Tortoise microvirus 16

Taxonomy: Viruses; Monodnaviria; Sangervirae; Phixviricota; Malgrandaviricetes; Petitvirales; Microviridae; unclassified Microviridae

Average proteome isoelectric point is 5.9

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 8 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A4P8W6V9|A0A4P8W6V9_9VIRU Uncharacterized protein OS=Tortoise microvirus 16 OX=2583117 PE=4 SV=1
MM1 pKa = 6.96TASSVDD7 pKa = 3.88SIMTCLDD14 pKa = 2.92RR15 pKa = 11.84CYY17 pKa = 10.87LLDD20 pKa = 3.88KK21 pKa = 11.04LSVLRR26 pKa = 11.84PLPNQRR32 pKa = 11.84FCCDD36 pKa = 2.83SYY38 pKa = 11.59DD39 pKa = 3.82YY40 pKa = 11.37KK41 pKa = 10.96GAHH44 pKa = 6.21YY45 pKa = 10.28DD46 pKa = 3.42YY47 pKa = 10.16ATFRR51 pKa = 11.84IVRR54 pKa = 11.84RR55 pKa = 11.84NFTGKK60 pKa = 10.44LEE62 pKa = 4.11YY63 pKa = 10.41AVLVEE68 pKa = 3.75PCGIFKK74 pKa = 10.55HH75 pKa = 6.87DD76 pKa = 4.19DD77 pKa = 3.09SHH79 pKa = 8.2YY80 pKa = 10.3LTFDD84 pKa = 3.51EE85 pKa = 5.07YY86 pKa = 11.56DD87 pKa = 3.7SFCLSVSEE95 pKa = 5.44DD96 pKa = 4.19DD97 pKa = 3.54II98 pKa = 6.75

Molecular weight: 11.42 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A4P8W658|A0A4P8W658_9VIRU DNA pilot protein OS=Tortoise microvirus 16 OX=2583117 PE=4 SV=1
MM1 pKa = 6.87MFSMRR6 pKa = 11.84SIAPSTRR13 pKa = 11.84FVGALRR19 pKa = 11.84DD20 pKa = 3.85TRR22 pKa = 11.84SMIVGSFDD30 pKa = 3.8CLHH33 pKa = 6.2FTAKK37 pKa = 8.88TLSRR41 pKa = 11.84ILFAPRR47 pKa = 11.84SWRR50 pKa = 11.84CIMLIAQTVLFYY62 pKa = 10.46QVAMLVVLISPFYY75 pKa = 8.82FLKK78 pKa = 10.67RR79 pKa = 11.84FFRR82 pKa = 11.84RR83 pKa = 11.84LTIYY87 pKa = 10.82

Molecular weight: 10.25 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

8

0

8

1747

83

580

218.4

24.7

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.098 ± 1.08

1.717 ± 0.64

6.754 ± 0.821

4.35 ± 1.008

5.896 ± 0.977

5.381 ± 0.544

1.546 ± 0.351

4.808 ± 0.608

3.95 ± 0.91

9.33 ± 0.561

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.919 ± 0.476

4.179 ± 0.617

4.751 ± 0.574

4.007 ± 1.398

6.239 ± 0.596

10.246 ± 0.973

5.953 ± 0.947

4.808 ± 0.739

0.916 ± 0.211

5.152 ± 0.732
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski