Desulfitobacterium metallireducens DSM 15288

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Firmicutes; Clostridia; Eubacteriales; Peptococcaceae; Desulfitobacterium; Desulfitobacterium metallireducens

Average proteome isoelectric point is 6.36

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3029 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|W0E7N7|W0E7N7_9FIRM Sporulation protein Spo0E OS=Desulfitobacterium metallireducens DSM 15288 OX=871968 GN=DESME_06655 PE=4 SV=1
MM1 pKa = 7.28TLDD4 pKa = 3.32EE5 pKa = 5.24SKK7 pKa = 11.22NEE9 pKa = 3.72NDD11 pKa = 3.75IVDD14 pKa = 3.64EE15 pKa = 4.57GFGVTILTEE24 pKa = 4.51DD25 pKa = 3.53KK26 pKa = 10.12LTEE29 pKa = 4.08YY30 pKa = 11.22LEE32 pKa = 4.54GAVIDD37 pKa = 4.21YY38 pKa = 11.04VEE40 pKa = 4.1SQYY43 pKa = 11.88GGGFEE48 pKa = 4.82IKK50 pKa = 9.51TNNPGGGCDD59 pKa = 4.17SGCSSCGSS67 pKa = 3.39

Molecular weight: 7.06 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|W0E4M1|W0E4M1_9FIRM DNA gyrase subunit B OS=Desulfitobacterium metallireducens DSM 15288 OX=871968 GN=gyrB PE=3 SV=1
MM1 pKa = 7.36KK2 pKa = 9.44RR3 pKa = 11.84TYY5 pKa = 9.19QPKK8 pKa = 8.78NRR10 pKa = 11.84RR11 pKa = 11.84HH12 pKa = 5.44KK13 pKa = 10.09RR14 pKa = 11.84VHH16 pKa = 5.93GFLSRR21 pKa = 11.84MSSATGRR28 pKa = 11.84NVLKK32 pKa = 10.53RR33 pKa = 11.84RR34 pKa = 11.84RR35 pKa = 11.84LKK37 pKa = 10.68GRR39 pKa = 11.84KK40 pKa = 8.84KK41 pKa = 10.59LSAA44 pKa = 3.95

Molecular weight: 5.29 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3029

0

3029

896991

27

2217

296.1

33.0

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.489 ± 0.049

0.975 ± 0.02

4.733 ± 0.031

7.135 ± 0.058

4.108 ± 0.034

7.377 ± 0.046

1.898 ± 0.021

7.413 ± 0.038

6.15 ± 0.043

10.519 ± 0.058

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.58 ± 0.022

4.132 ± 0.045

4.023 ± 0.031

4.166 ± 0.034

4.561 ± 0.04

6.049 ± 0.036

5.329 ± 0.047

7.113 ± 0.043

1.06 ± 0.019

3.189 ± 0.027
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski