Paraburkholderia phytofirmans OLGA172

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Betaproteobacteria; Burkholderiales; Burkholderiaceae; Paraburkholderia; Paraburkholderia phytofirmans

Average proteome isoelectric point is 6.68

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 7096 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A160FJN6|A0A160FJN6_9BURK EmrB/QacA subfamily drug resistance transporter OS=Paraburkholderia phytofirmans OLGA172 OX=1417228 GN=AYM40_09115 PE=4 SV=1
MM1 pKa = 7.27NLALNFDD8 pKa = 3.99WLTNLLAIIFVVACLYY24 pKa = 10.63DD25 pKa = 3.43SRR27 pKa = 11.84YY28 pKa = 10.69DD29 pKa = 3.54EE30 pKa = 4.96YY31 pKa = 11.52GTLTLSAAAMGLIVMAIEE49 pKa = 5.15LFLKK53 pKa = 10.33PAFDD57 pKa = 3.65MALL60 pKa = 3.35

Molecular weight: 6.69 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A160FPW7|A0A160FPW7_9BURK Uncharacterized protein OS=Paraburkholderia phytofirmans OLGA172 OX=1417228 GN=AYM40_20755 PE=4 SV=1
MM1 pKa = 6.64WQRR4 pKa = 11.84GSMHH8 pKa = 7.31DD9 pKa = 3.66PGGTAARR16 pKa = 11.84RR17 pKa = 11.84HH18 pKa = 6.05GGTAARR24 pKa = 11.84RR25 pKa = 11.84HH26 pKa = 6.05GGTAARR32 pKa = 11.84RR33 pKa = 11.84HH34 pKa = 6.05GGTAARR40 pKa = 11.84RR41 pKa = 11.84HH42 pKa = 6.05GGTAARR48 pKa = 11.84RR49 pKa = 11.84HH50 pKa = 6.05GGTAARR56 pKa = 11.84RR57 pKa = 11.84HH58 pKa = 6.05GGTAARR64 pKa = 11.84RR65 pKa = 11.84HH66 pKa = 6.05GGTAARR72 pKa = 11.84RR73 pKa = 11.84HH74 pKa = 6.05GGTAARR80 pKa = 11.84RR81 pKa = 11.84HH82 pKa = 6.05GGTAARR88 pKa = 11.84RR89 pKa = 11.84HH90 pKa = 6.05GGTAARR96 pKa = 11.84RR97 pKa = 11.84HH98 pKa = 6.05GGTAARR104 pKa = 11.84RR105 pKa = 11.84HH106 pKa = 5.53GGKK109 pKa = 9.84AARR112 pKa = 11.84RR113 pKa = 11.84QGGKK117 pKa = 9.37AARR120 pKa = 11.84RR121 pKa = 11.84QGGKK125 pKa = 9.37AARR128 pKa = 11.84RR129 pKa = 11.84QGGKK133 pKa = 9.37AARR136 pKa = 11.84RR137 pKa = 11.84QGGKK141 pKa = 9.33AARR144 pKa = 11.84RR145 pKa = 11.84QGAWTLGLAAARR157 pKa = 11.84SS158 pKa = 3.82

Molecular weight: 16.21 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

7096

0

7096

2227262

30

5046

313.9

34.16

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

12.318 ± 0.05

0.948 ± 0.009

5.41 ± 0.025

5.292 ± 0.026

3.72 ± 0.019

7.984 ± 0.029

2.334 ± 0.014

4.788 ± 0.019

3.286 ± 0.026

10.161 ± 0.034

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.355 ± 0.015

2.936 ± 0.02

4.989 ± 0.024

3.719 ± 0.021

6.825 ± 0.031

5.891 ± 0.027

5.517 ± 0.031

7.672 ± 0.025

1.373 ± 0.011

2.483 ± 0.017
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski