Amino acid dipepetide frequency for Streptomyces autolyticus

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10^-3.

For more information see sequence space article on Wikipedia.

	Ala	Cys	Asp	Glu	Phe	Gly	His	Ile	Lys	Leu	Met	Asn	Pro	Gln	Arg	Ser	Thr	Val	Trp	Tyr	Xaa
Ala	21.223AlaAla: 21.223 ± 0.139	1.064AlaCys: 1.064 ± 0.024	8.457AlaAsp: 8.457 ± 0.069	9.131AlaGlu: 9.131 ± 0.086	3.548AlaPhe: 3.548 ± 0.044	12.909AlaGly: 12.909 ± 0.106	3.005AlaHis: 3.005 ± 0.037	3.774AlaIle: 3.774 ± 0.046	2.677AlaLys: 2.677 ± 0.045	14.724AlaLeu: 14.724 ± 0.103	2.585AlaMet: 2.585 ± 0.033	1.877AlaAsn: 1.877 ± 0.026	7.181AlaPro: 7.181 ± 0.076	3.632AlaGln: 3.632 ± 0.039	10.233AlaArg: 10.233 ± 0.068	5.812AlaSer: 5.812 ± 0.045	7.213AlaThr: 7.213 ± 0.061	12.234AlaVal: 12.234 ± 0.091	1.82AlaTrp: 1.82 ± 0.027	2.57AlaTyr: 2.57 ± 0.033	0.0AlaXaa: 0.0 ± 0.0
Cys	1.106CysAla: 1.106 ± 0.017	0.092CysCys: 0.092 ± 0.006	0.514CysAsp: 0.514 ± 0.016	0.426CysGlu: 0.426 ± 0.011	0.206CysPhe: 0.206 ± 0.009	0.946CysGly: 0.946 ± 0.024	0.188CysHis: 0.188 ± 0.009	0.135CysIle: 0.135 ± 0.007	0.12CysLys: 0.12 ± 0.007	0.712CysLeu: 0.712 ± 0.017	0.118CysMet: 0.118 ± 0.006	0.125CysAsn: 0.125 ± 0.006	0.487CysPro: 0.487 ± 0.015	0.171CysGln: 0.171 ± 0.008	0.627CysArg: 0.627 ± 0.016	0.451CysSer: 0.451 ± 0.016	0.461CysThr: 0.461 ± 0.013	0.67CysVal: 0.67 ± 0.017	0.125CysTrp: 0.125 ± 0.007	0.143CysTyr: 0.143 ± 0.008	0.0CysXaa: 0.0 ± 0.0
Asp	7.745AspAla: 7.745 ± 0.075	0.394AspCys: 0.394 ± 0.013	3.497AspAsp: 3.497 ± 0.045	3.858AspGlu: 3.858 ± 0.042	1.613AspPhe: 1.613 ± 0.029	6.533AspGly: 6.533 ± 0.065	1.567AspHis: 1.567 ± 0.028	2.022AspIle: 2.022 ± 0.027	1.108AspLys: 1.108 ± 0.026	6.035AspLeu: 6.035 ± 0.053	0.795AspMet: 0.795 ± 0.017	0.917AspAsn: 0.917 ± 0.021	4.633AspPro: 4.633 ± 0.043	1.629AspGln: 1.629 ± 0.026	5.095AspArg: 5.095 ± 0.052	2.491AspSer: 2.491 ± 0.028	3.34AspThr: 3.34 ± 0.04	4.375AspVal: 4.375 ± 0.037	1.057AspTrp: 1.057 ± 0.02	1.175AspTyr: 1.175 ± 0.02	0.0AspXaa: 0.0 ± 0.0
Glu	7.516GluAla: 7.516 ± 0.077	0.408GluCys: 0.408 ± 0.012	2.878GluAsp: 2.878 ± 0.042	3.676GluGlu: 3.676 ± 0.046	1.472GluPhe: 1.472 ± 0.026	4.557GluGly: 4.557 ± 0.039	1.528GluHis: 1.528 ± 0.02	2.23GluIle: 2.23 ± 0.032	1.328GluLys: 1.328 ± 0.027	7.036GluLeu: 7.036 ± 0.063	0.879GluMet: 0.879 ± 0.017	0.969GluAsn: 0.969 ± 0.02	3.594GluPro: 3.594 ± 0.047	2.028GluGln: 2.028 ± 0.028	5.995GluArg: 5.995 ± 0.052	2.66GluSer: 2.66 ± 0.028	2.961GluThr: 2.961 ± 0.035	4.576GluVal: 4.576 ± 0.045	0.772GluTrp: 0.772 ± 0.017	1.025GluTyr: 1.025 ± 0.02	0.0GluXaa: 0.0 ± 0.0
Phe	3.606PheAla: 3.606 ± 0.037	0.248PheCys: 0.248 ± 0.01	2.034PheAsp: 2.034 ± 0.028	1.428PheGlu: 1.428 ± 0.02	0.842PhePhe: 0.842 ± 0.019	3.023PheGly: 3.023 ± 0.036	0.635PheHis: 0.635 ± 0.014	0.839PheIle: 0.839 ± 0.018	0.468PheLys: 0.468 ± 0.013	2.54PheLeu: 2.54 ± 0.029	0.384PheMet: 0.384 ± 0.011	0.537PheAsn: 0.537 ± 0.017	1.421PhePro: 1.421 ± 0.024	0.716PheGln: 0.716 ± 0.014	1.805PheArg: 1.805 ± 0.028	1.479PheSer: 1.479 ± 0.022	1.942PheThr: 1.942 ± 0.029	2.079PheVal: 2.079 ± 0.03	0.417PheTrp: 0.417 ± 0.013	0.547PheTyr: 0.547 ± 0.014	0.0PheXaa: 0.0 ± 0.0
Gly	11.717GlyAla: 11.717 ± 0.099	0.858GlyCys: 0.858 ± 0.021	5.206GlyAsp: 5.206 ± 0.055	5.318GlyGlu: 5.318 ± 0.05	2.87GlyPhe: 2.87 ± 0.034	9.604GlyGly: 9.604 ± 0.093	2.579GlyHis: 2.579 ± 0.037	3.437GlyIle: 3.437 ± 0.036	2.363GlyLys: 2.363 ± 0.035	9.496GlyLeu: 9.496 ± 0.075	2.115GlyMet: 2.115 ± 0.03	1.674GlyAsn: 1.674 ± 0.03	5.618GlyPro: 5.618 ± 0.062	2.791GlyGln: 2.791 ± 0.043	8.07GlyArg: 8.07 ± 0.058	5.384GlySer: 5.384 ± 0.047	6.203GlyThr: 6.203 ± 0.051	7.803GlyVal: 7.803 ± 0.08	1.685GlyTrp: 1.685 ± 0.026	2.294GlyTyr: 2.294 ± 0.029	0.0GlyXaa: 0.0 ± 0.0
His	2.615HisAla: 2.615 ± 0.033	0.207HisCys: 0.207 ± 0.01	1.359HisAsp: 1.359 ± 0.022	1.238HisGlu: 1.238 ± 0.023	0.647HisPhe: 0.647 ± 0.016	2.54HisGly: 2.54 ± 0.031	0.733HisHis: 0.733 ± 0.017	0.817HisIle: 0.817 ± 0.018	0.348HisLys: 0.348 ± 0.011	2.465HisLeu: 2.465 ± 0.03	0.361HisMet: 0.361 ± 0.011	0.354HisAsn: 0.354 ± 0.011	1.995HisPro: 1.995 ± 0.03	0.727HisGln: 0.727 ± 0.017	2.152HisArg: 2.152 ± 0.03	1.096HisSer: 1.096 ± 0.023	1.53HisThr: 1.53 ± 0.028	1.581HisVal: 1.581 ± 0.027	0.382HisTrp: 0.382 ± 0.011	0.484HisTyr: 0.484 ± 0.013	0.0HisXaa: 0.0 ± 0.0
Ile	5.096IleAla: 5.096 ± 0.053	0.307IleCys: 0.307 ± 0.011	2.358IleAsp: 2.358 ± 0.032	2.108IleGlu: 2.108 ± 0.03	0.727IlePhe: 0.727 ± 0.019	3.731IleGly: 3.731 ± 0.044	0.661IleHis: 0.661 ± 0.018	0.865IleIle: 0.865 ± 0.021	0.742IleLys: 0.742 ± 0.018	2.399IleLeu: 2.399 ± 0.039	0.456IleMet: 0.456 ± 0.014	0.704IleAsn: 0.704 ± 0.018	1.824IlePro: 1.824 ± 0.029	0.729IleGln: 0.729 ± 0.016	2.418IleArg: 2.418 ± 0.032	1.829IleSer: 1.829 ± 0.028	2.282IleThr: 2.282 ± 0.031	2.745IleVal: 2.745 ± 0.037	0.379IleTrp: 0.379 ± 0.011	0.516IleTyr: 0.516 ± 0.016	0.0IleXaa: 0.0 ± 0.0
Lys	2.718LysAla: 2.718 ± 0.039	0.103LysCys: 0.103 ± 0.007	1.249LysAsp: 1.249 ± 0.027	1.102LysGlu: 1.102 ± 0.023	0.399LysPhe: 0.399 ± 0.013	1.785LysGly: 1.785 ± 0.031	0.378LysHis: 0.378 ± 0.012	0.772LysIle: 0.772 ± 0.019	0.782LysLys: 0.782 ± 0.026	1.888LysLeu: 1.888 ± 0.028	0.368LysMet: 0.368 ± 0.011	0.477LysAsn: 0.477 ± 0.015	1.287LysPro: 1.287 ± 0.028	0.612LysGln: 0.612 ± 0.018	1.46LysArg: 1.46 ± 0.029	1.133LysSer: 1.133 ± 0.022	1.19LysThr: 1.19 ± 0.025	1.748LysVal: 1.748 ± 0.028	0.248LysTrp: 0.248 ± 0.009	0.393LysTyr: 0.393 ± 0.014	0.0LysXaa: 0.0 ± 0.0
Leu	14.83LeuAla: 14.83 ± 0.098	0.84LeuCys: 0.84 ± 0.022	6.739LeuAsp: 6.739 ± 0.054	4.967LeuGlu: 4.967 ± 0.049	2.623LeuPhe: 2.623 ± 0.036	9.276LeuGly: 9.276 ± 0.055	2.347LeuHis: 2.347 ± 0.03	3.583LeuIle: 3.583 ± 0.046	1.81LeuLys: 1.81 ± 0.028	11.066LeuLeu: 11.066 ± 0.083	1.624LeuMet: 1.624 ± 0.027	1.622LeuAsn: 1.622 ± 0.029	6.479LeuPro: 6.479 ± 0.059	1.899LeuGln: 1.899 ± 0.028	9.058LeuArg: 9.058 ± 0.063	5.562LeuSer: 5.562 ± 0.048	6.97LeuThr: 6.97 ± 0.056	8.431LeuVal: 8.431 ± 0.061	1.328LeuTrp: 1.328 ± 0.025	1.786LeuTyr: 1.786 ± 0.026	0.0LeuXaa: 0.0 ± 0.0
Met	2.435MetAla: 2.435 ± 0.032	0.133MetCys: 0.133 ± 0.008	0.966MetAsp: 0.966 ± 0.018	0.78MetGlu: 0.78 ± 0.018	0.447MetPhe: 0.447 ± 0.014	1.401MetGly: 1.401 ± 0.024	0.339MetHis: 0.339 ± 0.011	0.635MetIle: 0.635 ± 0.017	0.393MetLys: 0.393 ± 0.014	1.61MetLeu: 1.61 ± 0.027	0.286MetMet: 0.286 ± 0.01	0.412MetAsn: 0.412 ± 0.013	1.066MetPro: 1.066 ± 0.022	0.385MetGln: 0.385 ± 0.011	1.488MetArg: 1.488 ± 0.025	1.315MetSer: 1.315 ± 0.023	1.479MetThr: 1.479 ± 0.026	1.373MetVal: 1.373 ± 0.025	0.213MetTrp: 0.213 ± 0.009	0.317MetTyr: 0.317 ± 0.012	0.0MetXaa: 0.0 ± 0.0
Asn	2.196AsnAla: 2.196 ± 0.027	0.152AsnCys: 0.152 ± 0.008	0.905AsnAsp: 0.905 ± 0.021	0.781AsnGlu: 0.781 ± 0.017	0.454AsnPhe: 0.454 ± 0.014	1.946AsnGly: 1.946 ± 0.033	0.402AsnHis: 0.402 ± 0.012	0.628AsnIle: 0.628 ± 0.015	0.359AsnLys: 0.359 ± 0.013	1.61AsnLeu: 1.61 ± 0.028	0.28AsnMet: 0.28 ± 0.011	0.394AsnAsn: 0.394 ± 0.013	1.307AsnPro: 1.307 ± 0.028	0.51AsnGln: 0.51 ± 0.015	1.214AsnArg: 1.214 ± 0.024	0.862AsnSer: 0.862 ± 0.02	1.108AsnThr: 1.108 ± 0.026	1.277AsnVal: 1.277 ± 0.026	0.263AsnTrp: 0.263 ± 0.01	0.41AsnTyr: 0.41 ± 0.013	0.0AsnXaa: 0.0 ± 0.0
Pro	8.476ProAla: 8.476 ± 0.074	0.323ProCys: 0.323 ± 0.01	4.629ProAsp: 4.629 ± 0.046	4.677ProGlu: 4.677 ± 0.051	1.488ProPhe: 1.488 ± 0.027	6.952ProGly: 6.952 ± 0.069	1.431ProHis: 1.431 ± 0.027	1.452ProIle: 1.452 ± 0.023	1.174ProLys: 1.174 ± 0.023	5.494ProLeu: 5.494 ± 0.047	1.029ProMet: 1.029 ± 0.023	0.895ProAsn: 0.895 ± 0.019	3.714ProPro: 3.714 ± 0.072	1.627ProGln: 1.627 ± 0.033	4.26ProArg: 4.26 ± 0.04	3.294ProSer: 3.294 ± 0.039	3.321ProThr: 3.321 ± 0.046	5.288ProVal: 5.288 ± 0.045	0.935ProTrp: 0.935 ± 0.02	1.367ProTyr: 1.367 ± 0.028	0.0ProXaa: 0.0 ± 0.0
Gln	3.567GlnAla: 3.567 ± 0.045	0.16GlnCys: 0.16 ± 0.009	1.414GlnAsp: 1.414 ± 0.025	1.409GlnGlu: 1.409 ± 0.023	0.631GlnPhe: 0.631 ± 0.016	2.342GlnGly: 2.342 ± 0.037	0.627GlnHis: 0.627 ± 0.016	1.057GlnIle: 1.057 ± 0.024	0.537GlnLys: 0.537 ± 0.017	2.908GlnLeu: 2.908 ± 0.036	0.488GlnMet: 0.488 ± 0.014	0.483GlnAsn: 0.483 ± 0.018	1.681GlnPro: 1.681 ± 0.036	1.157GlnGln: 1.157 ± 0.032	2.492GlnArg: 2.492 ± 0.034	1.226GlnSer: 1.226 ± 0.02	1.253GlnThr: 1.253 ± 0.023	2.201GlnVal: 2.201 ± 0.028	0.433GlnTrp: 0.433 ± 0.011	0.529GlnTyr: 0.529 ± 0.017	0.0GlnXaa: 0.0 ± 0.0
Arg	10.011ArgAla: 10.011 ± 0.073	0.62ArgCys: 0.62 ± 0.014	4.366ArgAsp: 4.366 ± 0.053	4.87ArgGlu: 4.87 ± 0.049	2.389ArgPhe: 2.389 ± 0.03	6.321ArgGly: 6.321 ± 0.063	2.227ArgHis: 2.227 ± 0.03	3.344ArgIle: 3.344 ± 0.04	1.606ArgLys: 1.606 ± 0.028	9.178ArgLeu: 9.178 ± 0.069	1.785ArgMet: 1.785 ± 0.029	1.399ArgAsn: 1.399 ± 0.024	5.299ArgPro: 5.299 ± 0.052	2.38ArgGln: 2.38 ± 0.03	7.916ArgArg: 7.916 ± 0.07	4.091ArgSer: 4.091 ± 0.039	5.414ArgThr: 5.414 ± 0.054	5.883ArgVal: 5.883 ± 0.05	1.398ArgTrp: 1.398 ± 0.022	1.762ArgTyr: 1.762 ± 0.026	0.0ArgXaa: 0.0 ± 0.0
Ser	6.914SerAla: 6.914 ± 0.058	0.419SerCys: 0.419 ± 0.012	2.683SerAsp: 2.683 ± 0.034	2.437SerGlu: 2.437 ± 0.031	1.547SerPhe: 1.547 ± 0.023	6.389SerGly: 6.389 ± 0.05	1.072SerHis: 1.072 ± 0.021	1.469SerIle: 1.469 ± 0.024	0.97SerLys: 0.97 ± 0.021	4.922SerLeu: 4.922 ± 0.055	1.015SerMet: 1.015 ± 0.02	0.891SerAsn: 0.891 ± 0.019	3.241SerPro: 3.241 ± 0.039	1.265SerGln: 1.265 ± 0.02	3.699SerArg: 3.699 ± 0.038	3.024SerSer: 3.024 ± 0.045	2.954SerThr: 2.954 ± 0.034	4.229SerVal: 4.229 ± 0.044	0.926SerTrp: 0.926 ± 0.018	1.212SerTyr: 1.212 ± 0.024	0.0SerXaa: 0.0 ± 0.0
Thr	9.101ThrAla: 9.101 ± 0.066	0.396ThrCys: 0.396 ± 0.014	3.519ThrAsp: 3.519 ± 0.036	3.191ThrGlu: 3.191 ± 0.039	1.588ThrPhe: 1.588 ± 0.029	6.98ThrGly: 6.98 ± 0.064	1.231ThrHis: 1.231 ± 0.022	1.765ThrIle: 1.765 ± 0.03	1.073ThrLys: 1.073 ± 0.021	5.664ThrLeu: 5.664 ± 0.049	0.969ThrMet: 0.969 ± 0.021	0.977ThrAsn: 0.977 ± 0.018	4.094ThrPro: 4.094 ± 0.047	1.237ThrGln: 1.237 ± 0.023	3.851ThrArg: 3.851 ± 0.037	3.224ThrSer: 3.224 ± 0.037	3.777ThrThr: 3.777 ± 0.047	6.022ThrVal: 6.022 ± 0.05	0.844ThrTrp: 0.844 ± 0.018	1.26ThrTyr: 1.26 ± 0.023	0.0ThrXaa: 0.0 ± 0.0
Val	10.469ValAla: 10.469 ± 0.071	0.767ValCys: 0.767 ± 0.019	4.88ValAsp: 4.88 ± 0.052	4.738ValGlu: 4.738 ± 0.055	2.426ValPhe: 2.426 ± 0.033	6.436ValGly: 6.436 ± 0.053	1.927ValHis: 1.927 ± 0.031	3.03ValIle: 3.03 ± 0.039	1.551ValLys: 1.551 ± 0.027	9.249ValLeu: 9.249 ± 0.076	1.416ValMet: 1.416 ± 0.023	1.57ValAsn: 1.57 ± 0.025	5.036ValPro: 5.036 ± 0.047	1.915ValGln: 1.915 ± 0.028	7.252ValArg: 7.252 ± 0.06	4.465ValSer: 4.465 ± 0.047	5.245ValThr: 5.245 ± 0.055	7.659ValVal: 7.659 ± 0.065	1.166ValTrp: 1.166 ± 0.022	1.467ValTyr: 1.467 ± 0.028	0.0ValXaa: 0.0 ± 0.0
Trp	1.642TrpAla: 1.642 ± 0.026	0.169TrpCys: 0.169 ± 0.008	0.825TrpAsp: 0.825 ± 0.02	0.767TrpGlu: 0.767 ± 0.019	0.472TrpPhe: 0.472 ± 0.013	1.14TrpGly: 1.14 ± 0.023	0.364TrpHis: 0.364 ± 0.012	0.523TrpIle: 0.523 ± 0.015	0.335TrpLys: 0.335 ± 0.012	1.805TrpLeu: 1.805 ± 0.028	0.284TrpMet: 0.284 ± 0.01	0.4TrpAsn: 0.4 ± 0.012	0.794TrpPro: 0.794 ± 0.018	0.578TrpGln: 0.578 ± 0.016	1.403TrpArg: 1.403 ± 0.024	0.968TrpSer: 0.968 ± 0.021	0.991TrpThr: 0.991 ± 0.02	0.963TrpVal: 0.963 ± 0.02	0.309TrpTrp: 0.309 ± 0.012	0.345TrpTyr: 0.345 ± 0.013	0.0TrpXaa: 0.0 ± 0.0
Tyr	2.687TyrAla: 2.687 ± 0.032	0.163TyrCys: 0.163 ± 0.008	1.402TyrAsp: 1.402 ± 0.026	1.187TyrGlu: 1.187 ± 0.022	0.601TyrPhe: 0.601 ± 0.014	2.274TyrGly: 2.274 ± 0.031	0.389TyrHis: 0.389 ± 0.013	0.499TyrIle: 0.499 ± 0.015	0.349TyrLys: 0.349 ± 0.012	2.041TyrLeu: 2.041 ± 0.035	0.233TyrMet: 0.233 ± 0.01	0.379TyrAsn: 0.379 ± 0.012	1.076TyrPro: 1.076 ± 0.021	0.608TyrGln: 0.608 ± 0.014	1.774TyrArg: 1.774 ± 0.028	0.913TyrSer: 0.913 ± 0.02	1.127TyrThr: 1.127 ± 0.021	1.526TyrVal: 1.526 ± 0.025	0.375TyrTrp: 0.375 ± 0.013	0.437TyrTyr: 0.437 ± 0.015	0.0TyrXaa: 0.0 ± 0.0
Xaa	0.0XaaAla: 0.0 ± 0.0	0.0XaaCys: 0.0 ± 0.0	0.0XaaAsp: 0.0 ± 0.0	0.0XaaGlu: 0.0 ± 0.0	0.0XaaPhe: 0.0 ± 0.0	0.0XaaGly: 0.0 ± 0.0	0.0XaaHis: 0.0 ± 0.0	0.0XaaIle: 0.0 ± 0.0	0.0XaaLys: 0.0 ± 0.0	0.0XaaLeu: 0.0 ± 0.0	0.0XaaMet: 0.0 ± 0.0	0.0XaaAsn: 0.0 ± 0.0	0.0XaaPro: 0.0 ± 0.0	0.0XaaGln: 0.0 ± 0.0	0.0XaaArg: 0.0 ± 0.0	0.0XaaSer: 0.0 ± 0.0	0.0XaaThr: 0.0 ± 0.0	0.0XaaVal: 0.0 ± 0.0	0.0XaaTrp: 0.0 ± 0.0	0.0XaaTyr: 0.0 ± 0.0	0.0XaaXaa: 0.0 ± 0.0

Statistics based on 7686 proteins (2766839 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944

Contact: Lukasz P. Kozlowski

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