Streptococcus satellite phage Javan167
Average proteome isoelectric point is 7.06
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 12 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A4D5ZF38|A0A4D5ZF38_9VIRU Uncharacterized protein OS=Streptococcus satellite phage Javan167 OX=2558552 GN=JavanS167_0001 PE=4 SV=1
MM1 pKa = 7.49 KK2 pKa = 10.2 KK3 pKa = 9.75 IKK5 pKa = 10.71 LFVKK9 pKa = 9.42 EE10 pKa = 4.23 DD11 pKa = 3.32 RR12 pKa = 11.84 SIEE15 pKa = 3.94 EE16 pKa = 4.27 DD17 pKa = 3.61 VNTFIEE23 pKa = 4.68 DD24 pKa = 3.71 KK25 pKa = 10.87 NVVDD29 pKa = 5.49 IDD31 pKa = 4.02 FQVATSGSNSAVFVSIVYY49 pKa = 8.92 EE50 pKa = 4.14 VV51 pKa = 2.88
Molecular weight: 5.8 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 4.671
IPC2_protein 4.546
IPC_protein 4.342
Toseland 4.164
ProMoST 4.469
Dawson 4.304
Bjellqvist 4.457
Wikipedia 4.202
Rodwell 4.177
Grimsley 4.088
Solomon 4.291
Lehninger 4.24
Nozaki 4.418
DTASelect 4.584
Thurlkill 4.202
EMBOSS 4.215
Sillero 4.457
Patrickios 4.279
IPC_peptide 4.291
IPC2_peptide 4.431
IPC2.peptide.svr19 4.41
Protein with the highest isoelectric point:
>tr|A0A4D5ZFJ7|A0A4D5ZFJ7_9VIRU Uncharacterized protein OS=Streptococcus satellite phage Javan167 OX=2558552 GN=JavanS167_0009 PE=4 SV=1
MM1 pKa = 7.78 KK2 pKa = 10.12 INEE5 pKa = 4.02 ITKK8 pKa = 9.72 QDD10 pKa = 3.0 GTIIYY15 pKa = 9.72 RR16 pKa = 11.84 SSVYY20 pKa = 10.64 LGIDD24 pKa = 2.94 QVTGKK29 pKa = 10.58 KK30 pKa = 7.9 VTTKK34 pKa = 9.06 ITGRR38 pKa = 11.84 TRR40 pKa = 11.84 KK41 pKa = 8.73 EE42 pKa = 3.9 VKK44 pKa = 9.97 QKK46 pKa = 11.01 AKK48 pKa = 10.24 QEE50 pKa = 4.16 EE51 pKa = 4.35 IEE53 pKa = 4.67 FIQNGSTRR61 pKa = 11.84 FQATTIKK68 pKa = 10.61 NYY70 pKa = 10.64 EE71 pKa = 3.9 EE72 pKa = 5.24 LAWLWWDD79 pKa = 3.78 SYY81 pKa = 11.26 KK82 pKa = 9.88 HH83 pKa = 4.59 TVKK86 pKa = 10.7 PNTQDD91 pKa = 3.08 FTRR94 pKa = 11.84 KK95 pKa = 8.7 VLKK98 pKa = 10.51 NHH100 pKa = 6.48 IVPLFGAYY108 pKa = 9.55 KK109 pKa = 10.1 LDD111 pKa = 3.65 KK112 pKa = 10.12 LTTPLIQSTMNRR124 pKa = 11.84 LADD127 pKa = 3.57 KK128 pKa = 9.94 TNKK131 pKa = 9.63 GEE133 pKa = 3.95 KK134 pKa = 9.57 GAYY137 pKa = 8.69 LHH139 pKa = 6.48 YY140 pKa = 11.01 DD141 pKa = 3.92 KK142 pKa = 11.13 IHH144 pKa = 6.25 GLNKK148 pKa = 10.25 RR149 pKa = 11.84 ILQYY153 pKa = 10.83 GVVMQVIPYY162 pKa = 8.76 NPARR166 pKa = 11.84 EE167 pKa = 4.21 VILPRR172 pKa = 11.84 NTKK175 pKa = 9.97 KK176 pKa = 10.81 AEE178 pKa = 3.88 QKK180 pKa = 10.33 KK181 pKa = 10.04 IKK183 pKa = 10.68 HH184 pKa = 6.31 FDD186 pKa = 3.46 NQEE189 pKa = 3.48 LKK191 pKa = 10.5 MFLDD195 pKa = 4.1 YY196 pKa = 11.4 LEE198 pKa = 5.41 SLDD201 pKa = 3.81 KK202 pKa = 10.77 EE203 pKa = 4.37 RR204 pKa = 11.84 YY205 pKa = 8.41 RR206 pKa = 11.84 YY207 pKa = 10.0 LYY209 pKa = 10.49 EE210 pKa = 4.11 DD211 pKa = 3.16 TLYY214 pKa = 11.02 RR215 pKa = 11.84 FLLATGCRR223 pKa = 11.84 INEE226 pKa = 4.0 ALALSWSDD234 pKa = 2.88 IDD236 pKa = 4.45 LDD238 pKa = 3.81 NAVVHH243 pKa = 5.5 ITKK246 pKa = 9.24 TLNYY250 pKa = 8.53 KK251 pKa = 9.41 QEE253 pKa = 4.01 INTPKK258 pKa = 10.43 SKK260 pKa = 10.64 ASHH263 pKa = 6.73 RR264 pKa = 11.84 DD265 pKa = 2.71 IDD267 pKa = 3.63 IDD269 pKa = 3.57 QATISMLKK277 pKa = 10.08 AYY279 pKa = 7.65 QLRR282 pKa = 11.84 QIQEE286 pKa = 3.53 GWKK289 pKa = 10.12 LGRR292 pKa = 11.84 TEE294 pKa = 4.38 SVVFSDD300 pKa = 6.25 FIHH303 pKa = 7.63 DD304 pKa = 3.9 YY305 pKa = 10.21 PSNRR309 pKa = 11.84 TLQTRR314 pKa = 11.84 LRR316 pKa = 11.84 THH318 pKa = 6.83 FKK320 pKa = 10.42 RR321 pKa = 11.84 AGVTNIGFHH330 pKa = 6.07 GFRR333 pKa = 11.84 HH334 pKa = 4.87 THH336 pKa = 6.58 ASLLLNSGIPYY347 pKa = 10.0 KK348 pKa = 10.34 EE349 pKa = 3.8 LQHH352 pKa = 6.73 RR353 pKa = 11.84 LGHH356 pKa = 5.68 STLSMTMDD364 pKa = 4.17 TYY366 pKa = 11.8 SHH368 pKa = 7.15 LSKK371 pKa = 10.96 EE372 pKa = 4.3 NAKK375 pKa = 10.37 KK376 pKa = 10.42 AVSFYY381 pKa = 11.09 EE382 pKa = 4.26 KK383 pKa = 10.47 ALQSII388 pKa = 4.63
Molecular weight: 45.24 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.098
IPC2_protein 9.136
IPC_protein 9.019
Toseland 9.78
ProMoST 9.487
Dawson 10.014
Bjellqvist 9.663
Wikipedia 10.175
Rodwell 10.438
Grimsley 10.087
Solomon 10.028
Lehninger 9.984
Nozaki 9.765
DTASelect 9.663
Thurlkill 9.853
EMBOSS 10.189
Sillero 9.911
Patrickios 7.614
IPC_peptide 10.028
IPC2_peptide 8.126
IPC2.peptide.svr19 8.217
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
12
0
12
2257
51
388
188.1
21.8
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
4.918 ± 0.588
0.443 ± 0.135
5.45 ± 0.386
8.595 ± 0.635
4.342 ± 0.273
4.519 ± 0.589
1.772 ± 0.437
7.089 ± 0.563
10.722 ± 0.322
9.88 ± 0.686
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.127 ± 0.282
6.203 ± 0.364
2.525 ± 0.308
4.298 ± 0.324
4.165 ± 0.459
6.159 ± 0.612
6.557 ± 0.643
4.829 ± 0.463
0.798 ± 0.118
4.608 ± 0.567
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here