CRESS virus sp. ctBNR11

Taxonomy: Viruses; Monodnaviria; Shotokuvirae; Cressdnaviricota; unclassified Cressdnaviricota; CRESS viruses

Average proteome isoelectric point is 8.52

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A5Q2W8D9|A0A5Q2W8D9_9VIRU Rep protein OS=CRESS virus sp. ctBNR11 OX=2656677 PE=4 SV=1
MM1 pKa = 7.32AAEE4 pKa = 4.32TEE6 pKa = 4.54GTNGTVEE13 pKa = 4.48NIGNRR18 pKa = 11.84NKK20 pKa = 10.12VLNQRR25 pKa = 11.84NRR27 pKa = 11.84SRR29 pKa = 11.84AWCFTKK35 pKa = 10.81DD36 pKa = 3.17NATKK40 pKa = 10.42EE41 pKa = 4.11DD42 pKa = 3.44VGTFIKK48 pKa = 10.67YY49 pKa = 9.09FQLEE53 pKa = 4.13DD54 pKa = 3.57MDD56 pKa = 5.68YY57 pKa = 9.55MFQMEE62 pKa = 4.53KK63 pKa = 10.96GEE65 pKa = 4.17KK66 pKa = 9.11TGMIHH71 pKa = 6.5LQGVCRR77 pKa = 11.84FKK79 pKa = 11.02NPRR82 pKa = 11.84DD83 pKa = 3.12RR84 pKa = 11.84WPEE87 pKa = 3.78LKK89 pKa = 10.73AHH91 pKa = 6.88WDD93 pKa = 3.47RR94 pKa = 11.84CRR96 pKa = 11.84SWKK99 pKa = 10.31KK100 pKa = 10.71SIIYY104 pKa = 9.7CSKK107 pKa = 8.49TDD109 pKa = 3.46TRR111 pKa = 11.84IEE113 pKa = 4.34GPWTNLDD120 pKa = 3.3IKK122 pKa = 11.03VKK124 pKa = 10.34EE125 pKa = 4.18KK126 pKa = 10.47PQDD129 pKa = 3.58YY130 pKa = 10.98LKK132 pKa = 11.06DD133 pKa = 3.47KK134 pKa = 10.58EE135 pKa = 4.87LYY137 pKa = 9.55PFQAEE142 pKa = 3.88ILMMLGGKK150 pKa = 9.72IMDD153 pKa = 3.97RR154 pKa = 11.84TIFWYY159 pKa = 10.39FDD161 pKa = 3.32EE162 pKa = 5.27KK163 pKa = 11.56GNTGKK168 pKa = 8.5STLVRR173 pKa = 11.84HH174 pKa = 6.24ILLNNQAICRR184 pKa = 11.84SGSGKK189 pKa = 10.18DD190 pKa = 3.46CYY192 pKa = 10.62CALKK196 pKa = 10.49LRR198 pKa = 11.84CEE200 pKa = 4.44DD201 pKa = 3.32NKK203 pKa = 10.9EE204 pKa = 3.92PNIVIFDD211 pKa = 3.87LTRR214 pKa = 11.84DD215 pKa = 3.71EE216 pKa = 4.71EE217 pKa = 4.47NKK219 pKa = 10.42CNYY222 pKa = 9.7KK223 pKa = 10.65VIEE226 pKa = 4.45TIKK229 pKa = 10.94NGVFFSGKK237 pKa = 9.99YY238 pKa = 7.59EE239 pKa = 4.22SKK241 pKa = 10.72DD242 pKa = 3.23IIINPPHH249 pKa = 6.92VIVFSNWKK257 pKa = 9.44PKK259 pKa = 9.6IDD261 pKa = 4.2KK262 pKa = 10.22LSKK265 pKa = 10.37DD266 pKa = 2.8RR267 pKa = 11.84WSIHH271 pKa = 5.56EE272 pKa = 3.96IKK274 pKa = 10.69RR275 pKa = 11.84PDD277 pKa = 3.47FSSYY281 pKa = 10.46PIYY284 pKa = 10.3IEE286 pKa = 4.24NMM288 pKa = 3.4

Molecular weight: 33.97 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A5Q2W8D9|A0A5Q2W8D9_9VIRU Rep protein OS=CRESS virus sp. ctBNR11 OX=2656677 PE=4 SV=1
MM1 pKa = 6.85YY2 pKa = 10.08RR3 pKa = 11.84RR4 pKa = 11.84RR5 pKa = 11.84YY6 pKa = 8.98SRR8 pKa = 11.84ARR10 pKa = 11.84RR11 pKa = 11.84VRR13 pKa = 11.84RR14 pKa = 11.84RR15 pKa = 11.84FMNKK19 pKa = 7.64WGAKK23 pKa = 8.7SGRR26 pKa = 11.84TGWQKK31 pKa = 10.59FKK33 pKa = 10.96YY34 pKa = 10.07AAGKK38 pKa = 7.61TLNFASKK45 pKa = 10.08ALKK48 pKa = 9.6VAHH51 pKa = 5.81SVKK54 pKa = 10.73NLINVEE60 pKa = 4.17WKK62 pKa = 10.66RR63 pKa = 11.84KK64 pKa = 8.08DD65 pKa = 3.33TSSLGGAVGNVSPSTIGGMCVIAQGTDD92 pKa = 3.13YY93 pKa = 11.06NQRR96 pKa = 11.84NGNQVLLKK104 pKa = 10.58QFGLKK109 pKa = 8.45GTLLWNSNSTTQRR122 pKa = 11.84VRR124 pKa = 11.84IVIVMQKK131 pKa = 9.92NAEE134 pKa = 4.23DD135 pKa = 4.01PSTYY139 pKa = 9.61IDD141 pKa = 3.65MYY143 pKa = 11.32GAGLDD148 pKa = 3.41GFRR151 pKa = 11.84YY152 pKa = 10.31VEE154 pKa = 4.92DD155 pKa = 3.82KK156 pKa = 10.31PKK158 pKa = 10.91SRR160 pKa = 11.84IIFSRR165 pKa = 11.84LYY167 pKa = 10.44ILDD170 pKa = 4.05VNHH173 pKa = 6.51KK174 pKa = 9.61QYY176 pKa = 11.31NITFFKK182 pKa = 10.63KK183 pKa = 10.46FYY185 pKa = 7.53KK186 pKa = 9.37THH188 pKa = 6.01MKK190 pKa = 9.43WDD192 pKa = 4.45PNHH195 pKa = 6.01STTSPKK201 pKa = 10.79YY202 pKa = 9.98NDD204 pKa = 3.44FSVLARR210 pKa = 11.84SNDD213 pKa = 3.65DD214 pKa = 3.55DD215 pKa = 4.19NKK217 pKa = 9.42PTFVFNTRR225 pKa = 11.84VTYY228 pKa = 10.3IDD230 pKa = 3.32NN231 pKa = 4.01

Molecular weight: 26.72 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2

0

2

519

231

288

259.5

30.35

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

3.854 ± 0.876

1.927 ± 0.976

6.358 ± 0.477

4.624 ± 2.172

4.817 ± 0.247

6.166 ± 0.497

1.734 ± 0.002

6.936 ± 1.421

10.405 ± 0.575

5.588 ± 0.026

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.89 ± 0.191

7.514 ± 0.464

3.276 ± 0.443

2.89 ± 0.374

6.936 ± 0.842

5.973 ± 0.906

5.973 ± 0.623

5.202 ± 1.126

2.505 ± 0.222

4.432 ± 0.499
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski