Methanoculleus bourgensis

Taxonomy: cellular organisms; Archaea;

Average proteome isoelectric point is 6.38

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3446 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A0X3BMZ6|A0A0X3BMZ6_9EURY Uncharacterized protein OS=Methanoculleus bourgensis OX=83986 GN=MMAB1_2148 PE=4 SV=1
MM1 pKa = 7.63LSLMLISVVSASYY14 pKa = 11.2PMFHH18 pKa = 7.28YY19 pKa = 7.87DD20 pKa = 3.43TQRR23 pKa = 11.84TGYY26 pKa = 9.55IPQDD30 pKa = 3.56GPQTNATLWVAEE42 pKa = 4.11TAEE45 pKa = 4.28YY46 pKa = 11.04ADD48 pKa = 4.53GSPAVYY54 pKa = 9.75NGKK57 pKa = 9.39VFVPTWPDD65 pKa = 2.48MDD67 pKa = 4.6FADD70 pKa = 4.91NDD72 pKa = 3.74PMGLVCYY79 pKa = 10.13DD80 pKa = 3.34AATGTEE86 pKa = 3.99LWTNEE91 pKa = 4.03LGGTSVGSVSGVAVADD107 pKa = 3.56GRR109 pKa = 11.84VYY111 pKa = 11.04LGGTDD116 pKa = 3.22GRR118 pKa = 11.84LYY120 pKa = 10.96CIDD123 pKa = 4.11EE124 pKa = 4.38EE125 pKa = 4.33TGEE128 pKa = 4.32MFWTSDD134 pKa = 3.39QIDD137 pKa = 3.46ATGYY141 pKa = 10.15FGLSSSPLIYY151 pKa = 10.57EE152 pKa = 3.83GTVYY156 pKa = 10.74VLSASDD162 pKa = 3.88GVLHH166 pKa = 7.2AFTPEE171 pKa = 4.66GIEE174 pKa = 3.9SWSFPTGGAVGYY186 pKa = 7.29FTSPAAANGEE196 pKa = 4.01IFVAGNEE203 pKa = 4.22SDD205 pKa = 4.42LSCIDD210 pKa = 3.3ITTHH214 pKa = 5.01TATWSVALPTAVKK227 pKa = 8.76STPAIGDD234 pKa = 3.33GKK236 pKa = 11.14VYY238 pKa = 9.58VTTAEE243 pKa = 3.82RR244 pKa = 11.84LYY246 pKa = 11.11ALSASTGAEE255 pKa = 3.7VWNASIGGTSSTPAVAGEE273 pKa = 4.1TVIAGSSDD281 pKa = 3.2GLHH284 pKa = 6.92AYY286 pKa = 9.6DD287 pKa = 5.18AGTGTPLWNFPSARR301 pKa = 11.84VDD303 pKa = 3.22VSPIIAGNLVYY314 pKa = 10.47AATNEE319 pKa = 4.34EE320 pKa = 4.25IGTVYY325 pKa = 10.74AVDD328 pKa = 3.26TGTGEE333 pKa = 4.42EE334 pKa = 4.29VWSYY338 pKa = 10.74TIEE341 pKa = 4.24APGDD345 pKa = 3.37GTFAAFFASSPAVSDD360 pKa = 3.53GVLYY364 pKa = 10.46IGVEE368 pKa = 4.05NNRR371 pKa = 11.84LYY373 pKa = 11.17AFGEE377 pKa = 4.74GSVSPTPTPTPTPTPAPGSWNGTVILAKK405 pKa = 9.54EE406 pKa = 4.44TFTFTPSNNASATYY420 pKa = 8.79TVNRR424 pKa = 11.84TTDD427 pKa = 3.49LGALTLAAQAGGFTINASDD446 pKa = 3.49AWYY449 pKa = 10.44ADD451 pKa = 3.35YY452 pKa = 11.19GSFMLEE458 pKa = 4.46DD459 pKa = 3.19IAGIANEE466 pKa = 4.9DD467 pKa = 3.25WTQEE471 pKa = 3.97NARR474 pKa = 11.84SWSIFINGAMAPAGLGANTLADD496 pKa = 3.82GDD498 pKa = 4.0RR499 pKa = 11.84LAFYY503 pKa = 9.66YY504 pKa = 10.65CPSDD508 pKa = 3.68PDD510 pKa = 3.68TYY512 pKa = 11.71APLIDD517 pKa = 3.44QAGYY521 pKa = 10.83VVTIDD526 pKa = 3.29VNVRR530 pKa = 11.84DD531 pKa = 4.58FTWEE535 pKa = 4.03GAVSLTDD542 pKa = 3.52GQTFTITPFNNEE554 pKa = 4.0SAIHH558 pKa = 5.12TFNRR562 pKa = 11.84TSALGALDD570 pKa = 3.82AAATAGGFNYY580 pKa = 9.48TVQEE584 pKa = 4.46TTWGPFLYY592 pKa = 10.46SIGGIAYY599 pKa = 10.29NEE601 pKa = 4.26TSWDD605 pKa = 3.08SWLYY609 pKa = 10.21SVNGIDD615 pKa = 4.45ASVGAADD622 pKa = 3.81YY623 pKa = 11.08QLTDD627 pKa = 3.82GDD629 pKa = 5.05VITYY633 pKa = 8.76WYY635 pKa = 9.08GAWGSTPDD643 pKa = 3.33TAGAVVDD650 pKa = 3.67ITVSIPATPAPTPTSGGGGGGGDD673 pKa = 3.87SPPSRR678 pKa = 11.84ITVTLQPGTFTITAEE693 pKa = 4.09NSGKK697 pKa = 9.38NHH699 pKa = 5.02TVSRR703 pKa = 11.84QTALGALDD711 pKa = 3.68ATGIAYY717 pKa = 8.52TIDD720 pKa = 3.16DD721 pKa = 4.42SYY723 pKa = 9.25YY724 pKa = 10.11QEE726 pKa = 4.39YY727 pKa = 11.0GSLFLSSIRR736 pKa = 11.84GRR738 pKa = 11.84VSEE741 pKa = 4.26GTRR744 pKa = 11.84GWMYY748 pKa = 10.47RR749 pKa = 11.84VNGGSPAVGANAYY762 pKa = 8.15PVTSGDD768 pKa = 3.48DD769 pKa = 3.82VIFFWSEE776 pKa = 4.09SMSSTPATSPDD787 pKa = 3.61VISIRR792 pKa = 11.84VVIPASSGSSGSDD805 pKa = 2.92SSGGGGSGSVPSSTTQEE822 pKa = 4.05QPNSVDD828 pKa = 3.09NSSASFLFGLPDD840 pKa = 3.61GAVIEE845 pKa = 4.3LGEE848 pKa = 4.05WGQTFSINTGPASAGEE864 pKa = 4.15EE865 pKa = 4.09VTISGNTFTINQSGIVLTIVARR887 pKa = 11.84NIDD890 pKa = 3.95EE891 pKa = 4.44KK892 pKa = 11.44DD893 pKa = 3.55GVASGLIEE901 pKa = 4.52SVTAAIDD908 pKa = 4.47LISGEE913 pKa = 4.03IEE915 pKa = 4.59GIGVVAASLDD925 pKa = 3.92LNLTGIPAADD935 pKa = 3.56GRR937 pKa = 11.84LDD939 pKa = 3.21ITFNATPDD947 pKa = 3.43ATAGNAFTLAATEE960 pKa = 3.88NDD962 pKa = 3.59EE963 pKa = 5.33EE964 pKa = 4.68IDD966 pKa = 3.5ALAYY970 pKa = 9.62TMTVTRR976 pKa = 11.84TNLEE980 pKa = 3.83NGEE983 pKa = 4.95DD984 pKa = 3.14ITGAVIRR991 pKa = 11.84MTINPEE997 pKa = 3.39WVEE1000 pKa = 3.72EE1001 pKa = 4.15HH1002 pKa = 6.59GGVDD1006 pKa = 3.24AVRR1009 pKa = 11.84IARR1012 pKa = 11.84SAEE1015 pKa = 3.92DD1016 pKa = 3.52STHH1019 pKa = 6.76EE1020 pKa = 4.36ILDD1023 pKa = 3.6TRR1025 pKa = 11.84LAGTDD1030 pKa = 3.39EE1031 pKa = 4.22NGNLIFEE1038 pKa = 4.54AVSPGGLSVFGLLTVRR1054 pKa = 11.84PASEE1058 pKa = 4.08MQEE1061 pKa = 4.41TPAVTATDD1069 pKa = 4.22PVSSTPVAAGTPEE1082 pKa = 4.19GAPPSLSSPFIGVGVGAALLIGAAYY1107 pKa = 10.23LIIRR1111 pKa = 11.84WRR1113 pKa = 11.84RR1114 pKa = 11.84EE1115 pKa = 3.43RR1116 pKa = 3.89

Molecular weight: 115.71 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A0X3BJQ0|A0A0X3BJQ0_9EURY UPF0285 protein MMAB1_0915 OS=Methanoculleus bourgensis OX=83986 GN=MMAB1_0915 PE=3 SV=1
MM1 pKa = 7.16VSGVNSFLARR11 pKa = 11.84QSSGGCTRR19 pKa = 11.84VTKK22 pKa = 10.86NIMVLTAAQSNIILYY37 pKa = 7.95NTIAHH42 pKa = 5.93NEE44 pKa = 4.02VRR46 pKa = 11.84SSARR50 pKa = 11.84PLKK53 pKa = 9.85WFVNARR59 pKa = 11.84AAGTLALPQPGRR71 pKa = 11.84LLFQRR76 pKa = 11.84RR77 pKa = 11.84EE78 pKa = 4.09FVDD81 pKa = 3.76EE82 pKa = 4.14VVPSSRR88 pKa = 11.84YY89 pKa = 9.78ARR91 pKa = 11.84FLRR94 pKa = 11.84SSPPIDD100 pKa = 3.44KK101 pKa = 9.21TATPDD106 pKa = 3.37MPGDD110 pKa = 3.6QASDD114 pKa = 3.57PRR116 pKa = 11.84LGTAGSIARR125 pKa = 11.84CDD127 pKa = 3.8TIAVDD132 pKa = 4.14LRR134 pKa = 11.84RR135 pKa = 11.84HH136 pKa = 5.73EE137 pKa = 4.49GRR139 pKa = 11.84PCAGWKK145 pKa = 9.6

Molecular weight: 15.84 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3446

0

3446

822921

13

2024

238.8

26.26

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

9.62 ± 0.059

1.352 ± 0.022

5.658 ± 0.04

6.787 ± 0.049

3.538 ± 0.028

8.471 ± 0.044

2.025 ± 0.022

6.218 ± 0.037

3.402 ± 0.039

9.219 ± 0.051

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.446 ± 0.025

2.767 ± 0.033

5.259 ± 0.034

2.537 ± 0.024

7.364 ± 0.066

5.569 ± 0.046

5.702 ± 0.058

7.896 ± 0.039

1.141 ± 0.018

3.028 ± 0.03
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski