Dyadobacter psychrotolerans

Taxonomy: cellular organisms; Bacteria; FCB group; Bacteroidetes/Chlorobi group; Bacteroidetes; Cytophagia; Cytophagales; Cytophagaceae; Dyadobacter

Average proteome isoelectric point is 6.78

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 6512 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A4R5DXD7|A0A4R5DXD7_9BACT D-2-hydroxyacid dehydrogenase family protein OS=Dyadobacter psychrotolerans OX=2541721 GN=E0F88_04195 PE=3 SV=1
MM1 pKa = 7.04TQDD4 pKa = 2.76NCYY7 pKa = 10.21LLGYY11 pKa = 9.62IVRR14 pKa = 11.84THH16 pKa = 5.55GTSGNVVIFLDD27 pKa = 3.33VDD29 pKa = 3.64YY30 pKa = 10.98PDD32 pKa = 5.58DD33 pKa = 4.87YY34 pKa = 11.99EE35 pKa = 6.1DD36 pKa = 4.49LDD38 pKa = 3.93SVYY41 pKa = 10.99VEE43 pKa = 4.58LKK45 pKa = 10.99GEE47 pKa = 4.06LVPYY51 pKa = 9.71FISNFNLQKK60 pKa = 10.27QSNAIVTFEE69 pKa = 4.71DD70 pKa = 3.11VDD72 pKa = 4.37TIEE75 pKa = 4.48KK76 pKa = 10.18AQALVGSSLFLSLDD90 pKa = 3.46EE91 pKa = 4.83LDD93 pKa = 3.74EE94 pKa = 4.84LEE96 pKa = 4.88GEE98 pKa = 3.95EE99 pKa = 4.93FYY101 pKa = 11.45YY102 pKa = 10.92HH103 pKa = 7.14EE104 pKa = 4.51IKK106 pKa = 10.74GYY108 pKa = 7.76TVVDD112 pKa = 3.68EE113 pKa = 4.79TKK115 pKa = 10.89GEE117 pKa = 4.04LGIVRR122 pKa = 11.84EE123 pKa = 4.42VYY125 pKa = 10.6SLNGQDD131 pKa = 5.41LIAMDD136 pKa = 4.04YY137 pKa = 10.37QGHH140 pKa = 5.75EE141 pKa = 4.18VLIPTAEE148 pKa = 4.7DD149 pKa = 3.03IVLKK153 pKa = 10.58ADD155 pKa = 3.83KK156 pKa = 9.98EE157 pKa = 4.33NKK159 pKa = 9.65KK160 pKa = 10.83LIVNLPEE167 pKa = 4.52GLLEE171 pKa = 4.93VYY173 pKa = 10.71LEE175 pKa = 4.99DD176 pKa = 5.05PADD179 pKa = 3.89SKK181 pKa = 11.28NAPDD185 pKa = 5.13DD186 pKa = 4.12ADD188 pKa = 3.32

Molecular weight: 21.16 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A4R5DCX3|A0A4R5DCX3_9BACT Pyruvate kinase OS=Dyadobacter psychrotolerans OX=2541721 GN=pyk PE=3 SV=1
MM1 pKa = 7.58EE2 pKa = 4.88NSFTKK7 pKa = 10.57TSATSLSLGSLLSLVTMLLHH27 pKa = 6.5PSGGSIEE34 pKa = 4.67HH35 pKa = 7.54IIRR38 pKa = 11.84MRR40 pKa = 11.84HH41 pKa = 4.79ILIFSHH47 pKa = 6.41VLAIACLPLLGFGAWGLSILLQTRR71 pKa = 11.84SRR73 pKa = 11.84ISTLIFFVFCFGLIAAMIAAAVNGLILPQFLSASSKK109 pKa = 10.62AASQQLMLRR118 pKa = 11.84TVVNYY123 pKa = 8.89GHH125 pKa = 6.98HH126 pKa = 6.14MNISLANIFIFASSLSIMAWCILIIRR152 pKa = 11.84SGLLPRR158 pKa = 11.84WTGHH162 pKa = 6.49FGLLLFGFGIGCFLLKK178 pKa = 11.02VNFTALYY185 pKa = 8.94GFRR188 pKa = 11.84IFVAGLAIWMIIAGLQMILTVKK210 pKa = 10.81SNIKK214 pKa = 10.02KK215 pKa = 10.35

Molecular weight: 23.46 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

6512

0

6512

2294528

25

2398

352.4

39.5

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.264 ± 0.03

0.727 ± 0.011

5.315 ± 0.02

5.891 ± 0.028

4.961 ± 0.022

6.951 ± 0.031

1.77 ± 0.014

7.012 ± 0.024

6.783 ± 0.024

9.595 ± 0.032

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.283 ± 0.013

5.27 ± 0.03

3.885 ± 0.017

3.81 ± 0.02

4.256 ± 0.019

6.851 ± 0.025

5.711 ± 0.025

6.474 ± 0.024

1.266 ± 0.013

3.923 ± 0.019
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski