Arcticibacter tournemirensis
Average proteome isoelectric point is 6.85
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 4170 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A4Q0ME70|A0A4Q0ME70_9SPHI Threonine/serine exporter OS=Arcticibacter tournemirensis OX=699437 GN=EKH83_03270 PE=4 SV=1
MM1 pKa = 7.72 NDD3 pKa = 2.89 NKK5 pKa = 10.28 FNYY8 pKa = 9.02 KK9 pKa = 10.47 DD10 pKa = 3.31 QVLNIACMLSTVYY23 pKa = 10.48 RR24 pKa = 11.84 EE25 pKa = 3.83 LFIYY29 pKa = 9.93 GLNAALHH36 pKa = 6.29 NEE38 pKa = 4.1 LKK40 pKa = 10.43 DD41 pKa = 3.29 IKK43 pKa = 10.92 DD44 pKa = 3.13 IFEE47 pKa = 5.26 DD48 pKa = 4.24 GEE50 pKa = 4.42 EE51 pKa = 4.36 YY52 pKa = 10.44 PGDD55 pKa = 3.84 VALLEE60 pKa = 4.52 ALDD63 pKa = 4.69 DD64 pKa = 4.26 EE65 pKa = 4.88 NIKK68 pKa = 10.57 IILSAMYY75 pKa = 10.13 DD76 pKa = 3.11 IEE78 pKa = 4.68 EE79 pKa = 4.58 YY80 pKa = 11.07 GDD82 pKa = 3.68 SLLNINNISDD92 pKa = 3.65 KK93 pKa = 10.62 EE94 pKa = 4.13 LNEE97 pKa = 3.93 GLEE100 pKa = 4.19 NGLGSEE106 pKa = 4.3 YY107 pKa = 11.05 APDD110 pKa = 3.37 YY111 pKa = 11.49 GEE113 pKa = 5.19 AVEE116 pKa = 3.94 NDD118 pKa = 2.95 YY119 pKa = 11.25 RR120 pKa = 11.84 FWLNEE125 pKa = 3.58 VMGYY129 pKa = 6.94 THH131 pKa = 7.4 LSVFNLLTLCYY142 pKa = 10.13 SLSNGVNEE150 pKa = 4.23 VPEE153 pKa = 4.09 EE154 pKa = 4.26 HH155 pKa = 7.05 VSSLYY160 pKa = 10.77 FPTDD164 pKa = 2.88 EE165 pKa = 5.01 SLALLDD171 pKa = 5.4 IGDD174 pKa = 4.09 QNVKK178 pKa = 10.51 LLTEE182 pKa = 4.34 LAFKK186 pKa = 11.0 LSDD189 pKa = 3.96 CANDD193 pKa = 3.58 LCEE196 pKa = 4.56 KK197 pKa = 10.68 LL198 pKa = 3.88
Molecular weight: 22.44 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.799
IPC2_protein 3.91
IPC_protein 3.872
Toseland 3.681
ProMoST 4.012
Dawson 3.846
Bjellqvist 3.999
Wikipedia 3.745
Rodwell 3.706
Grimsley 3.592
Solomon 3.834
Lehninger 3.795
Nozaki 3.961
DTASelect 4.139
Thurlkill 3.719
EMBOSS 3.757
Sillero 3.986
Patrickios 0.579
IPC_peptide 3.846
IPC2_peptide 3.973
IPC2.peptide.svr19 3.888
Protein with the highest isoelectric point:
>tr|A0A4Q0M5L6|A0A4Q0M5L6_9SPHI Rod shape-determining protein RodA OS=Arcticibacter tournemirensis OX=699437 GN=rodA PE=4 SV=1
MM1 pKa = 8.15 RR2 pKa = 11.84 YY3 pKa = 8.82 FLCIILPPVAVLTTGRR19 pKa = 11.84 IGALILNIVLCLFFWIPGVIHH40 pKa = 7.41 AILVTNDD47 pKa = 2.94 YY48 pKa = 11.01 YY49 pKa = 11.5 ADD51 pKa = 3.36 KK52 pKa = 10.76 RR53 pKa = 11.84 NRR55 pKa = 11.84 KK56 pKa = 9.32 LIRR59 pKa = 11.84 AVKK62 pKa = 10.19 NSRR65 pKa = 3.18
Molecular weight: 7.46 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.332
IPC2_protein 9.794
IPC_protein 10.482
Toseland 10.365
ProMoST 10.452
Dawson 10.555
Bjellqvist 10.292
Wikipedia 10.774
Rodwell 10.789
Grimsley 10.628
Solomon 10.628
Lehninger 10.584
Nozaki 10.394
DTASelect 10.277
Thurlkill 10.409
EMBOSS 10.76
Sillero 10.482
Patrickios 10.599
IPC_peptide 10.613
IPC2_peptide 9.487
IPC2.peptide.svr19 8.467
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
4170
0
4170
1573326
25
4255
377.3
42.35
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
7.185 ± 0.033
0.79 ± 0.013
5.328 ± 0.025
5.926 ± 0.037
4.909 ± 0.023
7.063 ± 0.034
1.728 ± 0.017
7.015 ± 0.033
6.694 ± 0.035
9.358 ± 0.038
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.188 ± 0.015
5.472 ± 0.034
3.877 ± 0.021
3.424 ± 0.017
4.575 ± 0.026
6.969 ± 0.035
5.54 ± 0.034
6.435 ± 0.022
1.283 ± 0.014
4.239 ± 0.027
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here