Pelagibacterium halotolerans (strain DSM 22347 / JCM 15775 / CGMCC 1.7692 / B2)

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Alphaproteobacteria; Hyphomicrobiales; Devosiaceae; Pelagibacterium; Pelagibacterium halotolerans

Average proteome isoelectric point is 6.18

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3875 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|G4RF18|G4RF18_PELHB Uncharacterized protein OS=Pelagibacterium halotolerans (strain DSM 22347 / JCM 15775 / CGMCC 1.7692 / B2) OX=1082931 GN=KKY_2956 PE=4 SV=1
MM1 pKa = 7.64LLLSSSTEE9 pKa = 3.88GFEE12 pKa = 4.4MKK14 pKa = 10.29RR15 pKa = 11.84LLLIGTAAYY24 pKa = 9.93AVLGSAAMAAEE35 pKa = 4.61PVTLWFWGAPPNLQDD50 pKa = 3.51AFEE53 pKa = 4.27EE54 pKa = 4.56VLVGPFNASQDD65 pKa = 3.61EE66 pKa = 4.52YY67 pKa = 10.92EE68 pKa = 4.23LQIEE72 pKa = 4.54FLQDD76 pKa = 2.36VDD78 pKa = 3.66NDD80 pKa = 3.11VRR82 pKa = 11.84TAVLAGEE89 pKa = 4.91GPDD92 pKa = 3.61LVYY95 pKa = 10.76TSGPSYY101 pKa = 10.43IAPLARR107 pKa = 11.84AGAIEE112 pKa = 4.48PLDD115 pKa = 4.29AYY117 pKa = 10.63AEE119 pKa = 4.2QYY121 pKa = 9.72GWHH124 pKa = 7.11DD125 pKa = 3.69RR126 pKa = 11.84LLEE129 pKa = 4.14PVLDD133 pKa = 3.53TCYY136 pKa = 10.7QLDD139 pKa = 4.17HH140 pKa = 7.15LYY142 pKa = 10.98CMPPALISDD151 pKa = 3.48GMFYY155 pKa = 11.03NRR157 pKa = 11.84ALLEE161 pKa = 4.22EE162 pKa = 4.92KK163 pKa = 10.09GWEE166 pKa = 4.29VPTTLAEE173 pKa = 4.22VEE175 pKa = 4.33QVMDD179 pKa = 3.98AAIADD184 pKa = 3.83GLYY187 pKa = 10.95ASVTGNKK194 pKa = 8.25GWQPVNEE201 pKa = 4.17NYY203 pKa = 11.05ASIFINNVVGPARR216 pKa = 11.84FYY218 pKa = 10.97EE219 pKa = 4.06ILSTGEE225 pKa = 4.1GWDD228 pKa = 3.39SAEE231 pKa = 3.92MIKK234 pKa = 10.51AIEE237 pKa = 4.02EE238 pKa = 3.87SARR241 pKa = 11.84WFKK244 pKa = 11.04AGYY247 pKa = 9.86LGGSDD252 pKa = 3.74YY253 pKa = 11.13FSLNFDD259 pKa = 3.52EE260 pKa = 5.85SISLVSQKK268 pKa = 10.37RR269 pKa = 11.84SPFFFAPSIGFQWATNYY286 pKa = 7.5FTGDD290 pKa = 3.26AAGDD294 pKa = 3.52FAFAPIPQMDD304 pKa = 3.96EE305 pKa = 3.9SLPYY309 pKa = 9.85PIYY312 pKa = 10.64DD313 pKa = 3.55LGVAFTLSINANSDD327 pKa = 3.58VKK329 pKa = 11.1DD330 pKa = 3.58GAAAVLDD337 pKa = 5.07LIFSPEE343 pKa = 3.98FASDD347 pKa = 3.71MADD350 pKa = 2.79VWPGYY355 pKa = 9.3WGIPLRR361 pKa = 11.84EE362 pKa = 4.23FPTNPDD368 pKa = 3.0ATGLTASFLDD378 pKa = 3.81AMADD382 pKa = 3.14MTAAVDD388 pKa = 3.26AGTFGFKK395 pKa = 9.87IGTFFPPATSQVMFEE410 pKa = 5.11DD411 pKa = 4.69IEE413 pKa = 4.95SVWLDD418 pKa = 3.08RR419 pKa = 11.84MTAQDD424 pKa = 4.21MLAKK428 pKa = 10.17AASAYY433 pKa = 9.9ADD435 pKa = 3.56EE436 pKa = 4.65MAQGLTQDD444 pKa = 4.54LPQPSMM450 pKa = 3.8

Molecular weight: 49.14 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|G4RGH2|G4RGH2_PELHB Uncharacterized protein OS=Pelagibacterium halotolerans (strain DSM 22347 / JCM 15775 / CGMCC 1.7692 / B2) OX=1082931 GN=KKY_101 PE=4 SV=1
MM1 pKa = 7.45KK2 pKa = 9.61RR3 pKa = 11.84TFQPSNLVRR12 pKa = 11.84ARR14 pKa = 11.84RR15 pKa = 11.84HH16 pKa = 4.37GFRR19 pKa = 11.84ARR21 pKa = 11.84MATKK25 pKa = 10.36NGRR28 pKa = 11.84KK29 pKa = 9.11IINRR33 pKa = 11.84RR34 pKa = 11.84RR35 pKa = 11.84AIGRR39 pKa = 11.84KK40 pKa = 9.12RR41 pKa = 11.84LSAA44 pKa = 3.98

Molecular weight: 5.23 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3875

0

3875

1168210

37

2379

301.5

32.67

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

12.203 ± 0.055

0.706 ± 0.012

5.879 ± 0.037

5.971 ± 0.041

3.914 ± 0.026

8.659 ± 0.044

1.941 ± 0.019

5.79 ± 0.03

2.908 ± 0.036

10.053 ± 0.042

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.65 ± 0.02

2.707 ± 0.023

4.993 ± 0.029

3.101 ± 0.026

6.517 ± 0.036

5.513 ± 0.03

5.481 ± 0.031

7.447 ± 0.035

1.29 ± 0.018

2.277 ± 0.018
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski