 ± 0.583
0.958AsnHis: 0.958 ± 0.263
2.807AsnIle: 2.807 ± 0.375
2.191AsnLys: 2.191 ± 0.331
2.875AsnLeu: 2.875 ± 0.51
1.232AsnMet: 1.232 ± 0.274
1.712AsnAsn: 1.712 ± 0.477
3.012AsnPro: 3.012 ± 0.486
2.191AsnGln: 2.191 ± 0.425
1.438AsnArg: 1.438 ± 0.345
2.807AsnSer: 2.807 ± 0.532
2.396AsnThr: 2.396 ± 0.375
2.875AsnVal: 2.875 ± 0.436
0.89AsnTrp: 0.89 ± 0.246
1.164AsnTyr: 1.164 ± 0.263
0.0AsnXaa: 0.0 ± 0.0
Pro
5.135ProAla: 5.135 ± 0.529
0.205ProCys: 0.205 ± 0.097
3.423ProAsp: 3.423 ± 0.672
3.149ProGlu: 3.149 ± 0.438
1.78ProPhe: 1.78 ± 0.324
3.765ProGly: 3.765 ± 0.481
0.89ProHis: 0.89 ± 0.265
1.575ProIle: 1.575 ± 0.323
2.122ProLys: 2.122 ± 0.386
3.081ProLeu: 3.081 ± 0.43
0.548ProMet: 0.548 ± 0.215
0.89ProAsn: 0.89 ± 0.26
1.575ProPro: 1.575 ± 0.37
2.054ProGln: 2.054 ± 0.344
1.712ProArg: 1.712 ± 0.445
2.328ProSer: 2.328 ± 0.401
2.328ProThr: 2.328 ± 0.343
2.944ProVal: 2.944 ± 0.446
0.753ProTrp: 0.753 ± 0.252
1.095ProTyr: 1.095 ± 0.252
0.0ProXaa: 0.0 ± 0.0
Gln
4.587GlnAla: 4.587 ± 0.547
0.616GlnCys: 0.616 ± 0.201
1.369GlnAsp: 1.369 ± 0.264
2.396GlnGlu: 2.396 ± 0.396
1.78GlnPhe: 1.78 ± 0.311
2.465GlnGly: 2.465 ± 0.384
1.027GlnHis: 1.027 ± 0.241
3.149GlnIle: 3.149 ± 0.464
3.149GlnLys: 3.149 ± 0.53
4.655GlnLeu: 4.655 ± 0.446
1.301GlnMet: 1.301 ± 0.202
1.985GlnAsn: 1.985 ± 0.378
1.78GlnPro: 1.78 ± 0.314
2.738GlnGln: 2.738 ± 0.55
2.122GlnArg: 2.122 ± 0.368
2.67GlnSer: 2.67 ± 0.398
3.081GlnThr: 3.081 ± 0.586
3.697GlnVal: 3.697 ± 0.517
0.753GlnTrp: 0.753 ± 0.271
1.438GlnTyr: 1.438 ± 0.286
0.0GlnXaa: 0.0 ± 0.0
Arg
4.998ArgAla: 4.998 ± 0.65
0.548ArgCys: 0.548 ± 0.18
2.601ArgAsp: 2.601 ± 0.49
3.56ArgGlu: 3.56 ± 0.512
1.848ArgPhe: 1.848 ± 0.427
3.697ArgGly: 3.697 ± 0.496
1.095ArgHis: 1.095 ± 0.339
3.355ArgIle: 3.355 ± 0.548
2.533ArgLys: 2.533 ± 0.441
5.408ArgLeu: 5.408 ± 0.555
1.848ArgMet: 1.848 ± 0.313
2.67ArgAsn: 2.67 ± 0.487
1.643ArgPro: 1.643 ± 0.363
2.67ArgGln: 2.67 ± 0.567
3.628ArgArg: 3.628 ± 0.631
2.533ArgSer: 2.533 ± 0.436
3.355ArgThr: 3.355 ± 0.491
4.655ArgVal: 4.655 ± 0.678
1.027ArgTrp: 1.027 ± 0.301
1.506ArgTyr: 1.506 ± 0.273
0.0ArgXaa: 0.0 ± 0.0
Ser
6.504SerAla: 6.504 ± 0.6
0.479SerCys: 0.479 ± 0.183
4.176SerAsp: 4.176 ± 0.517
3.491SerGlu: 3.491 ± 0.5
2.396SerPhe: 2.396 ± 0.374
5.751SerGly: 5.751 ± 0.49
0.958SerHis: 0.958 ± 0.246
2.396SerIle: 2.396 ± 0.356
3.286SerLys: 3.286 ± 0.541
5.545SerLeu: 5.545 ± 0.594
1.985SerMet: 1.985 ± 0.348
2.807SerAsn: 2.807 ± 0.486
2.875SerPro: 2.875 ± 0.405
3.149SerGln: 3.149 ± 0.482
3.355SerArg: 3.355 ± 0.43
4.45SerSer: 4.45 ± 0.528
4.313SerThr: 4.313 ± 0.516
4.929SerVal: 4.929 ± 0.535
1.164SerTrp: 1.164 ± 0.249
1.575SerTyr: 1.575 ± 0.311
0.0SerXaa: 0.0 ± 0.0
Thr
6.298ThrAla: 6.298 ± 0.846
0.205ThrCys: 0.205 ± 0.116
2.67ThrAsp: 2.67 ± 0.416
3.834ThrGlu: 3.834 ± 0.515
2.807ThrPhe: 2.807 ± 0.49
5.135ThrGly: 5.135 ± 0.534
1.232ThrHis: 1.232 ± 0.212
4.381ThrIle: 4.381 ± 0.542
3.697ThrLys: 3.697 ± 0.513
4.861ThrLeu: 4.861 ± 0.709
1.232ThrMet: 1.232 ± 0.33
1.985ThrAsn: 1.985 ± 0.343
3.56ThrPro: 3.56 ± 0.568
2.533ThrGln: 2.533 ± 0.555
3.012ThrArg: 3.012 ± 0.455
3.902ThrSer: 3.902 ± 0.47
2.807ThrThr: 2.807 ± 0.469
4.176ThrVal: 4.176 ± 0.49
0.616ThrTrp: 0.616 ± 0.207
1.848ThrTyr: 1.848 ± 0.4
0.0ThrXaa: 0.0 ± 0.0
Val
6.23ValAla: 6.23 ± 0.584
0.548ValCys: 0.548 ± 0.219
4.861ValAsp: 4.861 ± 0.499
4.039ValGlu: 4.039 ± 0.471
2.328ValPhe: 2.328 ± 0.327
3.423ValGly: 3.423 ± 0.49
0.958ValHis: 0.958 ± 0.293
4.998ValIle: 4.998 ± 0.617
3.012ValLys: 3.012 ± 0.419
4.45ValLeu: 4.45 ± 0.565
2.328ValMet: 2.328 ± 0.424
3.012ValAsn: 3.012 ± 0.541
2.328ValPro: 2.328 ± 0.434
2.396ValGln: 2.396 ± 0.387
4.587ValArg: 4.587 ± 0.553
4.998ValSer: 4.998 ± 0.635
4.587ValThr: 4.587 ± 0.501
3.697ValVal: 3.697 ± 0.566
1.232ValTrp: 1.232 ± 0.287
1.917ValTyr: 1.917 ± 0.31
0.0ValXaa: 0.0 ± 0.0
Trp
1.027TrpAla: 1.027 ± 0.216
0.274TrpCys: 0.274 ± 0.139
1.027TrpAsp: 1.027 ± 0.296
1.506TrpGlu: 1.506 ± 0.325
0.479TrpPhe: 0.479 ± 0.184
1.301TrpGly: 1.301 ± 0.281
0.274TrpHis: 0.274 ± 0.131
0.822TrpIle: 0.822 ± 0.197
0.822TrpLys: 0.822 ± 0.196
1.506TrpLeu: 1.506 ± 0.335
0.205TrpMet: 0.205 ± 0.131
0.616TrpAsn: 0.616 ± 0.155
0.548TrpPro: 0.548 ± 0.18
1.095TrpGln: 1.095 ± 0.206
0.822TrpArg: 0.822 ± 0.223
1.164TrpSer: 1.164 ± 0.299
0.822TrpThr: 0.822 ± 0.237
1.095TrpVal: 1.095 ± 0.336
0.205TrpTrp: 0.205 ± 0.105
0.479TrpTyr: 0.479 ± 0.163
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.944TyrAla: 2.944 ± 0.48
0.205TyrCys: 0.205 ± 0.126
1.712TyrAsp: 1.712 ± 0.294
1.301TyrGlu: 1.301 ± 0.246
1.575TyrPhe: 1.575 ± 0.333
2.328TyrGly: 2.328 ± 0.454
0.274TyrHis: 0.274 ± 0.126
1.78TyrIle: 1.78 ± 0.334
1.027TyrLys: 1.027 ± 0.275
1.848TyrLeu: 1.848 ± 0.35
0.479TyrMet: 0.479 ± 0.171
1.438TyrAsn: 1.438 ± 0.305
1.095TyrPro: 1.095 ± 0.288
1.575TyrGln: 1.575 ± 0.334
2.122TyrArg: 2.122 ± 0.369
2.191TyrSer: 2.191 ± 0.424
2.122TyrThr: 2.122 ± 0.401
2.122TyrVal: 2.122 ± 0.3
0.342TyrTrp: 0.342 ± 0.156
0.616TyrTyr: 0.616 ± 0.168
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 76 proteins (14608 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski