Staphylococcus phage SP276

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; Coventryvirus; Staphylococcus virus SP276

Average proteome isoelectric point is 6.27

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 70 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A494WJD4|A0A494WJD4_9CAUD Phage tail length tape-measure protein OS=Staphylococcus phage SP276 OX=2495555 GN=SP276g_01 PE=4 SV=1
MM1 pKa = 7.65LEE3 pKa = 4.18STPQQALYY11 pKa = 10.6DD12 pKa = 4.09YY13 pKa = 10.95IFTQLLGYY21 pKa = 9.93GIDD24 pKa = 3.63VIDD27 pKa = 4.23FKK29 pKa = 11.33EE30 pKa = 4.36LNSQLTYY37 pKa = 10.72PFFVLRR43 pKa = 11.84DD44 pKa = 3.69VEE46 pKa = 4.52VSKK49 pKa = 11.17SKK51 pKa = 10.02YY52 pKa = 7.58TMEE55 pKa = 4.21NVGGEE60 pKa = 4.13LTVTIDD66 pKa = 3.17LWNYY70 pKa = 10.02ADD72 pKa = 6.27DD73 pKa = 5.07RR74 pKa = 11.84GQHH77 pKa = 6.16DD78 pKa = 4.61SIVYY82 pKa = 10.21AIEE85 pKa = 4.11SMLTVIEE92 pKa = 4.41SVEE95 pKa = 4.33SYY97 pKa = 11.07QLILDD102 pKa = 3.75GMIIKK107 pKa = 8.63TLNDD111 pKa = 3.23IEE113 pKa = 4.59NSDD116 pKa = 3.99RR117 pKa = 11.84QLLHH121 pKa = 5.52TVIIATYY128 pKa = 10.72KK129 pKa = 10.76LFF131 pKa = 4.19

Molecular weight: 15.1 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A494WAJ2|A0A494WAJ2_9CAUD Uncharacterized protein OS=Staphylococcus phage SP276 OX=2495555 GN=SP276g_53 PE=4 SV=1
MM1 pKa = 7.44ILSNSVRR8 pKa = 11.84QRR10 pKa = 11.84YY11 pKa = 7.38RR12 pKa = 11.84TDD14 pKa = 3.24TVGKK18 pKa = 8.39TPTEE22 pKa = 3.9LQKK25 pKa = 10.55EE26 pKa = 4.12LRR28 pKa = 11.84KK29 pKa = 10.13RR30 pKa = 11.84GVRR33 pKa = 11.84GFVVNVSHH41 pKa = 6.88NRR43 pKa = 11.84VTMLVDD49 pKa = 3.23RR50 pKa = 11.84RR51 pKa = 11.84DD52 pKa = 3.25VKK54 pKa = 9.98TNKK57 pKa = 9.82EE58 pKa = 3.97CMRR61 pKa = 11.84CLTSTEE67 pKa = 3.88TRR69 pKa = 11.84KK70 pKa = 10.33

Molecular weight: 8.22 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

70

0

70

12632

47

847

180.5

20.7

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

5.708 ± 0.39

0.451 ± 0.075

6.539 ± 0.332

7.513 ± 0.486

4.314 ± 0.215

4.987 ± 0.307

1.734 ± 0.179

7.576 ± 0.32

9.547 ± 0.403

8.027 ± 0.26

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.383 ± 0.123

6.737 ± 0.251

2.612 ± 0.184

3.927 ± 0.194

3.887 ± 0.249

6.119 ± 0.26

6.127 ± 0.274

6.42 ± 0.257

1.061 ± 0.125

4.33 ± 0.258
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski