Paenibacillus phage Ash

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; Halcyonevirus; unclassified Halcyonevirus

Average proteome isoelectric point is 6.27

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 88 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A345ASU9|A0A345ASU9_9CAUD Uncharacterized protein OS=Paenibacillus phage Ash OX=2249763 GN=ASH_55 PE=4 SV=1
MM1 pKa = 7.88PEE3 pKa = 4.73LDD5 pKa = 3.53RR6 pKa = 11.84FDD8 pKa = 4.75YY9 pKa = 11.06DD10 pKa = 3.31GSEE13 pKa = 4.2PDD15 pKa = 3.8VVATCASCDD24 pKa = 3.6GEE26 pKa = 4.75VYY28 pKa = 10.71EE29 pKa = 5.47GDD31 pKa = 3.86DD32 pKa = 3.77VVLTTEE38 pKa = 4.04GDD40 pKa = 3.6IVHH43 pKa = 6.53EE44 pKa = 4.26EE45 pKa = 4.11CFAAFARR52 pKa = 11.84EE53 pKa = 3.83IYY55 pKa = 10.28RR56 pKa = 11.84NVSGTIDD63 pKa = 3.32ANGRR67 pKa = 11.84III69 pKa = 4.67

Molecular weight: 7.56 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A345ASP7|A0A345ASP7_9CAUD Terminase large subunit OS=Paenibacillus phage Ash OX=2249763 GN=ASH_3 PE=4 SV=1
MM1 pKa = 7.54SKK3 pKa = 11.05LEE5 pKa = 3.91VAASVLIGIFIIAFAAVLIFAIRR28 pKa = 11.84TAGLSPADD36 pKa = 3.68TALLITVYY44 pKa = 11.21ALTILSAIRR53 pKa = 11.84GLRR56 pKa = 11.84WLRR59 pKa = 3.61

Molecular weight: 6.29 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

88

0

88

17233

37

991

195.8

22.25

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.985 ± 0.248

0.992 ± 0.114

6.737 ± 0.2

8.188 ± 0.346

3.592 ± 0.169

6.696 ± 0.316

1.706 ± 0.114

6.435 ± 0.172

7.207 ± 0.392

8.211 ± 0.21

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.466 ± 0.121

3.859 ± 0.196

3.557 ± 0.188

3.041 ± 0.179

6.499 ± 0.303

5.13 ± 0.187

5.397 ± 0.186

6.58 ± 0.261

1.393 ± 0.127

4.329 ± 0.21
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski