Lonestar tick chuvirus 1

Taxonomy: Viruses; Riboviria; Orthornavirae; Negarnaviricota; Haploviricotina; Monjiviricetes; Jingchuvirales; Chuviridae; Mivirus; Mivirus amblyommae

Average proteome isoelectric point is 5.94

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 4 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A172MHP4|A0A172MHP4_9VIRU G OS=Lonestar tick chuvirus 1 OX=1844927 PE=4 SV=1
MM1 pKa = 7.1YY2 pKa = 9.45PSDD5 pKa = 3.53AANVVTAMQTVGTDD19 pKa = 2.56KK20 pKa = 11.32YY21 pKa = 11.06YY22 pKa = 11.14GFKK25 pKa = 10.13RR26 pKa = 11.84DD27 pKa = 3.56LGVVRR32 pKa = 11.84STQYY36 pKa = 11.17KK37 pKa = 9.94SFAFVAKK44 pKa = 9.84MLLQKK49 pKa = 10.87YY50 pKa = 9.87GGAAYY55 pKa = 10.82SNIGQYY61 pKa = 10.13EE62 pKa = 4.58GWPKK66 pKa = 10.8DD67 pKa = 3.0IAGRR71 pKa = 11.84STIEE75 pKa = 3.48EE76 pKa = 4.85AINEE80 pKa = 4.11FNPLSTEE87 pKa = 3.94ADD89 pKa = 3.54DD90 pKa = 4.99AAVTRR95 pKa = 11.84VNILRR100 pKa = 11.84GVAQAATGLDD110 pKa = 3.69SS111 pKa = 4.01

Molecular weight: 12.02 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A172MHQ4|A0A172MHQ4_9VIRU ORF3 OS=Lonestar tick chuvirus 1 OX=1844927 PE=4 SV=1
MM1 pKa = 7.97AEE3 pKa = 4.0QNQPAVQNPPAVDD16 pKa = 3.3AVQAAVAGVHH26 pKa = 5.32ITPPLTDD33 pKa = 3.48AEE35 pKa = 4.32RR36 pKa = 11.84QRR38 pKa = 11.84LAPLVNHH45 pKa = 6.08VNMSRR50 pKa = 11.84AQLVRR55 pKa = 11.84AYY57 pKa = 10.27GGLNIIGNLWGTNTDD72 pKa = 4.63LLPAAATRR80 pKa = 11.84AGAEE84 pKa = 3.94SLMPYY89 pKa = 10.49IMILSKK95 pKa = 11.43GEE97 pKa = 3.99AYY99 pKa = 10.81LLQVDD104 pKa = 4.39AGRR107 pKa = 11.84MRR109 pKa = 11.84VHH111 pKa = 6.39TRR113 pKa = 11.84CIAVANSMVKK123 pKa = 10.24SVGDD127 pKa = 3.1QMPRR131 pKa = 11.84VEE133 pKa = 4.06ATNRR137 pKa = 11.84GVQQVVAFLKK147 pKa = 10.63NDD149 pKa = 3.15IARR152 pKa = 11.84GVEE155 pKa = 3.97DD156 pKa = 4.09MVSISPEE163 pKa = 3.65VLQADD168 pKa = 4.41FEE170 pKa = 4.64GRR172 pKa = 11.84PEE174 pKa = 3.91WLPRR178 pKa = 11.84TRR180 pKa = 11.84DD181 pKa = 3.28EE182 pKa = 4.23LAALAVTEE190 pKa = 4.2AMTVDD195 pKa = 3.37SVVRR199 pKa = 11.84VMNWLYY205 pKa = 11.57DD206 pKa = 4.0HH207 pKa = 7.4IKK209 pKa = 10.44PSAKK213 pKa = 9.96SSGAIIYY220 pKa = 7.96TLGFLALAKK229 pKa = 10.27RR230 pKa = 11.84GTISDD235 pKa = 3.87RR236 pKa = 11.84KK237 pKa = 9.94LSTVMSQMTEE247 pKa = 3.37QGLRR251 pKa = 11.84VVMDD255 pKa = 3.73ISAEE259 pKa = 4.07EE260 pKa = 3.97VKK262 pKa = 9.8MCYY265 pKa = 7.56TQVMSKK271 pKa = 10.21VPHH274 pKa = 5.45EE275 pKa = 4.06HH276 pKa = 4.91VAYY279 pKa = 10.91VFDD282 pKa = 3.47HH283 pKa = 6.61WLTLLGDD290 pKa = 3.5NCLRR294 pKa = 11.84MQLILSQAKK303 pKa = 10.2NSGITILACIKK314 pKa = 9.63QALMMFPTFPLGQNLCDD331 pKa = 3.61VPKK334 pKa = 10.91

Molecular weight: 36.59 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

4

0

4

3299

111

2188

824.8

93.32

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.184 ± 1.468

2.364 ± 0.46

4.82 ± 0.386

6.123 ± 0.552

4.062 ± 0.537

5.092 ± 0.844

2.849 ± 0.526

5.153 ± 0.226

4.941 ± 0.596

9.821 ± 0.654

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.94 ± 0.514

3.062 ± 0.493

4.88 ± 0.323

4.062 ± 0.516

5.547 ± 0.278

7.063 ± 0.772

6.184 ± 0.261

8.154 ± 0.504

1.364 ± 0.091

4.335 ± 0.46
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski