Aquamicrobium defluvii

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Alphaproteobacteria; Hyphomicrobiales; Phyllobacteriaceae; Aquamicrobium

Average proteome isoelectric point is 6.55

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 4454 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A011U0V1|A0A011U0V1_9RHIZ Uncharacterized protein OS=Aquamicrobium defluvii OX=69279 GN=BG36_14410 PE=4 SV=1
MM1 pKa = 7.83HH2 pKa = 7.4MDD4 pKa = 3.07STTEE8 pKa = 4.02IIAHH12 pKa = 6.5FIGVFSQTVEE22 pKa = 4.08DD23 pKa = 3.55VRR25 pKa = 11.84LRR27 pKa = 11.84LDD29 pKa = 3.42YY30 pKa = 11.42AKK32 pKa = 10.49FAAEE36 pKa = 3.95RR37 pKa = 11.84EE38 pKa = 4.47APPEE42 pKa = 4.15TGDD45 pKa = 4.49LLDD48 pKa = 4.59IKK50 pKa = 10.66LTMTSPYY57 pKa = 10.11TLGQFAPKK65 pKa = 8.6VTYY68 pKa = 10.27APPSFDD74 pKa = 2.88IVPTVAISFLSHH86 pKa = 6.0QPVPLHH92 pKa = 5.83MPPGNMTKK100 pKa = 10.12VFAAPHH106 pKa = 4.5QHH108 pKa = 6.09HH109 pKa = 6.52MPGMSEE115 pKa = 4.13RR116 pKa = 11.84MIPEE120 pKa = 3.93YY121 pKa = 10.88EE122 pKa = 4.66PIGSLALYY130 pKa = 9.84LRR132 pKa = 11.84QEE134 pKa = 3.88AHH136 pKa = 6.08LTDD139 pKa = 4.07NDD141 pKa = 3.73MLSIGDD147 pKa = 4.28HH148 pKa = 5.76GLAMKK153 pKa = 10.65AVGDD157 pKa = 4.05PVAALAKK164 pKa = 10.68LMGAASQLQPLADD177 pKa = 3.66MDD179 pKa = 4.34GLHH182 pKa = 6.29TEE184 pKa = 4.04ADD186 pKa = 3.06AGGFIQHH193 pKa = 5.47VTQAVDD199 pKa = 3.7TLPDD203 pKa = 3.7AAPAGGTLFVAQGAAAAGIHH223 pKa = 5.59VNGEE227 pKa = 4.38SVEE230 pKa = 4.1EE231 pKa = 4.13APVLEE236 pKa = 5.03DD237 pKa = 3.82LLPAKK242 pKa = 8.6FQPPAEE248 pKa = 4.28EE249 pKa = 3.96TSEE252 pKa = 5.48DD253 pKa = 3.83GDD255 pKa = 3.61GDD257 pKa = 4.02APVPTGVDD265 pKa = 3.45YY266 pKa = 11.32SLSSMIEE273 pKa = 3.86LEE275 pKa = 4.1AGGNLLVNEE284 pKa = 4.46AVLANNWYY292 pKa = 9.51GVPVVAAAGDD302 pKa = 4.07YY303 pKa = 9.79VHH305 pKa = 6.8VNAISQTNVWSDD317 pKa = 3.45CDD319 pKa = 3.7AVSDD323 pKa = 5.5ALGGWQRR330 pKa = 11.84LTDD333 pKa = 3.7TPTQAFNVATIEE345 pKa = 4.37TIANPAYY352 pKa = 10.39GEE354 pKa = 4.15AAGGAGFPSYY364 pKa = 9.67WQVSRR369 pKa = 11.84IDD371 pKa = 3.66GDD373 pKa = 4.22LLIANWIKK381 pKa = 10.4QVSFVQDD388 pKa = 2.82NDD390 pKa = 3.65VTVMSACGGGTTITLGDD407 pKa = 3.62NTVVNIASLAEE418 pKa = 3.96LGVYY422 pKa = 10.1YY423 pKa = 10.57DD424 pKa = 4.82LIIVGGSIYY433 pKa = 10.19HH434 pKa = 6.31GNIISQTNILLDD446 pKa = 4.59DD447 pKa = 4.85DD448 pKa = 5.21LVGTVGDD455 pKa = 4.05FQTSGEE461 pKa = 4.18ASLSTGGNLLWNQASITQYY480 pKa = 9.68GTMSFEE486 pKa = 4.15GLPPSFQAAADD497 pKa = 3.94TLASGPADD505 pKa = 3.75APDD508 pKa = 4.41DD509 pKa = 4.26LLHH512 pKa = 6.78HH513 pKa = 6.31EE514 pKa = 4.86AFGGLAGLKK523 pKa = 9.69VLYY526 pKa = 10.28ISGDD530 pKa = 3.85VIDD533 pKa = 5.02LQYY536 pKa = 11.04ISQTNILGDD545 pKa = 3.89ADD547 pKa = 3.98QVALAMDD554 pKa = 3.58QAGAYY559 pKa = 10.0ADD561 pKa = 4.53ADD563 pKa = 3.89WTVSTGTNALVNYY576 pKa = 9.51AHH578 pKa = 6.99IVDD581 pKa = 4.53GGVDD585 pKa = 3.47STVYY589 pKa = 10.78AGGDD593 pKa = 3.61VYY595 pKa = 11.33SDD597 pKa = 3.58EE598 pKa = 5.43LLIQAEE604 pKa = 4.8LISNDD609 pKa = 3.12SGLYY613 pKa = 8.5MQDD616 pKa = 2.11ATALVNEE623 pKa = 4.31AVVFLSDD630 pKa = 5.54DD631 pKa = 3.81LLAPDD636 pKa = 4.82AADD639 pKa = 4.52DD640 pKa = 4.17GAPVHH645 pKa = 7.16DD646 pKa = 4.62GAGPGAGSADD656 pKa = 3.52VMQTMLSS663 pKa = 3.51

Molecular weight: 69.25 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A011UD08|A0A011UD08_9RHIZ Arsenic transporter OS=Aquamicrobium defluvii OX=69279 GN=BG36_11185 PE=3 SV=1
MM1 pKa = 7.35KK2 pKa = 9.43RR3 pKa = 11.84TYY5 pKa = 10.27QPSKK9 pKa = 9.73LVRR12 pKa = 11.84KK13 pKa = 9.15RR14 pKa = 11.84RR15 pKa = 11.84HH16 pKa = 4.42GFRR19 pKa = 11.84ARR21 pKa = 11.84MATNGGRR28 pKa = 11.84AVIAARR34 pKa = 11.84RR35 pKa = 11.84NRR37 pKa = 11.84GRR39 pKa = 11.84KK40 pKa = 9.03RR41 pKa = 11.84LSAA44 pKa = 4.03

Molecular weight: 5.14 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

4454

0

4454

1348077

30

3895

302.7

32.88

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

12.404 ± 0.051

0.812 ± 0.011

5.676 ± 0.032

5.94 ± 0.038

3.763 ± 0.022

8.731 ± 0.073

2.039 ± 0.017

5.356 ± 0.026

3.223 ± 0.03

9.886 ± 0.042

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.624 ± 0.019

2.593 ± 0.02

5.002 ± 0.031

3.054 ± 0.019

7.397 ± 0.045

5.442 ± 0.028

5.097 ± 0.021

7.453 ± 0.027

1.298 ± 0.012

2.211 ± 0.019
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski