Planctomycetaceae bacterium SCGC AG-212-D15

Taxonomy: cellular organisms; Bacteria; PVC group; Planctomycetes; Planctomycetia; Planctomycetales; Planctomycetaceae; unclassified Planctomycetaceae

Average proteome isoelectric point is 6.98

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 1256 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A177PVT9|A0A177PVT9_9PLAN AAA domain-containing protein OS=Planctomycetaceae bacterium SCGC AG-212-D15 OX=1799653 GN=AYO40_05775 PE=4 SV=1
MM1 pKa = 7.4RR2 pKa = 11.84HH3 pKa = 5.99CFAILLRR10 pKa = 11.84LLLLLPLAPFLGCDD24 pKa = 3.41NGQVSPQVAYY34 pKa = 9.99IPVNPAQNNGVPFQVIVLIDD54 pKa = 3.77YY55 pKa = 10.51DD56 pKa = 4.66YY57 pKa = 11.88DD58 pKa = 6.22DD59 pKa = 6.71DD60 pKa = 7.53DD61 pKa = 7.52DD62 pKa = 7.43DD63 pKa = 7.18DD64 pKa = 4.62DD65 pKa = 4.23

Molecular weight: 7.28 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A177PWL6|A0A177PWL6_9PLAN KTSC domain-containing protein OS=Planctomycetaceae bacterium SCGC AG-212-D15 OX=1799653 GN=AYO40_01055 PE=4 SV=1
MM1 pKa = 7.04MVGIFSGCAWFLTFLVCHH19 pKa = 7.07LALFHH24 pKa = 6.31WRR26 pKa = 11.84PLRR29 pKa = 11.84NRR31 pKa = 11.84FKK33 pKa = 10.77AIARR37 pKa = 11.84IFMTAALGHH46 pKa = 6.53GLTALVLTPRR56 pKa = 11.84GPGLHH61 pKa = 6.65RR62 pKa = 11.84EE63 pKa = 4.09PVVWVAFISGWLVMACLFVLYY84 pKa = 9.8MPFFFSIGTSLSIQTMILVEE104 pKa = 4.53RR105 pKa = 11.84SANGRR110 pKa = 11.84AAVEE114 pKa = 4.08DD115 pKa = 3.83LRR117 pKa = 11.84EE118 pKa = 4.18AFASKK123 pKa = 10.51QLVAGRR129 pKa = 11.84LEE131 pKa = 4.1TMVANGYY138 pKa = 7.75LVCEE142 pKa = 4.2ADD144 pKa = 3.86RR145 pKa = 11.84YY146 pKa = 10.39RR147 pKa = 11.84VTAKK151 pKa = 10.25GRR153 pKa = 11.84LVAAVFGCLKK163 pKa = 10.26RR164 pKa = 11.84AWRR167 pKa = 11.84LGAGGG172 pKa = 3.58

Molecular weight: 19.04 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

1256

0

1256

386727

58

2944

307.9

33.91

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

10.758 ± 0.083

1.315 ± 0.031

5.52 ± 0.054

5.897 ± 0.068

3.616 ± 0.046

8.05 ± 0.072

2.307 ± 0.036

4.361 ± 0.043

4.155 ± 0.089

10.384 ± 0.088

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.151 ± 0.032

2.775 ± 0.063

5.723 ± 0.053

3.461 ± 0.046

7.493 ± 0.084

5.358 ± 0.056

5.141 ± 0.076

7.404 ± 0.058

1.651 ± 0.032

2.48 ± 0.047
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski