Microviridae sp. ctzVR26
Average proteome isoelectric point is 7.61
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 8 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A5Q2WAI5|A0A5Q2WAI5_9VIRU Uncharacterized protein OS=Microviridae sp. ctzVR26 OX=2656706 PE=4 SV=1
MM1 pKa = 7.68 AEE3 pKa = 4.81 KK4 pKa = 9.52 IDD6 pKa = 3.85 KK7 pKa = 9.0 KK8 pKa = 8.72 TGEE11 pKa = 4.45 VIEE14 pKa = 4.35 SGYY17 pKa = 10.88 VKK19 pKa = 10.37 PLVFDD24 pKa = 3.9 SVEE27 pKa = 4.0 SSLTKK32 pKa = 10.09 FDD34 pKa = 3.09 AHH36 pKa = 6.23 GRR38 pKa = 11.84 EE39 pKa = 4.23 IPDD42 pKa = 3.41 RR43 pKa = 11.84 RR44 pKa = 11.84 PLEE47 pKa = 4.01 LLISLQTAVKK57 pKa = 9.93 TNEE60 pKa = 3.83 QKK62 pKa = 10.1 MKK64 pKa = 10.59 EE65 pKa = 4.26 VIARR69 pKa = 11.84 EE70 pKa = 4.02 LNKK73 pKa = 10.16 LARR76 pKa = 11.84 ARR78 pKa = 11.84 GFEE81 pKa = 4.07 TLEE84 pKa = 3.95 EE85 pKa = 4.49 SNDD88 pKa = 3.24 FSMEE92 pKa = 3.87 TDD94 pKa = 3.76 DD95 pKa = 4.44 YY96 pKa = 11.54 AALSGYY102 pKa = 8.94 EE103 pKa = 3.91 FSEE106 pKa = 4.17 EE107 pKa = 3.78 LSYY110 pKa = 10.43 MDD112 pKa = 5.0 KK113 pKa = 11.21 APDD116 pKa = 3.41 AAAPAAAPAAAPAPVPAPEE135 pKa = 4.22 GAKK138 pKa = 10.33
Molecular weight: 15.03 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 4.791
IPC2_protein 4.724
IPC_protein 4.609
Toseland 4.457
ProMoST 4.685
Dawson 4.533
Bjellqvist 4.685
Wikipedia 4.38
Rodwell 4.444
Grimsley 4.368
Solomon 4.533
Lehninger 4.482
Nozaki 4.635
DTASelect 4.749
Thurlkill 4.444
EMBOSS 4.393
Sillero 4.711
Patrickios 4.228
IPC_peptide 4.533
IPC2_peptide 4.698
IPC2.peptide.svr19 4.678
Protein with the highest isoelectric point:
>tr|A0A5Q2WAI5|A0A5Q2WAI5_9VIRU Uncharacterized protein OS=Microviridae sp. ctzVR26 OX=2656706 PE=4 SV=1
MM1 pKa = 7.75 AKK3 pKa = 10.13 PKK5 pKa = 10.36 RR6 pKa = 11.84 KK7 pKa = 9.47 NLLVQNSYY15 pKa = 11.24 LEE17 pKa = 4.3 RR18 pKa = 11.84 KK19 pKa = 9.57 VLDD22 pKa = 3.64 FAKK25 pKa = 10.38 PRR27 pKa = 11.84 LLVPLTLTKK36 pKa = 10.3 TIADD40 pKa = 3.36 ARR42 pKa = 11.84 LYY44 pKa = 10.55 RR45 pKa = 11.84 PQPQIKK51 pKa = 9.4 RR52 pKa = 11.84 DD53 pKa = 3.54 NYY55 pKa = 9.95 LKK57 pKa = 10.83 KK58 pKa = 10.64 LVVLQSYY65 pKa = 9.88 LKK67 pKa = 9.38 SQKK70 pKa = 9.9 RR71 pKa = 11.84 PSGLTQGPFRR81 pKa = 11.84 AFNNPSLAIRR91 pKa = 11.84 MATCQSRR98 pKa = 11.84 HH99 pKa = 4.09 TRR101 pKa = 11.84 KK102 pKa = 9.59 EE103 pKa = 3.53 VLHH106 pKa = 5.66 ALKK109 pKa = 9.61 RR110 pKa = 11.84 TGKK113 pKa = 10.65 GSGSKK118 pKa = 9.74 PRR120 pKa = 11.84 RR121 pKa = 11.84 HH122 pKa = 5.41 SWKK125 pKa = 10.61 SAIKK129 pKa = 10.37 CC130 pKa = 3.67
Molecular weight: 14.96 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.212
IPC2_protein 10.262
IPC_protein 11.301
Toseland 11.506
ProMoST 11.769
Dawson 11.535
Bjellqvist 11.403
Wikipedia 11.886
Rodwell 11.711
Grimsley 11.564
Solomon 11.886
Lehninger 11.813
Nozaki 11.491
DTASelect 11.403
Thurlkill 11.491
EMBOSS 11.959
Sillero 11.506
Patrickios 11.418
IPC_peptide 11.886
IPC2_peptide 10.774
IPC2.peptide.svr19 8.703
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
8
0
8
1497
32
500
187.1
20.94
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
8.884 ± 1.001
0.534 ± 0.187
5.544 ± 0.64
5.544 ± 1.269
3.607 ± 0.535
7.214 ± 0.88
2.071 ± 0.583
4.208 ± 0.537
6.546 ± 1.329
9.018 ± 0.669
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
3.14 ± 0.484
4.409 ± 0.476
5.277 ± 0.763
3.874 ± 0.565
6.546 ± 0.873
6.413 ± 0.378
6.012 ± 0.854
6.079 ± 0.894
1.403 ± 0.395
3.674 ± 0.453
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here