Rhinolophus ferrumequinum papillomavirus 1
Average proteome isoelectric point is 5.99
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 6 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|W8EH64|W8EH64_9PAPI Protein E7 OS=Rhinolophus ferrumequinum papillomavirus 1 OX=1464074 GN=E7 PE=3 SV=1
MM1 pKa = 7.64 RR2 pKa = 11.84 GSHH5 pKa = 6.57 IDD7 pKa = 3.36 LRR9 pKa = 11.84 DD10 pKa = 3.71 IILQEE15 pKa = 3.95 EE16 pKa = 4.81 PEE18 pKa = 4.59 NIDD21 pKa = 3.77 LRR23 pKa = 11.84 CDD25 pKa = 3.55 EE26 pKa = 4.38 ILQEE30 pKa = 4.28 EE31 pKa = 4.77 EE32 pKa = 4.68 EE33 pKa = 4.33 EE34 pKa = 4.32 QQVHH38 pKa = 5.64 GLISRR43 pKa = 11.84 QAFQVAICCGLCQRR57 pKa = 11.84 PIKK60 pKa = 9.62 FVCLTTILALRR71 pKa = 11.84 QLEE74 pKa = 4.03 QLLFGPLDD82 pKa = 3.96 FVCVRR87 pKa = 11.84 CVNTHH92 pKa = 5.62 EE93 pKa = 4.25 LHH95 pKa = 7.14 HH96 pKa = 6.64 GGG98 pKa = 4.14
Molecular weight: 11.27 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 4.774
IPC2_protein 4.927
IPC_protein 4.813
Toseland 4.749
ProMoST 4.94
Dawson 4.787
Bjellqvist 4.927
Wikipedia 4.635
Rodwell 4.711
Grimsley 4.673
Solomon 4.787
Lehninger 4.736
Nozaki 4.914
DTASelect 5.016
Thurlkill 4.749
EMBOSS 4.685
Sillero 4.991
Patrickios 3.325
IPC_peptide 4.8
IPC2_peptide 4.991
IPC2.peptide.svr19 4.9
Protein with the highest isoelectric point:
>tr|W8EG07|W8EG07_9PAPI Replication protein E1 OS=Rhinolophus ferrumequinum papillomavirus 1 OX=1464074 GN=E1 PE=3 SV=1
MM1 pKa = 7.06 EE2 pKa = 4.73 TLSSRR7 pKa = 11.84 LEE9 pKa = 4.21 SVQDD13 pKa = 3.75 ALLTLYY19 pKa = 10.25 EE20 pKa = 4.04 QDD22 pKa = 3.89 SNRR25 pKa = 11.84 LDD27 pKa = 4.03 DD28 pKa = 6.08 QIKK31 pKa = 9.27 QWTLVKK37 pKa = 10.55 EE38 pKa = 4.4 EE39 pKa = 4.12 NVLLHH44 pKa = 6.81 AARR47 pKa = 11.84 KK48 pKa = 9.77 KK49 pKa = 10.7 GITRR53 pKa = 11.84 LGMHH57 pKa = 6.62 MVPTLQSSEE66 pKa = 3.55 ARR68 pKa = 11.84 ARR70 pKa = 11.84 DD71 pKa = 3.99 AIEE74 pKa = 3.74 MEE76 pKa = 5.03 LYY78 pKa = 10.57 LKK80 pKa = 10.02 SLRR83 pKa = 11.84 QSPYY87 pKa = 9.19 GTEE90 pKa = 3.86 PWNLAQTTRR99 pKa = 11.84 EE100 pKa = 4.04 RR101 pKa = 11.84 FLAAPPYY108 pKa = 9.95 CFKK111 pKa = 11.01 KK112 pKa = 10.6 DD113 pKa = 3.48 GSNVDD118 pKa = 3.19 VYY120 pKa = 11.83 YY121 pKa = 11.1 DD122 pKa = 3.59 NDD124 pKa = 3.43 KK125 pKa = 11.3 DD126 pKa = 3.72 NSVRR130 pKa = 11.84 YY131 pKa = 7.88 ITWGAIYY138 pKa = 9.38 YY139 pKa = 10.46 QNGNDD144 pKa = 4.03 DD145 pKa = 3.13 WHH147 pKa = 6.43 KK148 pKa = 8.04 VQGRR152 pKa = 11.84 IEE154 pKa = 4.42 HH155 pKa = 7.01 DD156 pKa = 2.96 GLYY159 pKa = 10.06 YY160 pKa = 10.21 VQHH163 pKa = 7.46 DD164 pKa = 4.43 GLKK167 pKa = 8.52 VTYY170 pKa = 10.66 VDD172 pKa = 5.29 FAAEE176 pKa = 3.7 ASAYY180 pKa = 10.52 SRR182 pKa = 11.84 TGSWRR187 pKa = 11.84 VVYY190 pKa = 10.47 NNKK193 pKa = 8.32 TVLPDD198 pKa = 3.29 NSVASSRR205 pKa = 11.84 SGRR208 pKa = 11.84 QRR210 pKa = 11.84 TSSPTGPGAEE220 pKa = 3.95 YY221 pKa = 10.46 SPRR224 pKa = 11.84 STTPTDD230 pKa = 3.47 TVVATPLKK238 pKa = 10.52 ASKK241 pKa = 10.3 KK242 pKa = 8.44 DD243 pKa = 3.13 TAAGVRR249 pKa = 11.84 RR250 pKa = 11.84 GTRR253 pKa = 11.84 HH254 pKa = 5.78 HH255 pKa = 6.7 NGAGRR260 pKa = 11.84 RR261 pKa = 11.84 GGGRR265 pKa = 11.84 RR266 pKa = 11.84 VQQRR270 pKa = 11.84 KK271 pKa = 9.42 SDD273 pKa = 3.97 PKK275 pKa = 9.98 RR276 pKa = 11.84 GAAITPPSPSEE287 pKa = 3.61 VGGRR291 pKa = 11.84 HH292 pKa = 4.37 QTPQRR297 pKa = 11.84 RR298 pKa = 11.84 SGSRR302 pKa = 11.84 LQRR305 pKa = 11.84 LLQEE309 pKa = 4.8 ARR311 pKa = 11.84 DD312 pKa = 3.97 PPAVLLKK319 pKa = 10.81 GQANTLKK326 pKa = 10.56 CFRR329 pKa = 11.84 FQAKK333 pKa = 10.07 AKK335 pKa = 10.06 FAGQFRR341 pKa = 11.84 SVSTTFYY348 pKa = 7.95 WTASEE353 pKa = 4.2 GTDD356 pKa = 2.73 RR357 pKa = 11.84 VGRR360 pKa = 11.84 ARR362 pKa = 11.84 VIFLFTDD369 pKa = 3.24 KK370 pKa = 10.95 GQRR373 pKa = 11.84 EE374 pKa = 4.11 RR375 pKa = 11.84 FMSRR379 pKa = 11.84 VKK381 pKa = 10.38 FPPSIEE387 pKa = 4.2 TVLLNMDD394 pKa = 4.28 GFF396 pKa = 4.35
Molecular weight: 44.63 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.219
IPC2_protein 9.385
IPC_protein 9.604
Toseland 10.028
ProMoST 9.765
Dawson 10.248
Bjellqvist 9.94
Wikipedia 10.438
Rodwell 10.54
Grimsley 10.335
Solomon 10.277
Lehninger 10.248
Nozaki 10.014
DTASelect 9.94
Thurlkill 10.101
EMBOSS 10.438
Sillero 10.16
Patrickios 9.897
IPC_peptide 10.277
IPC2_peptide 8.624
IPC2.peptide.svr19 8.472
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
6
0
6
2282
98
610
380.3
42.74
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
6.179 ± 0.429
2.016 ± 0.758
6.31 ± 0.262
6.486 ± 0.646
4.689 ± 0.333
6.573 ± 0.665
2.235 ± 0.168
4.251 ± 0.558
4.514 ± 0.864
8.282 ± 0.802
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
1.972 ± 0.458
3.769 ± 0.586
6.223 ± 0.899
4.514 ± 0.417
6.836 ± 0.851
7.406 ± 0.7
6.398 ± 0.569
7.143 ± 0.729
1.227 ± 0.369
2.98 ± 0.348
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here