Tortoise microvirus 39
Average proteome isoelectric point is 7.66
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 7 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A4P8W6F9|A0A4P8W6F9_9VIRU Uncharacterized protein OS=Tortoise microvirus 39 OX=2583142 PE=4 SV=1
MM1 pKa = 7.21 RR2 pKa = 11.84 TSPVPVQRR10 pKa = 11.84 TMRR13 pKa = 11.84 RR14 pKa = 11.84 HH15 pKa = 5.58 DD16 pKa = 4.11 LKK18 pKa = 11.47 VLTSLPAGKK27 pKa = 9.52 FVPLAAVPLLRR38 pKa = 11.84 EE39 pKa = 4.22 DD40 pKa = 3.63 AVTRR44 pKa = 11.84 LNGTISFEE52 pKa = 3.85 MSEE55 pKa = 4.26 TVEE58 pKa = 3.79 ILMNAVNVRR67 pKa = 11.84 VMAYY71 pKa = 9.58 LVPFLAFEE79 pKa = 4.64 RR80 pKa = 11.84 FAGMDD85 pKa = 3.76 DD86 pKa = 4.68 LNRR89 pKa = 11.84 SYY91 pKa = 11.4 QKK93 pKa = 11.03 VPMKK97 pKa = 10.79 DD98 pKa = 3.39 GDD100 pKa = 3.82 PVIPFFEE107 pKa = 4.78 SMVAPAHH114 pKa = 5.66 GANPILTYY122 pKa = 9.63 MGKK125 pKa = 8.94 HH126 pKa = 5.81 CRR128 pKa = 11.84 PGTNINSAYY137 pKa = 9.63 IEE139 pKa = 4.61 AYY141 pKa = 8.58 NAVWNFRR148 pKa = 11.84 AKK150 pKa = 10.38 NRR152 pKa = 11.84 SPNITQRR159 pKa = 11.84 TRR161 pKa = 11.84 LEE163 pKa = 3.92 ATLAPAFWLHH173 pKa = 6.62 SNFNHH178 pKa = 5.72 VVPDD182 pKa = 3.91 FDD184 pKa = 3.59 QAVIDD189 pKa = 4.87 GEE191 pKa = 4.48 VALNSAQVNLALKK204 pKa = 10.19 NADD207 pKa = 3.86 GTPGSAPVSVTGASLTVNSATSSTRR232 pKa = 11.84 AVQMNNTGQLVYY244 pKa = 10.72 AGSPLAAAAGVTGLGGLTADD264 pKa = 4.85 LAAITAEE271 pKa = 4.07 LQQDD275 pKa = 4.91 GITISLSNIEE285 pKa = 4.05 LARR288 pKa = 11.84 RR289 pKa = 11.84 TQAFAKK295 pKa = 10.31 LRR297 pKa = 11.84 AQYY300 pKa = 9.85 HH301 pKa = 4.72 GHH303 pKa = 5.68 SDD305 pKa = 3.46 EE306 pKa = 4.76 YY307 pKa = 10.8 IIDD310 pKa = 4.05 LLMSGISVPEE320 pKa = 4.03 QAWKK324 pKa = 10.57 QPILLADD331 pKa = 3.37 EE332 pKa = 4.24 HH333 pKa = 6.95 TIFGMSKK340 pKa = 10.01 RR341 pKa = 11.84 YY342 pKa = 10.09 ASDD345 pKa = 3.21 AANLTEE351 pKa = 4.22 SVVNGGTVIDD361 pKa = 4.8 LSVSVPRR368 pKa = 11.84 VPTGGVVMLFAEE380 pKa = 4.62 ITPEE384 pKa = 3.85 QLFEE388 pKa = 4.21 RR389 pKa = 11.84 MKK391 pKa = 11.25 DD392 pKa = 3.66 PFLHH396 pKa = 7.02 ALDD399 pKa = 4.83 PQDD402 pKa = 4.94 DD403 pKa = 4.06 LPEE406 pKa = 4.27 FVRR409 pKa = 11.84 DD410 pKa = 3.48 TLDD413 pKa = 3.51 PEE415 pKa = 4.37 KK416 pKa = 11.04 VSVVPCEE423 pKa = 4.15 YY424 pKa = 11.11 VDD426 pKa = 4.11 MDD428 pKa = 4.69 HH429 pKa = 7.26 DD430 pKa = 4.49 TPDD433 pKa = 3.27 STFGYY438 pKa = 10.89 APLNYY443 pKa = 9.41 EE444 pKa = 3.95 WAQNSPQIGGRR455 pKa = 11.84 FYY457 pKa = 11.2 RR458 pKa = 11.84 PAVDD462 pKa = 3.43 AAFDD466 pKa = 3.88 EE467 pKa = 4.45 DD468 pKa = 3.97 RR469 pKa = 11.84 QRR471 pKa = 11.84 IWAVEE476 pKa = 4.16 TQNPTLSEE484 pKa = 3.99 DD485 pKa = 4.19 FYY487 pKa = 11.6 LCTTMHH493 pKa = 6.09 QKK495 pKa = 10.5 PFVVTNVDD503 pKa = 3.2 PFEE506 pKa = 5.02 ALLRR510 pKa = 11.84 GEE512 pKa = 5.11 AIISGNTVFGGLLVEE527 pKa = 5.22 ANDD530 pKa = 4.76 DD531 pKa = 3.98 YY532 pKa = 12.24 AEE534 pKa = 4.31 VAAKK538 pKa = 10.64 APVDD542 pKa = 4.49 RR543 pKa = 11.84 IVKK546 pKa = 9.08 PAA548 pKa = 3.47
Molecular weight: 59.84 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 4.869
IPC2_protein 4.863
IPC_protein 4.825
Toseland 4.724
ProMoST 4.965
Dawson 4.813
Bjellqvist 4.952
Wikipedia 4.698
Rodwell 4.711
Grimsley 4.635
Solomon 4.813
Lehninger 4.762
Nozaki 4.927
DTASelect 5.105
Thurlkill 4.724
EMBOSS 4.724
Sillero 4.991
Patrickios 4.024
IPC_peptide 4.813
IPC2_peptide 4.978
IPC2.peptide.svr19 4.938
Protein with the highest isoelectric point:
>tr|A0A4P8W9P3|A0A4P8W9P3_9VIRU Uncharacterized protein OS=Tortoise microvirus 39 OX=2583142 PE=4 SV=1
MM1 pKa = 7.58 EE2 pKa = 5.55 GNMSKK7 pKa = 10.72 SNKK10 pKa = 8.22 EE11 pKa = 3.32 RR12 pKa = 11.84 QAQYY16 pKa = 10.27 RR17 pKa = 11.84 AKK19 pKa = 10.56 LEE21 pKa = 4.27 SLGQTNVTGIVNYY34 pKa = 8.01 TQVGDD39 pKa = 3.7 VLILLRR45 pKa = 11.84 RR46 pKa = 11.84 LRR48 pKa = 11.84 DD49 pKa = 3.7 NPDD52 pKa = 3.39 LEE54 pKa = 4.51 VGLIRR59 pKa = 11.84 NTRR62 pKa = 11.84 TGRR65 pKa = 11.84 YY66 pKa = 7.34 EE67 pKa = 3.79 KK68 pKa = 10.96 LL69 pKa = 3.29
Molecular weight: 7.92 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.147
IPC2_protein 9.297
IPC_protein 9.56
Toseland 9.94
ProMoST 9.692
Dawson 10.175
Bjellqvist 9.867
Wikipedia 10.365
Rodwell 10.423
Grimsley 10.262
Solomon 10.233
Lehninger 10.204
Nozaki 9.897
DTASelect 9.867
Thurlkill 9.999
EMBOSS 10.35
Sillero 10.072
Patrickios 10.116
IPC_peptide 10.233
IPC2_peptide 8.478
IPC2.peptide.svr19 8.364
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
7
0
7
1717
67
548
245.3
27.37
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
10.25 ± 0.836
0.815 ± 0.43
5.649 ± 0.364
5.591 ± 0.684
3.844 ± 0.372
7.804 ± 0.775
2.854 ± 0.616
3.378 ± 0.328
3.902 ± 0.365
7.921 ± 0.476
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.97 ± 0.289
4.601 ± 0.497
5.591 ± 0.592
4.252 ± 0.349
7.338 ± 0.77
4.543 ± 0.535
6.057 ± 0.5
7.455 ± 0.735
1.864 ± 0.381
3.32 ± 0.792
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here