Fulvivirga sp. M361
Average proteome isoelectric point is 6.28
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 6272 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A553FFQ2|A0A553FFQ2_9BACT NADH-quinone oxidoreductase subunit H OS=Fulvivirga sp. M361 OX=2594266 GN=nuoH PE=3 SV=1
MM1 pKa = 7.32 SHH3 pKa = 5.92 TEE5 pKa = 3.78 LRR7 pKa = 11.84 RR8 pKa = 11.84 VCLLLIFVVGSFSNLLAQSYY28 pKa = 10.94 SGINWYY34 pKa = 10.1 FGNNDD39 pKa = 2.5 KK40 pKa = 10.91 SIRR43 pKa = 11.84 FTRR46 pKa = 11.84 PTLAPDD52 pKa = 3.64 TLSLPNILGAGGGAVATDD70 pKa = 5.3 AITGEE75 pKa = 3.47 IWFYY79 pKa = 10.36 TDD81 pKa = 4.77 GINVYY86 pKa = 10.76 DD87 pKa = 3.68 RR88 pKa = 11.84 TDD90 pKa = 3.53 FQLATTVDD98 pKa = 3.62 GSTTRR103 pKa = 11.84 NQSVAICEE111 pKa = 4.11 NPANSDD117 pKa = 3.4 QYY119 pKa = 12.0 FLFTINNAGAIVQSIFDD136 pKa = 3.73 KK137 pKa = 10.4 TQFRR141 pKa = 11.84 PGGAFPDD148 pKa = 3.92 SPEE151 pKa = 4.32 GNFTSLNNAVAPVTGPLSEE170 pKa = 4.13 GMIILSNDD178 pKa = 3.09 ALDD181 pKa = 4.38 GFWLITQTQGGDD193 pKa = 3.26 INVTEE198 pKa = 4.29 IDD200 pKa = 3.47 GTGTFNTTTFATTLTQIDD218 pKa = 4.21 NFSFHH223 pKa = 7.07 PSSNQLAAASAQAGEE238 pKa = 4.42 GVEE241 pKa = 5.68 LFDD244 pKa = 5.78 FDD246 pKa = 4.62 PATGAVTASATSLAAITFNGVYY268 pKa = 9.0 DD269 pKa = 4.19 TEE271 pKa = 4.62 WNNSGQYY278 pKa = 10.57 LYY280 pKa = 11.28 ASGNFGQPEE289 pKa = 4.05 DD290 pKa = 3.68 SLIRR294 pKa = 11.84 IDD296 pKa = 4.99 LNAVPPTVEE305 pKa = 4.21 NIQTFGVTNSFGIQIAPDD323 pKa = 3.25 STIYY327 pKa = 10.79 HH328 pKa = 6.87 LFEE331 pKa = 6.02 DD332 pKa = 3.95 PAGAFRR338 pKa = 11.84 VGRR341 pKa = 11.84 INDD344 pKa = 3.96 PDD346 pKa = 3.84 TSDD349 pKa = 3.43 VAQVLYY355 pKa = 10.5 DD356 pKa = 3.77 PRR358 pKa = 11.84 PFDD361 pKa = 3.76 VPDD364 pKa = 4.67 FQAEE368 pKa = 4.14 QFPSFLPLIDD378 pKa = 4.78 PDD380 pKa = 3.79 FTLDD384 pKa = 3.53 FSFFGDD390 pKa = 3.97 CQNEE394 pKa = 3.98 PVLFFPEE401 pKa = 4.07 ISINPDD407 pKa = 2.74 SVRR410 pKa = 11.84 WDD412 pKa = 3.5 FAGEE416 pKa = 4.11 GNSASLAPSFTFSDD430 pKa = 3.67 AAGSPFTVTLVAFINGQPRR449 pKa = 11.84 AASQQVTINPFDD461 pKa = 3.48 ISITLVSDD469 pKa = 3.32 TTFCRR474 pKa = 11.84 EE475 pKa = 4.13 DD476 pKa = 3.65 FPPPFGTNGTASVEE490 pKa = 4.11 AEE492 pKa = 3.69 ISGTPTSVVWSNGQTGNILVPDD514 pKa = 3.75 STGFYY519 pKa = 10.46 YY520 pKa = 10.83 VVATDD525 pKa = 3.6 ATGCSTYY532 pKa = 11.16 AGVQVNTYY540 pKa = 10.47 GEE542 pKa = 3.78 QDD544 pKa = 3.1 QRR546 pKa = 11.84 GNIWYY551 pKa = 9.16 FGNNAGIDD559 pKa = 4.07 FNPPPPTEE567 pKa = 4.98 AIPFGDD573 pKa = 3.18 ASIYY577 pKa = 10.94 NGGNQLRR584 pKa = 11.84 MPEE587 pKa = 4.01 GCAIYY592 pKa = 10.06 CDD594 pKa = 4.78 RR595 pKa = 11.84 NGQPLFYY602 pKa = 10.46 SDD604 pKa = 3.74 GVDD607 pKa = 3.55 VFDD610 pKa = 6.22 RR611 pKa = 11.84 EE612 pKa = 4.62 ANLLTPANKK621 pKa = 9.59 IGGTAGISQGSSQSVLVLPFPGDD644 pKa = 3.23 EE645 pKa = 4.01 TLFYY649 pKa = 11.08 LFTTNEE655 pKa = 4.0 VYY657 pKa = 10.74 NPSGSYY663 pKa = 8.19 EE664 pKa = 3.75 LRR666 pKa = 11.84 YY667 pKa = 10.3 SVFDD671 pKa = 3.94 LKK673 pKa = 11.31 LNNGLGGMVLDD684 pKa = 4.14 QNGEE688 pKa = 3.68 ISTILCSDD696 pKa = 3.21 VTEE699 pKa = 4.04 RR700 pKa = 11.84 LAGNEE705 pKa = 3.39 NWVIVHH711 pKa = 6.39 EE712 pKa = 4.5 LGNNNFRR719 pKa = 11.84 AYY721 pKa = 9.39 PVLGDD726 pKa = 4.21 GIGTPVISNIGAIHH740 pKa = 6.04 NSEE743 pKa = 4.23 LEE745 pKa = 3.87 SRR747 pKa = 11.84 GYY749 pKa = 9.65 MKK751 pKa = 11.07 LNGNSNQLAVAYY763 pKa = 10.59 SEE765 pKa = 4.27 STNNNFVEE773 pKa = 5.98 LFDD776 pKa = 4.64 FDD778 pKa = 3.97 NATGEE783 pKa = 4.24 VSNYY787 pKa = 10.01 LQLDD791 pKa = 3.87 FNNPVSSAGPVSGQVYY807 pKa = 10.37 GIEE810 pKa = 5.12 FGNNDD815 pKa = 3.31 NNLFASLIGTPSEE828 pKa = 3.72 IFLWHH833 pKa = 6.72 VDD835 pKa = 3.13 TTTIAGNITDD845 pKa = 3.66 PTYY848 pKa = 10.69 IRR850 pKa = 11.84 DD851 pKa = 3.66 SVEE854 pKa = 4.12 VVDD857 pKa = 6.22 LSSLPATDD865 pKa = 5.2 SIGALQLGPDD875 pKa = 3.56 GQMYY879 pKa = 10.2 GAIQNQQFTVTIGNTGTRR897 pKa = 11.84 FGQPVPVANTFGLAAGTSSQLGLPNFIQFSGSSSQNASLAVEE939 pKa = 4.82 SGCIDD944 pKa = 3.31 QPLTFTVNNPLNPNLEE960 pKa = 4.15 IYY962 pKa = 9.97 FVSIFPEE969 pKa = 4.4 GDD971 pKa = 3.07 EE972 pKa = 4.68 SNVLVGPIQLTQDD985 pKa = 3.35 NPDD988 pKa = 3.45 FTFSLPDD995 pKa = 3.75 PGNYY999 pKa = 8.74 VARR1002 pKa = 11.84 FFIDD1006 pKa = 3.34 QQCIPQQLITDD1017 pKa = 4.14 SDD1019 pKa = 3.71 QLFAINPLPTVSNIAVTDD1037 pKa = 3.69 PTGCGLPDD1045 pKa = 3.83 GSAIVTFSTTGNITYY1060 pKa = 10.07 SVSGPVSYY1068 pKa = 10.57 PDD1070 pKa = 3.18 QTVTTTGSTNVTIPALSAGFYY1091 pKa = 9.42 TLNAAFDD1098 pKa = 4.12 TGCSEE1103 pKa = 4.21 TFTFILNDD1111 pKa = 3.66 PVPYY1115 pKa = 9.86 TLAAVQSQGSDD1126 pKa = 3.21 CDD1128 pKa = 3.89 DD1129 pKa = 3.44 QNGEE1133 pKa = 4.03 ITVTLSPLAQVPAAYY1148 pKa = 9.96 DD1149 pKa = 3.24 WVLNEE1154 pKa = 4.02 QSTGTTVRR1162 pKa = 11.84 TGNQADD1168 pKa = 4.51 DD1169 pKa = 4.39 PDD1171 pKa = 4.71 SDD1173 pKa = 4.12 GVFVITGVSTGNYY1186 pKa = 9.49 FLEE1189 pKa = 4.53 LTDD1192 pKa = 3.63 NAGCISTVNVSITPPPSITLTLPDD1216 pKa = 4.44 QFFQACDD1223 pKa = 3.1 VDD1225 pKa = 4.96 EE1226 pKa = 4.31 IVIPITTNSNSLVEE1240 pKa = 4.77 LLSQTGDD1247 pKa = 3.19 LLTNFRR1253 pKa = 11.84 VIGQNDD1259 pKa = 4.15 SIAITNPGISGEE1271 pKa = 4.09 PIIFGIRR1278 pKa = 11.84 ALGEE1282 pKa = 4.21 DD1283 pKa = 5.1 PISCDD1288 pKa = 3.09 TTVVIQVIFDD1298 pKa = 3.62 NSSPNPFDD1306 pKa = 4.31 SRR1308 pKa = 11.84 YY1309 pKa = 7.39 TLCPFEE1315 pKa = 4.76 SFEE1318 pKa = 4.29 SGRR1321 pKa = 11.84 QVVMLEE1327 pKa = 4.08 NPPSGFQSARR1337 pKa = 11.84 WFDD1340 pKa = 3.87 RR1341 pKa = 11.84 NSVEE1345 pKa = 4.29 IEE1347 pKa = 4.09 RR1348 pKa = 11.84 INASGASNVTGYY1360 pKa = 10.62 RR1361 pKa = 11.84 FSQAGDD1367 pKa = 3.68 SIIIEE1372 pKa = 3.99 IAQPISVEE1380 pKa = 3.82 LTNAFGCPTVASIDD1394 pKa = 3.39 IVQDD1398 pKa = 3.19 CKK1400 pKa = 11.31 GRR1402 pKa = 11.84 INAPSAFYY1410 pKa = 10.22 PNSLIPRR1417 pKa = 11.84 NRR1419 pKa = 11.84 QFVVFPFLILPDD1431 pKa = 4.31 DD1432 pKa = 3.73 FQIYY1436 pKa = 9.96 IFNRR1440 pKa = 11.84 WGEE1443 pKa = 4.14 MVFQSDD1449 pKa = 3.26 NLQFMRR1455 pKa = 11.84 EE1456 pKa = 4.01 QGWNGGYY1463 pKa = 10.7 DD1464 pKa = 3.84 NDD1466 pKa = 4.66 PGRR1469 pKa = 11.84 PVPGGGYY1476 pKa = 10.37 AYY1478 pKa = 10.23 RR1479 pKa = 11.84 INYY1482 pKa = 8.73 KK1483 pKa = 9.78 SQFEE1487 pKa = 3.92 PDD1489 pKa = 3.16 EE1490 pKa = 4.71 LKK1492 pKa = 10.47 EE1493 pKa = 3.95 QRR1495 pKa = 11.84 GGVTLIRR1502 pKa = 4.51
Molecular weight: 161.61 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.724
IPC2_protein 3.719
IPC_protein 3.77
Toseland 3.541
ProMoST 3.923
Dawson 3.757
Bjellqvist 3.91
Wikipedia 3.694
Rodwell 3.592
Grimsley 3.439
Solomon 3.757
Lehninger 3.719
Nozaki 3.859
DTASelect 4.126
Thurlkill 3.579
EMBOSS 3.694
Sillero 3.884
Patrickios 0.68
IPC_peptide 3.757
IPC2_peptide 3.872
IPC2.peptide.svr19 3.801
Protein with the highest isoelectric point:
>tr|A0A553FN91|A0A553FN91_9BACT Uncharacterized protein OS=Fulvivirga sp. M361 OX=2594266 GN=FNH22_12595 PE=4 SV=1
MM1 pKa = 7.88 SSFTPWRR8 pKa = 11.84 AYY10 pKa = 10.51 SDD12 pKa = 3.26 SAMRR16 pKa = 11.84 ADD18 pKa = 3.99 LGIGFVIRR26 pKa = 11.84 VYY28 pKa = 10.76 FRR30 pKa = 11.84 GSFRR34 pKa = 11.84 GMIRR38 pKa = 11.84 QCFIIKK44 pKa = 8.93 TIQNKK49 pKa = 9.58 FIKK52 pKa = 10.36 NMRR55 pKa = 11.84 ITNAKK60 pKa = 10.1 CSILKK65 pKa = 10.39 EE66 pKa = 3.97 NGNALRR72 pKa = 11.84 FAKK75 pKa = 9.86 YY76 pKa = 10.2 APEE79 pKa = 4.58 GDD81 pKa = 3.06 IRR83 pKa = 11.84 NIHH86 pKa = 7.02 LSPQIEE92 pKa = 3.89 NAAVNEE98 pKa = 3.86 ARR100 pKa = 11.84 ADD102 pKa = 3.57 ALSRR106 pKa = 11.84 LL107 pKa = 4.04
Molecular weight: 12.17 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.318
IPC2_protein 9.56
IPC_protein 10.072
Toseland 10.467
ProMoST 10.116
Dawson 10.599
Bjellqvist 10.277
Wikipedia 10.774
Rodwell 10.891
Grimsley 10.643
Solomon 10.672
Lehninger 10.643
Nozaki 10.467
DTASelect 10.262
Thurlkill 10.482
EMBOSS 10.862
Sillero 10.526
Patrickios 10.657
IPC_peptide 10.672
IPC2_peptide 9.253
IPC2.peptide.svr19 8.554
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
6272
0
6272
2376036
24
4706
378.8
42.67
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
6.39 ± 0.025
0.736 ± 0.011
5.925 ± 0.024
6.539 ± 0.03
5.023 ± 0.022
6.892 ± 0.031
1.955 ± 0.016
7.119 ± 0.024
6.366 ± 0.039
9.44 ± 0.034
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.194 ± 0.015
5.504 ± 0.027
3.72 ± 0.019
3.567 ± 0.016
4.206 ± 0.019
6.831 ± 0.024
5.793 ± 0.034
6.451 ± 0.024
1.271 ± 0.013
4.079 ± 0.023
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here