Amino acid dipepetide frequency for Staphylococcus phage SP276

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
1.9AlaAla: 1.9 ± 0.383
0.317AlaCys: 0.317 ± 0.141
3.721AlaAsp: 3.721 ± 0.502
4.275AlaGlu: 4.275 ± 0.595
2.375AlaPhe: 2.375 ± 0.52
2.692AlaGly: 2.692 ± 0.454
0.792AlaHis: 0.792 ± 0.263
6.413AlaIle: 6.413 ± 1.037
5.463AlaLys: 5.463 ± 0.771
4.75AlaLeu: 4.75 ± 0.758
1.188AlaMet: 1.188 ± 0.325
4.275AlaAsn: 4.275 ± 0.582
1.188AlaPro: 1.188 ± 0.291
2.533AlaGln: 2.533 ± 0.41
2.138AlaArg: 2.138 ± 0.463
2.692AlaSer: 2.692 ± 0.483
4.513AlaThr: 4.513 ± 0.637
3.325AlaVal: 3.325 ± 0.647
0.633AlaTrp: 0.633 ± 0.224
1.9AlaTyr: 1.9 ± 0.355
0.0AlaXaa: 0.0 ± 0.0
Cys
0.158CysAla: 0.158 ± 0.114
0.0CysCys: 0.0 ± 0.0
0.238CysAsp: 0.238 ± 0.131
0.079CysGlu: 0.079 ± 0.082
0.0CysPhe: 0.0 ± 0.0
0.238CysGly: 0.238 ± 0.136
0.238CysHis: 0.238 ± 0.151
0.317CysIle: 0.317 ± 0.15
0.475CysLys: 0.475 ± 0.162
0.475CysLeu: 0.475 ± 0.211
0.238CysMet: 0.238 ± 0.144
0.633CysAsn: 0.633 ± 0.228
0.079CysPro: 0.079 ± 0.082
0.079CysGln: 0.079 ± 0.082
0.396CysArg: 0.396 ± 0.18
0.317CysSer: 0.317 ± 0.147
0.317CysThr: 0.317 ± 0.148
0.0CysVal: 0.0 ± 0.0
0.079CysTrp: 0.079 ± 0.083
0.158CysTyr: 0.158 ± 0.12
0.0CysXaa: 0.0 ± 0.0
Asp
3.563AspAla: 3.563 ± 0.587
0.079AspCys: 0.079 ± 0.08
5.7AspAsp: 5.7 ± 0.917
5.938AspGlu: 5.938 ± 0.876
3.404AspPhe: 3.404 ± 0.565
4.038AspGly: 4.038 ± 0.583
0.871AspHis: 0.871 ± 0.271
4.196AspIle: 4.196 ± 0.639
6.096AspLys: 6.096 ± 0.792
5.7AspLeu: 5.7 ± 0.809
1.742AspMet: 1.742 ± 0.363
4.038AspAsn: 4.038 ± 0.546
1.108AspPro: 1.108 ± 0.229
0.713AspGln: 0.713 ± 0.229
2.058AspArg: 2.058 ± 0.365
4.592AspSer: 4.592 ± 0.718
2.85AspThr: 2.85 ± 0.407
4.671AspVal: 4.671 ± 0.561
0.475AspTrp: 0.475 ± 0.203
3.563AspTyr: 3.563 ± 0.635
0.0AspXaa: 0.0 ± 0.0
Glu
5.542GluAla: 5.542 ± 0.597
0.633GluCys: 0.633 ± 0.228
3.404GluAsp: 3.404 ± 0.562
7.046GluGlu: 7.046 ± 0.947
4.434GluPhe: 4.434 ± 0.556
2.533GluGly: 2.533 ± 0.396
0.633GluHis: 0.633 ± 0.216
4.988GluIle: 4.988 ± 0.726
5.067GluLys: 5.067 ± 0.65
6.729GluLeu: 6.729 ± 0.787
2.454GluMet: 2.454 ± 0.408
4.354GluAsn: 4.354 ± 0.718
1.663GluPro: 1.663 ± 0.423
3.879GluGln: 3.879 ± 0.535
3.879GluArg: 3.879 ± 0.613
5.146GluSer: 5.146 ± 0.885
3.246GluThr: 3.246 ± 0.621
5.384GluVal: 5.384 ± 0.739
0.633GluTrp: 0.633 ± 0.233
3.483GluTyr: 3.483 ± 0.528
0.0GluXaa: 0.0 ± 0.0
Phe
2.058PheAla: 2.058 ± 0.465
0.158PheCys: 0.158 ± 0.096
2.454PheAsp: 2.454 ± 0.519
4.275PheGlu: 4.275 ± 0.745
1.821PhePhe: 1.821 ± 0.401
3.008PheGly: 3.008 ± 0.777
0.475PheHis: 0.475 ± 0.183
3.167PheIle: 3.167 ± 0.513
4.75PheLys: 4.75 ± 0.614
2.692PheLeu: 2.692 ± 0.498
1.188PheMet: 1.188 ± 0.279
3.325PheAsn: 3.325 ± 0.595
0.95PhePro: 0.95 ± 0.228
1.267PheGln: 1.267 ± 0.315
1.188PheArg: 1.188 ± 0.309
2.058PheSer: 2.058 ± 0.412
2.454PheThr: 2.454 ± 0.367
3.642PheVal: 3.642 ± 0.6
0.317PheTrp: 0.317 ± 0.153
1.9PheTyr: 1.9 ± 0.416
0.0PheXaa: 0.0 ± 0.0
Gly
2.692GlyAla: 2.692 ± 0.966
0.396GlyCys: 0.396 ± 0.171
2.692GlyAsp: 2.692 ± 0.415
3.642GlyGlu: 3.642 ± 0.612
2.454GlyPhe: 2.454 ± 0.446
2.138GlyGly: 2.138 ± 0.391
1.029GlyHis: 1.029 ± 0.273
3.959GlyIle: 3.959 ± 0.604
4.434GlyLys: 4.434 ± 0.791
4.196GlyLeu: 4.196 ± 0.794
1.267GlyMet: 1.267 ± 0.299
2.375GlyAsn: 2.375 ± 0.367
0.238GlyPro: 0.238 ± 0.166
1.9GlyGln: 1.9 ± 0.377
2.375GlyArg: 2.375 ± 0.427
2.613GlySer: 2.613 ± 0.338
3.642GlyThr: 3.642 ± 0.473
4.592GlyVal: 4.592 ± 0.751
0.396GlyTrp: 0.396 ± 0.193
2.85GlyTyr: 2.85 ± 0.505
0.0GlyXaa: 0.0 ± 0.0
His
0.396HisAla: 0.396 ± 0.174
0.317HisCys: 0.317 ± 0.133
1.108HisAsp: 1.108 ± 0.325
0.713HisGlu: 0.713 ± 0.217
1.188HisPhe: 1.188 ± 0.409
1.267HisGly: 1.267 ± 0.345
0.317HisHis: 0.317 ± 0.166
1.583HisIle: 1.583 ± 0.397
1.425HisLys: 1.425 ± 0.344
1.663HisLeu: 1.663 ± 0.381
0.317HisMet: 0.317 ± 0.154
1.029HisAsn: 1.029 ± 0.319
0.396HisPro: 0.396 ± 0.185
1.267HisGln: 1.267 ± 0.308
0.396HisArg: 0.396 ± 0.18
0.871HisSer: 0.871 ± 0.289
0.95HisThr: 0.95 ± 0.328
1.108HisVal: 1.108 ± 0.315
0.079HisTrp: 0.079 ± 0.077
0.95HisTyr: 0.95 ± 0.291
0.0HisXaa: 0.0 ± 0.0
Ile
4.513IleAla: 4.513 ± 0.741
0.396IleCys: 0.396 ± 0.172
6.492IleAsp: 6.492 ± 0.617
5.384IleGlu: 5.384 ± 0.803
2.692IlePhe: 2.692 ± 0.533
3.483IleGly: 3.483 ± 0.8
1.108IleHis: 1.108 ± 0.312
4.513IleIle: 4.513 ± 0.762
8.471IleLys: 8.471 ± 0.836
4.75IleLeu: 4.75 ± 0.686
1.029IleMet: 1.029 ± 0.343
6.096IleAsn: 6.096 ± 0.706
3.325IlePro: 3.325 ± 0.453
2.85IleGln: 2.85 ± 0.561
2.771IleArg: 2.771 ± 0.485
4.434IleSer: 4.434 ± 0.563
4.513IleThr: 4.513 ± 0.598
4.592IleVal: 4.592 ± 0.592
0.871IleTrp: 0.871 ± 0.233
3.483IleTyr: 3.483 ± 0.474
0.0IleXaa: 0.0 ± 0.0
Lys
5.304LysAla: 5.304 ± 0.598
0.238LysCys: 0.238 ± 0.135
6.175LysAsp: 6.175 ± 0.915
7.521LysGlu: 7.521 ± 1.145
3.8LysPhe: 3.8 ± 0.588
5.067LysGly: 5.067 ± 0.693
1.346LysHis: 1.346 ± 0.313
8.313LysIle: 8.313 ± 0.705
7.917LysLys: 7.917 ± 1.023
7.68LysLeu: 7.68 ± 0.773
2.613LysMet: 2.613 ± 0.532
4.75LysAsn: 4.75 ± 0.56
2.692LysPro: 2.692 ± 0.886
5.938LysGln: 5.938 ± 0.734
4.434LysArg: 4.434 ± 0.66
5.225LysSer: 5.225 ± 0.728
5.859LysThr: 5.859 ± 0.734
5.859LysVal: 5.859 ± 0.67
0.95LysTrp: 0.95 ± 0.227
3.8LysTyr: 3.8 ± 0.575
0.0LysXaa: 0.0 ± 0.0
Leu
4.671LeuAla: 4.671 ± 0.614
0.158LeuCys: 0.158 ± 0.185
5.542LeuAsp: 5.542 ± 0.717
5.7LeuGlu: 5.7 ± 0.745
3.008LeuPhe: 3.008 ± 0.463
3.563LeuGly: 3.563 ± 0.736
1.583LeuHis: 1.583 ± 0.306
6.65LeuIle: 6.65 ± 0.813
8.471LeuLys: 8.471 ± 0.782
6.175LeuLeu: 6.175 ± 0.763
1.9LeuMet: 1.9 ± 0.384
6.096LeuAsn: 6.096 ± 0.79
2.217LeuPro: 2.217 ± 0.419
2.692LeuGln: 2.692 ± 0.383
3.404LeuArg: 3.404 ± 0.73
5.146LeuSer: 5.146 ± 0.918
6.175LeuThr: 6.175 ± 0.845
3.721LeuVal: 3.721 ± 0.678
1.267LeuTrp: 1.267 ± 0.443
2.138LeuTyr: 2.138 ± 0.355
0.0LeuXaa: 0.0 ± 0.0
Met
1.663MetAla: 1.663 ± 0.408
0.079MetCys: 0.079 ± 0.083
1.346MetAsp: 1.346 ± 0.315
2.454MetGlu: 2.454 ± 0.515
1.029MetPhe: 1.029 ± 0.305
0.792MetGly: 0.792 ± 0.245
0.554MetHis: 0.554 ± 0.191
2.138MetIle: 2.138 ± 0.477
2.138MetLys: 2.138 ± 0.349
1.742MetLeu: 1.742 ± 0.386
0.396MetMet: 0.396 ± 0.146
1.346MetAsn: 1.346 ± 0.301
0.554MetPro: 0.554 ± 0.219
0.871MetGln: 0.871 ± 0.245
0.95MetArg: 0.95 ± 0.271
1.504MetSer: 1.504 ± 0.322
1.979MetThr: 1.979 ± 0.371
0.713MetVal: 0.713 ± 0.252
0.317MetTrp: 0.317 ± 0.179
1.267MetTyr: 1.267 ± 0.337
0.0MetXaa: 0.0 ± 0.0
Asn
4.671AsnAla: 4.671 ± 0.669
0.475AsnCys: 0.475 ± 0.228
5.304AsnAsp: 5.304 ± 0.82
5.067AsnGlu: 5.067 ± 0.651
2.138AsnPhe: 2.138 ± 0.365
4.592AsnGly: 4.592 ± 0.668
1.108AsnHis: 1.108 ± 0.257
3.167AsnIle: 3.167 ± 0.464
6.334AsnLys: 6.334 ± 0.752
5.146AsnLeu: 5.146 ± 0.762
1.663AsnMet: 1.663 ± 0.334
5.146AsnAsn: 5.146 ± 1.013
2.454AsnPro: 2.454 ± 0.536
1.742AsnGln: 1.742 ± 0.389
2.058AsnArg: 2.058 ± 0.432
3.879AsnSer: 3.879 ± 0.633
3.879AsnThr: 3.879 ± 0.549
4.434AsnVal: 4.434 ± 0.652
0.95AsnTrp: 0.95 ± 0.292
3.088AsnTyr: 3.088 ± 0.705
0.0AsnXaa: 0.0 ± 0.0
Pro
1.504ProAla: 1.504 ± 0.379
0.158ProCys: 0.158 ± 0.108
1.267ProAsp: 1.267 ± 0.331
1.583ProGlu: 1.583 ± 0.417
1.346ProPhe: 1.346 ± 0.308
0.633ProGly: 0.633 ± 0.27
0.554ProHis: 0.554 ± 0.219
2.138ProIle: 2.138 ± 0.638
3.563ProLys: 3.563 ± 0.761
1.108ProLeu: 1.108 ± 0.283
0.713ProMet: 0.713 ± 0.217
2.058ProAsn: 2.058 ± 0.324
0.792ProPro: 0.792 ± 0.239
1.267ProGln: 1.267 ± 0.347
0.95ProArg: 0.95 ± 0.265
2.454ProSer: 2.454 ± 0.519
1.425ProThr: 1.425 ± 0.295
1.821ProVal: 1.821 ± 0.371
0.0ProTrp: 0.0 ± 0.0
0.792ProTyr: 0.792 ± 0.238
0.0ProXaa: 0.0 ± 0.0
Gln
3.008GlnAla: 3.008 ± 0.471
0.317GlnCys: 0.317 ± 0.148
1.821GlnAsp: 1.821 ± 0.355
2.375GlnGlu: 2.375 ± 0.532
1.583GlnPhe: 1.583 ± 0.349
1.425GlnGly: 1.425 ± 0.368
0.713GlnHis: 0.713 ± 0.24
2.454GlnIle: 2.454 ± 0.469
4.434GlnLys: 4.434 ± 0.604
4.117GlnLeu: 4.117 ± 0.651
0.713GlnMet: 0.713 ± 0.275
3.325GlnAsn: 3.325 ± 0.493
0.554GlnPro: 0.554 ± 0.225
1.821GlnGln: 1.821 ± 0.47
1.346GlnArg: 1.346 ± 0.335
2.138GlnSer: 2.138 ± 0.383
2.296GlnThr: 2.296 ± 0.365
3.088GlnVal: 3.088 ± 0.453
0.238GlnTrp: 0.238 ± 0.141
1.504GlnTyr: 1.504 ± 0.359
0.0GlnXaa: 0.0 ± 0.0
Arg
1.821ArgAla: 1.821 ± 0.364
0.238ArgCys: 0.238 ± 0.14
2.217ArgAsp: 2.217 ± 0.542
2.613ArgGlu: 2.613 ± 0.377
1.979ArgPhe: 1.979 ± 0.382
1.979ArgGly: 1.979 ± 0.455
0.95ArgHis: 0.95 ± 0.43
3.879ArgIle: 3.879 ± 0.478
3.167ArgLys: 3.167 ± 0.559
3.879ArgLeu: 3.879 ± 0.53
1.188ArgMet: 1.188 ± 0.272
1.9ArgAsn: 1.9 ± 0.31
0.871ArgPro: 0.871 ± 0.331
1.9ArgGln: 1.9 ± 0.402
1.029ArgArg: 1.029 ± 0.236
1.979ArgSer: 1.979 ± 0.478
2.217ArgThr: 2.217 ± 0.583
2.138ArgVal: 2.138 ± 0.366
0.554ArgTrp: 0.554 ± 0.197
2.375ArgTyr: 2.375 ± 0.394
0.0ArgXaa: 0.0 ± 0.0
Ser
3.088SerAla: 3.088 ± 0.585
0.0SerCys: 0.0 ± 0.0
3.563SerAsp: 3.563 ± 0.663
4.275SerGlu: 4.275 ± 0.523
2.454SerPhe: 2.454 ± 0.539
3.879SerGly: 3.879 ± 0.509
1.583SerHis: 1.583 ± 0.329
5.067SerIle: 5.067 ± 0.675
6.413SerLys: 6.413 ± 0.807
5.938SerLeu: 5.938 ± 0.604
1.504SerMet: 1.504 ± 0.357
4.275SerAsn: 4.275 ± 0.557
1.425SerPro: 1.425 ± 0.313
1.742SerGln: 1.742 ± 0.435
3.008SerArg: 3.008 ± 0.423
3.088SerSer: 3.088 ± 0.689
3.563SerThr: 3.563 ± 0.644
3.563SerVal: 3.563 ± 0.442
0.475SerTrp: 0.475 ± 0.251
2.296SerTyr: 2.296 ± 0.487
0.0SerXaa: 0.0 ± 0.0
Thr
3.088ThrAla: 3.088 ± 0.529
0.0ThrCys: 0.0 ± 0.0
4.038ThrAsp: 4.038 ± 0.472
3.879ThrGlu: 3.879 ± 0.64
2.217ThrPhe: 2.217 ± 0.454
3.088ThrGly: 3.088 ± 0.572
1.663ThrHis: 1.663 ± 0.278
4.988ThrIle: 4.988 ± 0.778
5.938ThrLys: 5.938 ± 0.763
5.146ThrLeu: 5.146 ± 0.636
1.029ThrMet: 1.029 ± 0.294
3.404ThrAsn: 3.404 ± 0.551
2.217ThrPro: 2.217 ± 0.366
2.217ThrGln: 2.217 ± 0.413
2.296ThrArg: 2.296 ± 0.425
3.8ThrSer: 3.8 ± 0.487
4.671ThrThr: 4.671 ± 0.813
4.275ThrVal: 4.275 ± 0.848
0.633ThrTrp: 0.633 ± 0.22
2.692ThrTyr: 2.692 ± 0.485
0.0ThrXaa: 0.0 ± 0.0
Val
4.038ValAla: 4.038 ± 0.639
0.238ValCys: 0.238 ± 0.131
4.513ValAsp: 4.513 ± 0.664
5.304ValGlu: 5.304 ± 0.752
2.929ValPhe: 2.929 ± 0.51
3.167ValGly: 3.167 ± 0.505
0.633ValHis: 0.633 ± 0.176
4.829ValIle: 4.829 ± 0.643
5.304ValLys: 5.304 ± 0.565
4.513ValLeu: 4.513 ± 0.596
1.504ValMet: 1.504 ± 0.344
4.829ValAsn: 4.829 ± 0.594
2.058ValPro: 2.058 ± 0.386
1.979ValGln: 1.979 ± 0.361
1.663ValArg: 1.663 ± 0.313
5.7ValSer: 5.7 ± 0.634
3.563ValThr: 3.563 ± 0.658
3.959ValVal: 3.959 ± 0.644
0.871ValTrp: 0.871 ± 0.236
2.613ValTyr: 2.613 ± 0.432
0.0ValXaa: 0.0 ± 0.0
Trp
1.029TrpAla: 1.029 ± 0.327
0.079TrpCys: 0.079 ± 0.076
0.633TrpAsp: 0.633 ± 0.198
0.396TrpGlu: 0.396 ± 0.166
0.396TrpPhe: 0.396 ± 0.168
0.396TrpGly: 0.396 ± 0.217
0.158TrpHis: 0.158 ± 0.116
0.396TrpIle: 0.396 ± 0.194
0.554TrpLys: 0.554 ± 0.227
0.871TrpLeu: 0.871 ± 0.234
0.158TrpMet: 0.158 ± 0.112
1.267TrpAsn: 1.267 ± 0.609
0.158TrpPro: 0.158 ± 0.128
0.396TrpGln: 0.396 ± 0.25
0.396TrpArg: 0.396 ± 0.157
1.108TrpSer: 1.108 ± 0.329
0.792TrpThr: 0.792 ± 0.26
0.871TrpVal: 0.871 ± 0.321
0.0TrpTrp: 0.0 ± 0.0
0.554TrpTyr: 0.554 ± 0.218
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.375TyrAla: 2.375 ± 0.425
0.238TyrCys: 0.238 ± 0.175
3.167TyrAsp: 3.167 ± 0.508
2.613TyrGlu: 2.613 ± 0.59
1.9TyrPhe: 1.9 ± 0.473
1.9TyrGly: 1.9 ± 0.366
1.029TyrHis: 1.029 ± 0.338
2.613TyrIle: 2.613 ± 0.541
5.067TyrLys: 5.067 ± 0.668
3.008TyrLeu: 3.008 ± 0.521
0.95TyrMet: 0.95 ± 0.339
2.85TyrAsn: 2.85 ± 0.483
1.188TyrPro: 1.188 ± 0.357
2.217TyrGln: 2.217 ± 0.491
2.138TyrArg: 2.138 ± 0.426
2.454TyrSer: 2.454 ± 0.421
2.375TyrThr: 2.375 ± 0.449
2.454TyrVal: 2.454 ± 0.413
0.871TyrTrp: 0.871 ± 0.309
1.9TyrTyr: 1.9 ± 0.47
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 70 proteins (12632 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski