Amino acid dipepetide frequency for Paenibacillus phage Ash

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
7.486AlaAla: 7.486 ± 0.81
1.045AlaCys: 1.045 ± 0.236
5.513AlaAsp: 5.513 ± 0.567
6.674AlaGlu: 6.674 ± 0.687
2.669AlaPhe: 2.669 ± 0.337
4.933AlaGly: 4.933 ± 0.808
0.929AlaHis: 0.929 ± 0.214
4.41AlaIle: 4.41 ± 0.485
6.093AlaLys: 6.093 ± 0.602
7.544AlaLeu: 7.544 ± 0.6
1.857AlaMet: 1.857 ± 0.291
3.772AlaAsn: 3.772 ± 0.475
2.263AlaPro: 2.263 ± 0.344
2.379AlaGln: 2.379 ± 0.379
3.946AlaArg: 3.946 ± 0.511
4.062AlaSer: 4.062 ± 0.509
3.482AlaThr: 3.482 ± 0.562
5.861AlaVal: 5.861 ± 0.631
1.567AlaTrp: 1.567 ± 0.369
3.366AlaTyr: 3.366 ± 0.496
0.0AlaXaa: 0.0 ± 0.0
Cys
0.87CysAla: 0.87 ± 0.255
0.058CysCys: 0.058 ± 0.064
0.58CysAsp: 0.58 ± 0.2
0.522CysGlu: 0.522 ± 0.191
0.464CysPhe: 0.464 ± 0.167
0.929CysGly: 0.929 ± 0.253
0.174CysHis: 0.174 ± 0.095
0.406CysIle: 0.406 ± 0.141
0.696CysLys: 0.696 ± 0.179
0.987CysLeu: 0.987 ± 0.241
0.058CysMet: 0.058 ± 0.056
0.29CysAsn: 0.29 ± 0.13
0.696CysPro: 0.696 ± 0.203
0.116CysGln: 0.116 ± 0.085
0.754CysArg: 0.754 ± 0.232
0.696CysSer: 0.696 ± 0.186
0.348CysThr: 0.348 ± 0.144
0.522CysVal: 0.522 ± 0.153
0.29CysTrp: 0.29 ± 0.125
0.464CysTyr: 0.464 ± 0.187
0.0CysXaa: 0.0 ± 0.0
Asp
4.643AspAla: 4.643 ± 0.556
0.406AspCys: 0.406 ± 0.155
5.513AspAsp: 5.513 ± 0.662
5.861AspGlu: 5.861 ± 0.533
3.25AspPhe: 3.25 ± 0.352
5.223AspGly: 5.223 ± 0.673
1.103AspHis: 1.103 ± 0.251
5.397AspIle: 5.397 ± 0.523
2.669AspLys: 2.669 ± 0.516
5.397AspLeu: 5.397 ± 0.578
1.625AspMet: 1.625 ± 0.327
2.611AspAsn: 2.611 ± 0.414
2.379AspPro: 2.379 ± 0.288
1.683AspGln: 1.683 ± 0.285
3.656AspArg: 3.656 ± 0.484
3.714AspSer: 3.714 ± 0.464
2.902AspThr: 2.902 ± 0.361
5.281AspVal: 5.281 ± 0.559
1.393AspTrp: 1.393 ± 0.306
2.669AspTyr: 2.669 ± 0.359
0.0AspXaa: 0.0 ± 0.0
Glu
6.093GluAla: 6.093 ± 0.605
0.87GluCys: 0.87 ± 0.291
4.526GluAsp: 4.526 ± 0.497
6.616GluGlu: 6.616 ± 0.619
2.611GluPhe: 2.611 ± 0.39
4.294GluGly: 4.294 ± 0.634
1.451GluHis: 1.451 ± 0.319
5.571GluIle: 5.571 ± 0.591
5.977GluLys: 5.977 ± 0.665
8.879GluLeu: 8.879 ± 0.758
2.263GluMet: 2.263 ± 0.348
2.844GluAsn: 2.844 ± 0.457
2.611GluPro: 2.611 ± 0.535
3.076GluGln: 3.076 ± 0.462
5.165GluArg: 5.165 ± 0.573
3.772GluSer: 3.772 ± 0.512
4.352GluThr: 4.352 ± 0.351
5.513GluVal: 5.513 ± 0.64
0.929GluTrp: 0.929 ± 0.244
4.468GluTyr: 4.468 ± 0.53
0.0GluXaa: 0.0 ± 0.0
Phe
3.018PheAla: 3.018 ± 0.395
0.116PheCys: 0.116 ± 0.077
2.786PheAsp: 2.786 ± 0.34
2.844PheGlu: 2.844 ± 0.46
1.509PhePhe: 1.509 ± 0.341
2.669PheGly: 2.669 ± 0.361
0.754PheHis: 0.754 ± 0.229
1.973PheIle: 1.973 ± 0.344
2.495PheLys: 2.495 ± 0.357
2.147PheLeu: 2.147 ± 0.34
1.045PheMet: 1.045 ± 0.27
1.915PheAsn: 1.915 ± 0.364
1.161PhePro: 1.161 ± 0.285
0.696PheGln: 0.696 ± 0.175
2.089PheArg: 2.089 ± 0.317
1.509PheSer: 1.509 ± 0.345
2.844PheThr: 2.844 ± 0.319
2.263PheVal: 2.263 ± 0.272
0.348PheTrp: 0.348 ± 0.13
1.741PheTyr: 1.741 ± 0.311
0.0PheXaa: 0.0 ± 0.0
Gly
3.598GlyAla: 3.598 ± 0.591
0.812GlyCys: 0.812 ± 0.24
4.701GlyAsp: 4.701 ± 0.561
5.745GlyGlu: 5.745 ± 0.758
2.321GlyPhe: 2.321 ± 0.397
4.817GlyGly: 4.817 ± 0.648
0.929GlyHis: 0.929 ± 0.25
5.745GlyIle: 5.745 ± 0.644
5.687GlyLys: 5.687 ± 0.569
5.513GlyLeu: 5.513 ± 0.595
1.857GlyMet: 1.857 ± 0.352
2.437GlyAsn: 2.437 ± 0.365
1.741GlyPro: 1.741 ± 0.281
1.799GlyGln: 1.799 ± 0.359
4.062GlyArg: 4.062 ± 0.486
2.669GlySer: 2.669 ± 0.501
3.946GlyThr: 3.946 ± 0.441
4.701GlyVal: 4.701 ± 0.513
1.161GlyTrp: 1.161 ± 0.219
2.727GlyTyr: 2.727 ± 0.375
0.0GlyXaa: 0.0 ± 0.0
His
1.451HisAla: 1.451 ± 0.269
0.232HisCys: 0.232 ± 0.119
1.219HisAsp: 1.219 ± 0.265
1.393HisGlu: 1.393 ± 0.287
0.522HisPhe: 0.522 ± 0.173
1.393HisGly: 1.393 ± 0.27
0.348HisHis: 0.348 ± 0.153
1.161HisIle: 1.161 ± 0.253
1.045HisLys: 1.045 ± 0.286
1.045HisLeu: 1.045 ± 0.288
0.29HisMet: 0.29 ± 0.121
0.464HisAsn: 0.464 ± 0.155
0.987HisPro: 0.987 ± 0.225
0.58HisGln: 0.58 ± 0.163
0.929HisArg: 0.929 ± 0.243
0.87HisSer: 0.87 ± 0.259
1.045HisThr: 1.045 ± 0.279
0.987HisVal: 0.987 ± 0.203
0.232HisTrp: 0.232 ± 0.135
0.87HisTyr: 0.87 ± 0.242
0.0HisXaa: 0.0 ± 0.0
Ile
5.455IleAla: 5.455 ± 0.639
0.638IleCys: 0.638 ± 0.217
5.919IleAsp: 5.919 ± 0.618
4.933IleGlu: 4.933 ± 0.449
1.915IlePhe: 1.915 ± 0.393
4.236IleGly: 4.236 ± 0.673
0.987IleHis: 0.987 ± 0.261
3.482IleIle: 3.482 ± 0.406
5.165IleLys: 5.165 ± 0.505
4.178IleLeu: 4.178 ± 0.483
1.277IleMet: 1.277 ± 0.313
2.437IleAsn: 2.437 ± 0.393
3.192IlePro: 3.192 ± 0.48
2.089IleGln: 2.089 ± 0.402
4.875IleArg: 4.875 ± 0.523
3.018IleSer: 3.018 ± 0.509
3.134IleThr: 3.134 ± 0.367
3.888IleVal: 3.888 ± 0.516
0.696IleTrp: 0.696 ± 0.204
2.844IleTyr: 2.844 ± 0.466
0.0IleXaa: 0.0 ± 0.0
Lys
5.165LysAla: 5.165 ± 0.562
0.638LysCys: 0.638 ± 0.221
4.12LysAsp: 4.12 ± 0.448
5.687LysGlu: 5.687 ± 0.773
2.263LysPhe: 2.263 ± 0.37
4.526LysGly: 4.526 ± 0.584
1.277LysHis: 1.277 ± 0.295
5.223LysIle: 5.223 ± 0.591
6.035LysLys: 6.035 ± 0.807
6.209LysLeu: 6.209 ± 0.638
2.031LysMet: 2.031 ± 0.437
2.031LysAsn: 2.031 ± 0.4
2.205LysPro: 2.205 ± 0.417
2.379LysGln: 2.379 ± 0.419
5.687LysArg: 5.687 ± 0.526
3.656LysSer: 3.656 ± 0.508
4.526LysThr: 4.526 ± 0.591
4.294LysVal: 4.294 ± 0.578
1.219LysTrp: 1.219 ± 0.342
2.902LysTyr: 2.902 ± 0.452
0.0LysXaa: 0.0 ± 0.0
Leu
8.182LeuAla: 8.182 ± 0.816
0.987LeuCys: 0.987 ± 0.254
6.151LeuAsp: 6.151 ± 0.518
6.674LeuGlu: 6.674 ± 0.808
2.844LeuPhe: 2.844 ± 0.417
4.12LeuGly: 4.12 ± 0.482
1.509LeuHis: 1.509 ± 0.317
4.991LeuIle: 4.991 ± 0.491
4.933LeuLys: 4.933 ± 0.641
5.745LeuLeu: 5.745 ± 0.644
1.857LeuMet: 1.857 ± 0.309
2.495LeuAsn: 2.495 ± 0.414
3.482LeuPro: 3.482 ± 0.395
2.669LeuGln: 2.669 ± 0.407
6.209LeuArg: 6.209 ± 0.7
5.803LeuSer: 5.803 ± 0.473
5.339LeuThr: 5.339 ± 0.535
4.294LeuVal: 4.294 ± 0.491
0.638LeuTrp: 0.638 ± 0.263
3.192LeuTyr: 3.192 ± 0.544
0.0LeuXaa: 0.0 ± 0.0
Met
2.147MetAla: 2.147 ± 0.354
0.058MetCys: 0.058 ± 0.06
1.103MetAsp: 1.103 ± 0.223
1.509MetGlu: 1.509 ± 0.278
0.987MetPhe: 0.987 ± 0.194
1.625MetGly: 1.625 ± 0.354
0.522MetHis: 0.522 ± 0.145
1.509MetIle: 1.509 ± 0.244
1.625MetLys: 1.625 ± 0.303
1.799MetLeu: 1.799 ± 0.279
0.754MetMet: 0.754 ± 0.236
1.393MetAsn: 1.393 ± 0.285
0.987MetPro: 0.987 ± 0.235
0.696MetGln: 0.696 ± 0.21
2.089MetArg: 2.089 ± 0.396
1.799MetSer: 1.799 ± 0.291
2.031MetThr: 2.031 ± 0.311
1.045MetVal: 1.045 ± 0.205
0.174MetTrp: 0.174 ± 0.11
0.812MetTyr: 0.812 ± 0.211
0.0MetXaa: 0.0 ± 0.0
Asn
2.786AsnAla: 2.786 ± 0.441
0.696AsnCys: 0.696 ± 0.21
2.321AsnAsp: 2.321 ± 0.372
3.598AsnGlu: 3.598 ± 0.407
1.509AsnPhe: 1.509 ± 0.283
4.352AsnGly: 4.352 ± 0.584
0.522AsnHis: 0.522 ± 0.159
1.451AsnIle: 1.451 ± 0.333
1.915AsnLys: 1.915 ± 0.375
2.379AsnLeu: 2.379 ± 0.372
0.929AsnMet: 0.929 ± 0.213
1.509AsnAsn: 1.509 ± 0.28
2.263AsnPro: 2.263 ± 0.459
1.103AsnGln: 1.103 ± 0.273
2.786AsnArg: 2.786 ± 0.529
2.147AsnSer: 2.147 ± 0.393
1.683AsnThr: 1.683 ± 0.326
2.495AsnVal: 2.495 ± 0.308
0.696AsnTrp: 0.696 ± 0.202
1.451AsnTyr: 1.451 ± 0.353
0.0AsnXaa: 0.0 ± 0.0
Pro
2.495ProAla: 2.495 ± 0.456
0.174ProCys: 0.174 ± 0.105
3.134ProAsp: 3.134 ± 0.453
3.25ProGlu: 3.25 ± 0.426
1.161ProPhe: 1.161 ± 0.266
2.321ProGly: 2.321 ± 0.425
0.638ProHis: 0.638 ± 0.161
2.263ProIle: 2.263 ± 0.285
3.076ProLys: 3.076 ± 0.395
2.263ProLeu: 2.263 ± 0.378
1.161ProMet: 1.161 ± 0.302
2.031ProAsn: 2.031 ± 0.326
0.929ProPro: 0.929 ± 0.258
0.812ProGln: 0.812 ± 0.206
1.973ProArg: 1.973 ± 0.46
1.393ProSer: 1.393 ± 0.239
2.437ProThr: 2.437 ± 0.479
1.799ProVal: 1.799 ± 0.279
0.464ProTrp: 0.464 ± 0.149
1.799ProTyr: 1.799 ± 0.326
0.0ProXaa: 0.0 ± 0.0
Gln
2.96GlnAla: 2.96 ± 0.461
0.174GlnCys: 0.174 ± 0.137
1.161GlnAsp: 1.161 ± 0.368
2.321GlnGlu: 2.321 ± 0.359
1.045GlnPhe: 1.045 ± 0.267
1.335GlnGly: 1.335 ± 0.296
0.522GlnHis: 0.522 ± 0.149
1.857GlnIle: 1.857 ± 0.311
2.263GlnLys: 2.263 ± 0.295
2.902GlnLeu: 2.902 ± 0.345
0.812GlnMet: 0.812 ± 0.273
1.335GlnAsn: 1.335 ± 0.318
0.464GlnPro: 0.464 ± 0.158
1.103GlnGln: 1.103 ± 0.327
2.495GlnArg: 2.495 ± 0.417
1.277GlnSer: 1.277 ± 0.259
2.263GlnThr: 2.263 ± 0.437
1.973GlnVal: 1.973 ± 0.336
0.696GlnTrp: 0.696 ± 0.2
1.393GlnTyr: 1.393 ± 0.246
0.0GlnXaa: 0.0 ± 0.0
Arg
4.643ArgAla: 4.643 ± 0.608
0.58ArgCys: 0.58 ± 0.17
3.656ArgAsp: 3.656 ± 0.472
6.732ArgGlu: 6.732 ± 0.801
2.031ArgPhe: 2.031 ± 0.418
3.25ArgGly: 3.25 ± 0.444
1.161ArgHis: 1.161 ± 0.315
4.352ArgIle: 4.352 ± 0.52
5.455ArgLys: 5.455 ± 0.725
6.674ArgLeu: 6.674 ± 0.637
2.205ArgMet: 2.205 ± 0.373
3.308ArgAsn: 3.308 ± 0.53
1.857ArgPro: 1.857 ± 0.321
2.205ArgGln: 2.205 ± 0.329
4.468ArgArg: 4.468 ± 0.749
2.669ArgSer: 2.669 ± 0.477
2.553ArgThr: 2.553 ± 0.35
3.714ArgVal: 3.714 ± 0.64
1.045ArgTrp: 1.045 ± 0.263
2.437ArgTyr: 2.437 ± 0.359
0.0ArgXaa: 0.0 ± 0.0
Ser
3.946SerAla: 3.946 ± 0.405
0.638SerCys: 0.638 ± 0.247
2.611SerAsp: 2.611 ± 0.413
4.236SerGlu: 4.236 ± 0.485
2.147SerPhe: 2.147 ± 0.328
4.004SerGly: 4.004 ± 0.461
0.812SerHis: 0.812 ± 0.237
3.018SerIle: 3.018 ± 0.35
4.178SerLys: 4.178 ± 0.493
3.482SerLeu: 3.482 ± 0.533
1.103SerMet: 1.103 ± 0.24
2.031SerAsn: 2.031 ± 0.38
2.321SerPro: 2.321 ± 0.463
1.393SerGln: 1.393 ± 0.309
2.844SerArg: 2.844 ± 0.442
3.308SerSer: 3.308 ± 0.439
2.205SerThr: 2.205 ± 0.401
3.83SerVal: 3.83 ± 0.579
0.638SerTrp: 0.638 ± 0.171
2.553SerTyr: 2.553 ± 0.368
0.0SerXaa: 0.0 ± 0.0
Thr
4.643ThrAla: 4.643 ± 0.584
0.29ThrCys: 0.29 ± 0.128
3.366ThrAsp: 3.366 ± 0.477
3.946ThrGlu: 3.946 ± 0.427
2.553ThrPhe: 2.553 ± 0.385
4.817ThrGly: 4.817 ± 0.478
0.929ThrHis: 0.929 ± 0.19
3.076ThrIle: 3.076 ± 0.421
4.004ThrLys: 4.004 ± 0.574
4.178ThrLeu: 4.178 ± 0.418
0.87ThrMet: 0.87 ± 0.249
1.509ThrAsn: 1.509 ± 0.302
2.495ThrPro: 2.495 ± 0.335
2.089ThrGln: 2.089 ± 0.373
2.553ThrArg: 2.553 ± 0.402
2.611ThrSer: 2.611 ± 0.368
2.263ThrThr: 2.263 ± 0.422
4.41ThrVal: 4.41 ± 0.574
0.58ThrTrp: 0.58 ± 0.182
2.786ThrTyr: 2.786 ± 0.372
0.0ThrXaa: 0.0 ± 0.0
Val
5.687ValAla: 5.687 ± 0.573
0.987ValCys: 0.987 ± 0.306
4.701ValAsp: 4.701 ± 0.498
4.584ValGlu: 4.584 ± 0.525
2.089ValPhe: 2.089 ± 0.36
3.83ValGly: 3.83 ± 0.528
1.335ValHis: 1.335 ± 0.282
4.584ValIle: 4.584 ± 0.577
4.875ValLys: 4.875 ± 0.623
6.035ValLeu: 6.035 ± 0.46
1.625ValMet: 1.625 ± 0.261
2.727ValAsn: 2.727 ± 0.462
2.031ValPro: 2.031 ± 0.322
1.625ValGln: 1.625 ± 0.288
4.236ValArg: 4.236 ± 0.394
3.366ValSer: 3.366 ± 0.455
3.772ValThr: 3.772 ± 0.481
4.468ValVal: 4.468 ± 0.641
0.58ValTrp: 0.58 ± 0.167
2.669ValTyr: 2.669 ± 0.469
0.0ValXaa: 0.0 ± 0.0
Trp
0.812TrpAla: 0.812 ± 0.222
0.0TrpCys: 0.0 ± 0.0
0.929TrpAsp: 0.929 ± 0.289
1.335TrpGlu: 1.335 ± 0.329
0.522TrpPhe: 0.522 ± 0.16
0.58TrpGly: 0.58 ± 0.174
0.464TrpHis: 0.464 ± 0.183
0.812TrpIle: 0.812 ± 0.215
0.987TrpLys: 0.987 ± 0.245
1.451TrpLeu: 1.451 ± 0.283
0.174TrpMet: 0.174 ± 0.097
0.464TrpAsn: 0.464 ± 0.156
0.232TrpPro: 0.232 ± 0.119
0.929TrpGln: 0.929 ± 0.242
0.754TrpArg: 0.754 ± 0.221
0.812TrpSer: 0.812 ± 0.227
0.929TrpThr: 0.929 ± 0.221
1.335TrpVal: 1.335 ± 0.32
0.058TrpTrp: 0.058 ± 0.061
0.348TrpTyr: 0.348 ± 0.149
0.0TrpXaa: 0.0 ± 0.0
Tyr
3.772TyrAla: 3.772 ± 0.444
0.522TyrCys: 0.522 ± 0.167
3.366TyrAsp: 3.366 ± 0.55
3.424TyrGlu: 3.424 ± 0.406
1.509TyrPhe: 1.509 ± 0.312
3.714TyrGly: 3.714 ± 0.482
0.696TyrHis: 0.696 ± 0.231
3.076TyrIle: 3.076 ± 0.45
2.902TyrLys: 2.902 ± 0.365
3.308TyrLeu: 3.308 ± 0.395
0.812TyrMet: 0.812 ± 0.232
0.987TyrAsn: 0.987 ± 0.241
1.277TyrPro: 1.277 ± 0.253
0.987TyrGln: 0.987 ± 0.245
3.424TyrArg: 3.424 ± 0.474
2.147TyrSer: 2.147 ± 0.378
1.915TyrThr: 1.915 ± 0.311
3.134TyrVal: 3.134 ± 0.427
0.522TyrTrp: 0.522 ± 0.18
1.799TyrTyr: 1.799 ± 0.369
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 88 proteins (17233 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski