Thalassotalea sp. ND16A
Average proteome isoelectric point is 6.25
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 3946 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A099KG70|A0A099KG70_9GAMM HTH lysR-type domain-containing protein OS=Thalassotalea sp. ND16A OX=1535422 GN=ND16A_2254 PE=3 SV=1
MM1 pKa = 7.05 KK2 pKa = 10.49 QEE4 pKa = 4.01 DD5 pKa = 4.58 QINKK9 pKa = 8.89 DD10 pKa = 2.63 TDD12 pKa = 4.21 KK13 pKa = 10.81 IVKK16 pKa = 5.8 PTRR19 pKa = 11.84 RR20 pKa = 11.84 KK21 pKa = 9.76 FLTRR25 pKa = 11.84 TAAGIVVASIPAKK38 pKa = 10.46 SVFANNVVNSIIASGHH54 pKa = 5.83 ASDD57 pKa = 5.43 FAGGDD62 pKa = 3.94 PIEE65 pKa = 4.63 LLGPCEE71 pKa = 3.99 WLEE74 pKa = 3.94 QDD76 pKa = 5.13 SYY78 pKa = 10.7 WADD81 pKa = 3.08 ISEE84 pKa = 4.37 MPFNSIFGEE93 pKa = 4.69 GPGTSLISLLQCFCCDD109 pKa = 3.91 DD110 pKa = 4.27 PLNFGNDD117 pKa = 3.8 DD118 pKa = 5.7 DD119 pKa = 6.5 DD120 pKa = 6.09 DD121 pKa = 5.51 HH122 pKa = 9.2 DD123 pKa = 4.31 NDD125 pKa = 5.05 NDD127 pKa = 4.19 DD128 pKa = 3.55 GHH130 pKa = 8.14 DD131 pKa = 4.74 NEE133 pKa = 6.5 DD134 pKa = 3.86 EE135 pKa = 4.43 DD136 pKa = 6.26 NEE138 pKa = 5.68 DD139 pKa = 4.91 GDD141 pKa = 6.01 DD142 pKa = 5.6 DD143 pKa = 6.46 GNDD146 pKa = 3.79 DD147 pKa = 5.63 DD148 pKa = 6.71 DD149 pKa = 7.33 DD150 pKa = 4.0 ITATCADD157 pKa = 3.9 DD158 pKa = 4.29 NDD160 pKa = 4.31 YY161 pKa = 11.51 LFAPQMIAMYY171 pKa = 10.48 LNAIVHH177 pKa = 6.12 YY178 pKa = 7.97 NTNGNGPGGLHH189 pKa = 6.16 YY190 pKa = 10.19 PILQNPEE197 pKa = 4.14 DD198 pKa = 4.13 LNGYY202 pKa = 7.93 IQSLIEE208 pKa = 3.93 NRR210 pKa = 11.84 FNLAAIIEE218 pKa = 4.38 EE219 pKa = 4.35 HH220 pKa = 6.69 GGNADD225 pKa = 3.39 PSSFCNVSGNVFDD238 pKa = 6.71 DD239 pKa = 4.52 DD240 pKa = 4.71 DD241 pKa = 3.8
Molecular weight: 26.32 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.694
IPC2_protein 3.579
IPC_protein 3.63
Toseland 3.389
ProMoST 3.795
Dawson 3.643
Bjellqvist 3.821
Wikipedia 3.605
Rodwell 3.452
Grimsley 3.3
Solomon 3.63
Lehninger 3.592
Nozaki 3.757
DTASelect 4.05
Thurlkill 3.452
EMBOSS 3.617
Sillero 3.757
Patrickios 1.202
IPC_peptide 3.63
IPC2_peptide 3.732
IPC2.peptide.svr19 3.718
Protein with the highest isoelectric point:
>tr|A0A099L9R2|A0A099L9R2_9GAMM Uncharacterized protein OS=Thalassotalea sp. ND16A OX=1535422 GN=ND16A_3934 PE=4 SV=1
MM1 pKa = 7.45 KK2 pKa = 9.51 RR3 pKa = 11.84 TFQPSVLKK11 pKa = 10.46 RR12 pKa = 11.84 KK13 pKa = 9.37 RR14 pKa = 11.84 NHH16 pKa = 5.37 GFRR19 pKa = 11.84 ARR21 pKa = 11.84 MATKK25 pKa = 10.33 NGRR28 pKa = 11.84 AVIARR33 pKa = 11.84 RR34 pKa = 11.84 RR35 pKa = 11.84 AKK37 pKa = 10.46 GRR39 pKa = 11.84 AKK41 pKa = 10.69 LSAA44 pKa = 3.92
Molecular weight: 5.08 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.517
IPC2_protein 11.213
IPC_protein 12.793
Toseland 12.969
ProMoST 13.451
Dawson 12.969
Bjellqvist 12.954
Wikipedia 13.437
Rodwell 12.676
Grimsley 12.998
Solomon 13.451
Lehninger 13.364
Nozaki 12.969
DTASelect 12.954
Thurlkill 12.969
EMBOSS 13.466
Sillero 12.969
Patrickios 12.398
IPC_peptide 13.466
IPC2_peptide 12.442
IPC2.peptide.svr19 9.148
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
3946
0
3946
1311202
30
6331
332.3
36.9
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
8.622 ± 0.046
1.022 ± 0.017
5.694 ± 0.052
5.791 ± 0.033
4.344 ± 0.029
6.593 ± 0.042
2.191 ± 0.02
6.849 ± 0.034
5.655 ± 0.045
10.099 ± 0.05
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.427 ± 0.02
5.013 ± 0.032
3.665 ± 0.023
4.785 ± 0.037
3.924 ± 0.03
6.788 ± 0.036
5.49 ± 0.042
6.707 ± 0.035
1.188 ± 0.014
3.155 ± 0.022
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here