Diporeia-associated CRESS-DNA virus LH481

Taxonomy: Viruses; unclassified viruses

Average proteome isoelectric point is 8.84

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1X8VHH5|A0A1X8VHH5_9VIRU Putative capsid protein OS=Diporeia-associated CRESS-DNA virus LH481 OX=1974422 GN=Cap PE=4 SV=1
MM1 pKa = 7.25TIKK4 pKa = 9.35MQNGQGTYY12 pKa = 9.55WILTIPRR19 pKa = 11.84EE20 pKa = 4.18SFVPYY25 pKa = 10.07LPPGIAYY32 pKa = 9.53IKK34 pKa = 10.14GQMEE38 pKa = 4.44IGASGYY44 pKa = 7.94NHH46 pKa = 6.13WQIIAICDD54 pKa = 3.27RR55 pKa = 11.84SRR57 pKa = 11.84RR58 pKa = 11.84LSWIRR63 pKa = 11.84SIFGNFHH70 pKa = 6.87AEE72 pKa = 4.01LTRR75 pKa = 11.84SVAAEE80 pKa = 3.73QYY82 pKa = 8.54VWKK85 pKa = 10.65DD86 pKa = 3.25EE87 pKa = 4.22TAVVGTRR94 pKa = 11.84FEE96 pKa = 5.02LGSKK100 pKa = 9.7PIRR103 pKa = 11.84RR104 pKa = 11.84NAKK107 pKa = 9.81KK108 pKa = 10.24DD109 pKa = 2.77WDD111 pKa = 5.01AIWSSACSGDD121 pKa = 3.31ILAIPSDD128 pKa = 3.65VRR130 pKa = 11.84IRR132 pKa = 11.84CYY134 pKa = 10.0STLKK138 pKa = 10.49RR139 pKa = 11.84IRR141 pKa = 11.84ADD143 pKa = 3.29YY144 pKa = 10.56LEE146 pKa = 4.63PVSMVRR152 pKa = 11.84SCTVFWGPTGTGKK165 pKa = 10.79SMRR168 pKa = 11.84SWNEE172 pKa = 3.28AGLQAYY178 pKa = 8.1PKK180 pKa = 10.65DD181 pKa = 3.71PNTKK185 pKa = 9.1FWCGYY190 pKa = 9.04SGQEE194 pKa = 3.62HH195 pKa = 5.24VVIDD199 pKa = 3.85EE200 pKa = 4.08FRR202 pKa = 11.84GKK204 pKa = 9.97IDD206 pKa = 3.24ISHH209 pKa = 7.64LLRR212 pKa = 11.84WLDD215 pKa = 3.65RR216 pKa = 11.84YY217 pKa = 9.3PVRR220 pKa = 11.84VEE222 pKa = 3.91LKK224 pKa = 9.7GASFPLMATKK234 pKa = 10.53YY235 pKa = 8.8WITSNLHH242 pKa = 4.74PRR244 pKa = 11.84EE245 pKa = 4.3WYY247 pKa = 9.92PEE249 pKa = 3.68IDD251 pKa = 3.5AYY253 pKa = 10.05TYY255 pKa = 10.65DD256 pKa = 3.43ALEE259 pKa = 4.06RR260 pKa = 11.84RR261 pKa = 11.84LNIINITT268 pKa = 3.55

Molecular weight: 31.0 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1X8VHH5|A0A1X8VHH5_9VIRU Putative capsid protein OS=Diporeia-associated CRESS-DNA virus LH481 OX=1974422 GN=Cap PE=4 SV=1
MM1 pKa = 7.26GLKK4 pKa = 10.25RR5 pKa = 11.84KK6 pKa = 9.0IANAAGATLGFIAGDD21 pKa = 3.3IPGAMTGADD30 pKa = 4.14LADD33 pKa = 4.35SLYY36 pKa = 10.18KK37 pKa = 9.72WKK39 pKa = 11.14YY40 pKa = 8.35PDD42 pKa = 3.25QKK44 pKa = 11.47NFAEE48 pKa = 4.9KK49 pKa = 10.39INMGKK54 pKa = 9.4YY55 pKa = 9.37VKK57 pKa = 10.38SGSKK61 pKa = 10.1RR62 pKa = 11.84KK63 pKa = 9.61ASKK66 pKa = 10.12QSGGKK71 pKa = 8.5RR72 pKa = 11.84RR73 pKa = 11.84KK74 pKa = 8.12VSKK77 pKa = 10.71YY78 pKa = 9.57SGNKK82 pKa = 9.24KK83 pKa = 8.89VEE85 pKa = 3.85KK86 pKa = 9.79MRR88 pKa = 11.84NNNMNQTNLTNTHH101 pKa = 6.0IKK103 pKa = 10.63KK104 pKa = 10.22KK105 pKa = 9.68DD106 pKa = 3.39TLKK109 pKa = 9.99MRR111 pKa = 11.84KK112 pKa = 8.73SPYY115 pKa = 9.13KK116 pKa = 10.39VRR118 pKa = 11.84VSSAFRR124 pKa = 11.84KK125 pKa = 9.72KK126 pKa = 10.36VIKK129 pKa = 10.28AVSDD133 pKa = 3.77KK134 pKa = 10.58EE135 pKa = 4.35PQGSATLTFIGGFFPPNGNVGWPVRR160 pKa = 11.84DD161 pKa = 3.58SVSKK165 pKa = 10.19QWVFGLPMGNQATSATAYY183 pKa = 10.99SNGANIRR190 pKa = 11.84SGDD193 pKa = 3.73VFRR196 pKa = 11.84TNMLLHH202 pKa = 5.93VASRR206 pKa = 11.84MWNGAAKK213 pKa = 8.83ATNFNEE219 pKa = 4.01FGYY222 pKa = 10.97DD223 pKa = 3.77RR224 pKa = 11.84LDD226 pKa = 3.52NFCNPEE232 pKa = 4.0TSVGALKK239 pKa = 10.76LDD241 pKa = 3.75ILKK244 pKa = 9.94SWAVIEE250 pKa = 4.33MKK252 pKa = 10.72NVTRR256 pKa = 11.84TKK258 pKa = 10.47LRR260 pKa = 11.84LYY262 pKa = 10.18MYY264 pKa = 9.71VCKK267 pKa = 10.1PKK269 pKa = 9.63YY270 pKa = 8.7QRR272 pKa = 11.84RR273 pKa = 11.84TADD276 pKa = 3.03FNARR280 pKa = 11.84VDD282 pKa = 4.0GVNSGIPTQFTEE294 pKa = 4.33FGTNISGVQASTYY307 pKa = 10.76GIFPEE312 pKa = 4.54MTPAWKK318 pKa = 9.92RR319 pKa = 11.84QWEE322 pKa = 4.24FEE324 pKa = 3.94KK325 pKa = 11.38YY326 pKa = 10.3EE327 pKa = 4.02IVLEE331 pKa = 4.2PGQEE335 pKa = 3.84HH336 pKa = 6.45RR337 pKa = 11.84HH338 pKa = 5.66TINGYY343 pKa = 9.52CGQFDD348 pKa = 3.82FLKK351 pKa = 10.24AYY353 pKa = 9.59KK354 pKa = 10.41DD355 pKa = 3.68SVHH358 pKa = 6.73QNVQPIDD365 pKa = 3.34RR366 pKa = 11.84HH367 pKa = 5.04VFFVMQTDD375 pKa = 3.89LGIVRR380 pKa = 11.84EE381 pKa = 4.33GTPAVSYY388 pKa = 9.66GVRR391 pKa = 11.84TAQDD395 pKa = 3.1SDD397 pKa = 3.97DD398 pKa = 4.03ASLGLGILFEE408 pKa = 4.75TKK410 pKa = 9.5VHH412 pKa = 5.86WTLQMPEE419 pKa = 3.86PAGARR424 pKa = 11.84MPATGLLGVGAYY436 pKa = 8.19NKK438 pKa = 9.75PAGFRR443 pKa = 11.84KK444 pKa = 9.57RR445 pKa = 11.84CYY447 pKa = 10.31LYY449 pKa = 10.49DD450 pKa = 5.27IIDD453 pKa = 4.34DD454 pKa = 3.92TDD456 pKa = 4.02LPNQTEE462 pKa = 4.47VVNIDD467 pKa = 3.74DD468 pKa = 4.27NNPTVVVAA476 pKa = 5.12

Molecular weight: 53.1 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2

0

2

744

268

476

372.0

42.05

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.392 ± 0.404

1.21 ± 0.392

5.242 ± 0.234

4.167 ± 0.631

4.032 ± 0.625

7.93 ± 0.725

1.613 ± 0.151

6.183 ± 1.878

7.392 ± 1.518

5.914 ± 0.256

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.823 ± 0.349

5.511 ± 1.285

4.839 ± 0.23

3.36 ± 0.447

6.452 ± 1.049

6.317 ± 0.684

6.183 ± 0.35

6.452 ± 0.956

2.688 ± 1.069

4.301 ± 0.551
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski