Arthrobacter sp. 785
Average proteome isoelectric point is 6.09
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 3176 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A5N6MDX7|A0A5N6MDX7_9MICC SDR family oxidoreductase OS=Arthrobacter sp. 785 OX=2606616 GN=GD627_14915 PE=3 SV=1
MM1 pKa = 7.42 ARR3 pKa = 11.84 ALIIVDD9 pKa = 3.7 VQNDD13 pKa = 3.63 FCEE16 pKa = 4.88 GGSLAVAGGADD27 pKa = 3.88 LAGEE31 pKa = 3.89 ITDD34 pKa = 3.89 YY35 pKa = 11.64 VEE37 pKa = 4.31 TSAARR42 pKa = 11.84 YY43 pKa = 9.58 DD44 pKa = 4.0 LVAATQDD51 pKa = 3.07 WHH53 pKa = 7.33 IEE55 pKa = 4.0 PGAHH59 pKa = 6.85 FSEE62 pKa = 4.98 TPDD65 pKa = 4.43 FAQSWPPHH73 pKa = 5.83 CVAGTAGAQLHH84 pKa = 7.05 PDD86 pKa = 3.89 LDD88 pKa = 4.24 TEE90 pKa = 4.46 LVDD93 pKa = 3.66 AFFRR97 pKa = 11.84 KK98 pKa = 9.24 GQYY101 pKa = 8.18 EE102 pKa = 3.77 AAYY105 pKa = 10.34 SGFEE109 pKa = 4.14 GVLAPDD115 pKa = 3.54 VEE117 pKa = 4.75 VPLGEE122 pKa = 5.09 AEE124 pKa = 4.52 AEE126 pKa = 4.1 PDD128 pKa = 3.8 TEE130 pKa = 4.39 TLSLDD135 pKa = 3.11 DD136 pKa = 4.07 WLRR139 pKa = 11.84 EE140 pKa = 3.8 NDD142 pKa = 3.11 VDD144 pKa = 3.91 EE145 pKa = 4.56 VVVVGLAADD154 pKa = 3.63 YY155 pKa = 10.47 CVRR158 pKa = 11.84 ATALDD163 pKa = 3.82 AVAAGYY169 pKa = 6.01 TTAVIPEE176 pKa = 4.6 LCRR179 pKa = 11.84 GIKK182 pKa = 10.15 RR183 pKa = 11.84 EE184 pKa = 4.04 TTLAAWAEE192 pKa = 4.24 CEE194 pKa = 4.06 DD195 pKa = 4.19 AGVEE199 pKa = 4.93 IIDD202 pKa = 3.91 LL203 pKa = 4.12
Molecular weight: 21.6 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.707
IPC2_protein 3.808
IPC_protein 3.795
Toseland 3.592
ProMoST 3.948
Dawson 3.77
Bjellqvist 3.935
Wikipedia 3.694
Rodwell 3.63
Grimsley 3.503
Solomon 3.77
Lehninger 3.719
Nozaki 3.884
DTASelect 4.088
Thurlkill 3.63
EMBOSS 3.706
Sillero 3.91
Patrickios 1.125
IPC_peptide 3.77
IPC2_peptide 3.897
IPC2.peptide.svr19 3.823
Protein with the highest isoelectric point:
>tr|A0A5N6MSW2|A0A5N6MSW2_9MICC ATP-dependent RNA helicase DeaD OS=Arthrobacter sp. 785 OX=2606616 GN=deaD PE=3 SV=1
MM1 pKa = 7.4 GSVIKK6 pKa = 10.42 KK7 pKa = 8.47 RR8 pKa = 11.84 RR9 pKa = 11.84 KK10 pKa = 9.22 RR11 pKa = 11.84 MAKK14 pKa = 9.41 KK15 pKa = 9.87 KK16 pKa = 9.7 HH17 pKa = 5.84 RR18 pKa = 11.84 KK19 pKa = 8.56 LLRR22 pKa = 11.84 KK23 pKa = 7.78 TRR25 pKa = 11.84 HH26 pKa = 3.65 QRR28 pKa = 11.84 RR29 pKa = 11.84 NKK31 pKa = 9.78 KK32 pKa = 9.85
Molecular weight: 4.08 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.493
IPC2_protein 11.14
IPC_protein 12.691
Toseland 12.866
ProMoST 13.349
Dawson 12.866
Bjellqvist 12.852
Wikipedia 13.334
Rodwell 12.735
Grimsley 12.91
Solomon 13.349
Lehninger 13.261
Nozaki 12.866
DTASelect 12.852
Thurlkill 12.866
EMBOSS 13.364
Sillero 12.866
Patrickios 12.457
IPC_peptide 13.349
IPC2_peptide 12.34
IPC2.peptide.svr19 9.071
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
3176
0
3176
1047178
29
1875
329.7
35.23
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
13.197 ± 0.067
0.598 ± 0.011
5.686 ± 0.032
5.798 ± 0.043
3.164 ± 0.03
9.278 ± 0.042
1.877 ± 0.021
4.149 ± 0.034
2.406 ± 0.032
10.376 ± 0.046
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.023 ± 0.017
2.391 ± 0.026
5.57 ± 0.035
2.973 ± 0.023
6.847 ± 0.048
5.94 ± 0.032
5.865 ± 0.03
8.348 ± 0.031
1.378 ± 0.017
2.136 ± 0.022
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here