Streptomyces adustus

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Actinobacteria; Actinomycetia; Streptomycetales; Streptomycetaceae; Streptomyces

Average proteome isoelectric point is 6.42

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 8617 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A5N8VB21|A0A5N8VB21_9ACTN Uncharacterized protein OS=Streptomyces adustus OX=1609272 GN=FNH09_12660 PE=4 SV=1
MM1 pKa = 7.51GLGGTPAAAAVACPSGVEE19 pKa = 4.06SDD21 pKa = 4.1FNGDD25 pKa = 4.28GIRR28 pKa = 11.84DD29 pKa = 3.57TAIADD34 pKa = 3.61PEE36 pKa = 4.22ATVDD40 pKa = 3.74GMAKK44 pKa = 10.07AGLVHH49 pKa = 6.17VVYY52 pKa = 10.68GGGKK56 pKa = 8.02GTLALSQATDD66 pKa = 5.16GIPGAPEE73 pKa = 3.69TGDD76 pKa = 3.12QYY78 pKa = 11.82GYY80 pKa = 11.22SLAVYY85 pKa = 10.16DD86 pKa = 5.51ADD88 pKa = 6.07LDD90 pKa = 4.13GCSDD94 pKa = 4.1LVVGSPYY101 pKa = 10.84EE102 pKa = 4.61DD103 pKa = 3.64IGTVPDD109 pKa = 3.6SGLVQVIYY117 pKa = 10.11GAPAGLGSGTKK128 pKa = 10.23AVTEE132 pKa = 4.19FLQGSDD138 pKa = 3.75KK139 pKa = 11.01PLGGTPEE146 pKa = 3.79TDD148 pKa = 2.69DD149 pKa = 3.27WVGYY153 pKa = 10.07AVAAGKK159 pKa = 8.55TSAGTPYY166 pKa = 11.06LLIGGPGEE174 pKa = 4.41SIGTVEE180 pKa = 4.86DD181 pKa = 3.96AGDD184 pKa = 4.11FYY186 pKa = 11.51YY187 pKa = 11.26VSGTALTVVGITQNTEE203 pKa = 3.35TGGAVPGVAEE213 pKa = 3.76QDD215 pKa = 3.46DD216 pKa = 4.42RR217 pKa = 11.84FGSTLAATPTHH228 pKa = 6.4FAVGTPGEE236 pKa = 4.23ALGTTTFAGGVAYY249 pKa = 10.12FSHH252 pKa = 7.04TLTSGYY258 pKa = 8.81PKK260 pKa = 10.33PLGGLGQDD268 pKa = 3.16QDD270 pKa = 4.42AISGAEE276 pKa = 3.85EE277 pKa = 3.87VGDD280 pKa = 3.76QFGAALAMVPYY291 pKa = 9.48RR292 pKa = 11.84AAGATSTTEE301 pKa = 3.61SLLAVGVPGEE311 pKa = 4.34DD312 pKa = 4.04LSTTVDD318 pKa = 3.14AGAVQVFRR326 pKa = 11.84LAANGTFTEE335 pKa = 4.77TAWIDD340 pKa = 3.51QNTADD345 pKa = 3.77VDD347 pKa = 4.0QEE349 pKa = 4.29AEE351 pKa = 3.86AGDD354 pKa = 3.79FFGRR358 pKa = 11.84RR359 pKa = 11.84LAAVNTSPNATSTGTNTRR377 pKa = 11.84LAIGVPGEE385 pKa = 4.37EE386 pKa = 4.09SSEE389 pKa = 4.11DD390 pKa = 3.48NPEE393 pKa = 3.83QGGVQIVPLVGAPGASDD410 pKa = 2.9QWLEE414 pKa = 4.19PGSGIPSGPAPRR426 pKa = 11.84MLTGLSLAATPSLLYY441 pKa = 10.68VGTPYY446 pKa = 11.0GPAAGQAVYY455 pKa = 10.4GFPWGVASGGAPTQTFKK472 pKa = 10.83PGEE475 pKa = 4.22GGIPATGVAFGTTVRR490 pKa = 4.25

Molecular weight: 48.5 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A5N8VFJ1|A0A5N8VFJ1_9ACTN RNA-binding transcriptional accessory protein OS=Streptomyces adustus OX=1609272 GN=FNH09_19800 PE=4 SV=1
MM1 pKa = 7.69SKK3 pKa = 9.0RR4 pKa = 11.84TFQPNNRR11 pKa = 11.84RR12 pKa = 11.84RR13 pKa = 11.84AKK15 pKa = 8.7THH17 pKa = 5.15GFRR20 pKa = 11.84LRR22 pKa = 11.84MRR24 pKa = 11.84TRR26 pKa = 11.84AGRR29 pKa = 11.84AILANRR35 pKa = 11.84RR36 pKa = 11.84SKK38 pKa = 10.89GRR40 pKa = 11.84ASLSAA45 pKa = 3.83

Molecular weight: 5.24 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

8617

0

8617

2795366

18

5576

324.4

34.73

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

13.595 ± 0.041

0.8 ± 0.007

6.057 ± 0.02

5.472 ± 0.031

2.722 ± 0.017

9.427 ± 0.028

2.345 ± 0.014

3.084 ± 0.019

2.084 ± 0.021

10.305 ± 0.036

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.69 ± 0.011

1.799 ± 0.015

6.002 ± 0.023

2.844 ± 0.015

8.058 ± 0.036

5.173 ± 0.025

6.331 ± 0.03

8.523 ± 0.024

1.547 ± 0.011

2.141 ± 0.014
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski