Pedobacter hartonius
Average proteome isoelectric point is 6.81
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 4634 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A1H3WDI3|A0A1H3WDI3_9SPHI 6-phosphogluconate dehydrogenase decarboxylating OS=Pedobacter hartonius OX=425514 GN=SAMN05443550_101206 PE=3 SV=1
MM1 pKa = 7.88 KK2 pKa = 9.33 ITTYY6 pKa = 7.47 EE7 pKa = 3.87 QRR9 pKa = 11.84 DD10 pKa = 4.55 LIPLTISVLGTISFFNACNTLKK32 pKa = 10.9 YY33 pKa = 10.38 LVEE36 pKa = 4.32 TEE38 pKa = 3.99 FCDD41 pKa = 4.35 LKK43 pKa = 11.46 GLISTDD49 pKa = 3.44 TLDD52 pKa = 4.28 DD53 pKa = 3.87 SSIALLDD60 pKa = 3.68 VVDD63 pKa = 4.61 NDD65 pKa = 3.61 FHH67 pKa = 7.9 KK68 pKa = 10.06 ITYY71 pKa = 10.56 DD72 pKa = 3.19 SMTGIYY78 pKa = 7.52 EE79 pKa = 4.41 TAHH82 pKa = 6.15 SLANIYY88 pKa = 10.68 GLDD91 pKa = 3.09 IGTIIRR97 pKa = 11.84 DD98 pKa = 3.43 EE99 pKa = 4.4 RR100 pKa = 11.84 LTEE103 pKa = 3.85 MGEE106 pKa = 4.14 DD107 pKa = 3.74 FVLEE111 pKa = 4.64 LIVDD115 pKa = 3.81 QFPEE119 pKa = 4.45 DD120 pKa = 3.62 NFVDD124 pKa = 3.9 EE125 pKa = 5.12 DD126 pKa = 4.07 FEE128 pKa = 4.45 PAEE131 pKa = 4.29 SLDD134 pKa = 3.78 LFAFSLVNVVNFLCSMLLAGKK155 pKa = 10.21 VDD157 pKa = 5.91 FDD159 pKa = 5.6 LSWTSSKK166 pKa = 10.67 FYY168 pKa = 11.03 LDD170 pKa = 4.76 FLSEE174 pKa = 4.42 DD175 pKa = 3.86 YY176 pKa = 10.81 SGKK179 pKa = 9.55 EE180 pKa = 3.54 HH181 pKa = 6.73 ATALQLLQEE190 pKa = 4.65 LGSVLSLSHH199 pKa = 6.69 GRR201 pKa = 11.84 ILDD204 pKa = 3.77 LLYY207 pKa = 11.29 NNGDD211 pKa = 3.65 ICWDD215 pKa = 3.7 DD216 pKa = 3.45 PP217 pKa = 4.23
Molecular weight: 24.48 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.731
IPC2_protein 3.821
IPC_protein 3.834
Toseland 3.617
ProMoST 3.999
Dawson 3.821
Bjellqvist 3.973
Wikipedia 3.757
Rodwell 3.656
Grimsley 3.516
Solomon 3.821
Lehninger 3.77
Nozaki 3.935
DTASelect 4.177
Thurlkill 3.668
EMBOSS 3.77
Sillero 3.948
Patrickios 1.888
IPC_peptide 3.808
IPC2_peptide 3.935
IPC2.peptide.svr19 3.851
Protein with the highest isoelectric point:
>tr|A0A1H3ZSW0|A0A1H3ZSW0_9SPHI Thiamine-phosphate pyrophosphorylase OS=Pedobacter hartonius OX=425514 GN=SAMN05443550_102480 PE=4 SV=1
MM1 pKa = 7.84 PSGKK5 pKa = 9.41 KK6 pKa = 9.7 RR7 pKa = 11.84 KK8 pKa = 6.09 SHH10 pKa = 6.26 KK11 pKa = 9.67 MATHH15 pKa = 5.94 KK16 pKa = 10.35 RR17 pKa = 11.84 KK18 pKa = 9.84 KK19 pKa = 9.28 RR20 pKa = 11.84 LRR22 pKa = 11.84 KK23 pKa = 9.26 NRR25 pKa = 11.84 HH26 pKa = 4.71 KK27 pKa = 10.87 KK28 pKa = 9.36 KK29 pKa = 10.77
Molecular weight: 3.61 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.41
IPC2_protein 10.95
IPC_protein 12.442
Toseland 12.647
ProMoST 13.1
Dawson 12.647
Bjellqvist 12.618
Wikipedia 13.1
Rodwell 12.676
Grimsley 12.676
Solomon 13.115
Lehninger 13.027
Nozaki 12.632
DTASelect 12.618
Thurlkill 12.632
EMBOSS 13.13
Sillero 12.632
Patrickios 12.398
IPC_peptide 13.115
IPC2_peptide 12.091
IPC2.peptide.svr19 8.949
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
4634
0
4634
1541803
29
3662
332.7
37.25
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
7.581 ± 0.035
0.777 ± 0.01
5.331 ± 0.027
5.742 ± 0.042
4.9 ± 0.026
6.867 ± 0.036
1.877 ± 0.018
7.319 ± 0.034
6.831 ± 0.041
9.728 ± 0.038
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.34 ± 0.017
5.433 ± 0.034
3.803 ± 0.021
3.648 ± 0.023
4.062 ± 0.022
6.557 ± 0.034
5.772 ± 0.04
6.267 ± 0.026
1.108 ± 0.015
4.056 ± 0.025
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here