Vibrio phage phiV039C

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Myoviridae; unclassified Myoviridae

Average proteome isoelectric point is 5.88

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 36 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A6M3T8F1|A0A6M3T8F1_9CAUD Uncharacterized protein OS=Vibrio phage phiV039C OX=2705110 PE=4 SV=1
MM1 pKa = 7.0STIITADD8 pKa = 3.15GGNISSDD15 pKa = 3.23RR16 pKa = 11.84YY17 pKa = 9.82VEE19 pKa = 4.41IISGVGGAAAFPTRR33 pKa = 11.84QLILRR38 pKa = 11.84LFTNNEE44 pKa = 3.87AFPTNSVVTFSSPEE58 pKa = 3.81DD59 pKa = 3.34LLTYY63 pKa = 10.95LNGDD67 pKa = 3.75STAEE71 pKa = 4.03EE72 pKa = 4.47YY73 pKa = 10.5KK74 pKa = 10.06QAVYY78 pKa = 10.45YY79 pKa = 9.9FSYY82 pKa = 9.92ISKK85 pKa = 10.62AIRR88 pKa = 11.84TPKK91 pKa = 9.74QLSIARR97 pKa = 11.84WAEE100 pKa = 4.11VNTSAQVFGSKK111 pKa = 10.19AATLDD116 pKa = 3.3QLKK119 pKa = 9.37TYY121 pKa = 7.58TTATLDD127 pKa = 3.3VTLNGTTYY135 pKa = 10.93NATAIDD141 pKa = 4.37LSLAASFADD150 pKa = 3.97VATTLQGKK158 pKa = 9.6ISALDD163 pKa = 3.56ASLTAATVTYY173 pKa = 10.29NSASGGFDD181 pKa = 2.98VDD183 pKa = 4.06TNGDD187 pKa = 3.42ADD189 pKa = 3.91GALTFASATAGFLSDD204 pKa = 4.16IGLDD208 pKa = 3.45STAVFSSGIAEE219 pKa = 4.08QTLTEE224 pKa = 4.56LMTSSTNLSNNFGSLAFVQDD244 pKa = 4.06LSLTEE249 pKa = 4.02IEE251 pKa = 6.22EE252 pKa = 4.14VATWNKK258 pKa = 8.91GQNFAYY264 pKa = 8.94MYY266 pKa = 10.08CEE268 pKa = 4.26KK269 pKa = 9.97STKK272 pKa = 10.42ANSQSYY278 pKa = 9.61FDD280 pKa = 3.78TLKK283 pKa = 10.94GYY285 pKa = 10.83GGLAVTLYY293 pKa = 10.44DD294 pKa = 4.66DD295 pKa = 4.45SVADD299 pKa = 4.48EE300 pKa = 4.71YY301 pKa = 11.1PWLHH305 pKa = 6.63PAAEE309 pKa = 4.2TAAIDD314 pKa = 3.85PSKK317 pKa = 11.08PNAFPNYY324 pKa = 8.79MFAPDD329 pKa = 3.66SALSAVVTTDD339 pKa = 4.09SEE341 pKa = 4.87ADD343 pKa = 3.27TYY345 pKa = 10.98DD346 pKa = 3.58ARR348 pKa = 11.84RR349 pKa = 11.84MNYY352 pKa = 8.76MGRR355 pKa = 11.84TQEE358 pKa = 4.06AGQTRR363 pKa = 11.84TWYY366 pKa = 9.93QRR368 pKa = 11.84GVLMGGDD375 pKa = 3.57TDD377 pKa = 3.69ATTMSVYY384 pKa = 10.04MGEE387 pKa = 4.07AWLKK391 pKa = 11.26AEE393 pKa = 5.35LKK395 pKa = 10.64SQFLNMFNALPGITPDD411 pKa = 2.95IAGRR415 pKa = 11.84SYY417 pKa = 11.31INLYY421 pKa = 10.38LDD423 pKa = 3.67AAVAQALPDD432 pKa = 4.06GGNGMISVGKK442 pKa = 10.0QLTTTQQAYY451 pKa = 6.65ITQVSGDD458 pKa = 3.43EE459 pKa = 4.35DD460 pKa = 2.96AWKK463 pKa = 9.99QVQSEE468 pKa = 5.23GYY470 pKa = 8.11WYY472 pKa = 9.21TVNFFSTIAEE482 pKa = 4.18SGVTEE487 pKa = 4.22WTAEE491 pKa = 3.84YY492 pKa = 10.1TLIYY496 pKa = 9.42ATAEE500 pKa = 4.17KK501 pKa = 10.05VRR503 pKa = 11.84KK504 pKa = 9.94VSGTHH509 pKa = 5.91ILII512 pKa = 4.45

Molecular weight: 55.11 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A6M3T8H9|A0A6M3T8H9_9CAUD Uncharacterized protein OS=Vibrio phage phiV039C OX=2705110 PE=4 SV=1
MM1 pKa = 6.94ITPRR5 pKa = 11.84QLQVLRR11 pKa = 11.84LYY13 pKa = 11.36ANGLTSIEE21 pKa = 4.07IAAEE25 pKa = 3.68LAISVRR31 pKa = 11.84TVEE34 pKa = 3.9EE35 pKa = 3.86HH36 pKa = 7.01LRR38 pKa = 11.84GCRR41 pKa = 11.84VEE43 pKa = 5.74LKK45 pKa = 10.64AKK47 pKa = 10.45NSVHH51 pKa = 6.26AVALAMQKK59 pKa = 10.86GIIKK63 pKa = 10.06LIILLSVYY71 pKa = 10.4QGMAADD77 pKa = 3.54YY78 pKa = 9.85HH79 pKa = 7.13EE80 pKa = 5.11DD81 pKa = 3.24MRR83 pKa = 11.84RR84 pKa = 11.84PPQVRR89 pKa = 11.84VRR91 pKa = 11.84ITRR94 pKa = 11.84TQRR97 pKa = 11.84RR98 pKa = 11.84EE99 pKa = 3.83CC100 pKa = 4.0

Molecular weight: 11.41 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

36

0

36

8952

72

812

248.7

27.74

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.764 ± 0.566

1.117 ± 0.167

6.49 ± 0.286

7.194 ± 0.656

3.943 ± 0.271

7.149 ± 0.396

1.597 ± 0.236

6.133 ± 0.282

6.591 ± 0.521

7.373 ± 0.289

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.659 ± 0.19

5.496 ± 0.281

3.664 ± 0.267

4.144 ± 0.367

4.457 ± 0.386

7.283 ± 0.534

5.831 ± 0.434

6.311 ± 0.343

1.408 ± 0.15

3.396 ± 0.319
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski