Amino acid dipepetide frequency for Rhodobacteraceae bacterium HIMB11

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10^-3.

For more information see sequence space article on Wikipedia.

	Ala	Cys	Asp	Glu	Phe	Gly	His	Ile	Lys	Leu	Met	Asn	Pro	Gln	Arg	Ser	Thr	Val	Trp	Tyr	Xaa
Ala	12.082AlaAla: 12.082 ± 0.151	1.076AlaCys: 1.076 ± 0.036	6.318AlaAsp: 6.318 ± 0.104	6.651AlaGlu: 6.651 ± 0.111	4.017AlaPhe: 4.017 ± 0.075	8.389AlaGly: 8.389 ± 0.097	2.377AlaHis: 2.377 ± 0.05	6.406AlaIle: 6.406 ± 0.08	4.632AlaLys: 4.632 ± 0.089	10.877AlaLeu: 10.877 ± 0.136	3.482AlaMet: 3.482 ± 0.073	3.285AlaAsn: 3.285 ± 0.063	4.385AlaPro: 4.385 ± 0.069	4.249AlaGln: 4.249 ± 0.072	6.021AlaArg: 6.021 ± 0.083	5.387AlaSer: 5.387 ± 0.093	5.395AlaThr: 5.395 ± 0.081	6.792AlaVal: 6.792 ± 0.104	1.187AlaTrp: 1.187 ± 0.036	2.666AlaTyr: 2.666 ± 0.056	0.0AlaXaa: 0.0 ± 0.0
Cys	1.116CysAla: 1.116 ± 0.03	0.147CysCys: 0.147 ± 0.013	0.784CysAsp: 0.784 ± 0.028	0.549CysGlu: 0.549 ± 0.023	0.412CysPhe: 0.412 ± 0.022	1.046CysGly: 1.046 ± 0.039	0.305CysHis: 0.305 ± 0.021	0.55CysIle: 0.55 ± 0.024	0.309CysLys: 0.309 ± 0.017	0.868CysLeu: 0.868 ± 0.031	0.247CysMet: 0.247 ± 0.014	0.312CysAsn: 0.312 ± 0.02	0.524CysPro: 0.524 ± 0.025	0.318CysGln: 0.318 ± 0.018	0.494CysArg: 0.494 ± 0.02	0.624CysSer: 0.624 ± 0.029	0.483CysThr: 0.483 ± 0.019	0.741CysVal: 0.741 ± 0.03	0.135CysTrp: 0.135 ± 0.012	0.307CysTyr: 0.307 ± 0.018	0.0CysXaa: 0.0 ± 0.0
Asp	7.035AspAla: 7.035 ± 0.107	0.537AspCys: 0.537 ± 0.027	3.512AspAsp: 3.512 ± 0.082	3.691AspGlu: 3.691 ± 0.077	2.534AspPhe: 2.534 ± 0.053	4.978AspGly: 4.978 ± 0.08	1.67AspHis: 1.67 ± 0.05	3.92AspIle: 3.92 ± 0.062	2.115AspLys: 2.115 ± 0.044	6.248AspLeu: 6.248 ± 0.081	2.027AspMet: 2.027 ± 0.045	1.621AspAsn: 1.621 ± 0.038	3.364AspPro: 3.364 ± 0.064	2.643AspGln: 2.643 ± 0.064	3.652AspArg: 3.652 ± 0.056	2.005AspSer: 2.005 ± 0.049	3.088AspThr: 3.088 ± 0.061	4.744AspVal: 4.744 ± 0.08	1.123AspTrp: 1.123 ± 0.037	1.703AspTyr: 1.703 ± 0.042	0.0AspXaa: 0.0 ± 0.0
Glu	6.244GluAla: 6.244 ± 0.107	0.509GluCys: 0.509 ± 0.024	3.099GluAsp: 3.099 ± 0.061	3.366GluGlu: 3.366 ± 0.086	2.314GluPhe: 2.314 ± 0.054	3.914GluGly: 3.914 ± 0.07	1.437GluHis: 1.437 ± 0.039	4.135GluIle: 4.135 ± 0.077	2.771GluLys: 2.771 ± 0.062	5.296GluLeu: 5.296 ± 0.078	1.834GluMet: 1.834 ± 0.044	2.578GluAsn: 2.578 ± 0.05	2.076GluPro: 2.076 ± 0.051	2.377GluGln: 2.377 ± 0.047	4.024GluArg: 4.024 ± 0.071	2.083GluSer: 2.083 ± 0.05	3.995GluThr: 3.995 ± 0.07	3.945GluVal: 3.945 ± 0.08	0.809GluTrp: 0.809 ± 0.026	1.468GluTyr: 1.468 ± 0.044	0.0GluXaa: 0.0 ± 0.0
Phe	4.24PheAla: 4.24 ± 0.081	0.496PheCys: 0.496 ± 0.021	3.19PheAsp: 3.19 ± 0.057	2.549PheGlu: 2.549 ± 0.051	1.708PhePhe: 1.708 ± 0.051	3.813PheGly: 3.813 ± 0.076	0.867PheHis: 0.867 ± 0.032	2.17PheIle: 2.17 ± 0.055	1.344PheLys: 1.344 ± 0.043	3.481PheLeu: 3.481 ± 0.079	1.054PheMet: 1.054 ± 0.039	1.392PheAsn: 1.392 ± 0.039	1.613PhePro: 1.613 ± 0.044	1.316PheGln: 1.316 ± 0.045	1.884PheArg: 1.884 ± 0.043	2.595PheSer: 2.595 ± 0.055	2.205PheThr: 2.205 ± 0.048	3.034PheVal: 3.034 ± 0.065	0.606PheTrp: 0.606 ± 0.029	1.141PheTyr: 1.141 ± 0.037	0.0PheXaa: 0.0 ± 0.0
Gly	8.403GlyAla: 8.403 ± 0.096	0.926GlyCys: 0.926 ± 0.033	4.473GlyAsp: 4.473 ± 0.079	4.27GlyGlu: 4.27 ± 0.065	3.563GlyPhe: 3.563 ± 0.066	6.334GlyGly: 6.334 ± 0.103	1.935GlyHis: 1.935 ± 0.05	4.899GlyIle: 4.899 ± 0.074	3.408GlyLys: 3.408 ± 0.067	7.85GlyLeu: 7.85 ± 0.099	2.593GlyMet: 2.593 ± 0.061	2.301GlyAsn: 2.301 ± 0.043	3.016GlyPro: 3.016 ± 0.059	3.099GlyGln: 3.099 ± 0.06	4.445GlyArg: 4.445 ± 0.068	4.371GlySer: 4.371 ± 0.072	4.235GlyThr: 4.235 ± 0.074	6.008GlyVal: 6.008 ± 0.089	1.216GlyTrp: 1.216 ± 0.035	2.528GlyTyr: 2.528 ± 0.055	0.0GlyXaa: 0.0 ± 0.0
His	2.425HisAla: 2.425 ± 0.048	0.283HisCys: 0.283 ± 0.017	1.406HisAsp: 1.406 ± 0.04	1.25HisGlu: 1.25 ± 0.034	0.972HisPhe: 0.972 ± 0.032	1.987HisGly: 1.987 ± 0.049	0.665HisHis: 0.665 ± 0.028	1.319HisIle: 1.319 ± 0.037	0.792HisLys: 0.792 ± 0.032	2.223HisLeu: 2.223 ± 0.043	0.733HisMet: 0.733 ± 0.029	0.675HisAsn: 0.675 ± 0.026	1.418HisPro: 1.418 ± 0.037	0.738HisGln: 0.738 ± 0.03	1.229HisArg: 1.229 ± 0.039	1.176HisSer: 1.176 ± 0.039	0.986HisThr: 0.986 ± 0.034	1.623HisVal: 1.623 ± 0.045	0.381HisTrp: 0.381 ± 0.023	0.659HisTyr: 0.659 ± 0.025	0.0HisXaa: 0.0 ± 0.0
Ile	7.258IleAla: 7.258 ± 0.102	0.806IleCys: 0.806 ± 0.029	4.182IleAsp: 4.182 ± 0.067	4.177IleGlu: 4.177 ± 0.075	2.329IlePhe: 2.329 ± 0.055	5.32IleGly: 5.32 ± 0.089	1.136IleHis: 1.136 ± 0.036	3.319IleIle: 3.319 ± 0.067	2.451IleLys: 2.451 ± 0.056	5.82IleLeu: 5.82 ± 0.081	1.499IleMet: 1.499 ± 0.039	2.087IleAsn: 2.087 ± 0.052	2.656IlePro: 2.656 ± 0.055	1.818IleGln: 1.818 ± 0.039	2.993IleArg: 2.993 ± 0.048	3.994IleSer: 3.994 ± 0.059	3.629IleThr: 3.629 ± 0.065	4.354IleVal: 4.354 ± 0.072	0.84IleTrp: 0.84 ± 0.029	1.485IleTyr: 1.485 ± 0.037	0.0IleXaa: 0.0 ± 0.0
Lys	3.858LysAla: 3.858 ± 0.069	0.318LysCys: 0.318 ± 0.018	2.288LysAsp: 2.288 ± 0.049	2.151LysGlu: 2.151 ± 0.053	1.533LysPhe: 1.533 ± 0.046	2.855LysGly: 2.855 ± 0.056	0.921LysHis: 0.921 ± 0.03	2.676LysIle: 2.676 ± 0.058	1.907LysLys: 1.907 ± 0.045	3.927LysLeu: 3.927 ± 0.072	1.246LysMet: 1.246 ± 0.042	1.617LysAsn: 1.617 ± 0.042	2.0LysPro: 2.0 ± 0.051	1.411LysGln: 1.411 ± 0.039	2.643LysArg: 2.643 ± 0.053	2.897LysSer: 2.897 ± 0.061	2.666LysThr: 2.666 ± 0.051	2.57LysVal: 2.57 ± 0.056	0.517LysTrp: 0.517 ± 0.024	1.023LysTyr: 1.023 ± 0.033	0.0LysXaa: 0.0 ± 0.0
Leu	9.516LeuAla: 9.516 ± 0.113	1.088LeuCys: 1.088 ± 0.034	5.863LeuAsp: 5.863 ± 0.085	5.129LeuGlu: 5.129 ± 0.084	3.708LeuPhe: 3.708 ± 0.076	7.9LeuGly: 7.9 ± 0.09	1.952LeuHis: 1.952 ± 0.044	6.154LeuIle: 6.154 ± 0.09	3.833LeuLys: 3.833 ± 0.067	7.723LeuLeu: 7.723 ± 0.117	2.789LeuMet: 2.789 ± 0.057	3.686LeuAsn: 3.686 ± 0.062	4.612LeuPro: 4.612 ± 0.07	2.87LeuGln: 2.87 ± 0.062	5.812LeuArg: 5.812 ± 0.092	7.009LeuSer: 7.009 ± 0.107	5.349LeuThr: 5.349 ± 0.074	5.86LeuVal: 5.86 ± 0.092	1.168LeuTrp: 1.168 ± 0.037	2.101LeuTyr: 2.101 ± 0.047	0.0LeuXaa: 0.0 ± 0.0
Met	3.179MetAla: 3.179 ± 0.053	0.296MetCys: 0.296 ± 0.018	1.739MetAsp: 1.739 ± 0.043	1.406MetGlu: 1.406 ± 0.04	0.979MetPhe: 0.979 ± 0.035	2.436MetGly: 2.436 ± 0.057	0.641MetHis: 0.641 ± 0.025	1.934MetIle: 1.934 ± 0.051	1.38MetLys: 1.38 ± 0.037	2.512MetLeu: 2.512 ± 0.048	0.991MetMet: 0.991 ± 0.036	1.14MetAsn: 1.14 ± 0.035	1.504MetPro: 1.504 ± 0.044	1.196MetGln: 1.196 ± 0.035	1.943MetArg: 1.943 ± 0.048	2.116MetSer: 2.116 ± 0.05	2.048MetThr: 2.048 ± 0.043	1.943MetVal: 1.943 ± 0.039	0.284MetTrp: 0.284 ± 0.015	0.479MetTyr: 0.479 ± 0.023	0.0MetXaa: 0.0 ± 0.0
Asn	3.93AsnAla: 3.93 ± 0.07	0.405AsnCys: 0.405 ± 0.019	2.046AsnAsp: 2.046 ± 0.058	1.758AsnGlu: 1.758 ± 0.04	1.432AsnPhe: 1.432 ± 0.035	2.905AsnGly: 2.905 ± 0.064	0.71AsnHis: 0.71 ± 0.028	2.187AsnIle: 2.187 ± 0.05	1.177AsnLys: 1.177 ± 0.041	3.245AsnLeu: 3.245 ± 0.062	0.982AsnMet: 0.982 ± 0.03	1.096AsnAsn: 1.096 ± 0.04	2.118AsnPro: 2.118 ± 0.046	1.064AsnGln: 1.064 ± 0.035	1.861AsnArg: 1.861 ± 0.047	1.807AsnSer: 1.807 ± 0.047	1.818AsnThr: 1.818 ± 0.042	2.464AsnVal: 2.464 ± 0.052	0.58AsnTrp: 0.58 ± 0.024	0.929AsnTyr: 0.929 ± 0.033	0.0AsnXaa: 0.0 ± 0.0
Pro	4.149ProAla: 4.149 ± 0.081	0.364ProCys: 0.364 ± 0.019	3.365ProAsp: 3.365 ± 0.062	3.419ProGlu: 3.419 ± 0.067	1.959ProPhe: 1.959 ± 0.047	2.781ProGly: 2.781 ± 0.053	1.054ProHis: 1.054 ± 0.031	2.89ProIle: 2.89 ± 0.058	2.337ProLys: 2.337 ± 0.051	3.844ProLeu: 3.844 ± 0.077	1.362ProMet: 1.362 ± 0.041	1.934ProAsn: 1.934 ± 0.048	1.509ProPro: 1.509 ± 0.048	1.386ProGln: 1.386 ± 0.035	1.941ProArg: 1.941 ± 0.048	2.692ProSer: 2.692 ± 0.056	2.578ProThr: 2.578 ± 0.053	3.384ProVal: 3.384 ± 0.065	0.597ProTrp: 0.597 ± 0.026	1.215ProTyr: 1.215 ± 0.036	0.0ProXaa: 0.0 ± 0.0
Gln	3.722GlnAla: 3.722 ± 0.065	0.257GlnCys: 0.257 ± 0.017	2.082GlnAsp: 2.082 ± 0.049	1.838GlnGlu: 1.838 ± 0.045	1.424GlnPhe: 1.424 ± 0.042	2.685GlnGly: 2.685 ± 0.049	0.787GlnHis: 0.787 ± 0.028	2.498GlnIle: 2.498 ± 0.051	1.584GlnLys: 1.584 ± 0.042	2.985GlnLeu: 2.985 ± 0.061	1.251GlnMet: 1.251 ± 0.036	1.512GlnAsn: 1.512 ± 0.043	1.29GlnPro: 1.29 ± 0.036	1.163GlnGln: 1.163 ± 0.042	2.108GlnArg: 2.108 ± 0.05	2.357GlnSer: 2.357 ± 0.052	2.033GlnThr: 2.033 ± 0.049	2.419GlnVal: 2.419 ± 0.051	0.433GlnTrp: 0.433 ± 0.02	0.772GlnTyr: 0.772 ± 0.028	0.0GlnXaa: 0.0 ± 0.0
Arg	6.245ArgAla: 6.245 ± 0.1	0.44ArgCys: 0.44 ± 0.024	3.687ArgAsp: 3.687 ± 0.072	3.248ArgGlu: 3.248 ± 0.072	2.32ArgPhe: 2.32 ± 0.05	3.803ArgGly: 3.803 ± 0.068	1.369ArgHis: 1.369 ± 0.04	3.562ArgIle: 3.562 ± 0.059	2.33ArgLys: 2.33 ± 0.057	5.612ArgLeu: 5.612 ± 0.084	1.731ArgMet: 1.731 ± 0.043	1.838ArgAsn: 1.838 ± 0.043	2.449ArgPro: 2.449 ± 0.054	2.022ArgGln: 2.022 ± 0.049	3.454ArgArg: 3.454 ± 0.062	2.979ArgSer: 2.979 ± 0.059	2.634ArgThr: 2.634 ± 0.054	4.206ArgVal: 4.206 ± 0.066	0.727ArgTrp: 0.727 ± 0.027	1.536ArgTyr: 1.536 ± 0.037	0.0ArgXaa: 0.0 ± 0.0
Ser	5.97SerAla: 5.97 ± 0.072	0.532SerCys: 0.532 ± 0.022	3.862SerAsp: 3.862 ± 0.066	3.527SerGlu: 3.527 ± 0.06	2.552SerPhe: 2.552 ± 0.058	5.38SerGly: 5.38 ± 0.087	1.234SerHis: 1.234 ± 0.034	3.376SerIle: 3.376 ± 0.064	2.483SerLys: 2.483 ± 0.057	5.118SerLeu: 5.118 ± 0.075	1.684SerMet: 1.684 ± 0.043	2.011SerAsn: 2.011 ± 0.045	2.23SerPro: 2.23 ± 0.047	1.88SerGln: 1.88 ± 0.043	2.845SerArg: 2.845 ± 0.051	3.086SerSer: 3.086 ± 0.066	2.833SerThr: 2.833 ± 0.06	4.293SerVal: 4.293 ± 0.07	0.72SerTrp: 0.72 ± 0.029	1.548SerTyr: 1.548 ± 0.038	0.0SerXaa: 0.0 ± 0.0
Thr	5.337ThrAla: 5.337 ± 0.087	0.585ThrCys: 0.585 ± 0.028	3.137ThrAsp: 3.137 ± 0.052	2.835ThrGlu: 2.835 ± 0.055	2.185ThrPhe: 2.185 ± 0.045	4.863ThrGly: 4.863 ± 0.074	1.369ThrHis: 1.369 ± 0.038	3.288ThrIle: 3.288 ± 0.065	2.116ThrLys: 2.116 ± 0.055	5.904ThrLeu: 5.904 ± 0.086	1.401ThrMet: 1.401 ± 0.038	1.772ThrAsn: 1.772 ± 0.04	3.19ThrPro: 3.19 ± 0.055	1.966ThrGln: 1.966 ± 0.044	2.904ThrArg: 2.904 ± 0.055	3.1ThrSer: 3.1 ± 0.056	3.001ThrThr: 3.001 ± 0.062	3.914ThrVal: 3.914 ± 0.064	0.71ThrTrp: 0.71 ± 0.028	1.484ThrTyr: 1.484 ± 0.042	0.0ThrXaa: 0.0 ± 0.0
Val	7.134ValAla: 7.134 ± 0.102	0.717ValCys: 0.717 ± 0.029	4.44ValAsp: 4.44 ± 0.07	4.295ValGlu: 4.295 ± 0.071	2.995ValPhe: 2.995 ± 0.057	5.257ValGly: 5.257 ± 0.076	1.484ValHis: 1.484 ± 0.042	4.796ValIle: 4.796 ± 0.076	2.711ValLys: 2.711 ± 0.056	6.661ValLeu: 6.661 ± 0.084	2.177ValMet: 2.177 ± 0.043	2.315ValAsn: 2.315 ± 0.052	3.074ValPro: 3.074 ± 0.058	2.228ValGln: 2.228 ± 0.057	3.555ValArg: 3.555 ± 0.07	4.491ValSer: 4.491 ± 0.069	4.173ValThr: 4.173 ± 0.057	5.366ValVal: 5.366 ± 0.088	0.884ValTrp: 0.884 ± 0.03	1.637ValTyr: 1.637 ± 0.046	0.0ValXaa: 0.0 ± 0.0
Trp	1.23TrpAla: 1.23 ± 0.038	0.157TrpCys: 0.157 ± 0.013	0.874TrpAsp: 0.874 ± 0.029	0.655TrpGlu: 0.655 ± 0.026	0.571TrpPhe: 0.571 ± 0.027	1.039TrpGly: 1.039 ± 0.038	0.34TrpHis: 0.34 ± 0.019	0.811TrpIle: 0.811 ± 0.028	0.516TrpLys: 0.516 ± 0.024	1.412TrpLeu: 1.412 ± 0.049	0.451TrpMet: 0.451 ± 0.024	0.515TrpAsn: 0.515 ± 0.025	0.598TrpPro: 0.598 ± 0.028	0.53TrpGln: 0.53 ± 0.026	0.915TrpArg: 0.915 ± 0.028	0.868TrpSer: 0.868 ± 0.033	0.662TrpThr: 0.662 ± 0.028	0.893TrpVal: 0.893 ± 0.035	0.219TrpTrp: 0.219 ± 0.017	0.312TrpTyr: 0.312 ± 0.02	0.0TrpXaa: 0.0 ± 0.0
Tyr	2.6TyrAla: 2.6 ± 0.054	0.332TyrCys: 0.332 ± 0.019	1.862TyrAsp: 1.862 ± 0.047	1.509TyrGlu: 1.509 ± 0.041	1.189TyrPhe: 1.189 ± 0.035	2.187TyrGly: 2.187 ± 0.05	0.687TyrHis: 0.687 ± 0.026	1.262TyrIle: 1.262 ± 0.033	0.828TyrLys: 0.828 ± 0.03	2.518TyrLeu: 2.518 ± 0.044	0.61TyrMet: 0.61 ± 0.025	0.829TyrAsn: 0.829 ± 0.034	1.133TyrPro: 1.133 ± 0.032	0.927TyrGln: 0.927 ± 0.032	1.471TyrArg: 1.471 ± 0.045	1.53TyrSer: 1.53 ± 0.041	1.251TyrThr: 1.251 ± 0.038	1.836TyrVal: 1.836 ± 0.047	0.433TyrTrp: 0.433 ± 0.024	0.77TyrTyr: 0.77 ± 0.03	0.0TyrXaa: 0.0 ± 0.0
Xaa	0.0XaaAla: 0.0 ± 0.0	0.0XaaCys: 0.0 ± 0.0	0.0XaaAsp: 0.0 ± 0.0	0.0XaaGlu: 0.0 ± 0.0	0.0XaaPhe: 0.0 ± 0.0	0.0XaaGly: 0.0 ± 0.0	0.0XaaHis: 0.0 ± 0.0	0.0XaaIle: 0.0 ± 0.0	0.0XaaLys: 0.0 ± 0.0	0.0XaaLeu: 0.0 ± 0.0	0.0XaaMet: 0.0 ± 0.0	0.0XaaAsn: 0.0 ± 0.0	0.0XaaPro: 0.0 ± 0.0	0.0XaaGln: 0.0 ± 0.0	0.0XaaArg: 0.0 ± 0.0	0.0XaaSer: 0.0 ± 0.0	0.0XaaThr: 0.0 ± 0.0	0.0XaaVal: 0.0 ± 0.0	0.0XaaTrp: 0.0 ± 0.0	0.0XaaTyr: 0.0 ± 0.0	0.0XaaXaa: 0.0 ± 0.0

Statistics based on 3183 proteins (933435 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944

Contact: Lukasz P. Kozlowski

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