Coriobacterium glomerans (strain ATCC 49209 / DSM 20642 / JCM 10262 / PW2)
Average proteome isoelectric point is 6.13
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 1768 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|F2N9R7|F2N9R7_CORGP Uncharacterized protein OS=Coriobacterium glomerans (strain ATCC 49209 / DSM 20642 / JCM 10262 / PW2) OX=700015 GN=Corgl_1062 PE=3 SV=1
MM1 pKa = 7.53 ALIDD5 pKa = 4.31 PVLIDD10 pKa = 3.65 LTGKK14 pKa = 10.67 AEE16 pKa = 4.27 GPGEE20 pKa = 4.15 SFTFSGHH27 pKa = 6.06 AAASGYY33 pKa = 11.39 DD34 pKa = 3.05 MGEE37 pKa = 3.67 RR38 pKa = 11.84 HH39 pKa = 6.81 LEE41 pKa = 4.21 LEE43 pKa = 5.03 DD44 pKa = 3.81 GLSFEE49 pKa = 4.94 VAFTNTGDD57 pKa = 4.34 GILLTGMVRR66 pKa = 11.84 GRR68 pKa = 11.84 AAGVCDD74 pKa = 3.37 RR75 pKa = 11.84 CLDD78 pKa = 3.51 VARR81 pKa = 11.84 IDD83 pKa = 3.16 IAGAIEE89 pKa = 4.39 EE90 pKa = 4.76 YY91 pKa = 10.89 YY92 pKa = 11.08 LLEE95 pKa = 4.73 KK96 pKa = 10.46 PHH98 pKa = 7.4 DD99 pKa = 3.91 PTCYY103 pKa = 9.92 EE104 pKa = 4.99 DD105 pKa = 4.68 GFEE108 pKa = 4.61 LLGSDD113 pKa = 4.63 RR114 pKa = 11.84 VVDD117 pKa = 4.5 LAAPLSDD124 pKa = 4.95 AVITDD129 pKa = 3.52 TPFALLCSPDD139 pKa = 3.74 CAGLCPVCGCNLNHH153 pKa = 7.4 AACDD157 pKa = 3.73 CMTDD161 pKa = 3.15 SFDD164 pKa = 3.42 VARR167 pKa = 11.84 SGEE170 pKa = 4.17 EE171 pKa = 3.95 SPFAALRR178 pKa = 11.84 DD179 pKa = 3.94 LEE181 pKa = 4.66 IEE183 pKa = 4.12
Molecular weight: 19.37 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.799
IPC2_protein 3.948
IPC_protein 3.935
Toseland 3.732
ProMoST 4.062
Dawson 3.923
Bjellqvist 4.101
Wikipedia 3.834
Rodwell 3.77
Grimsley 3.643
Solomon 3.91
Lehninger 3.872
Nozaki 4.037
DTASelect 4.253
Thurlkill 3.783
EMBOSS 3.846
Sillero 4.062
Patrickios 0.693
IPC_peptide 3.91
IPC2_peptide 4.037
IPC2.peptide.svr19 3.944
Protein with the highest isoelectric point:
>tr|F2N9H1|F2N9H1_CORGP Ribosome-recycling factor OS=Coriobacterium glomerans (strain ATCC 49209 / DSM 20642 / JCM 10262 / PW2) OX=700015 GN=frr PE=3 SV=1
MM1 pKa = 7.35 KK2 pKa = 9.42 RR3 pKa = 11.84 TYY5 pKa = 10.34 QPNKK9 pKa = 8.64 RR10 pKa = 11.84 KK11 pKa = 9.51 RR12 pKa = 11.84 AKK14 pKa = 9.92 SHH16 pKa = 5.28 GFRR19 pKa = 11.84 ARR21 pKa = 11.84 MATKK25 pKa = 10.27 GGRR28 pKa = 11.84 AVLARR33 pKa = 11.84 RR34 pKa = 11.84 RR35 pKa = 11.84 AKK37 pKa = 9.9 GRR39 pKa = 11.84 RR40 pKa = 11.84 RR41 pKa = 11.84 LTVV44 pKa = 2.76
Molecular weight: 5.18 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.449
IPC2_protein 11.155
IPC_protein 12.544
Toseland 12.705
ProMoST 13.203
Dawson 12.705
Bjellqvist 12.705
Wikipedia 13.188
Rodwell 12.398
Grimsley 12.749
Solomon 13.203
Lehninger 13.1
Nozaki 12.705
DTASelect 12.705
Thurlkill 12.705
EMBOSS 13.203
Sillero 12.705
Patrickios 12.135
IPC_peptide 13.203
IPC2_peptide 12.193
IPC2.peptide.svr19 9.082
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
1768
0
1768
613803
30
1560
347.2
37.86
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
11.264 ± 0.077
1.596 ± 0.025
6.286 ± 0.054
6.032 ± 0.06
3.662 ± 0.04
8.109 ± 0.048
1.986 ± 0.027
6.115 ± 0.049
3.156 ± 0.046
9.414 ± 0.062
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.664 ± 0.028
2.559 ± 0.034
4.209 ± 0.031
2.76 ± 0.031
7.313 ± 0.064
6.7 ± 0.049
5.141 ± 0.039
7.434 ± 0.049
1.03 ± 0.021
2.569 ± 0.03
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here