Tortoise microvirus 106
Average proteome isoelectric point is 7.38
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 5 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A4P8W705|A0A4P8W705_9VIRU DNA pilot protein OS=Tortoise microvirus 106 OX=2583106 PE=4 SV=1
MM1 pKa = 7.55 NSNKK5 pKa = 9.44 TKK7 pKa = 10.99 GNIFQNVLSADD18 pKa = 3.81 VPQSQFDD25 pKa = 3.66 LSFEE29 pKa = 4.24 NKK31 pKa = 8.27 LTCDD35 pKa = 3.43 MGEE38 pKa = 5.22 LIPICCQEE46 pKa = 4.5 ILPGDD51 pKa = 4.08 KK52 pKa = 10.17 FQCDD56 pKa = 3.31 ANVFIRR62 pKa = 11.84 FQPLIAPIMHH72 pKa = 7.01 NIDD75 pKa = 3.06 VHH77 pKa = 5.64 VRR79 pKa = 11.84 WFFVPNRR86 pKa = 11.84 LVWNEE91 pKa = 3.75 FEE93 pKa = 5.42 DD94 pKa = 5.42 FITGGTTGMEE104 pKa = 4.21 SPIYY108 pKa = 9.66 PYY110 pKa = 10.46 RR111 pKa = 11.84 EE112 pKa = 4.06 YY113 pKa = 11.06 KK114 pKa = 10.28 EE115 pKa = 4.17 SARR118 pKa = 11.84 LGSLEE123 pKa = 5.27 DD124 pKa = 3.73 YY125 pKa = 10.88 LGVPTEE131 pKa = 4.25 TPEE134 pKa = 4.73 GLFGMHH140 pKa = 6.89 NIIVNMLPFRR150 pKa = 11.84 GYY152 pKa = 10.25 ALIWNEE158 pKa = 3.64 YY159 pKa = 10.36 FRR161 pKa = 11.84 DD162 pKa = 3.68 QTLDD166 pKa = 3.12 TEE168 pKa = 4.44 MEE170 pKa = 3.98 IARR173 pKa = 11.84 GSGEE177 pKa = 3.87 DD178 pKa = 3.17 TKK180 pKa = 11.32 DD181 pKa = 3.18 YY182 pKa = 10.74 FLRR185 pKa = 11.84 RR186 pKa = 11.84 VRR188 pKa = 11.84 WEE190 pKa = 3.41 KK191 pKa = 11.2 DD192 pKa = 3.24 YY193 pKa = 9.28 FTSALPWTQRR203 pKa = 11.84 GEE205 pKa = 4.2 PVHH208 pKa = 6.08 MPPMTLRR215 pKa = 11.84 EE216 pKa = 4.14 GLHH219 pKa = 6.85 PDD221 pKa = 3.32 MVIQDD226 pKa = 4.27 TIRR229 pKa = 11.84 IVDD232 pKa = 3.47 ASGNTFSYY240 pKa = 10.65 PEE242 pKa = 3.94 NPLYY246 pKa = 10.83 GNPYY250 pKa = 10.17 RR251 pKa = 11.84 LGHH254 pKa = 5.45 QSGVVTDD261 pKa = 4.65 PNEE264 pKa = 3.84 KK265 pKa = 10.33 PIFFDD270 pKa = 4.71 FSAYY274 pKa = 10.31 DD275 pKa = 3.37 SYY277 pKa = 11.6 TINQLRR283 pKa = 11.84 EE284 pKa = 3.84 ANAVQRR290 pKa = 11.84 FLEE293 pKa = 4.33 RR294 pKa = 11.84 QAVGGARR301 pKa = 11.84 YY302 pKa = 10.24 AEE304 pKa = 3.99 TLLNHH309 pKa = 6.81 FGVRR313 pKa = 11.84 TADD316 pKa = 3.31 SRR318 pKa = 11.84 LQRR321 pKa = 11.84 PEE323 pKa = 3.5 YY324 pKa = 10.45 LGGHH328 pKa = 5.73 RR329 pKa = 11.84 QPVTVSEE336 pKa = 4.37 VLQQSEE342 pKa = 4.63 SSATSPQGNMAGRR355 pKa = 11.84 AISTGSFGSEE365 pKa = 3.38 STLFTEE371 pKa = 4.56 HH372 pKa = 7.02 GYY374 pKa = 11.06 LFGLMYY380 pKa = 10.47 VRR382 pKa = 11.84 PQTGYY387 pKa = 8.53 TNTFKK392 pKa = 11.23 KK393 pKa = 9.75 MLHH396 pKa = 5.27 QKK398 pKa = 10.28 ADD400 pKa = 3.34 KK401 pKa = 10.35 FDD403 pKa = 4.04 YY404 pKa = 10.38 AWPEE408 pKa = 3.78 FASLGEE414 pKa = 3.93 QEE416 pKa = 4.49 ILQKK420 pKa = 10.13 EE421 pKa = 4.48 VHH423 pKa = 5.86 VGTRR427 pKa = 11.84 TGLEE431 pKa = 4.07 SNNVFGYY438 pKa = 7.5 QSRR441 pKa = 11.84 YY442 pKa = 10.44 AEE444 pKa = 3.83 YY445 pKa = 10.54 KK446 pKa = 9.1 FNNNEE451 pKa = 3.63 VHH453 pKa = 7.21 GSFKK457 pKa = 10.61 KK458 pKa = 10.55 DD459 pKa = 3.03 LSFWHH464 pKa = 7.13 LARR467 pKa = 11.84 LYY469 pKa = 10.86 FRR471 pKa = 11.84 DD472 pKa = 3.81 YY473 pKa = 11.55 DD474 pKa = 3.87 PALDD478 pKa = 3.66 ATFVACEE485 pKa = 3.74 PRR487 pKa = 11.84 KK488 pKa = 10.6 DD489 pKa = 3.1 IFAVVSEE496 pKa = 4.63 DD497 pKa = 3.94 RR498 pKa = 11.84 NDD500 pKa = 3.52 SLLVEE505 pKa = 4.72 VYY507 pKa = 11.04 NKK509 pKa = 8.52 ITAIRR514 pKa = 11.84 PLPAFGKK521 pKa = 9.92 PSLL524 pKa = 4.01
Molecular weight: 60.02 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 5.174
IPC2_protein 5.232
IPC_protein 5.207
Toseland 5.27
ProMoST 5.397
Dawson 5.258
Bjellqvist 5.359
Wikipedia 5.156
Rodwell 5.194
Grimsley 5.245
Solomon 5.258
Lehninger 5.219
Nozaki 5.41
DTASelect 5.563
Thurlkill 5.308
EMBOSS 5.258
Sillero 5.486
Patrickios 3.884
IPC_peptide 5.27
IPC2_peptide 5.499
IPC2.peptide.svr19 5.408
Protein with the highest isoelectric point:
>tr|A0A4P8WAH3|A0A4P8WAH3_9VIRU Uncharacterized protein OS=Tortoise microvirus 106 OX=2583106 PE=4 SV=1
MM1 pKa = 7.53 LNIEE5 pKa = 5.16 KK6 pKa = 10.67 SLTTNNQYY14 pKa = 10.62 IIFVHH19 pKa = 6.17 SYY21 pKa = 9.66 KK22 pKa = 10.68 NYY24 pKa = 10.3 TIMEE28 pKa = 4.31 TLKK31 pKa = 11.04 NLLNRR36 pKa = 11.84 NDD38 pKa = 3.55 VVRR41 pKa = 11.84 LMKK44 pKa = 10.87 NLDD47 pKa = 3.73 GLSLPFLFFVKK58 pKa = 10.47 PSDD61 pKa = 3.67 RR62 pKa = 11.84 SSRR65 pKa = 11.84 WLLKK69 pKa = 9.39 ASKK72 pKa = 10.27 RR73 pKa = 11.84 IGAFGSIKK81 pKa = 10.29 DD82 pKa = 3.58 ISEE85 pKa = 4.72 SIPILDD91 pKa = 4.64 LFTGKK96 pKa = 9.73 IMAITVLVRR105 pKa = 11.84 DD106 pKa = 3.47 EE107 pKa = 4.11 WFEE110 pKa = 4.26 LFVMDD115 pKa = 4.99 VPFSIKK121 pKa = 9.99 KK122 pKa = 9.8 EE123 pKa = 3.92 KK124 pKa = 10.74 LLLSLFLKK132 pKa = 10.6 EE133 pKa = 4.05 SGNRR137 pKa = 11.84 RR138 pKa = 11.84 SFSYY142 pKa = 11.11 NNYY145 pKa = 9.43 ILQLFNSLKK154 pKa = 10.71 NKK156 pKa = 10.29 SLL158 pKa = 3.56
Molecular weight: 18.45 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.002
IPC2_protein 9.224
IPC_protein 9.151
Toseland 10.058
ProMoST 9.633
Dawson 10.204
Bjellqvist 9.823
Wikipedia 10.335
Rodwell 10.789
Grimsley 10.262
Solomon 10.233
Lehninger 10.204
Nozaki 10.014
DTASelect 9.823
Thurlkill 10.072
EMBOSS 10.438
Sillero 10.116
Patrickios 10.496
IPC_peptide 10.233
IPC2_peptide 8.2
IPC2.peptide.svr19 8.254
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
5
0
5
1316
119
524
263.2
30.46
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
5.015 ± 0.774
1.14 ± 0.489
5.015 ± 0.401
6.687 ± 0.401
4.635 ± 1.209
5.167 ± 0.937
2.052 ± 0.554
5.243 ± 0.698
6.535 ± 1.327
9.574 ± 1.304
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.888 ± 0.227
6.687 ± 1.009
5.167 ± 0.722
4.635 ± 0.904
5.775 ± 0.232
7.447 ± 0.953
4.787 ± 0.921
4.787 ± 0.655
1.292 ± 0.186
5.471 ± 0.798
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here