Xanthomonadaceae bacterium NML03-0222

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Gammaproteobacteria; Xanthomonadales; Xanthomonadaceae; unclassified Xanthomonadaceae

Average proteome isoelectric point is 6.71

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2067 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A2M9E6V1|A0A2M9E6V1_9GAMM Endonuclease V OS=Xanthomonadaceae bacterium NML03-0222 OX=2032581 GN=nfi PE=3 SV=1
MM1 pKa = 7.47SEE3 pKa = 4.39SPASPVPFRR12 pKa = 11.84TWMCVVCGFIYY23 pKa = 10.67DD24 pKa = 3.92EE25 pKa = 4.44AQGLPEE31 pKa = 4.41EE32 pKa = 5.46GIAPGTRR39 pKa = 11.84WQDD42 pKa = 3.1IPDD45 pKa = 4.16DD46 pKa = 4.1WTCPDD51 pKa = 4.22CGVGKK56 pKa = 10.22DD57 pKa = 3.86DD58 pKa = 6.11FDD60 pKa = 3.77MQEE63 pKa = 3.62VV64 pKa = 3.44

Molecular weight: 7.13 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A2M9E0K7|A0A2M9E0K7_9GAMM Amidohydrolase OS=Xanthomonadaceae bacterium NML03-0222 OX=2032581 GN=CO611_07845 PE=4 SV=1
MM1 pKa = 7.28MVTIQQGLRR10 pKa = 11.84EE11 pKa = 4.58DD12 pKa = 3.78GFEE15 pKa = 3.86VSMVKK20 pKa = 10.25LCRR23 pKa = 11.84WFGVARR29 pKa = 11.84RR30 pKa = 11.84SVYY33 pKa = 10.21YY34 pKa = 10.18RR35 pKa = 11.84PTKK38 pKa = 10.24AAPKK42 pKa = 9.97VNPALAKK49 pKa = 9.8PIKK52 pKa = 10.17ALIEE56 pKa = 4.12AEE58 pKa = 4.03PSFGYY63 pKa = 8.7RR64 pKa = 11.84TVAGLLGMNKK74 pKa = 8.28NTVQRR79 pKa = 11.84IFQLKK84 pKa = 7.71GWQVRR89 pKa = 11.84KK90 pKa = 9.35RR91 pKa = 11.84AVGRR95 pKa = 11.84RR96 pKa = 11.84PRR98 pKa = 11.84IQALPSVATAPDD110 pKa = 3.97PRR112 pKa = 11.84WATDD116 pKa = 3.31LCRR119 pKa = 11.84VWGGRR124 pKa = 11.84DD125 pKa = 3.05GWLTLALVIDD135 pKa = 4.07CHH137 pKa = 6.05TRR139 pKa = 11.84QLLGWHH145 pKa = 7.13LSRR148 pKa = 11.84SGKK151 pKa = 10.58ASTAVAALEE160 pKa = 3.81QALIARR166 pKa = 11.84YY167 pKa = 7.27GTLGRR172 pKa = 11.84VPEE175 pKa = 4.23PFLLRR180 pKa = 11.84SDD182 pKa = 3.61NGLVFTSRR190 pKa = 11.84DD191 pKa = 3.27YY192 pKa = 10.56TRR194 pKa = 11.84LVRR197 pKa = 11.84SYY199 pKa = 11.26GLQQEE204 pKa = 5.57FITPHH209 pKa = 6.55CPQQNGMVEE218 pKa = 3.95RR219 pKa = 11.84LIRR222 pKa = 11.84TLKK225 pKa = 7.64EE226 pKa = 3.87QCVHH230 pKa = 5.07RR231 pKa = 11.84HH232 pKa = 4.88RR233 pKa = 11.84FEE235 pKa = 4.29SQQHH239 pKa = 4.7AMRR242 pKa = 11.84VLGDD246 pKa = 3.82WIQFYY251 pKa = 8.02NHH253 pKa = 6.68RR254 pKa = 11.84RR255 pKa = 11.84PHH257 pKa = 5.38QALGMKK263 pKa = 9.24TPAEE267 pKa = 4.41AYY269 pKa = 10.25ALAAA273 pKa = 4.47

Molecular weight: 31.06 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2067

0

2067

679785

29

2084

328.9

36.0

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

12.596 ± 0.08

0.869 ± 0.018

5.355 ± 0.035

5.813 ± 0.051

3.41 ± 0.034

8.049 ± 0.053

2.425 ± 0.029

4.69 ± 0.036

3.31 ± 0.041

11.033 ± 0.072

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.418 ± 0.027

2.874 ± 0.042

4.95 ± 0.037

4.581 ± 0.042

7.169 ± 0.051

5.393 ± 0.037

4.496 ± 0.042

6.66 ± 0.051

1.559 ± 0.03

2.35 ± 0.032
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski