Amino acid dipepetide frequency for Shewanella sp. SNU WT4

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
9.82AlaAla: 9.82 ± 0.131
1.241AlaCys: 1.241 ± 0.033
5.047AlaAsp: 5.047 ± 0.073
5.37AlaGlu: 5.37 ± 0.075
3.3AlaPhe: 3.3 ± 0.062
6.567AlaGly: 6.567 ± 0.096
1.924AlaHis: 1.924 ± 0.043
6.79AlaIle: 6.79 ± 0.09
5.635AlaLys: 5.635 ± 0.086
11.262AlaLeu: 11.262 ± 0.124
3.208AlaMet: 3.208 ± 0.062
4.563AlaAsn: 4.563 ± 0.062
3.656AlaPro: 3.656 ± 0.07
4.779AlaGln: 4.779 ± 0.066
4.101AlaArg: 4.101 ± 0.076
6.349AlaSer: 6.349 ± 0.09
5.01AlaThr: 5.01 ± 0.077
6.071AlaVal: 6.071 ± 0.068
1.122AlaTrp: 1.122 ± 0.033
2.439AlaTyr: 2.439 ± 0.05
0.0AlaXaa: 0.0 ± 0.0
Cys
0.926CysAla: 0.926 ± 0.031
0.205CysCys: 0.205 ± 0.016
0.698CysAsp: 0.698 ± 0.025
0.608CysGlu: 0.608 ± 0.024
0.461CysPhe: 0.461 ± 0.02
0.955CysGly: 0.955 ± 0.033
0.526CysHis: 0.526 ± 0.034
0.62CysIle: 0.62 ± 0.023
0.365CysLys: 0.365 ± 0.018
1.197CysLeu: 1.197 ± 0.037
0.241CysMet: 0.241 ± 0.016
0.357CysAsn: 0.357 ± 0.017
0.539CysPro: 0.539 ± 0.027
0.793CysGln: 0.793 ± 0.031
0.589CysArg: 0.589 ± 0.022
0.768CysSer: 0.768 ± 0.029
0.457CysThr: 0.457 ± 0.019
0.731CysVal: 0.731 ± 0.023
0.149CysTrp: 0.149 ± 0.011
0.359CysTyr: 0.359 ± 0.02
0.0CysXaa: 0.0 ± 0.0
Asp
5.003AspAla: 5.003 ± 0.074
0.637AspCys: 0.637 ± 0.025
3.064AspAsp: 3.064 ± 0.061
3.271AspGlu: 3.271 ± 0.061
2.351AspPhe: 2.351 ± 0.051
3.752AspGly: 3.752 ± 0.077
1.009AspHis: 1.009 ± 0.034
4.083AspIle: 4.083 ± 0.063
3.279AspLys: 3.279 ± 0.057
4.887AspLeu: 4.887 ± 0.078
1.577AspMet: 1.577 ± 0.041
2.694AspAsn: 2.694 ± 0.05
1.947AspPro: 1.947 ± 0.045
1.555AspGln: 1.555 ± 0.038
1.975AspArg: 1.975 ± 0.048
3.43AspSer: 3.43 ± 0.06
2.773AspThr: 2.773 ± 0.053
3.593AspVal: 3.593 ± 0.057
0.806AspTrp: 0.806 ± 0.027
1.972AspTyr: 1.972 ± 0.045
0.0AspXaa: 0.0 ± 0.0
Glu
5.031GluAla: 5.031 ± 0.083
0.531GluCys: 0.531 ± 0.023
2.139GluAsp: 2.139 ± 0.047
2.277GluGlu: 2.277 ± 0.061
2.228GluPhe: 2.228 ± 0.045
2.82GluGly: 2.82 ± 0.058
1.536GluHis: 1.536 ± 0.035
3.164GluIle: 3.164 ± 0.06
2.264GluLys: 2.264 ± 0.057
7.011GluLeu: 7.011 ± 0.09
1.377GluMet: 1.377 ± 0.037
1.782GluAsn: 1.782 ± 0.046
1.934GluPro: 1.934 ± 0.045
4.059GluGln: 4.059 ± 0.063
3.09GluArg: 3.09 ± 0.059
2.749GluSer: 2.749 ± 0.052
2.523GluThr: 2.523 ± 0.048
3.767GluVal: 3.767 ± 0.063
0.614GluTrp: 0.614 ± 0.026
1.504GluTyr: 1.504 ± 0.038
0.0GluXaa: 0.0 ± 0.0
Phe
3.835PheAla: 3.835 ± 0.058
0.499PheCys: 0.499 ± 0.024
2.634PheAsp: 2.634 ± 0.047
2.161PheGlu: 2.161 ± 0.046
1.463PhePhe: 1.463 ± 0.045
3.072PheGly: 3.072 ± 0.064
0.774PheHis: 0.774 ± 0.026
2.596PheIle: 2.596 ± 0.051
1.721PheLys: 1.721 ± 0.036
2.91PheLeu: 2.91 ± 0.059
1.055PheMet: 1.055 ± 0.031
2.034PheAsn: 2.034 ± 0.045
1.317PhePro: 1.317 ± 0.035
1.09PheGln: 1.09 ± 0.027
1.371PheArg: 1.371 ± 0.036
3.197PheSer: 3.197 ± 0.049
2.231PheThr: 2.231 ± 0.043
2.527PheVal: 2.527 ± 0.06
0.478PheTrp: 0.478 ± 0.022
1.303PheTyr: 1.303 ± 0.036
0.0PheXaa: 0.0 ± 0.0
Gly
5.915GlyAla: 5.915 ± 0.098
0.935GlyCys: 0.935 ± 0.03
3.542GlyAsp: 3.542 ± 0.063
3.652GlyGlu: 3.652 ± 0.064
3.232GlyPhe: 3.232 ± 0.058
4.638GlyGly: 4.638 ± 0.09
1.763GlyHis: 1.763 ± 0.037
4.683GlyIle: 4.683 ± 0.079
3.733GlyLys: 3.733 ± 0.06
7.484GlyLeu: 7.484 ± 0.087
2.056GlyMet: 2.056 ± 0.048
2.61GlyAsn: 2.61 ± 0.057
1.718GlyPro: 1.718 ± 0.046
3.3GlyGln: 3.3 ± 0.063
3.205GlyArg: 3.205 ± 0.063
3.964GlySer: 3.964 ± 0.064
3.187GlyThr: 3.187 ± 0.056
5.067GlyVal: 5.067 ± 0.071
0.897GlyTrp: 0.897 ± 0.028
2.416GlyTyr: 2.416 ± 0.054
0.0GlyXaa: 0.0 ± 0.0
His
1.821HisAla: 1.821 ± 0.042
0.368HisCys: 0.368 ± 0.017
1.186HisAsp: 1.186 ± 0.033
1.102HisGlu: 1.102 ± 0.03
1.119HisPhe: 1.119 ± 0.034
1.774HisGly: 1.774 ± 0.038
0.744HisHis: 0.744 ± 0.028
1.435HisIle: 1.435 ± 0.036
1.058HisLys: 1.058 ± 0.03
2.63HisLeu: 2.63 ± 0.055
0.597HisMet: 0.597 ± 0.025
0.982HisAsn: 0.982 ± 0.035
1.229HisPro: 1.229 ± 0.029
1.604HisGln: 1.604 ± 0.044
0.985HisArg: 0.985 ± 0.029
1.604HisSer: 1.604 ± 0.039
1.113HisThr: 1.113 ± 0.036
1.213HisVal: 1.213 ± 0.034
0.49HisTrp: 0.49 ± 0.02
0.959HisTyr: 0.959 ± 0.028
0.0HisXaa: 0.0 ± 0.0
Ile
6.896IleAla: 6.896 ± 0.09
0.694IleCys: 0.694 ± 0.025
4.061IleAsp: 4.061 ± 0.062
4.053IleGlu: 4.053 ± 0.061
2.022IlePhe: 2.022 ± 0.048
4.507IleGly: 4.507 ± 0.073
1.308IleHis: 1.308 ± 0.033
3.802IleIle: 3.802 ± 0.064
3.208IleLys: 3.208 ± 0.054
5.094IleLeu: 5.094 ± 0.076
1.45IleMet: 1.45 ± 0.036
3.412IleAsn: 3.412 ± 0.061
2.472IlePro: 2.472 ± 0.045
2.086IleGln: 2.086 ± 0.045
2.672IleArg: 2.672 ± 0.055
4.563IleSer: 4.563 ± 0.069
3.593IleThr: 3.593 ± 0.064
3.786IleVal: 3.786 ± 0.073
0.592IleTrp: 0.592 ± 0.018
1.731IleTyr: 1.731 ± 0.034
0.0IleXaa: 0.0 ± 0.0
Lys
4.742LysAla: 4.742 ± 0.072
0.332LysCys: 0.332 ± 0.019
2.324LysAsp: 2.324 ± 0.052
2.308LysGlu: 2.308 ± 0.052
1.407LysPhe: 1.407 ± 0.04
3.02LysGly: 3.02 ± 0.056
1.203LysHis: 1.203 ± 0.034
2.643LysIle: 2.643 ± 0.05
1.98LysLys: 1.98 ± 0.05
5.553LysLeu: 5.553 ± 0.072
1.275LysMet: 1.275 ± 0.034
1.611LysAsn: 1.611 ± 0.04
2.266LysPro: 2.266 ± 0.047
3.111LysGln: 3.111 ± 0.054
2.528LysArg: 2.528 ± 0.053
2.736LysSer: 2.736 ± 0.051
2.591LysThr: 2.591 ± 0.047
3.488LysVal: 3.488 ± 0.057
0.553LysTrp: 0.553 ± 0.021
1.213LysTyr: 1.213 ± 0.031
0.0LysXaa: 0.0 ± 0.0
Leu
12.62LeuAla: 12.62 ± 0.132
1.346LeuCys: 1.346 ± 0.035
6.022LeuAsp: 6.022 ± 0.079
5.472LeuGlu: 5.472 ± 0.081
3.948LeuPhe: 3.948 ± 0.074
7.554LeuGly: 7.554 ± 0.092
2.148LeuHis: 2.148 ± 0.053
6.301LeuIle: 6.301 ± 0.081
4.948LeuLys: 4.948 ± 0.071
12.328LeuLeu: 12.328 ± 0.173
3.021LeuMet: 3.021 ± 0.057
4.641LeuAsn: 4.641 ± 0.078
5.095LeuPro: 5.095 ± 0.077
4.56LeuGln: 4.56 ± 0.08
4.401LeuArg: 4.401 ± 0.075
8.135LeuSer: 8.135 ± 0.1
6.966LeuThr: 6.966 ± 0.088
7.861LeuVal: 7.861 ± 0.105
1.262LeuTrp: 1.262 ± 0.044
2.624LeuTyr: 2.624 ± 0.058
0.0LeuXaa: 0.0 ± 0.0
Met
3.222MetAla: 3.222 ± 0.053
0.235MetCys: 0.235 ± 0.013
1.317MetAsp: 1.317 ± 0.037
1.133MetGlu: 1.133 ± 0.037
0.883MetPhe: 0.883 ± 0.03
1.765MetGly: 1.765 ± 0.044
0.525MetHis: 0.525 ± 0.021
1.426MetIle: 1.426 ± 0.039
1.315MetLys: 1.315 ± 0.035
3.185MetLeu: 3.185 ± 0.056
0.82MetMet: 0.82 ± 0.028
1.004MetAsn: 1.004 ± 0.028
1.38MetPro: 1.38 ± 0.041
1.339MetGln: 1.339 ± 0.034
1.241MetArg: 1.241 ± 0.034
2.057MetSer: 2.057 ± 0.045
1.863MetThr: 1.863 ± 0.041
1.773MetVal: 1.773 ± 0.04
0.244MetTrp: 0.244 ± 0.015
0.489MetTyr: 0.489 ± 0.02
0.0MetXaa: 0.0 ± 0.0
Asn
3.939AsnAla: 3.939 ± 0.063
0.469AsnCys: 0.469 ± 0.021
2.341AsnAsp: 2.341 ± 0.055
2.038AsnGlu: 2.038 ± 0.044
1.445AsnPhe: 1.445 ± 0.035
2.898AsnGly: 2.898 ± 0.058
1.08AsnHis: 1.08 ± 0.034
2.655AsnIle: 2.655 ± 0.048
1.949AsnLys: 1.949 ± 0.039
4.069AsnLeu: 4.069 ± 0.066
0.999AsnMet: 0.999 ± 0.029
1.931AsnAsn: 1.931 ± 0.046
2.135AsnPro: 2.135 ± 0.04
2.379AsnGln: 2.379 ± 0.047
1.977AsnArg: 1.977 ± 0.038
2.58AsnSer: 2.58 ± 0.054
2.188AsnThr: 2.188 ± 0.05
2.377AsnVal: 2.377 ± 0.052
0.519AsnTrp: 0.519 ± 0.023
1.242AsnTyr: 1.242 ± 0.033
0.0AsnXaa: 0.0 ± 0.0
Pro
3.681ProAla: 3.681 ± 0.068
0.354ProCys: 0.354 ± 0.02
2.143ProAsp: 2.143 ± 0.042
2.825ProGlu: 2.825 ± 0.051
1.588ProPhe: 1.588 ± 0.039
2.355ProGly: 2.355 ± 0.049
0.924ProHis: 0.924 ± 0.031
2.451ProIle: 2.451 ± 0.052
2.173ProLys: 2.173 ± 0.041
4.771ProLeu: 4.771 ± 0.073
1.231ProMet: 1.231 ± 0.035
1.688ProAsn: 1.688 ± 0.037
1.216ProPro: 1.216 ± 0.034
2.053ProGln: 2.053 ± 0.045
1.437ProArg: 1.437 ± 0.039
2.784ProSer: 2.784 ± 0.052
2.055ProThr: 2.055 ± 0.037
2.979ProVal: 2.979 ± 0.054
0.62ProTrp: 0.62 ± 0.028
1.127ProTyr: 1.127 ± 0.032
0.0ProXaa: 0.0 ± 0.0
Gln
6.002GlnAla: 6.002 ± 0.086
0.526GlnCys: 0.526 ± 0.02
2.383GlnAsp: 2.383 ± 0.052
2.224GlnGlu: 2.224 ± 0.056
1.877GlnPhe: 1.877 ± 0.042
3.684GlnGly: 3.684 ± 0.062
1.563GlnHis: 1.563 ± 0.043
2.548GlnIle: 2.548 ± 0.052
1.733GlnLys: 1.733 ± 0.041
6.879GlnLeu: 6.879 ± 0.094
1.293GlnMet: 1.293 ± 0.034
1.245GlnAsn: 1.245 ± 0.034
1.791GlnPro: 1.791 ± 0.043
4.598GlnGln: 4.598 ± 0.098
2.492GlnArg: 2.492 ± 0.051
2.853GlnSer: 2.853 ± 0.048
2.497GlnThr: 2.497 ± 0.048
3.92GlnVal: 3.92 ± 0.065
0.886GlnTrp: 0.886 ± 0.029
1.539GlnTyr: 1.539 ± 0.036
0.0GlnXaa: 0.0 ± 0.0
Arg
3.696ArgAla: 3.696 ± 0.06
0.482ArgCys: 0.482 ± 0.02
2.405ArgAsp: 2.405 ± 0.049
2.477ArgGlu: 2.477 ± 0.057
2.103ArgPhe: 2.103 ± 0.043
2.64ArgGly: 2.64 ± 0.051
1.314ArgHis: 1.314 ± 0.036
2.86ArgIle: 2.86 ± 0.052
2.021ArgLys: 2.021 ± 0.049
5.375ArgLeu: 5.375 ± 0.069
1.164ArgMet: 1.164 ± 0.036
1.707ArgAsn: 1.707 ± 0.044
1.784ArgPro: 1.784 ± 0.047
2.877ArgGln: 2.877 ± 0.047
2.424ArgArg: 2.424 ± 0.058
2.564ArgSer: 2.564 ± 0.056
2.055ArgThr: 2.055 ± 0.046
2.923ArgVal: 2.923 ± 0.058
0.647ArgTrp: 0.647 ± 0.024
1.744ArgTyr: 1.744 ± 0.04
0.0ArgXaa: 0.0 ± 0.0
Ser
6.202SerAla: 6.202 ± 0.086
0.786SerCys: 0.786 ± 0.033
3.635SerAsp: 3.635 ± 0.061
3.369SerGlu: 3.369 ± 0.05
2.573SerPhe: 2.573 ± 0.049
4.825SerGly: 4.825 ± 0.074
1.825SerHis: 1.825 ± 0.043
3.766SerIle: 3.766 ± 0.056
2.754SerLys: 2.754 ± 0.054
7.889SerLeu: 7.889 ± 0.107
1.765SerMet: 1.765 ± 0.046
2.41SerAsn: 2.41 ± 0.045
2.608SerPro: 2.608 ± 0.053
3.593SerGln: 3.593 ± 0.061
3.155SerArg: 3.155 ± 0.055
4.223SerSer: 4.223 ± 0.08
2.897SerThr: 2.897 ± 0.051
4.234SerVal: 4.234 ± 0.063
0.871SerTrp: 0.871 ± 0.027
2.001SerTyr: 2.001 ± 0.053
0.0SerXaa: 0.0 ± 0.0
Thr
4.968ThrAla: 4.968 ± 0.091
0.516ThrCys: 0.516 ± 0.022
2.899ThrAsp: 2.899 ± 0.052
2.899ThrGlu: 2.899 ± 0.059
1.837ThrPhe: 1.837 ± 0.039
3.963ThrGly: 3.963 ± 0.063
1.302ThrHis: 1.302 ± 0.036
3.231ThrIle: 3.231 ± 0.069
2.19ThrLys: 2.19 ± 0.035
6.285ThrLeu: 6.285 ± 0.076
1.221ThrMet: 1.221 ± 0.034
2.09ThrAsn: 2.09 ± 0.052
2.803ThrPro: 2.803 ± 0.054
2.676ThrGln: 2.676 ± 0.055
2.264ThrArg: 2.264 ± 0.037
3.423ThrSer: 3.423 ± 0.058
2.876ThrThr: 2.876 ± 0.059
3.441ThrVal: 3.441 ± 0.059
0.612ThrTrp: 0.612 ± 0.022
1.289ThrTyr: 1.289 ± 0.039
0.0ThrXaa: 0.0 ± 0.0
Val
6.767ValAla: 6.767 ± 0.093
0.785ValCys: 0.785 ± 0.026
3.749ValAsp: 3.749 ± 0.064
3.532ValGlu: 3.532 ± 0.072
2.53ValPhe: 2.53 ± 0.052
4.336ValGly: 4.336 ± 0.061
1.28ValHis: 1.28 ± 0.038
4.852ValIle: 4.852 ± 0.072
3.172ValLys: 3.172 ± 0.059
6.928ValLeu: 6.928 ± 0.091
1.988ValMet: 1.988 ± 0.043
3.136ValAsn: 3.136 ± 0.063
2.56ValPro: 2.56 ± 0.042
2.22ValGln: 2.22 ± 0.037
2.807ValArg: 2.807 ± 0.051
4.804ValSer: 4.804 ± 0.072
4.248ValThr: 4.248 ± 0.072
4.884ValVal: 4.884 ± 0.084
0.762ValTrp: 0.762 ± 0.029
1.747ValTyr: 1.747 ± 0.043
0.0ValXaa: 0.0 ± 0.0
Trp
0.871TrpAla: 0.871 ± 0.028
0.168TrpCys: 0.168 ± 0.013
0.588TrpAsp: 0.588 ± 0.026
0.399TrpGlu: 0.399 ± 0.018
0.585TrpPhe: 0.585 ± 0.023
0.737TrpGly: 0.737 ± 0.026
0.439TrpHis: 0.439 ± 0.02
0.628TrpIle: 0.628 ± 0.028
0.35TrpLys: 0.35 ± 0.019
1.946TrpLeu: 1.946 ± 0.048
0.3TrpMet: 0.3 ± 0.016
0.363TrpAsn: 0.363 ± 0.018
0.573TrpPro: 0.573 ± 0.024
1.474TrpGln: 1.474 ± 0.041
0.811TrpArg: 0.811 ± 0.03
0.728TrpSer: 0.728 ± 0.026
0.453TrpThr: 0.453 ± 0.023
0.81TrpVal: 0.81 ± 0.03
0.223TrpTrp: 0.223 ± 0.016
0.367TrpTyr: 0.367 ± 0.02
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.295TyrAla: 2.295 ± 0.045
0.435TyrCys: 0.435 ± 0.019
1.485TyrAsp: 1.485 ± 0.044
1.231TyrGlu: 1.231 ± 0.036
1.313TyrPhe: 1.313 ± 0.035
2.137TyrGly: 2.137 ± 0.05
0.824TyrHis: 0.824 ± 0.03
1.47TyrIle: 1.47 ± 0.033
1.036TyrLys: 1.036 ± 0.032
3.569TyrLeu: 3.569 ± 0.061
0.579TyrMet: 0.579 ± 0.026
0.95TyrAsn: 0.95 ± 0.033
1.433TyrPro: 1.433 ± 0.038
2.388TyrGln: 2.388 ± 0.048
1.74TyrArg: 1.74 ± 0.042
1.87TyrSer: 1.87 ± 0.037
1.207TyrThr: 1.207 ± 0.04
1.642TyrVal: 1.642 ± 0.041
0.463TyrTrp: 0.463 ± 0.022
0.919TyrTyr: 0.919 ± 0.029
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 3378 proteins (1121467 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski