Amino acid dipepetide frequency for Streptomyces sp. BK340

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10^-3.

For more information see sequence space article on Wikipedia.

	Ala	Cys	Asp	Glu	Phe	Gly	His	Ile	Lys	Leu	Met	Asn	Pro	Gln	Arg	Ser	Thr	Val	Trp	Tyr	Xaa
Ala	21.262AlaAla: 21.262 ± 0.143	1.11AlaCys: 1.11 ± 0.022	8.274AlaAsp: 8.274 ± 0.06	8.892AlaGlu: 8.892 ± 0.078	3.486AlaPhe: 3.486 ± 0.041	12.872AlaGly: 12.872 ± 0.089	3.068AlaHis: 3.068 ± 0.035	3.225AlaIle: 3.225 ± 0.037	2.773AlaLys: 2.773 ± 0.04	14.698AlaLeu: 14.698 ± 0.106	2.396AlaMet: 2.396 ± 0.032	1.898AlaAsn: 1.898 ± 0.029	7.054AlaPro: 7.054 ± 0.071	3.948AlaGln: 3.948 ± 0.043	10.499AlaArg: 10.499 ± 0.088	5.864AlaSer: 5.864 ± 0.045	6.971AlaThr: 6.971 ± 0.059	12.294AlaVal: 12.294 ± 0.08	1.907AlaTrp: 1.907 ± 0.024	2.763AlaTyr: 2.763 ± 0.034	0.0AlaXaa: 0.0 ± 0.0
Cys	1.113CysAla: 1.113 ± 0.022	0.1CysCys: 0.1 ± 0.006	0.48CysAsp: 0.48 ± 0.013	0.412CysGlu: 0.412 ± 0.011	0.234CysPhe: 0.234 ± 0.009	0.972CysGly: 0.972 ± 0.02	0.205CysHis: 0.205 ± 0.009	0.173CysIle: 0.173 ± 0.007	0.12CysLys: 0.12 ± 0.007	0.816CysLeu: 0.816 ± 0.016	0.123CysMet: 0.123 ± 0.007	0.144CysAsn: 0.144 ± 0.007	0.522CysPro: 0.522 ± 0.017	0.182CysGln: 0.182 ± 0.007	0.656CysArg: 0.656 ± 0.016	0.475CysSer: 0.475 ± 0.014	0.541CysThr: 0.541 ± 0.015	0.681CysVal: 0.681 ± 0.017	0.126CysTrp: 0.126 ± 0.005	0.159CysTyr: 0.159 ± 0.008	0.0CysXaa: 0.0 ± 0.0
Asp	7.426AspAla: 7.426 ± 0.057	0.441AspCys: 0.441 ± 0.013	3.528AspAsp: 3.528 ± 0.036	3.804AspGlu: 3.804 ± 0.041	1.684AspPhe: 1.684 ± 0.023	6.255AspGly: 6.255 ± 0.053	1.484AspHis: 1.484 ± 0.024	1.822AspIle: 1.822 ± 0.025	1.16AspLys: 1.16 ± 0.029	6.286AspLeu: 6.286 ± 0.054	0.791AspMet: 0.791 ± 0.016	0.973AspAsn: 0.973 ± 0.021	4.455AspPro: 4.455 ± 0.043	1.574AspGln: 1.574 ± 0.027	5.005AspArg: 5.005 ± 0.044	2.615AspSer: 2.615 ± 0.029	3.297AspThr: 3.297 ± 0.037	4.668AspVal: 4.668 ± 0.037	1.057AspTrp: 1.057 ± 0.022	1.098AspTyr: 1.098 ± 0.019	0.0AspXaa: 0.0 ± 0.0
Glu	7.457GluAla: 7.457 ± 0.073	0.378GluCys: 0.378 ± 0.011	2.791GluAsp: 2.791 ± 0.033	3.568GluGlu: 3.568 ± 0.046	1.462GluPhe: 1.462 ± 0.023	4.227GluGly: 4.227 ± 0.037	1.579GluHis: 1.579 ± 0.026	2.178GluIle: 2.178 ± 0.031	1.345GluLys: 1.345 ± 0.026	6.754GluLeu: 6.754 ± 0.054	0.858GluMet: 0.858 ± 0.019	1.023GluAsn: 1.023 ± 0.019	3.399GluPro: 3.399 ± 0.041	2.363GluGln: 2.363 ± 0.034	5.486GluArg: 5.486 ± 0.056	2.568GluSer: 2.568 ± 0.033	2.871GluThr: 2.871 ± 0.035	4.344GluVal: 4.344 ± 0.042	0.808GluTrp: 0.808 ± 0.017	1.111GluTyr: 1.111 ± 0.02	0.0GluXaa: 0.0 ± 0.0
Phe	3.594PheAla: 3.594 ± 0.037	0.268PheCys: 0.268 ± 0.009	1.927PheAsp: 1.927 ± 0.028	1.444PheGlu: 1.444 ± 0.024	0.865PhePhe: 0.865 ± 0.018	2.971PheGly: 2.971 ± 0.034	0.646PheHis: 0.646 ± 0.014	0.632PheIle: 0.632 ± 0.017	0.507PheLys: 0.507 ± 0.015	2.602PheLeu: 2.602 ± 0.033	0.39PheMet: 0.39 ± 0.012	0.548PheAsn: 0.548 ± 0.014	1.401PhePro: 1.401 ± 0.024	0.692PheGln: 0.692 ± 0.015	1.881PheArg: 1.881 ± 0.028	1.434PheSer: 1.434 ± 0.026	2.005PheThr: 2.005 ± 0.03	2.221PheVal: 2.221 ± 0.031	0.413PheTrp: 0.413 ± 0.012	0.572PheTyr: 0.572 ± 0.013	0.0PheXaa: 0.0 ± 0.0
Gly	10.718GlyAla: 10.718 ± 0.074	0.855GlyCys: 0.855 ± 0.018	5.077GlyAsp: 5.077 ± 0.043	4.921GlyGlu: 4.921 ± 0.04	2.783GlyPhe: 2.783 ± 0.035	8.427GlyGly: 8.427 ± 0.076	2.511GlyHis: 2.511 ± 0.034	3.33GlyIle: 3.33 ± 0.037	2.396GlyLys: 2.396 ± 0.038	9.493GlyLeu: 9.493 ± 0.07	1.902GlyMet: 1.902 ± 0.027	1.775GlyAsn: 1.775 ± 0.027	5.184GlyPro: 5.184 ± 0.048	2.67GlyGln: 2.67 ± 0.04	8.026GlyArg: 8.026 ± 0.066	5.492GlySer: 5.492 ± 0.051	6.402GlyThr: 6.402 ± 0.054	7.422GlyVal: 7.422 ± 0.057	1.726GlyTrp: 1.726 ± 0.026	2.237GlyTyr: 2.237 ± 0.026	0.0GlyXaa: 0.0 ± 0.0
His	2.882HisAla: 2.882 ± 0.031	0.223HisCys: 0.223 ± 0.009	1.41HisAsp: 1.41 ± 0.022	1.296HisGlu: 1.296 ± 0.022	0.668HisPhe: 0.668 ± 0.016	2.578HisGly: 2.578 ± 0.032	0.763HisHis: 0.763 ± 0.02	0.722HisIle: 0.722 ± 0.016	0.391HisLys: 0.391 ± 0.012	2.513HisLeu: 2.513 ± 0.034	0.35HisMet: 0.35 ± 0.011	0.398HisAsn: 0.398 ± 0.013	1.922HisPro: 1.922 ± 0.03	0.669HisGln: 0.669 ± 0.015	2.256HisArg: 2.256 ± 0.029	1.088HisSer: 1.088 ± 0.02	1.457HisThr: 1.457 ± 0.026	1.777HisVal: 1.777 ± 0.026	0.391HisTrp: 0.391 ± 0.012	0.495HisTyr: 0.495 ± 0.013	0.0HisXaa: 0.0 ± 0.0
Ile	4.479IleAla: 4.479 ± 0.048	0.275IleCys: 0.275 ± 0.011	2.135IleAsp: 2.135 ± 0.03	1.891IleGlu: 1.891 ± 0.027	0.632IlePhe: 0.632 ± 0.016	3.403IleGly: 3.403 ± 0.042	0.611IleHis: 0.611 ± 0.014	0.784IleIle: 0.784 ± 0.019	0.698IleLys: 0.698 ± 0.019	2.339IleLeu: 2.339 ± 0.035	0.408IleMet: 0.408 ± 0.014	0.671IleAsn: 0.671 ± 0.015	1.734IlePro: 1.734 ± 0.023	0.736IleGln: 0.736 ± 0.016	2.303IleArg: 2.303 ± 0.027	1.636IleSer: 1.636 ± 0.023	2.185IleThr: 2.185 ± 0.028	2.614IleVal: 2.614 ± 0.038	0.367IleTrp: 0.367 ± 0.01	0.503IleTyr: 0.503 ± 0.012	0.0IleXaa: 0.0 ± 0.0
Lys	2.956LysAla: 2.956 ± 0.04	0.123LysCys: 0.123 ± 0.006	1.309LysAsp: 1.309 ± 0.029	1.129LysGlu: 1.129 ± 0.024	0.465LysPhe: 0.465 ± 0.015	1.812LysGly: 1.812 ± 0.035	0.44LysHis: 0.44 ± 0.013	0.839LysIle: 0.839 ± 0.021	0.855LysLys: 0.855 ± 0.027	1.957LysLeu: 1.957 ± 0.031	0.362LysMet: 0.362 ± 0.012	0.526LysAsn: 0.526 ± 0.015	1.328LysPro: 1.328 ± 0.025	0.719LysGln: 0.719 ± 0.019	1.358LysArg: 1.358 ± 0.023	1.177LysSer: 1.177 ± 0.021	1.273LysThr: 1.273 ± 0.027	1.868LysVal: 1.868 ± 0.032	0.29LysTrp: 0.29 ± 0.01	0.481LysTyr: 0.481 ± 0.016	0.0LysXaa: 0.0 ± 0.0
Leu	15.338LeuAla: 15.338 ± 0.114	0.877LeuCys: 0.877 ± 0.018	6.697LeuAsp: 6.697 ± 0.057	4.763LeuGlu: 4.763 ± 0.05	2.581LeuPhe: 2.581 ± 0.03	9.265LeuGly: 9.265 ± 0.066	2.456LeuHis: 2.456 ± 0.033	3.195LeuIle: 3.195 ± 0.04	2.059LeuLys: 2.059 ± 0.034	11.547LeuLeu: 11.547 ± 0.091	1.626LeuMet: 1.626 ± 0.028	1.678LeuAsn: 1.678 ± 0.024	6.669LeuPro: 6.669 ± 0.055	2.367LeuGln: 2.367 ± 0.027	8.913LeuArg: 8.913 ± 0.075	5.36LeuSer: 5.36 ± 0.045	7.007LeuThr: 7.007 ± 0.051	8.867LeuVal: 8.867 ± 0.062	1.392LeuTrp: 1.392 ± 0.024	1.9LeuTyr: 1.9 ± 0.023	0.0LeuXaa: 0.0 ± 0.0
Met	2.188MetAla: 2.188 ± 0.032	0.135MetCys: 0.135 ± 0.007	0.84MetAsp: 0.84 ± 0.017	0.72MetGlu: 0.72 ± 0.015	0.438MetPhe: 0.438 ± 0.011	1.254MetGly: 1.254 ± 0.024	0.387MetHis: 0.387 ± 0.013	0.609MetIle: 0.609 ± 0.016	0.398MetLys: 0.398 ± 0.011	1.617MetLeu: 1.617 ± 0.024	0.276MetMet: 0.276 ± 0.011	0.428MetAsn: 0.428 ± 0.013	1.088MetPro: 1.088 ± 0.02	0.459MetGln: 0.459 ± 0.015	1.376MetArg: 1.376 ± 0.025	1.341MetSer: 1.341 ± 0.02	1.532MetThr: 1.532 ± 0.025	1.208MetVal: 1.208 ± 0.019	0.208MetTrp: 0.208 ± 0.008	0.315MetTyr: 0.315 ± 0.011	0.0MetXaa: 0.0 ± 0.0
Asn	2.187AsnAla: 2.187 ± 0.028	0.174AsnCys: 0.174 ± 0.007	0.934AsnAsp: 0.934 ± 0.02	0.794AsnGlu: 0.794 ± 0.018	0.476AsnPhe: 0.476 ± 0.013	1.894AsnGly: 1.894 ± 0.031	0.411AsnHis: 0.411 ± 0.011	0.614AsnIle: 0.614 ± 0.015	0.409AsnLys: 0.409 ± 0.013	1.685AsnLeu: 1.685 ± 0.025	0.294AsnMet: 0.294 ± 0.011	0.431AsnAsn: 0.431 ± 0.013	1.367AsnPro: 1.367 ± 0.022	0.518AsnGln: 0.518 ± 0.013	1.271AsnArg: 1.271 ± 0.021	0.972AsnSer: 0.972 ± 0.017	1.133AsnThr: 1.133 ± 0.026	1.342AsnVal: 1.342 ± 0.023	0.296AsnTrp: 0.296 ± 0.01	0.397AsnTyr: 0.397 ± 0.012	0.0AsnXaa: 0.0 ± 0.0
Pro	8.816ProAla: 8.816 ± 0.078	0.385ProCys: 0.385 ± 0.011	4.583ProAsp: 4.583 ± 0.045	4.421ProGlu: 4.421 ± 0.046	1.508ProPhe: 1.508 ± 0.022	6.651ProGly: 6.651 ± 0.055	1.48ProHis: 1.48 ± 0.023	1.2ProIle: 1.2 ± 0.022	1.196ProLys: 1.196 ± 0.02	5.508ProLeu: 5.508 ± 0.054	0.98ProMet: 0.98 ± 0.019	0.894ProAsn: 0.894 ± 0.018	3.505ProPro: 3.505 ± 0.043	1.814ProGln: 1.814 ± 0.032	3.989ProArg: 3.989 ± 0.033	3.202ProSer: 3.202 ± 0.036	3.157ProThr: 3.157 ± 0.033	5.533ProVal: 5.533 ± 0.039	0.92ProTrp: 0.92 ± 0.02	1.447ProTyr: 1.447 ± 0.027	0.0ProXaa: 0.0 ± 0.0
Gln	3.909GlnAla: 3.909 ± 0.036	0.186GlnCys: 0.186 ± 0.008	1.517GlnAsp: 1.517 ± 0.026	1.534GlnGlu: 1.534 ± 0.028	0.677GlnPhe: 0.677 ± 0.015	2.38GlnGly: 2.38 ± 0.033	0.721GlnHis: 0.721 ± 0.015	1.09GlnIle: 1.09 ± 0.018	0.649GlnLys: 0.649 ± 0.017	3.179GlnLeu: 3.179 ± 0.034	0.499GlnMet: 0.499 ± 0.013	0.515GlnAsn: 0.515 ± 0.014	1.793GlnPro: 1.793 ± 0.037	1.304GlnGln: 1.304 ± 0.033	2.436GlnArg: 2.436 ± 0.026	1.303GlnSer: 1.303 ± 0.025	1.422GlnThr: 1.422 ± 0.025	2.458GlnVal: 2.458 ± 0.029	0.473GlnTrp: 0.473 ± 0.013	0.64GlnTyr: 0.64 ± 0.016	0.0GlnXaa: 0.0 ± 0.0
Arg	10.036ArgAla: 10.036 ± 0.077	0.652ArgCys: 0.652 ± 0.016	4.205ArgAsp: 4.205 ± 0.036	4.721ArgGlu: 4.721 ± 0.048	2.383ArgPhe: 2.383 ± 0.03	5.786ArgGly: 5.786 ± 0.045	2.277ArgHis: 2.277 ± 0.027	3.264ArgIle: 3.264 ± 0.038	1.62ArgLys: 1.62 ± 0.029	9.275ArgLeu: 9.275 ± 0.073	1.715ArgMet: 1.715 ± 0.027	1.351ArgAsn: 1.351 ± 0.02	5.183ArgPro: 5.183 ± 0.052	2.49ArgGln: 2.49 ± 0.032	7.979ArgArg: 7.979 ± 0.07	4.182ArgSer: 4.182 ± 0.037	5.601ArgThr: 5.601 ± 0.048	5.854ArgVal: 5.854 ± 0.051	1.394ArgTrp: 1.394 ± 0.022	1.855ArgTyr: 1.855 ± 0.022	0.0ArgXaa: 0.0 ± 0.0
Ser	6.858SerAla: 6.858 ± 0.051	0.444SerCys: 0.444 ± 0.013	2.727SerAsp: 2.727 ± 0.029	2.398SerGlu: 2.398 ± 0.03	1.534SerPhe: 1.534 ± 0.026	6.056SerGly: 6.056 ± 0.052	1.044SerHis: 1.044 ± 0.018	1.355SerIle: 1.355 ± 0.022	1.022SerLys: 1.022 ± 0.024	4.932SerLeu: 4.932 ± 0.043	1.077SerMet: 1.077 ± 0.021	0.9SerAsn: 0.9 ± 0.021	3.239SerPro: 3.239 ± 0.035	1.3SerGln: 1.3 ± 0.027	3.753SerArg: 3.753 ± 0.041	2.946SerSer: 2.946 ± 0.041	3.042SerThr: 3.042 ± 0.04	4.299SerVal: 4.299 ± 0.035	0.949SerTrp: 0.949 ± 0.019	1.261SerTyr: 1.261 ± 0.022	0.0SerXaa: 0.0 ± 0.0
Thr	9.07ThrAla: 9.07 ± 0.071	0.454ThrCys: 0.454 ± 0.013	3.715ThrAsp: 3.715 ± 0.037	3.306ThrGlu: 3.306 ± 0.036	1.542ThrPhe: 1.542 ± 0.025	6.795ThrGly: 6.795 ± 0.061	1.301ThrHis: 1.301 ± 0.018	1.623ThrIle: 1.623 ± 0.026	1.158ThrLys: 1.158 ± 0.022	5.827ThrLeu: 5.827 ± 0.053	0.885ThrMet: 0.885 ± 0.016	0.968ThrAsn: 0.968 ± 0.022	4.142ThrPro: 4.142 ± 0.043	1.418ThrGln: 1.418 ± 0.023	4.015ThrArg: 4.015 ± 0.04	3.21ThrSer: 3.21 ± 0.045	3.91ThrThr: 3.91 ± 0.057	6.133ThrVal: 6.133 ± 0.05	0.934ThrTrp: 0.934 ± 0.018	1.366ThrTyr: 1.366 ± 0.023	0.0ThrXaa: 0.0 ± 0.0
Val	10.463ValAla: 10.463 ± 0.071	0.8ValCys: 0.8 ± 0.017	4.822ValAsp: 4.822 ± 0.045	4.563ValGlu: 4.563 ± 0.046	2.422ValPhe: 2.422 ± 0.031	6.408ValGly: 6.408 ± 0.057	2.049ValHis: 2.049 ± 0.025	2.728ValIle: 2.728 ± 0.032	1.716ValLys: 1.716 ± 0.033	9.622ValLeu: 9.622 ± 0.075	1.347ValMet: 1.347 ± 0.024	1.649ValAsn: 1.649 ± 0.024	5.349ValPro: 5.349 ± 0.052	2.176ValGln: 2.176 ± 0.031	7.361ValArg: 7.361 ± 0.057	4.336ValSer: 4.336 ± 0.041	5.691ValThr: 5.691 ± 0.05	7.799ValVal: 7.799 ± 0.069	1.196ValTrp: 1.196 ± 0.02	1.568ValTyr: 1.568 ± 0.024	0.0ValXaa: 0.0 ± 0.0
Trp	1.71TrpAla: 1.71 ± 0.023	0.16TrpCys: 0.16 ± 0.007	0.852TrpAsp: 0.852 ± 0.02	0.738TrpGlu: 0.738 ± 0.018	0.53TrpPhe: 0.53 ± 0.015	1.061TrpGly: 1.061 ± 0.021	0.405TrpHis: 0.405 ± 0.012	0.551TrpIle: 0.551 ± 0.013	0.395TrpLys: 0.395 ± 0.013	1.837TrpLeu: 1.837 ± 0.029	0.279TrpMet: 0.279 ± 0.009	0.419TrpAsn: 0.419 ± 0.013	0.792TrpPro: 0.792 ± 0.017	0.667TrpGln: 0.667 ± 0.016	1.4TrpArg: 1.4 ± 0.024	0.968TrpSer: 0.968 ± 0.017	1.057TrpThr: 1.057 ± 0.018	1.004TrpVal: 1.004 ± 0.019	0.358TrpTrp: 0.358 ± 0.012	0.389TrpTyr: 0.389 ± 0.013	0.0TrpXaa: 0.0 ± 0.0
Tyr	2.793TyrAla: 2.793 ± 0.026	0.195TyrCys: 0.195 ± 0.009	1.6TyrAsp: 1.6 ± 0.023	1.255TyrGlu: 1.255 ± 0.023	0.642TyrPhe: 0.642 ± 0.017	2.278TyrGly: 2.278 ± 0.03	0.411TyrHis: 0.411 ± 0.012	0.469TyrIle: 0.469 ± 0.013	0.405TyrLys: 0.405 ± 0.014	2.072TyrLeu: 2.072 ± 0.027	0.258TyrMet: 0.258 ± 0.009	0.412TyrAsn: 0.412 ± 0.014	1.062TyrPro: 1.062 ± 0.02	0.619TyrGln: 0.619 ± 0.014	1.861TyrArg: 1.861 ± 0.028	0.967TyrSer: 0.967 ± 0.02	1.215TyrThr: 1.215 ± 0.021	1.68TyrVal: 1.68 ± 0.025	0.368TyrTrp: 0.368 ± 0.011	0.458TyrTyr: 0.458 ± 0.013	0.0TyrXaa: 0.0 ± 0.0
Xaa	0.0XaaAla: 0.0 ± 0.0	0.0XaaCys: 0.0 ± 0.0	0.0XaaAsp: 0.0 ± 0.0	0.0XaaGlu: 0.0 ± 0.0	0.0XaaPhe: 0.0 ± 0.0	0.0XaaGly: 0.0 ± 0.0	0.0XaaHis: 0.0 ± 0.0	0.0XaaIle: 0.0 ± 0.0	0.0XaaLys: 0.0 ± 0.0	0.0XaaLeu: 0.0 ± 0.0	0.0XaaMet: 0.0 ± 0.0	0.0XaaAsn: 0.0 ± 0.0	0.0XaaPro: 0.0 ± 0.0	0.0XaaGln: 0.0 ± 0.0	0.0XaaArg: 0.0 ± 0.0	0.0XaaSer: 0.0 ± 0.0	0.0XaaThr: 0.0 ± 0.0	0.0XaaVal: 0.0 ± 0.0	0.0XaaTrp: 0.0 ± 0.0	0.0XaaTyr: 0.0 ± 0.0	0.0XaaXaa: 0.0 ± 0.0

Statistics based on 9141 proteins (3005390 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944

Contact: Lukasz P. Kozlowski

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