Amino acid dipepetide frequency for Methanoculleus bourgensis

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10^-3.

For more information see sequence space article on Wikipedia.

	Ala	Cys	Asp	Glu	Phe	Gly	His	Ile	Lys	Leu	Met	Asn	Pro	Gln	Arg	Ser	Thr	Val	Trp	Tyr	Xaa
Ala	10.927AlaAla: 10.927 ± 0.164	1.298AlaCys: 1.298 ± 0.042	5.25AlaAsp: 5.25 ± 0.094	5.968AlaGlu: 5.968 ± 0.087	3.472AlaPhe: 3.472 ± 0.072	9.651AlaGly: 9.651 ± 0.14	1.637AlaHis: 1.637 ± 0.043	5.789AlaIle: 5.789 ± 0.091	2.938AlaLys: 2.938 ± 0.062	9.737AlaLeu: 9.737 ± 0.148	2.532AlaMet: 2.532 ± 0.062	2.138AlaAsn: 2.138 ± 0.059	4.136AlaPro: 4.136 ± 0.076	1.819AlaGln: 1.819 ± 0.05	6.784AlaArg: 6.784 ± 0.108	4.969AlaSer: 4.969 ± 0.074	4.992AlaThr: 4.992 ± 0.101	8.51AlaVal: 8.51 ± 0.119	1.164AlaTrp: 1.164 ± 0.041	2.485AlaTyr: 2.485 ± 0.057	0.0AlaXaa: 0.0 ± 0.0
Cys	1.062CysAla: 1.062 ± 0.039	0.277CysCys: 0.277 ± 0.019	0.756CysAsp: 0.756 ± 0.029	0.653CysGlu: 0.653 ± 0.034	0.386CysPhe: 0.386 ± 0.021	1.547CysGly: 1.547 ± 0.05	0.334CysHis: 0.334 ± 0.024	0.86CysIle: 0.86 ± 0.034	0.317CysLys: 0.317 ± 0.021	1.057CysLeu: 1.057 ± 0.039	0.338CysMet: 0.338 ± 0.021	0.36CysAsn: 0.36 ± 0.022	0.939CysPro: 0.939 ± 0.045	0.247CysGln: 0.247 ± 0.019	1.238CysArg: 1.238 ± 0.046	0.904CysSer: 0.904 ± 0.039	0.902CysThr: 0.902 ± 0.035	0.753CysVal: 0.753 ± 0.033	0.149CysTrp: 0.149 ± 0.016	0.437CysTyr: 0.437 ± 0.025	0.0CysXaa: 0.0 ± 0.0
Asp	5.826AspAla: 5.826 ± 0.088	0.677AspCys: 0.677 ± 0.029	3.204AspAsp: 3.204 ± 0.078	4.054AspGlu: 4.054 ± 0.08	1.715AspPhe: 1.715 ± 0.044	4.582AspGly: 4.582 ± 0.093	1.179AspHis: 1.179 ± 0.034	3.85AspIle: 3.85 ± 0.068	1.216AspLys: 1.216 ± 0.046	6.838AspLeu: 6.838 ± 0.104	1.218AspMet: 1.218 ± 0.037	1.185AspAsn: 1.185 ± 0.049	3.596AspPro: 3.596 ± 0.073	1.335AspGln: 1.335 ± 0.044	4.361AspArg: 4.361 ± 0.088	2.134AspSer: 2.134 ± 0.063	2.887AspThr: 2.887 ± 0.07	4.26AspVal: 4.26 ± 0.077	0.574AspTrp: 0.574 ± 0.03	1.89AspTyr: 1.89 ± 0.047	0.0AspXaa: 0.0 ± 0.0
Glu	6.251GluAla: 6.251 ± 0.102	0.736GluCys: 0.736 ± 0.029	3.598GluAsp: 3.598 ± 0.067	5.9GluGlu: 5.9 ± 0.123	2.277GluPhe: 2.277 ± 0.056	5.042GluGly: 5.042 ± 0.086	1.529GluHis: 1.529 ± 0.053	4.663GluIle: 4.663 ± 0.092	3.133GluLys: 3.133 ± 0.066	4.845GluLeu: 4.845 ± 0.088	1.811GluMet: 1.811 ± 0.049	2.237GluAsn: 2.237 ± 0.05	2.989GluPro: 2.989 ± 0.065	2.045GluGln: 2.045 ± 0.048	5.519GluArg: 5.519 ± 0.096	3.105GluSer: 3.105 ± 0.064	3.804GluThr: 3.804 ± 0.08	5.612GluVal: 5.612 ± 0.088	0.702GluTrp: 0.702 ± 0.033	2.069GluTyr: 2.069 ± 0.047	0.0GluXaa: 0.0 ± 0.0
Phe	3.535PheAla: 3.535 ± 0.073	0.561PheCys: 0.561 ± 0.027	2.18PheAsp: 2.18 ± 0.061	2.212PheGlu: 2.212 ± 0.049	1.506PhePhe: 1.506 ± 0.046	3.09PheGly: 3.09 ± 0.07	0.707PheHis: 0.707 ± 0.03	2.089PheIle: 2.089 ± 0.05	0.866PheLys: 0.866 ± 0.035	3.298PheLeu: 3.298 ± 0.083	0.722PheMet: 0.722 ± 0.031	0.978PheAsn: 0.978 ± 0.038	1.617PhePro: 1.617 ± 0.042	0.859PheGln: 0.859 ± 0.037	2.328PheArg: 2.328 ± 0.048	2.676PheSer: 2.676 ± 0.067	2.091PheThr: 2.091 ± 0.054	2.608PheVal: 2.608 ± 0.05	0.42PheTrp: 0.42 ± 0.023	1.04PheTyr: 1.04 ± 0.044	0.0PheXaa: 0.0 ± 0.0
Gly	7.41GlyAla: 7.41 ± 0.116	1.361GlyCys: 1.361 ± 0.046	4.923GlyAsp: 4.923 ± 0.099	5.975GlyGlu: 5.975 ± 0.092	2.952GlyPhe: 2.952 ± 0.072	6.916GlyGly: 6.916 ± 0.115	1.532GlyHis: 1.532 ± 0.045	5.957GlyIle: 5.957 ± 0.097	3.56GlyLys: 3.56 ± 0.065	6.857GlyLeu: 6.857 ± 0.119	2.559GlyMet: 2.559 ± 0.061	2.522GlyAsn: 2.522 ± 0.075	2.989GlyPro: 2.989 ± 0.072	1.796GlyGln: 1.796 ± 0.047	6.3GlyArg: 6.3 ± 0.11	5.211GlySer: 5.211 ± 0.092	5.457GlyThr: 5.457 ± 0.107	6.533GlyVal: 6.533 ± 0.099	1.068GlyTrp: 1.068 ± 0.04	2.834GlyTyr: 2.834 ± 0.064	0.0GlyXaa: 0.0 ± 0.0
His	1.786HisAla: 1.786 ± 0.047	0.344HisCys: 0.344 ± 0.023	1.146HisAsp: 1.146 ± 0.035	1.112HisGlu: 1.112 ± 0.039	0.688HisPhe: 0.688 ± 0.026	1.671HisGly: 1.671 ± 0.045	0.605HisHis: 0.605 ± 0.028	1.219HisIle: 1.219 ± 0.037	0.42HisLys: 0.42 ± 0.023	2.241HisLeu: 2.241 ± 0.058	0.406HisMet: 0.406 ± 0.021	0.547HisAsn: 0.547 ± 0.026	1.571HisPro: 1.571 ± 0.046	0.621HisGln: 0.621 ± 0.026	1.609HisArg: 1.609 ± 0.049	0.939HisSer: 0.939 ± 0.039	1.113HisThr: 1.113 ± 0.038	1.329HisVal: 1.329 ± 0.044	0.23HisTrp: 0.23 ± 0.018	0.655HisTyr: 0.655 ± 0.029	0.0HisXaa: 0.0 ± 0.0
Ile	6.623IleAla: 6.623 ± 0.098	0.832IleCys: 0.832 ± 0.034	3.856IleAsp: 3.856 ± 0.085	4.293IleGlu: 4.293 ± 0.088	2.197IlePhe: 2.197 ± 0.058	5.069IleGly: 5.069 ± 0.094	1.198IleHis: 1.198 ± 0.037	3.537IleIle: 3.537 ± 0.071	1.764IleLys: 1.764 ± 0.053	5.732IleLeu: 5.732 ± 0.101	1.17IleMet: 1.17 ± 0.043	1.721IleAsn: 1.721 ± 0.048	3.587IlePro: 3.587 ± 0.063	1.571IleGln: 1.571 ± 0.049	4.632IleArg: 4.632 ± 0.075	3.547IleSer: 3.547 ± 0.075	3.907IleThr: 3.907 ± 0.079	4.533IleVal: 4.533 ± 0.09	0.58IleTrp: 0.58 ± 0.025	1.831IleTyr: 1.831 ± 0.055	0.0IleXaa: 0.0 ± 0.0
Lys	2.807LysAla: 2.807 ± 0.064	0.388LysCys: 0.388 ± 0.024	1.958LysAsp: 1.958 ± 0.052	2.683LysGlu: 2.683 ± 0.063	0.994LysPhe: 0.994 ± 0.05	2.462LysGly: 2.462 ± 0.064	0.615LysHis: 0.615 ± 0.028	2.285LysIle: 2.285 ± 0.064	2.103LysLys: 2.103 ± 0.06	2.303LysLeu: 2.303 ± 0.074	0.875LysMet: 0.875 ± 0.035	1.329LysAsn: 1.329 ± 0.04	1.716LysPro: 1.716 ± 0.045	1.035LysGln: 1.035 ± 0.041	2.491LysArg: 2.491 ± 0.058	1.792LysSer: 1.792 ± 0.051	2.191LysThr: 2.191 ± 0.055	2.566LysVal: 2.566 ± 0.059	0.375LysTrp: 0.375 ± 0.027	1.054LysTyr: 1.054 ± 0.039	0.0LysXaa: 0.0 ± 0.0
Leu	10.0LeuAla: 10.0 ± 0.127	1.112LeuCys: 1.112 ± 0.042	5.438LeuAsp: 5.438 ± 0.093	5.686LeuGlu: 5.686 ± 0.085	3.604LeuPhe: 3.604 ± 0.08	6.775LeuGly: 6.775 ± 0.111	1.813LeuHis: 1.813 ± 0.052	5.841LeuIle: 5.841 ± 0.111	3.235LeuLys: 3.235 ± 0.071	9.273LeuLeu: 9.273 ± 0.138	2.099LeuMet: 2.099 ± 0.048	2.43LeuAsn: 2.43 ± 0.052	4.949LeuPro: 4.949 ± 0.087	2.726LeuGln: 2.726 ± 0.071	6.238LeuArg: 6.238 ± 0.094	5.59LeuSer: 5.59 ± 0.092	4.898LeuThr: 4.898 ± 0.08	6.913LeuVal: 6.913 ± 0.098	0.937LeuTrp: 0.937 ± 0.036	2.637LeuTyr: 2.637 ± 0.059	0.0LeuXaa: 0.0 ± 0.0
Met	2.229MetAla: 2.229 ± 0.049	0.225MetCys: 0.225 ± 0.018	1.264MetAsp: 1.264 ± 0.038	1.673MetGlu: 1.673 ± 0.044	0.575MetPhe: 0.575 ± 0.028	1.871MetGly: 1.871 ± 0.054	0.521MetHis: 0.521 ± 0.027	1.45MetIle: 1.45 ± 0.041	1.266MetLys: 1.266 ± 0.036	2.289MetLeu: 2.289 ± 0.059	0.69MetMet: 0.69 ± 0.033	0.898MetAsn: 0.898 ± 0.032	1.271MetPro: 1.271 ± 0.042	0.847MetGln: 0.847 ± 0.035	1.865MetArg: 1.865 ± 0.051	1.261MetSer: 1.261 ± 0.036	1.562MetThr: 1.562 ± 0.05	1.989MetVal: 1.989 ± 0.053	0.193MetTrp: 0.193 ± 0.016	0.519MetTyr: 0.519 ± 0.027	0.0MetXaa: 0.0 ± 0.0
Asn	2.489AsnAla: 2.489 ± 0.054	0.397AsnCys: 0.397 ± 0.028	1.371AsnAsp: 1.371 ± 0.047	1.65AsnGlu: 1.65 ± 0.051	0.894AsnPhe: 0.894 ± 0.032	2.164AsnGly: 2.164 ± 0.063	0.56AsnHis: 0.56 ± 0.026	1.797AsnIle: 1.797 ± 0.051	0.82AsnLys: 0.82 ± 0.038	2.972AsnLeu: 2.972 ± 0.07	0.516AsnMet: 0.516 ± 0.023	0.857AsnAsn: 0.857 ± 0.035	2.08AsnPro: 2.08 ± 0.055	0.774AsnGln: 0.774 ± 0.039	2.06AsnArg: 2.06 ± 0.053	1.292AsnSer: 1.292 ± 0.047	1.538AsnThr: 1.538 ± 0.045	2.209AsnVal: 2.209 ± 0.066	0.309AsnTrp: 0.309 ± 0.019	0.915AsnTyr: 0.915 ± 0.034	0.0AsnXaa: 0.0 ± 0.0
Pro	5.301ProAla: 5.301 ± 0.102	0.784ProCys: 0.784 ± 0.037	3.716ProAsp: 3.716 ± 0.076	4.482ProGlu: 4.482 ± 0.086	1.86ProPhe: 1.86 ± 0.052	5.867ProGly: 5.867 ± 0.096	1.105ProHis: 1.105 ± 0.035	2.269ProIle: 2.269 ± 0.053	1.436ProLys: 1.436 ± 0.04	4.437ProLeu: 4.437 ± 0.082	1.134ProMet: 1.134 ± 0.037	1.088ProAsn: 1.088 ± 0.036	2.966ProPro: 2.966 ± 0.072	1.151ProGln: 1.151 ± 0.04	3.005ProArg: 3.005 ± 0.07	2.636ProSer: 2.636 ± 0.068	2.593ProThr: 2.593 ± 0.084	4.715ProVal: 4.715 ± 0.076	0.576ProTrp: 0.576 ± 0.027	1.473ProTyr: 1.473 ± 0.037	0.0ProXaa: 0.0 ± 0.0
Gln	2.299GlnAla: 2.299 ± 0.054	0.277GlnCys: 0.277 ± 0.019	1.328GlnAsp: 1.328 ± 0.038	1.95GlnGlu: 1.95 ± 0.052	0.899GlnPhe: 0.899 ± 0.034	1.928GlnGly: 1.928 ± 0.052	0.574GlnHis: 0.574 ± 0.026	1.685GlnIle: 1.685 ± 0.052	1.384GlnLys: 1.384 ± 0.046	1.541GlnLeu: 1.541 ± 0.047	0.759GlnMet: 0.759 ± 0.027	0.898GlnAsn: 0.898 ± 0.038	1.084GlnPro: 1.084 ± 0.037	0.956GlnGln: 0.956 ± 0.041	1.896GlnArg: 1.896 ± 0.051	1.293GlnSer: 1.293 ± 0.037	1.309GlnThr: 1.309 ± 0.04	2.131GlnVal: 2.131 ± 0.053	0.375GlnTrp: 0.375 ± 0.025	0.801GlnTyr: 0.801 ± 0.029	0.0GlnXaa: 0.0 ± 0.0
Arg	6.179ArgAla: 6.179 ± 0.101	1.113ArgCys: 1.113 ± 0.04	4.269ArgAsp: 4.269 ± 0.075	6.086ArgGlu: 6.086 ± 0.096	2.693ArgPhe: 2.693 ± 0.071	5.525ArgGly: 5.525 ± 0.095	1.619ArgHis: 1.619 ± 0.05	4.468ArgIle: 4.468 ± 0.077	2.83ArgLys: 2.83 ± 0.066	6.262ArgLeu: 6.262 ± 0.109	2.236ArgMet: 2.236 ± 0.051	2.043ArgAsn: 2.043 ± 0.053	3.432ArgPro: 3.432 ± 0.075	2.04ArgGln: 2.04 ± 0.044	6.022ArgArg: 6.022 ± 0.107	4.301ArgSer: 4.301 ± 0.087	3.522ArgThr: 3.522 ± 0.065	5.399ArgVal: 5.399 ± 0.098	0.949ArgTrp: 0.949 ± 0.034	2.656ArgTyr: 2.656 ± 0.065	0.0ArgXaa: 0.0 ± 0.0
Ser	4.862SerAla: 4.862 ± 0.087	0.69SerCys: 0.69 ± 0.028	2.824SerAsp: 2.824 ± 0.065	2.898SerGlu: 2.898 ± 0.06	1.953SerPhe: 1.953 ± 0.046	6.008SerGly: 6.008 ± 0.103	1.203SerHis: 1.203 ± 0.041	3.373SerIle: 3.373 ± 0.059	1.424SerLys: 1.424 ± 0.047	5.131SerLeu: 5.131 ± 0.08	1.373SerMet: 1.373 ± 0.041	1.297SerAsn: 1.297 ± 0.046	3.597SerPro: 3.597 ± 0.074	1.1SerGln: 1.1 ± 0.038	4.615SerArg: 4.615 ± 0.072	3.904SerSer: 3.904 ± 0.102	3.198SerThr: 3.198 ± 0.081	4.101SerVal: 4.101 ± 0.069	0.678SerTrp: 0.678 ± 0.033	1.457SerTyr: 1.457 ± 0.048	0.0SerXaa: 0.0 ± 0.0
Thr	5.428ThrAla: 5.428 ± 0.11	0.722ThrCys: 0.722 ± 0.031	2.864ThrAsp: 2.864 ± 0.066	3.082ThrGlu: 3.082 ± 0.068	2.196ThrPhe: 2.196 ± 0.062	5.717ThrGly: 5.717 ± 0.097	1.034ThrHis: 1.034 ± 0.041	3.71ThrIle: 3.71 ± 0.068	1.568ThrLys: 1.568 ± 0.051	5.559ThrLeu: 5.559 ± 0.078	1.232ThrMet: 1.232 ± 0.045	1.43ThrAsn: 1.43 ± 0.049	3.729ThrPro: 3.729 ± 0.09	1.052ThrGln: 1.052 ± 0.038	3.394ThrArg: 3.394 ± 0.07	3.089ThrSer: 3.089 ± 0.062	3.451ThrThr: 3.451 ± 0.092	5.219ThrVal: 5.219 ± 0.101	0.746ThrTrp: 0.746 ± 0.042	1.792ThrTyr: 1.792 ± 0.058	0.0ThrXaa: 0.0 ± 0.0
Val	7.631ValAla: 7.631 ± 0.107	1.123ValCys: 1.123 ± 0.04	4.297ValAsp: 4.297 ± 0.068	5.036ValGlu: 5.036 ± 0.089	3.044ValPhe: 3.044 ± 0.062	5.405ValGly: 5.405 ± 0.088	1.534ValHis: 1.534 ± 0.051	4.836ValIle: 4.836 ± 0.079	2.506ValLys: 2.506 ± 0.057	7.656ValLeu: 7.656 ± 0.11	1.897ValMet: 1.897 ± 0.046	2.264ValAsn: 2.264 ± 0.064	4.276ValPro: 4.276 ± 0.073	2.065ValGln: 2.065 ± 0.055	5.884ValArg: 5.884 ± 0.089	4.616ValSer: 4.616 ± 0.08	5.083ValThr: 5.083 ± 0.116	6.713ValVal: 6.713 ± 0.104	0.83ValTrp: 0.83 ± 0.044	2.269ValTyr: 2.269 ± 0.064	0.0ValXaa: 0.0 ± 0.0
Trp	0.811TrpAla: 0.811 ± 0.036	0.181TrpCys: 0.181 ± 0.015	0.608TrpAsp: 0.608 ± 0.032	0.733TrpGlu: 0.733 ± 0.033	0.383TrpPhe: 0.383 ± 0.023	0.891TrpGly: 0.891 ± 0.038	0.248TrpHis: 0.248 ± 0.017	0.66TrpIle: 0.66 ± 0.034	0.518TrpLys: 0.518 ± 0.026	1.045TrpLeu: 1.045 ± 0.035	0.341TrpMet: 0.341 ± 0.018	0.48TrpAsn: 0.48 ± 0.025	0.371TrpPro: 0.371 ± 0.024	0.368TrpGln: 0.368 ± 0.022	0.886TrpArg: 0.886 ± 0.034	0.825TrpSer: 0.825 ± 0.041	0.62TrpThr: 0.62 ± 0.032	0.847TrpVal: 0.847 ± 0.035	0.205TrpTrp: 0.205 ± 0.017	0.395TrpTyr: 0.395 ± 0.022	0.0TrpXaa: 0.0 ± 0.0
Tyr	2.743TyrAla: 2.743 ± 0.061	0.419TyrCys: 0.419 ± 0.022	1.732TyrAsp: 1.732 ± 0.048	1.743TyrGlu: 1.743 ± 0.048	1.097TyrPhe: 1.097 ± 0.039	2.53TyrGly: 2.53 ± 0.066	0.706TyrHis: 0.706 ± 0.03	1.842TyrIle: 1.842 ± 0.051	0.716TyrLys: 0.716 ± 0.028	3.121TyrLeu: 3.121 ± 0.071	0.549TyrMet: 0.549 ± 0.027	0.966TyrAsn: 0.966 ± 0.043	1.694TyrPro: 1.694 ± 0.05	0.961TyrGln: 0.961 ± 0.035	2.515TyrArg: 2.515 ± 0.057	1.603TyrSer: 1.603 ± 0.041	1.898TyrThr: 1.898 ± 0.06	2.022TyrVal: 2.022 ± 0.055	0.352TyrTrp: 0.352 ± 0.018	1.071TyrTyr: 1.071 ± 0.037	0.0TyrXaa: 0.0 ± 0.0
Xaa	0.0XaaAla: 0.0 ± 0.0	0.0XaaCys: 0.0 ± 0.0	0.0XaaAsp: 0.0 ± 0.0	0.0XaaGlu: 0.0 ± 0.0	0.0XaaPhe: 0.0 ± 0.0	0.0XaaGly: 0.0 ± 0.0	0.0XaaHis: 0.0 ± 0.0	0.0XaaIle: 0.0 ± 0.0	0.0XaaLys: 0.0 ± 0.0	0.0XaaLeu: 0.0 ± 0.0	0.0XaaMet: 0.0 ± 0.0	0.0XaaAsn: 0.0 ± 0.0	0.0XaaPro: 0.0 ± 0.0	0.0XaaGln: 0.0 ± 0.0	0.0XaaArg: 0.0 ± 0.0	0.0XaaSer: 0.0 ± 0.0	0.0XaaThr: 0.0 ± 0.0	0.0XaaVal: 0.0 ± 0.0	0.0XaaTrp: 0.0 ± 0.0	0.0XaaTyr: 0.0 ± 0.0	0.0XaaXaa: 0.0 ± 0.0

Statistics based on 3446 proteins (822921 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944

Contact: Lukasz P. Kozlowski

---