Xylanibacterium ulmi
Average proteome isoelectric point is 6.32
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 3500 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A4Q7M6R4|A0A4Q7M6R4_9MICO Uncharacterized protein OS=Xylanibacterium ulmi OX=228973 GN=EV386_2664 PE=4 SV=1
MM1 pKa = 7.31 KK2 pKa = 10.12 MSMTKK7 pKa = 10.14 RR8 pKa = 11.84 AARR11 pKa = 11.84 VAALATAGALALAACTPGGDD31 pKa = 4.1 AADD34 pKa = 5.06 DD35 pKa = 4.21 GAATDD40 pKa = 3.7 AATDD44 pKa = 3.55 GGGAEE49 pKa = 4.17 GTVTVWHH56 pKa = 6.32 YY57 pKa = 10.99 FSDD60 pKa = 4.15 PQQVALMDD68 pKa = 4.28 KK69 pKa = 10.12 YY70 pKa = 11.59 AEE72 pKa = 4.24 LAEE75 pKa = 4.21 AANPGMTVDD84 pKa = 4.16 NVFVPYY90 pKa = 10.78 DD91 pKa = 3.39 QMNSQLISAAGAGQGPDD108 pKa = 3.14 VVIFNGAEE116 pKa = 3.79 TSTIALAGALAPLDD130 pKa = 4.49 DD131 pKa = 4.23 LWGTFADD138 pKa = 4.16 ADD140 pKa = 3.86 QFPDD144 pKa = 3.92 SVIHH148 pKa = 5.75 TVDD151 pKa = 2.76 GTMYY155 pKa = 10.9 AVQGYY160 pKa = 10.33 VNLLGLWYY168 pKa = 10.51 NQDD171 pKa = 3.18 ILDD174 pKa = 4.82 EE175 pKa = 5.24 IGVQPPTTIDD185 pKa = 3.49 EE186 pKa = 4.85 LEE188 pKa = 4.2 SAMAAAVAAGHH199 pKa = 6.47 GGITLSGLPNSQGEE213 pKa = 4.36 WQGFPWLSAEE223 pKa = 3.91 GFTYY227 pKa = 10.25 EE228 pKa = 4.53 NPSAQALEE236 pKa = 4.41 AGLTRR241 pKa = 11.84 VSNWVEE247 pKa = 4.16 QGWLPQEE254 pKa = 4.1 AVNWDD259 pKa = 3.29 QTVPFQQFAAGGFAFAANGNWQQGTAEE286 pKa = 4.25 ADD288 pKa = 2.97 ADD290 pKa = 3.72 FTYY293 pKa = 10.39 GVVPLPLGDD302 pKa = 3.72 SGQVYY307 pKa = 10.6 LGGEE311 pKa = 4.2 GAGIGANSANPEE323 pKa = 4.21 LAWAYY328 pKa = 7.53 LTSSYY333 pKa = 11.28 LSFEE337 pKa = 4.59 GNLAAADD344 pKa = 4.01 LVGSLPARR352 pKa = 11.84 ADD354 pKa = 3.39 AGQHH358 pKa = 6.63 DD359 pKa = 5.19 SVTSNEE365 pKa = 3.9 LLKK368 pKa = 10.87 PFAEE372 pKa = 4.85 TITMFGAQYY381 pKa = 10.09 PSAAIPAEE389 pKa = 3.93 AVADD393 pKa = 3.71 VQLRR397 pKa = 11.84 MGQAWSAVIGRR408 pKa = 11.84 QHH410 pKa = 6.38 SPADD414 pKa = 3.6 AAHH417 pKa = 6.23 TAMTALEE424 pKa = 4.26 GLLGG428 pKa = 3.85
Molecular weight: 44.2 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.706
IPC2_protein 3.745
IPC_protein 3.757
Toseland 3.541
ProMoST 3.923
Dawson 3.745
Bjellqvist 3.897
Wikipedia 3.668
Rodwell 3.592
Grimsley 3.452
Solomon 3.745
Lehninger 3.694
Nozaki 3.859
DTASelect 4.088
Thurlkill 3.592
EMBOSS 3.681
Sillero 3.884
Patrickios 0.922
IPC_peptide 3.745
IPC2_peptide 3.859
IPC2.peptide.svr19 3.776
Protein with the highest isoelectric point:
>tr|A0A4Q7M511|A0A4Q7M511_9MICO Phospholipase D-like protein OS=Xylanibacterium ulmi OX=228973 GN=EV386_3404 PE=4 SV=1
MM1 pKa = 7.4 GSVIKK6 pKa = 10.42 KK7 pKa = 8.47 RR8 pKa = 11.84 RR9 pKa = 11.84 KK10 pKa = 9.22 RR11 pKa = 11.84 MAKK14 pKa = 9.41 KK15 pKa = 9.87 KK16 pKa = 9.7 HH17 pKa = 5.84 RR18 pKa = 11.84 KK19 pKa = 8.56 LLRR22 pKa = 11.84 KK23 pKa = 7.78 TRR25 pKa = 11.84 HH26 pKa = 3.65 QRR28 pKa = 11.84 RR29 pKa = 11.84 NKK31 pKa = 9.78 KK32 pKa = 9.85
Molecular weight: 4.08 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.493
IPC2_protein 11.14
IPC_protein 12.691
Toseland 12.866
ProMoST 13.349
Dawson 12.866
Bjellqvist 12.852
Wikipedia 13.334
Rodwell 12.735
Grimsley 12.91
Solomon 13.349
Lehninger 13.261
Nozaki 12.866
DTASelect 12.852
Thurlkill 12.866
EMBOSS 13.364
Sillero 12.866
Patrickios 12.457
IPC_peptide 13.349
IPC2_peptide 12.34
IPC2.peptide.svr19 9.071
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
3500
0
3500
1185822
30
2393
338.8
35.88
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
15.151 ± 0.067
0.577 ± 0.01
6.268 ± 0.034
5.052 ± 0.036
2.618 ± 0.026
9.296 ± 0.035
2.113 ± 0.019
3.161 ± 0.031
1.538 ± 0.025
10.154 ± 0.043
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
1.609 ± 0.015
1.569 ± 0.022
5.956 ± 0.034
2.756 ± 0.021
7.903 ± 0.047
4.897 ± 0.027
6.184 ± 0.035
9.867 ± 0.04
1.536 ± 0.018
1.794 ± 0.02
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here