Streptomyces sp. BK340

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Actinobacteria; Actinomycetia; Streptomycetales; Streptomycetaceae; Streptomyces; unclassified Streptomyces

Average proteome isoelectric point is 6.48

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 9141 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A559URA3|A0A559URA3_9ACTN Uncharacterized protein OS=Streptomyces sp. BK340 OX=2572903 GN=FB157_11378 PE=4 SV=1
MM1 pKa = 6.61QQEE4 pKa = 4.4APGGEE9 pKa = 4.07ALEE12 pKa = 4.15VWIDD16 pKa = 3.41QDD18 pKa = 3.99LCTGDD23 pKa = 5.01GICAQYY29 pKa = 10.9APEE32 pKa = 4.23VFEE35 pKa = 5.9LDD37 pKa = 3.05IDD39 pKa = 3.65GLAYY43 pKa = 10.7VKK45 pKa = 10.83GADD48 pKa = 4.78DD49 pKa = 4.1EE50 pKa = 5.13LLLHH54 pKa = 6.99EE55 pKa = 5.27GATAPVPLPLLTDD68 pKa = 3.6VVDD71 pKa = 4.12SAKK74 pKa = 10.15EE75 pKa = 3.93CPGEE79 pKa = 4.38CIHH82 pKa = 6.4VRR84 pKa = 11.84RR85 pKa = 11.84VSDD88 pKa = 3.27KK89 pKa = 10.85VEE91 pKa = 4.24VYY93 pKa = 10.9GPDD96 pKa = 3.2ADD98 pKa = 3.69

Molecular weight: 10.5 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A559V4L7|A0A559V4L7_9ACTN Uncharacterized protein OS=Streptomyces sp. BK340 OX=2572903 GN=FB157_105209 PE=4 SV=1
MM1 pKa = 7.69SKK3 pKa = 9.0RR4 pKa = 11.84TFQPNNRR11 pKa = 11.84RR12 pKa = 11.84RR13 pKa = 11.84AKK15 pKa = 8.7THH17 pKa = 5.15GFRR20 pKa = 11.84LRR22 pKa = 11.84MRR24 pKa = 11.84TRR26 pKa = 11.84AGRR29 pKa = 11.84AILANRR35 pKa = 11.84RR36 pKa = 11.84AKK38 pKa = 10.46GRR40 pKa = 11.84ASLSAA45 pKa = 3.83

Molecular weight: 5.22 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

9141

0

9141

3005390

25

14364

328.8

35.26

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

13.526 ± 0.044

0.823 ± 0.008

5.942 ± 0.022

5.657 ± 0.028

2.701 ± 0.013

9.335 ± 0.023

2.425 ± 0.012

3.04 ± 0.019

2.127 ± 0.023

10.456 ± 0.035

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.682 ± 0.012

1.76 ± 0.014

6.119 ± 0.024

2.869 ± 0.019

8.182 ± 0.032

5.114 ± 0.024

6.177 ± 0.026

8.407 ± 0.025

1.557 ± 0.011

2.102 ± 0.014
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski