Candidatus Electrothrix aarhusiensis
Average proteome isoelectric point is 6.62
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 4071 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A444ISR4|A0A444ISR4_9DELT PD-(D/E)XK nuclease superfamily protein OS=Candidatus Electrothrix aarhusiensis OX=1859131 GN=H206_02607 PE=4 SV=1
MM1 pKa = 8.19 PDD3 pKa = 2.94 RR4 pKa = 11.84 TFGDD8 pKa = 3.33 KK9 pKa = 10.87 GVLITDD15 pKa = 4.52 DD16 pKa = 5.3 DD17 pKa = 4.92 NDD19 pKa = 4.06 TVFYY23 pKa = 10.8 DD24 pKa = 3.54 VLMTEE29 pKa = 4.45 KK30 pKa = 9.83 MIAATGVTVGEE41 pKa = 4.2 EE42 pKa = 4.2 EE43 pKa = 4.0 MRR45 pKa = 11.84 EE46 pKa = 4.02 ALLITYY52 pKa = 8.9 AQGDD56 pKa = 4.12 GANSVQLFQQQVAAASEE73 pKa = 4.41 GAAEE77 pKa = 4.08 ATEE80 pKa = 4.38 AADD83 pKa = 3.79 EE84 pKa = 4.56 DD85 pKa = 4.37 SEE87 pKa = 4.35 EE88 pKa = 4.51 TEE90 pKa = 4.21 TVAQIVTTEE99 pKa = 3.57 VDD101 pKa = 3.23 NEE103 pKa = 3.51 EE104 pKa = 5.14 DD105 pKa = 4.0 YY106 pKa = 11.76 AFSLAAADD114 pKa = 3.83 AGSVVVVGASGAQEE128 pKa = 3.69 IASAAVRR135 pKa = 11.84 KK136 pKa = 7.8 YY137 pKa = 9.38 TIFQSSVTGSSWSTATGNASILTGDD162 pKa = 3.72 AQNVTRR168 pKa = 11.84 TTALIPGEE176 pKa = 4.18 VLSGLGTVTEE186 pKa = 4.25 RR187 pKa = 11.84 GVVFSTDD194 pKa = 3.58 PLPVLKK200 pKa = 10.78 GSDD203 pKa = 3.46 STTDD207 pKa = 3.29 DD208 pKa = 3.69 TTDD211 pKa = 3.44 DD212 pKa = 3.96 TTDD215 pKa = 3.49 DD216 pKa = 3.85 TTTDD220 pKa = 3.85 DD221 pKa = 4.52 DD222 pKa = 4.14 TDD224 pKa = 3.6 EE225 pKa = 4.62 TGPSITSSTEE235 pKa = 3.69 SSFLTTEE242 pKa = 3.76 QVILVVSTDD251 pKa = 3.18 EE252 pKa = 4.78 NATCRR257 pKa = 11.84 YY258 pKa = 7.88 NVKK261 pKa = 10.29 YY262 pKa = 10.84 DD263 pKa = 3.56 EE264 pKa = 5.97 DD265 pKa = 4.09 YY266 pKa = 11.86 GEE268 pKa = 4.19 MGGILSSSFGTGHH281 pKa = 6.95 HH282 pKa = 6.29 IVLGTLTEE290 pKa = 4.09 GDD292 pKa = 3.44 YY293 pKa = 11.01 TFYY296 pKa = 11.07 VRR298 pKa = 11.84 CKK300 pKa = 10.42 DD301 pKa = 3.34 EE302 pKa = 4.45 SGNISTAGTEE312 pKa = 3.88 VTFAVSADD320 pKa = 3.57 DD321 pKa = 3.01 TGAPVRR327 pKa = 11.84 SEE329 pKa = 4.55 LSPSSTVSSTSVTLSLSTDD348 pKa = 3.01 VAATCKK354 pKa = 9.76 YY355 pKa = 5.36 TTSDD359 pKa = 3.57 SGMDD363 pKa = 3.54 YY364 pKa = 11.61 NDD366 pKa = 3.33 MKK368 pKa = 11.2 KK369 pKa = 10.34 EE370 pKa = 3.74 FDD372 pKa = 3.25 ATGGISHH379 pKa = 6.45 SEE381 pKa = 4.27 SVSSLVVGEE390 pKa = 4.42 SYY392 pKa = 10.3 TYY394 pKa = 10.08 YY395 pKa = 10.52 VRR397 pKa = 11.84 CKK399 pKa = 10.54 SISTGVANTSSTEE412 pKa = 3.55 ISFTVDD418 pKa = 3.32 DD419 pKa = 4.58 TDD421 pKa = 3.69 STAPTITSTTSSTFAGTDD439 pKa = 3.48 EE440 pKa = 4.86 VILTVTTNEE449 pKa = 3.9 TAYY452 pKa = 10.48 CGYY455 pKa = 10.47 SDD457 pKa = 4.73 VYY459 pKa = 10.71 SIDD462 pKa = 3.42 MADD465 pKa = 3.67 YY466 pKa = 8.77 EE467 pKa = 4.79 ANPMVTSDD475 pKa = 3.67 GVTHH479 pKa = 6.84 KK480 pKa = 10.97 ASLGVLSTASYY491 pKa = 9.32 TYY493 pKa = 10.94 YY494 pKa = 10.0 IGCYY498 pKa = 9.71 DD499 pKa = 3.41 EE500 pKa = 5.17 SGNVTTFPAEE510 pKa = 3.65 ILFDD514 pKa = 3.71 VASLYY519 pKa = 10.99 KK520 pKa = 10.71 NSIFYY525 pKa = 10.95 AEE527 pKa = 4.19 NQVASSSPMKK537 pKa = 10.75 AGFTTALEE545 pKa = 4.39 SVGNLFVSTAIAQDD559 pKa = 3.6 TTDD562 pKa = 3.82 TTDD565 pKa = 2.87 STTDD569 pKa = 3.02 TTDD572 pKa = 3.88 ADD574 pKa = 4.2 TEE576 pKa = 4.19 DD577 pKa = 4.2 SNFLEE582 pKa = 4.75 EE583 pKa = 4.48 GSVDD587 pKa = 3.49 EE588 pKa = 5.47 GSGTGQFTSKK598 pKa = 11.13 LEE600 pKa = 4.01 DD601 pKa = 3.61 LKK603 pKa = 11.22 PGTFFYY609 pKa = 11.1 ARR611 pKa = 11.84 AYY613 pKa = 10.31 AVIGGTTYY621 pKa = 10.97 YY622 pKa = 10.9 GNQISFQTADD632 pKa = 3.32 SCFVATAAYY641 pKa = 10.02 GSLFHH646 pKa = 7.09 PAVQLLRR653 pKa = 11.84 DD654 pKa = 3.59 FRR656 pKa = 11.84 DD657 pKa = 3.25 RR658 pKa = 11.84 FMLDD662 pKa = 3.18 NPVSRR667 pKa = 11.84 SMVRR671 pKa = 11.84 LYY673 pKa = 10.95 YY674 pKa = 10.24 RR675 pKa = 11.84 YY676 pKa = 9.76 SPPIADD682 pKa = 4.02 VISSNTVLRR691 pKa = 11.84 PVTRR695 pKa = 11.84 TLLLPIVGSAWLTMHH710 pKa = 7.15 FGWLWLILPAATMALLSWFGMQRR733 pKa = 11.84 MQAARR738 pKa = 11.84 KK739 pKa = 9.57 EE740 pKa = 4.17 EE741 pKa = 4.0 LAA743 pKa = 4.54
Molecular weight: 79.13 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.708
IPC2_protein 3.859
IPC_protein 3.884
Toseland 3.668
ProMoST 4.024
Dawson 3.872
Bjellqvist 4.012
Wikipedia 3.783
Rodwell 3.706
Grimsley 3.567
Solomon 3.859
Lehninger 3.821
Nozaki 3.973
DTASelect 4.202
Thurlkill 3.706
EMBOSS 3.795
Sillero 3.999
Patrickios 1.303
IPC_peptide 3.859
IPC2_peptide 3.986
IPC2.peptide.svr19 3.887
Protein with the highest isoelectric point:
>tr|A0A3S3QQZ8|A0A3S3QQZ8_9DELT Uncharacterized protein OS=Candidatus Electrothrix aarhusiensis OX=1859131 GN=H206_01075 PE=4 SV=1
MM1 pKa = 7.69 SKK3 pKa = 9.01 RR4 pKa = 11.84 TFQPSNLKK12 pKa = 10.14 RR13 pKa = 11.84 KK14 pKa = 8.66 RR15 pKa = 11.84 SHH17 pKa = 5.99 GFRR20 pKa = 11.84 VRR22 pKa = 11.84 MKK24 pKa = 9.0 TASGQAIIKK33 pKa = 9.81 RR34 pKa = 11.84 RR35 pKa = 11.84 RR36 pKa = 11.84 AKK38 pKa = 9.88 GRR40 pKa = 11.84 KK41 pKa = 8.85 RR42 pKa = 11.84 LSAA45 pKa = 3.96
Molecular weight: 5.28 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.517
IPC2_protein 11.213
IPC_protein 12.793
Toseland 12.969
ProMoST 13.451
Dawson 12.969
Bjellqvist 12.954
Wikipedia 13.437
Rodwell 12.705
Grimsley 12.998
Solomon 13.451
Lehninger 13.364
Nozaki 12.969
DTASelect 12.954
Thurlkill 12.969
EMBOSS 13.466
Sillero 12.969
Patrickios 12.427
IPC_peptide 13.466
IPC2_peptide 12.442
IPC2.peptide.svr19 9.142
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
4071
0
4071
1082977
21
1712
266.0
29.76
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
7.926 ± 0.04
1.389 ± 0.019
5.455 ± 0.043
6.806 ± 0.042
4.358 ± 0.031
6.973 ± 0.042
2.146 ± 0.018
6.485 ± 0.036
5.837 ± 0.044
10.425 ± 0.055
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.496 ± 0.02
3.795 ± 0.032
4.277 ± 0.027
4.199 ± 0.031
5.424 ± 0.029
6.183 ± 0.034
5.215 ± 0.034
6.44 ± 0.033
1.14 ± 0.018
3.029 ± 0.026
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here