Arthrobacter phage Melons

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; Coralvirus; unclassified Coralvirus

Average proteome isoelectric point is 7.0

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 73 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A3G2KI10|A0A3G2KI10_9CAUD Deoxycytidylate deaminase OS=Arthrobacter phage Melons OX=2419962 GN=54 PE=4 SV=1
MM1 pKa = 7.48GNVSDD6 pKa = 4.32WEE8 pKa = 4.43VNGDD12 pKa = 3.8GFATGPVIEE21 pKa = 5.05VEE23 pKa = 4.19NGDD26 pKa = 3.71NPVTVSAVWTDD37 pKa = 3.14EE38 pKa = 5.03DD39 pKa = 4.99GVCVSVDD46 pKa = 3.26TGDD49 pKa = 3.94EE50 pKa = 3.94PLKK53 pKa = 11.06ARR55 pKa = 11.84VAYY58 pKa = 8.99RR59 pKa = 11.84VCAAIMEE66 pKa = 4.72LAAVSAPEE74 pKa = 4.29LSSSS78 pKa = 3.39

Molecular weight: 8.07 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A3G2KHZ5|A0A3G2KHZ5_9CAUD Uncharacterized protein OS=Arthrobacter phage Melons OX=2419962 GN=60 PE=4 SV=1
MM1 pKa = 7.37SLGRR5 pKa = 11.84SGPFSWSGPSTFRR18 pKa = 11.84RR19 pKa = 11.84HH20 pKa = 4.34SAIIRR25 pKa = 11.84VSARR29 pKa = 11.84KK30 pKa = 9.13NN31 pKa = 3.13

Molecular weight: 3.45 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

73

0

73

12332

31

711

168.9

18.21

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

12.723 ± 0.403

0.86 ± 0.127

5.457 ± 0.199

6.56 ± 0.274

2.652 ± 0.173

9.861 ± 0.399

1.395 ± 0.158

3.56 ± 0.156

3.584 ± 0.255

9.074 ± 0.28

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.076 ± 0.108

2.376 ± 0.177

5.595 ± 0.336

2.984 ± 0.186

7.874 ± 0.397

5.433 ± 0.259

5.376 ± 0.32

8.539 ± 0.381

2.092 ± 0.149

1.93 ± 0.147
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski