Rhodobacteraceae bacterium HIMB11

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Alphaproteobacteria; Rhodobacterales; Rhodobacteraceae; unclassified Rhodobacteraceae

Average proteome isoelectric point is 5.99

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3183 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|U4V326|U4V326_9RHOB Uncharacterized protein OS=Rhodobacteraceae bacterium HIMB11 OX=1366046 GN=HIMB11_00006 PE=4 SV=1
MM1 pKa = 7.48KK2 pKa = 10.29KK3 pKa = 10.36VLLTTTALVVTAGVAAAEE21 pKa = 4.23VSMSGKK27 pKa = 10.04AQVSAGATGNADD39 pKa = 3.35MALEE43 pKa = 4.07THH45 pKa = 6.96LDD47 pKa = 3.73LNLSMSMTADD57 pKa = 2.93NGMTMSTAIGYY68 pKa = 9.89DD69 pKa = 3.18SGNMVDD75 pKa = 3.82GDD77 pKa = 4.39HH78 pKa = 7.52YY79 pKa = 11.64DD80 pKa = 3.52GDD82 pKa = 4.41EE83 pKa = 4.26NNVWSAGAAPSLTIGYY99 pKa = 8.86EE100 pKa = 4.43GYY102 pKa = 9.27TITADD107 pKa = 3.5STGVADD113 pKa = 6.09LYY115 pKa = 11.6NGDD118 pKa = 4.34KK119 pKa = 11.18NSGNLGVAGSMGDD132 pKa = 3.18VSFALTGNIGGDD144 pKa = 3.66DD145 pKa = 3.55TTVAGGSSYY154 pKa = 11.57SLGYY158 pKa = 9.48TMGDD162 pKa = 3.03MTIAIVGSDD171 pKa = 3.4SADD174 pKa = 3.12GVINTDD180 pKa = 2.93AMKK183 pKa = 9.82ATVTYY188 pKa = 11.11KK189 pKa = 10.3MGDD192 pKa = 3.41TTITASSDD200 pKa = 3.38DD201 pKa = 3.74KK202 pKa = 11.75DD203 pKa = 3.83GVEE206 pKa = 4.19TVTAIGFSTVMDD218 pKa = 5.54SITVAYY224 pKa = 6.93TAKK227 pKa = 9.13NTEE230 pKa = 4.21TAGSSVGDD238 pKa = 3.32HH239 pKa = 6.7WDD241 pKa = 2.98ASIAYY246 pKa = 9.49SAGALTASFALDD258 pKa = 3.52EE259 pKa = 4.87ADD261 pKa = 4.97KK262 pKa = 10.47STLKK266 pKa = 10.7AGYY269 pKa = 9.59DD270 pKa = 3.51LGGGADD276 pKa = 4.23LFAVMVNGAAANGSEE291 pKa = 4.37DD292 pKa = 3.55FQAIGINFAFF302 pKa = 4.68

Molecular weight: 30.27 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|U4UXT2|U4UXT2_9RHOB DUF1330 domain-containing protein OS=Rhodobacteraceae bacterium HIMB11 OX=1366046 GN=HIMB11_02318 PE=4 SV=1
MM1 pKa = 7.81GIIDD5 pKa = 4.55KK6 pKa = 10.76FDD8 pKa = 4.06DD9 pKa = 3.97SVRR12 pKa = 11.84LNLRR16 pKa = 11.84RR17 pKa = 11.84FGLTALQLFSVTVALNVPLEE37 pKa = 4.0VALLCGTSRR46 pKa = 11.84SALALLGSRR55 pKa = 11.84PAKK58 pKa = 9.88IRR60 pKa = 11.84PTVGRR65 pKa = 11.84QMYY68 pKa = 8.0RR69 pKa = 11.84TAKK72 pKa = 10.05HH73 pKa = 5.74LLANFSPFAEE83 pKa = 4.17SSTIKK88 pKa = 10.36PCLLFAMQLDD98 pKa = 4.35GLDD101 pKa = 3.01AWRR104 pKa = 11.84TRR106 pKa = 11.84FGRR109 pKa = 11.84EE110 pKa = 4.0ATWTLLQHH118 pKa = 6.81AYY120 pKa = 10.73DD121 pKa = 5.48RR122 pKa = 11.84INCASTGLRR131 pKa = 11.84KK132 pKa = 9.69GVAWMEE138 pKa = 4.59GYY140 pKa = 9.8FKK142 pKa = 10.76KK143 pKa = 10.21QRR145 pKa = 11.84RR146 pKa = 11.84KK147 pKa = 10.04AAVV150 pKa = 2.92

Molecular weight: 16.82 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3183

0

3183

933435

17

1511

293.3

32.18

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

10.567 ± 0.05

1.027 ± 0.014

6.221 ± 0.034

5.827 ± 0.044

4.07 ± 0.031

7.987 ± 0.039

2.294 ± 0.023

6.215 ± 0.035

4.103 ± 0.033

9.412 ± 0.047

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

3.014 ± 0.023

3.451 ± 0.026

4.476 ± 0.024

3.52 ± 0.026

5.619 ± 0.036

5.717 ± 0.03

5.506 ± 0.027

7.039 ± 0.034

1.357 ± 0.018

2.576 ± 0.021
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski