Ilyomonas limi

Taxonomy: cellular organisms; Bacteria; FCB group; Bacteroidetes/Chlorobi group; Bacteroidetes; Chitinophagia; Chitinophagales; Chitinophagaceae; Ilyomonas

Average proteome isoelectric point is 6.78

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 4606 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A4U3L2S8|A0A4U3L2S8_9BACT ATP-dependent DNA helicase UvrD2 OS=Ilyomonas limi OX=2575867 GN=FC093_10505 PE=4 SV=1
MM1 pKa = 7.23QPDD4 pKa = 4.02YY5 pKa = 11.2NQLWRR10 pKa = 11.84NMNNGQTPDD19 pKa = 3.24AQFYY23 pKa = 11.38ALFEE27 pKa = 5.06DD28 pKa = 4.64SLNQFISTNSNVAQAANNAVEE49 pKa = 4.53QNNSDD54 pKa = 3.84HH55 pKa = 6.52NVVNAEE61 pKa = 3.87

Molecular weight: 6.9 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A4U3KTI3|A0A4U3KTI3_9BACT Cytokinin riboside 5'-monophosphate phosphoribohydrolase OS=Ilyomonas limi OX=2575867 GN=FC093_19135 PE=3 SV=1
MM1 pKa = 7.48LSLWFISANHH11 pKa = 6.18PKK13 pKa = 10.05IVGNGEE19 pKa = 3.96FPSLQTCRR27 pKa = 11.84RR28 pKa = 11.84YY29 pKa = 9.02FQQWTKK35 pKa = 10.6EE36 pKa = 4.05GVIKK40 pKa = 10.8RR41 pKa = 11.84MLRR44 pKa = 11.84TLGCFHH50 pKa = 7.22ARR52 pKa = 3.47

Molecular weight: 6.15 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

4606

0

4606

1624473

28

2416

352.7

39.57

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.904 ± 0.035

0.931 ± 0.011

5.243 ± 0.029

5.316 ± 0.035

4.779 ± 0.028

6.649 ± 0.036

2.052 ± 0.016

7.167 ± 0.031

6.628 ± 0.032

9.176 ± 0.039

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.363 ± 0.019

5.822 ± 0.035

3.93 ± 0.018

4.155 ± 0.024

3.768 ± 0.023

6.02 ± 0.027

6.023 ± 0.029

6.366 ± 0.03

1.338 ± 0.015

4.37 ± 0.028
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski