Shewanella sp. SNU WT4

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Gammaproteobacteria; Alteromonadales; Shewanellaceae; Shewanella; unclassified Shewanella

Average proteome isoelectric point is 6.26

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3378 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A4Y6IAL5|A0A4Y6IAL5_9GAMM Glutamate-1-semialdehyde 2 1-aminomutase OS=Shewanella sp. SNU WT4 OX=2590015 GN=hemL PE=3 SV=1
MM1 pKa = 7.83SIQQEE6 pKa = 4.06LQEE9 pKa = 4.0QFEE12 pKa = 4.66EE13 pKa = 3.88NRR15 pKa = 11.84EE16 pKa = 3.93IVEE19 pKa = 4.2ALLADD24 pKa = 4.28GSEE27 pKa = 3.84VDD29 pKa = 3.38ALYY32 pKa = 9.97TIEE35 pKa = 5.0HH36 pKa = 6.74HH37 pKa = 6.6FCSTNFDD44 pKa = 4.07KK45 pKa = 11.41LEE47 pKa = 4.15KK48 pKa = 10.37AAVDD52 pKa = 3.47AFKK55 pKa = 11.08LGFEE59 pKa = 4.36VNDD62 pKa = 3.74AEE64 pKa = 4.72EE65 pKa = 4.3MEE67 pKa = 4.5LEE69 pKa = 3.97EE70 pKa = 5.69GGTIFCFDD78 pKa = 5.32AIAKK82 pKa = 9.69HH83 pKa = 5.86LLSVDD88 pKa = 4.66LLDD91 pKa = 4.07QACEE95 pKa = 3.86QLMQLAAKK103 pKa = 10.36HH104 pKa = 4.88NVEE107 pKa = 3.8YY108 pKa = 10.77DD109 pKa = 2.83GWGTYY114 pKa = 10.42YY115 pKa = 10.13IGDD118 pKa = 3.92DD119 pKa = 4.16AEE121 pKa = 4.76IDD123 pKa = 3.76EE124 pKa = 5.84DD125 pKa = 5.45DD126 pKa = 5.76LDD128 pKa = 5.0DD129 pKa = 5.07EE130 pKa = 4.63PAEE133 pKa = 4.37APWNN137 pKa = 3.74

Molecular weight: 15.53 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A4Y6I7L5|A0A4Y6I7L5_9GAMM Guanylate kinase OS=Shewanella sp. SNU WT4 OX=2590015 GN=gmk PE=3 SV=1
MM1 pKa = 7.69SKK3 pKa = 9.01RR4 pKa = 11.84TFQPSNLKK12 pKa = 10.14RR13 pKa = 11.84KK14 pKa = 9.13RR15 pKa = 11.84SHH17 pKa = 6.17GFRR20 pKa = 11.84ARR22 pKa = 11.84MATVGGRR29 pKa = 11.84KK30 pKa = 9.01VLARR34 pKa = 11.84RR35 pKa = 11.84RR36 pKa = 11.84AKK38 pKa = 10.39GRR40 pKa = 11.84ARR42 pKa = 11.84LSAA45 pKa = 3.91

Molecular weight: 5.15 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3378

0

3378

1121467

21

2135

332.0

36.65

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

9.825 ± 0.046

1.154 ± 0.015

5.366 ± 0.034

5.24 ± 0.037

3.827 ± 0.027

6.8 ± 0.039

2.401 ± 0.02

6.2 ± 0.033

4.688 ± 0.034

11.125 ± 0.064

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.722 ± 0.023

4.121 ± 0.034

4.046 ± 0.025

5.253 ± 0.037

4.526 ± 0.037

6.538 ± 0.035

5.278 ± 0.034

6.709 ± 0.035

1.281 ± 0.018

2.899 ± 0.025
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski