Lactococcus lactis subsp. cremoris TIFN6

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Firmicutes; Bacilli; Lactobacillales; Streptococcaceae; Lactococcus; Lactococcus cremoris; Lactococcus cremoris subsp. cremoris

Average proteome isoelectric point is 6.63

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2310 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|T0SCT1|T0SCT1_LACLC dUTP diphosphatase OS=Lactococcus lactis subsp. cremoris TIFN6 OX=1234876 GN=dut PE=4 SV=1
MM1 pKa = 7.15THH3 pKa = 5.34THH5 pKa = 5.82DD6 pKa = 4.73HH7 pKa = 5.77EE8 pKa = 6.31HH9 pKa = 6.75DD10 pKa = 4.22HH11 pKa = 5.96NHH13 pKa = 5.56EE14 pKa = 4.18PDD16 pKa = 3.97YY17 pKa = 10.77ITLVDD22 pKa = 3.78EE23 pKa = 5.1NGNEE27 pKa = 4.21SLFQILITIDD37 pKa = 3.34GQEE40 pKa = 4.18EE41 pKa = 4.04FGKK44 pKa = 10.43NYY46 pKa = 10.08VVLQPTEE53 pKa = 4.16FEE55 pKa = 4.04EE56 pKa = 5.26DD57 pKa = 3.4EE58 pKa = 4.15QGLIDD63 pKa = 3.63VLAYY67 pKa = 10.87SFTEE71 pKa = 4.05NADD74 pKa = 3.34GTEE77 pKa = 4.48GDD79 pKa = 4.3LQPIPEE85 pKa = 4.52DD86 pKa = 5.82AEE88 pKa = 5.1DD89 pKa = 3.67EE90 pKa = 3.88WDD92 pKa = 3.68MIEE95 pKa = 4.26EE96 pKa = 4.3VFNSFMDD103 pKa = 3.8EE104 pKa = 4.16QEE106 pKa = 4.15DD107 pKa = 3.66

Molecular weight: 12.43 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|T0TKB9|T0TKB9_LACLC Signal recognition particle protein OS=Lactococcus lactis subsp. cremoris TIFN6 OX=1234876 GN=ffh PE=3 SV=1
MM1 pKa = 7.28KK2 pKa = 9.42RR3 pKa = 11.84TYY5 pKa = 10.06QPHH8 pKa = 5.63KK9 pKa = 9.98KK10 pKa = 9.63SRR12 pKa = 11.84KK13 pKa = 4.36TTHH16 pKa = 6.07GFRR19 pKa = 11.84SRR21 pKa = 11.84MATKK25 pKa = 10.11NGRR28 pKa = 11.84RR29 pKa = 11.84VLAARR34 pKa = 11.84RR35 pKa = 11.84RR36 pKa = 11.84KK37 pKa = 9.65GRR39 pKa = 11.84ASLTVV44 pKa = 3.04

Molecular weight: 5.18 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2310

0

2310

515914

28

1635

223.3

25.06

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.3 ± 0.064

0.502 ± 0.014

5.345 ± 0.048

7.044 ± 0.074

4.7 ± 0.042

6.532 ± 0.06

1.762 ± 0.023

7.517 ± 0.053

7.506 ± 0.061

9.755 ± 0.068

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.596 ± 0.027

5.199 ± 0.046

3.254 ± 0.032

3.777 ± 0.031

3.748 ± 0.042

6.466 ± 0.054

5.776 ± 0.062

6.606 ± 0.049

0.986 ± 0.02

3.628 ± 0.041
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski