Catenulispora acidiphila (strain DSM 44928 / JCM 14897 / NBRC 102108 / NRRL B-24433 / ID139908)
Average proteome isoelectric point is 6.12
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 8909 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|C7QIW6|C7QIW6_CATAD dTDP-glucose 4 6-dehydratase OS=Catenulispora acidiphila (strain DSM 44928 / JCM 14897 / NBRC 102108 / NRRL B-24433 / ID139908) OX=479433 GN=Caci_8193 PE=3 SV=1
MM1 pKa = 7.6 AGAAALTALSCAMLSGGPAAASVLPAPYY29 pKa = 10.58 GDD31 pKa = 4.81 LNADD35 pKa = 3.6 GNIDD39 pKa = 4.06 LLSVSSTGALQYY51 pKa = 9.7 WAGSGTGSVASPVAEE66 pKa = 4.24 PSAGDD71 pKa = 3.38 FTGDD75 pKa = 3.56 VIEE78 pKa = 4.79 GAGNFNRR85 pKa = 11.84 SSYY88 pKa = 10.92 QSLFLYY94 pKa = 10.79 SSATDD99 pKa = 3.28 HH100 pKa = 7.18 AYY102 pKa = 10.82 VEE104 pKa = 5.07 LGDD107 pKa = 3.73 NTGQFSTANEE117 pKa = 4.18 VVVPPPSGEE126 pKa = 3.97 ASWPVITQLVSPGDD140 pKa = 3.53 ITGHH144 pKa = 5.5 NRR146 pKa = 11.84 ADD148 pKa = 3.31 LVARR152 pKa = 11.84 VGDD155 pKa = 3.73 QLEE158 pKa = 4.56 VIPNVALGHH167 pKa = 5.87 YY168 pKa = 7.11 GAPVAVAGSGWSGRR182 pKa = 11.84 TVIGVVDD189 pKa = 3.63 ATGDD193 pKa = 3.92 GIKK196 pKa = 10.7 DD197 pKa = 3.97 LIARR201 pKa = 11.84 DD202 pKa = 3.83 DD203 pKa = 3.63 ATGVVWLYY211 pKa = 10.8 QGVAGGTFGDD221 pKa = 3.76 EE222 pKa = 4.14 TTRR225 pKa = 11.84 VQIGTGLDD233 pKa = 3.16 AADD236 pKa = 3.74 YY237 pKa = 10.06 PFVITKK243 pKa = 10.44 GDD245 pKa = 3.68 ADD247 pKa = 4.22 GDD249 pKa = 3.95 GHH251 pKa = 8.18 ADD253 pKa = 3.45 VYY255 pKa = 11.61 AVGASGGLYY264 pKa = 10.34 LSAGNAGGGFGAPTLVSSDD283 pKa = 3.99 PAWTGITALGG293 pKa = 3.56
Molecular weight: 29.03 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.711
IPC2_protein 3.783
IPC_protein 3.808
Toseland 3.579
ProMoST 3.999
Dawson 3.821
Bjellqvist 3.973
Wikipedia 3.783
Rodwell 3.63
Grimsley 3.478
Solomon 3.808
Lehninger 3.77
Nozaki 3.935
DTASelect 4.228
Thurlkill 3.643
EMBOSS 3.795
Sillero 3.935
Patrickios 0.807
IPC_peptide 3.808
IPC2_peptide 3.91
IPC2.peptide.svr19 3.816
Protein with the highest isoelectric point:
>tr|C7Q0H6|C7Q0H6_CATAD Uncharacterized protein OS=Catenulispora acidiphila (strain DSM 44928 / JCM 14897 / NBRC 102108 / NRRL B-24433 / ID139908) OX=479433 GN=Caci_8694 PE=4 SV=1
MM1 pKa = 7.56 WARR4 pKa = 11.84 ALKK7 pKa = 10.28 RR8 pKa = 11.84 LAALSIPVRR17 pKa = 11.84 MLRR20 pKa = 11.84 PVWFFTMRR28 pKa = 11.84 FLPKK32 pKa = 9.86 RR33 pKa = 11.84 FRR35 pKa = 11.84 PMWFLPTRR43 pKa = 11.84 FLPRR47 pKa = 11.84 LAAGG51 pKa = 3.73
Molecular weight: 6.22 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.506
IPC2_protein 11.14
IPC_protein 12.749
Toseland 12.91
ProMoST 13.408
Dawson 12.91
Bjellqvist 12.91
Wikipedia 13.378
Rodwell 12.501
Grimsley 12.939
Solomon 13.408
Lehninger 13.305
Nozaki 12.91
DTASelect 12.91
Thurlkill 12.91
EMBOSS 13.408
Sillero 12.91
Patrickios 12.237
IPC_peptide 13.408
IPC2_peptide 12.398
IPC2.peptide.svr19 9.157
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
8909
0
8909
3085666
29
7149
346.4
36.84
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
14.097 ± 0.04
0.751 ± 0.007
5.88 ± 0.022
5.123 ± 0.03
2.895 ± 0.016
9.275 ± 0.027
2.176 ± 0.014
3.6 ± 0.015
2.1 ± 0.018
9.82 ± 0.034
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
1.831 ± 0.01
2.163 ± 0.019
5.909 ± 0.022
3.092 ± 0.017
6.98 ± 0.037
5.854 ± 0.027
6.46 ± 0.033
8.286 ± 0.021
1.528 ± 0.011
2.18 ± 0.015
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here