Sphingomonas sanxanigenens DSM 19645 = NX02

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Alphaproteobacteria; Sphingomonadales; Sphingomonadaceae; Sphingomonas; Sphingomonas sanxanigenens

Average proteome isoelectric point is 6.59

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 6101 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|W0AHC5|W0AHC5_9SPHN Uncharacterized protein OS=Sphingomonas sanxanigenens DSM 19645 = NX02 OX=1123269 GN=NX02_24445 PE=3 SV=1
MM1 pKa = 7.42FSLLSVYY8 pKa = 10.26RR9 pKa = 11.84APQVTLTAEE18 pKa = 4.15GHH20 pKa = 5.94PMFVYY25 pKa = 10.4AKK27 pKa = 8.7PCSGEE32 pKa = 4.11VIMTEE37 pKa = 3.85SGKK40 pKa = 10.16FDD42 pKa = 3.71YY43 pKa = 10.8FFSDD47 pKa = 4.31DD48 pKa = 4.56DD49 pKa = 4.28APSDD53 pKa = 3.74EE54 pKa = 5.39DD55 pKa = 3.84VAFDD59 pKa = 4.54GLEE62 pKa = 4.11LPPALVDD69 pKa = 3.79YY70 pKa = 10.68ISTYY74 pKa = 10.37GAA76 pKa = 3.67

Molecular weight: 8.34 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|W0A5G0|W0A5G0_9SPHN Transcriptional regulator OS=Sphingomonas sanxanigenens DSM 19645 = NX02 OX=1123269 GN=NX02_17920 PE=3 SV=1
MM1 pKa = 7.45KK2 pKa = 9.59RR3 pKa = 11.84TFQPSKK9 pKa = 9.58LVRR12 pKa = 11.84ARR14 pKa = 11.84RR15 pKa = 11.84HH16 pKa = 4.61GFRR19 pKa = 11.84SRR21 pKa = 11.84SATVGGRR28 pKa = 11.84KK29 pKa = 8.17VLRR32 pKa = 11.84ARR34 pKa = 11.84RR35 pKa = 11.84ARR37 pKa = 11.84GRR39 pKa = 11.84NKK41 pKa = 10.45LSAA44 pKa = 3.94

Molecular weight: 5.08 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

6101

0

6101

1922014

29

5012

315.0

33.95

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

13.922 ± 0.05

0.748 ± 0.011

6.154 ± 0.028

5.161 ± 0.03

3.522 ± 0.017

9.148 ± 0.05

1.983 ± 0.018

5.033 ± 0.019

2.537 ± 0.024

9.827 ± 0.039

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.334 ± 0.017

2.392 ± 0.023

5.423 ± 0.029

2.926 ± 0.017

7.795 ± 0.041

4.972 ± 0.025

5.315 ± 0.033

7.129 ± 0.025

1.459 ± 0.016

2.22 ± 0.02
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski