Amino acid dipepetide frequency for Lactococcus lactis subsp. cremoris TIFN6

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
5.487AlaAla: 5.487 ± 0.135
0.386AlaCys: 0.386 ± 0.028
4.185AlaAsp: 4.185 ± 0.1
4.912AlaGlu: 4.912 ± 0.111
3.33AlaPhe: 3.33 ± 0.072
5.138AlaGly: 5.138 ± 0.109
1.221AlaHis: 1.221 ± 0.054
5.613AlaIle: 5.613 ± 0.124
5.695AlaLys: 5.695 ± 0.11
7.302AlaLeu: 7.302 ± 0.126
1.797AlaMet: 1.797 ± 0.057
3.353AlaAsn: 3.353 ± 0.091
2.142AlaPro: 2.142 ± 0.082
3.115AlaGln: 3.115 ± 0.081
2.63AlaArg: 2.63 ± 0.083
4.299AlaSer: 4.299 ± 0.102
4.297AlaThr: 4.297 ± 0.111
4.941AlaVal: 4.941 ± 0.113
0.707AlaTrp: 0.707 ± 0.042
2.446AlaTyr: 2.446 ± 0.059
0.0AlaXaa: 0.0 ± 0.0
Cys
0.326CysAla: 0.326 ± 0.026
0.064CysCys: 0.064 ± 0.012
0.262CysAsp: 0.262 ± 0.019
0.318CysGlu: 0.318 ± 0.023
0.246CysPhe: 0.246 ± 0.021
0.43CysGly: 0.43 ± 0.031
0.178CysHis: 0.178 ± 0.018
0.308CysIle: 0.308 ± 0.024
0.333CysLys: 0.333 ± 0.026
0.545CysLeu: 0.545 ± 0.033
0.091CysMet: 0.091 ± 0.013
0.225CysAsn: 0.225 ± 0.019
0.204CysPro: 0.204 ± 0.021
0.223CysGln: 0.223 ± 0.021
0.153CysArg: 0.153 ± 0.019
0.308CysSer: 0.308 ± 0.026
0.236CysThr: 0.236 ± 0.023
0.275CysVal: 0.275 ± 0.023
0.048CysTrp: 0.048 ± 0.011
0.242CysTyr: 0.242 ± 0.021
0.0CysXaa: 0.0 ± 0.0
Asp
3.132AspAla: 3.132 ± 0.083
0.275AspCys: 0.275 ± 0.022
2.741AspAsp: 2.741 ± 0.077
4.72AspGlu: 4.72 ± 0.128
3.19AspPhe: 3.19 ± 0.087
3.625AspGly: 3.625 ± 0.097
0.835AspHis: 0.835 ± 0.043
3.731AspIle: 3.731 ± 0.088
4.448AspLys: 4.448 ± 0.103
5.342AspLeu: 5.342 ± 0.103
1.361AspMet: 1.361 ± 0.051
2.545AspAsn: 2.545 ± 0.089
1.504AspPro: 1.504 ± 0.054
1.558AspGln: 1.558 ± 0.064
1.874AspArg: 1.874 ± 0.071
3.324AspSer: 3.324 ± 0.094
2.574AspThr: 2.574 ± 0.078
3.32AspVal: 3.32 ± 0.081
0.75AspTrp: 0.75 ± 0.038
2.597AspTyr: 2.597 ± 0.073
0.0AspXaa: 0.0 ± 0.0
Glu
4.991GluAla: 4.991 ± 0.107
0.281GluCys: 0.281 ± 0.025
3.053GluAsp: 3.053 ± 0.074
5.569GluGlu: 5.569 ± 0.119
2.832GluPhe: 2.832 ± 0.081
3.392GluGly: 3.392 ± 0.086
1.241GluHis: 1.241 ± 0.05
6.148GluIle: 6.148 ± 0.128
6.309GluLys: 6.309 ± 0.127
7.474GluLeu: 7.474 ± 0.141
2.024GluMet: 2.024 ± 0.068
4.753GluAsn: 4.753 ± 0.109
1.679GluPro: 1.679 ± 0.062
2.948GluGln: 2.948 ± 0.088
2.925GluArg: 2.925 ± 0.085
3.499GluSer: 3.499 ± 0.09
3.817GluThr: 3.817 ± 0.089
4.861GluVal: 4.861 ± 0.11
0.562GluTrp: 0.562 ± 0.03
2.08GluTyr: 2.08 ± 0.069
0.002GluXaa: 0.002 ± 0.002
Phe
3.359PheAla: 3.359 ± 0.073
0.275PheCys: 0.275 ± 0.024
3.051PheAsp: 3.051 ± 0.092
3.332PheGlu: 3.332 ± 0.094
2.084PhePhe: 2.084 ± 0.079
3.481PheGly: 3.481 ± 0.098
0.742PheHis: 0.742 ± 0.038
3.382PheIle: 3.382 ± 0.096
2.997PheLys: 2.997 ± 0.079
4.301PheLeu: 4.301 ± 0.12
1.157PheMet: 1.157 ± 0.05
2.227PheAsn: 2.227 ± 0.069
1.746PhePro: 1.746 ± 0.065
1.564PheGln: 1.564 ± 0.065
1.494PheArg: 1.494 ± 0.053
3.472PheSer: 3.472 ± 0.092
2.667PheThr: 2.667 ± 0.069
3.216PheVal: 3.216 ± 0.081
0.531PheTrp: 0.531 ± 0.037
1.923PheTyr: 1.923 ± 0.073
0.0PheXaa: 0.0 ± 0.0
Gly
4.824GlyAla: 4.824 ± 0.1
0.335GlyCys: 0.335 ± 0.029
3.126GlyAsp: 3.126 ± 0.087
3.861GlyGlu: 3.861 ± 0.094
3.441GlyPhe: 3.441 ± 0.091
4.383GlyGly: 4.383 ± 0.125
1.308GlyHis: 1.308 ± 0.055
5.27GlyIle: 5.27 ± 0.109
4.824GlyLys: 4.824 ± 0.114
6.321GlyLeu: 6.321 ± 0.129
1.847GlyMet: 1.847 ± 0.059
3.101GlyAsn: 3.101 ± 0.09
1.341GlyPro: 1.341 ± 0.05
2.822GlyGln: 2.822 ± 0.076
2.464GlyArg: 2.464 ± 0.074
3.917GlySer: 3.917 ± 0.094
4.078GlyThr: 4.078 ± 0.099
4.479GlyVal: 4.479 ± 0.101
0.713GlyTrp: 0.713 ± 0.038
2.863GlyTyr: 2.863 ± 0.076
0.0GlyXaa: 0.0 ± 0.0
His
1.116HisAla: 1.116 ± 0.046
0.128HisCys: 0.128 ± 0.017
1.01HisAsp: 1.01 ± 0.043
1.221HisGlu: 1.221 ± 0.047
1.124HisPhe: 1.124 ± 0.044
1.208HisGly: 1.208 ± 0.049
0.432HisHis: 0.432 ± 0.027
1.149HisIle: 1.149 ± 0.048
0.956HisLys: 0.956 ± 0.046
1.859HisLeu: 1.859 ± 0.061
0.359HisMet: 0.359 ± 0.028
0.735HisAsn: 0.735 ± 0.038
0.781HisPro: 0.781 ± 0.038
0.855HisGln: 0.855 ± 0.041
0.75HisArg: 0.75 ± 0.04
1.039HisSer: 1.039 ± 0.041
0.832HisThr: 0.832 ± 0.038
1.029HisVal: 1.029 ± 0.044
0.186HisTrp: 0.186 ± 0.017
0.853HisTyr: 0.853 ± 0.042
0.0HisXaa: 0.0 ± 0.0
Ile
5.865IleAla: 5.865 ± 0.127
0.531IleCys: 0.531 ± 0.036
4.514IleAsp: 4.514 ± 0.091
5.121IleGlu: 5.121 ± 0.111
3.613IlePhe: 3.613 ± 0.099
5.084IleGly: 5.084 ± 0.135
1.266IleHis: 1.266 ± 0.055
5.825IleIle: 5.825 ± 0.143
5.14IleLys: 5.14 ± 0.11
7.133IleLeu: 7.133 ± 0.155
1.708IleMet: 1.708 ± 0.059
3.77IleAsn: 3.77 ± 0.092
2.828IlePro: 2.828 ± 0.075
2.444IleGln: 2.444 ± 0.075
2.58IleArg: 2.58 ± 0.076
5.425IleSer: 5.425 ± 0.102
4.113IleThr: 4.113 ± 0.086
4.966IleVal: 4.966 ± 0.126
0.737IleTrp: 0.737 ± 0.043
2.506IleTyr: 2.506 ± 0.073
0.0IleXaa: 0.0 ± 0.0
Lys
5.745LysAla: 5.745 ± 0.112
0.252LysCys: 0.252 ± 0.024
3.749LysAsp: 3.749 ± 0.092
6.133LysGlu: 6.133 ± 0.135
2.857LysPhe: 2.857 ± 0.078
3.975LysGly: 3.975 ± 0.086
1.229LysHis: 1.229 ± 0.052
6.079LysIle: 6.079 ± 0.114
6.191LysLys: 6.191 ± 0.116
6.35LysLeu: 6.35 ± 0.128
2.671LysMet: 2.671 ± 0.073
4.739LysAsn: 4.739 ± 0.099
1.944LysPro: 1.944 ± 0.068
2.69LysGln: 2.69 ± 0.071
3.169LysArg: 3.169 ± 0.09
4.487LysSer: 4.487 ± 0.093
4.253LysThr: 4.253 ± 0.104
5.22LysVal: 5.22 ± 0.093
0.717LysTrp: 0.717 ± 0.039
2.605LysTyr: 2.605 ± 0.076
0.0LysXaa: 0.0 ± 0.0
Leu
7.769LeuAla: 7.769 ± 0.131
0.415LeuCys: 0.415 ± 0.03
5.204LeuAsp: 5.204 ± 0.11
7.011LeuGlu: 7.011 ± 0.14
4.284LeuPhe: 4.284 ± 0.118
6.427LeuGly: 6.427 ± 0.123
1.591LeuHis: 1.591 ± 0.066
7.346LeuIle: 7.346 ± 0.154
7.143LeuLys: 7.143 ± 0.117
9.672LeuLeu: 9.672 ± 0.199
2.469LeuMet: 2.469 ± 0.08
4.565LeuAsn: 4.565 ± 0.103
3.694LeuPro: 3.694 ± 0.081
2.981LeuGln: 2.981 ± 0.09
3.522LeuArg: 3.522 ± 0.086
7.352LeuSer: 7.352 ± 0.134
6.205LeuThr: 6.205 ± 0.111
6.168LeuVal: 6.168 ± 0.113
0.799LeuTrp: 0.799 ± 0.044
2.935LeuTyr: 2.935 ± 0.081
0.0LeuXaa: 0.0 ± 0.0
Met
2.146MetAla: 2.146 ± 0.065
0.105MetCys: 0.105 ± 0.013
1.273MetAsp: 1.273 ± 0.049
1.498MetGlu: 1.498 ± 0.045
0.983MetPhe: 0.983 ± 0.045
1.653MetGly: 1.653 ± 0.058
0.403MetHis: 0.403 ± 0.032
2.121MetIle: 2.121 ± 0.07
2.215MetLys: 2.215 ± 0.064
2.126MetLeu: 2.126 ± 0.067
0.791MetMet: 0.791 ± 0.046
1.628MetAsn: 1.628 ± 0.051
0.93MetPro: 0.93 ± 0.047
0.837MetGln: 0.837 ± 0.039
1.018MetArg: 1.018 ± 0.047
1.673MetSer: 1.673 ± 0.058
2.291MetThr: 2.291 ± 0.067
1.516MetVal: 1.516 ± 0.056
0.163MetTrp: 0.163 ± 0.019
0.589MetTyr: 0.589 ± 0.032
0.0MetXaa: 0.0 ± 0.0
Asn
3.375AsnAla: 3.375 ± 0.088
0.295AsnCys: 0.295 ± 0.024
2.727AsnAsp: 2.727 ± 0.07
3.061AsnGlu: 3.061 ± 0.069
2.894AsnPhe: 2.894 ± 0.09
3.731AsnGly: 3.731 ± 0.09
1.109AsnHis: 1.109 ± 0.044
3.725AsnIle: 3.725 ± 0.096
3.565AsnLys: 3.565 ± 0.09
5.311AsnLeu: 5.311 ± 0.111
1.334AsnMet: 1.334 ± 0.048
2.597AsnAsn: 2.597 ± 0.087
2.409AsnPro: 2.409 ± 0.065
2.407AsnGln: 2.407 ± 0.073
1.919AsnArg: 1.919 ± 0.072
3.268AsnSer: 3.268 ± 0.101
2.46AsnThr: 2.46 ± 0.08
3.14AsnVal: 3.14 ± 0.076
0.597AsnTrp: 0.597 ± 0.035
2.07AsnTyr: 2.07 ± 0.071
0.0AsnXaa: 0.0 ± 0.0
Pro
2.409ProAla: 2.409 ± 0.065
0.124ProCys: 0.124 ± 0.017
1.855ProAsp: 1.855 ± 0.06
2.83ProGlu: 2.83 ± 0.071
1.576ProPhe: 1.576 ± 0.054
1.628ProGly: 1.628 ± 0.064
0.613ProHis: 0.613 ± 0.034
2.361ProIle: 2.361 ± 0.073
2.529ProLys: 2.529 ± 0.071
3.037ProLeu: 3.037 ± 0.082
0.733ProMet: 0.733 ± 0.036
1.932ProAsn: 1.932 ± 0.068
0.564ProPro: 0.564 ± 0.034
1.285ProGln: 1.285 ± 0.052
1.012ProArg: 1.012 ± 0.043
1.958ProSer: 1.958 ± 0.062
2.07ProThr: 2.07 ± 0.071
2.407ProVal: 2.407 ± 0.072
0.302ProTrp: 0.302 ± 0.024
1.31ProTyr: 1.31 ± 0.054
0.0ProXaa: 0.0 ± 0.0
Gln
3.055GlnAla: 3.055 ± 0.081
0.12GlnCys: 0.12 ± 0.014
1.531GlnAsp: 1.531 ± 0.059
3.002GlnGlu: 3.002 ± 0.088
1.659GlnPhe: 1.659 ± 0.059
2.152GlnGly: 2.152 ± 0.067
0.593GlnHis: 0.593 ± 0.03
2.878GlnIle: 2.878 ± 0.089
3.287GlnLys: 3.287 ± 0.081
3.747GlnLeu: 3.747 ± 0.089
1.113GlnMet: 1.113 ± 0.042
2.117GlnAsn: 2.117 ± 0.066
1.153GlnPro: 1.153 ± 0.043
1.275GlnGln: 1.275 ± 0.063
1.341GlnArg: 1.341 ± 0.05
2.305GlnSer: 2.305 ± 0.076
2.281GlnThr: 2.281 ± 0.07
2.572GlnVal: 2.572 ± 0.068
0.331GlnTrp: 0.331 ± 0.026
1.26GlnTyr: 1.26 ± 0.051
0.0GlnXaa: 0.0 ± 0.0
Arg
2.514ArgAla: 2.514 ± 0.067
0.283ArgCys: 0.283 ± 0.024
1.863ArgAsp: 1.863 ± 0.064
2.799ArgGlu: 2.799 ± 0.088
1.71ArgPhe: 1.71 ± 0.051
2.194ArgGly: 2.194 ± 0.076
0.686ArgHis: 0.686 ± 0.035
2.578ArgIle: 2.578 ± 0.074
3.287ArgLys: 3.287 ± 0.077
3.646ArgLeu: 3.646 ± 0.086
1.076ArgMet: 1.076 ± 0.049
1.938ArgAsn: 1.938 ± 0.063
1.21ArgPro: 1.21 ± 0.048
1.549ArgGln: 1.549 ± 0.062
2.039ArgArg: 2.039 ± 0.066
1.948ArgSer: 1.948 ± 0.06
1.936ArgThr: 1.936 ± 0.063
2.45ArgVal: 2.45 ± 0.086
0.364ArgTrp: 0.364 ± 0.031
1.413ArgTyr: 1.413 ± 0.053
0.0ArgXaa: 0.0 ± 0.0
Ser
4.412SerAla: 4.412 ± 0.099
0.304SerCys: 0.304 ± 0.025
3.543SerAsp: 3.543 ± 0.106
4.348SerGlu: 4.348 ± 0.09
3.088SerPhe: 3.088 ± 0.082
4.993SerGly: 4.993 ± 0.124
1.113SerHis: 1.113 ± 0.052
4.404SerIle: 4.404 ± 0.112
4.724SerLys: 4.724 ± 0.11
6.472SerLeu: 6.472 ± 0.114
1.527SerMet: 1.527 ± 0.058
3.126SerAsn: 3.126 ± 0.096
2.117SerPro: 2.117 ± 0.06
2.749SerGln: 2.749 ± 0.089
2.322SerArg: 2.322 ± 0.067
4.652SerSer: 4.652 ± 0.153
3.673SerThr: 3.673 ± 0.105
4.163SerVal: 4.163 ± 0.093
0.725SerTrp: 0.725 ± 0.04
2.208SerTyr: 2.208 ± 0.072
0.0SerXaa: 0.0 ± 0.0
Thr
4.303ThrAla: 4.303 ± 0.099
0.219ThrCys: 0.219 ± 0.022
3.594ThrAsp: 3.594 ± 0.091
3.57ThrGlu: 3.57 ± 0.108
2.611ThrPhe: 2.611 ± 0.066
4.388ThrGly: 4.388 ± 0.136
0.946ThrHis: 0.946 ± 0.04
4.293ThrIle: 4.293 ± 0.094
3.805ThrLys: 3.805 ± 0.09
5.497ThrLeu: 5.497 ± 0.102
1.204ThrMet: 1.204 ± 0.049
2.95ThrAsn: 2.95 ± 0.093
2.355ThrPro: 2.355 ± 0.084
1.95ThrGln: 1.95 ± 0.063
1.843ThrArg: 1.843 ± 0.059
3.867ThrSer: 3.867 ± 0.101
3.528ThrThr: 3.528 ± 0.115
4.123ThrVal: 4.123 ± 0.102
0.514ThrTrp: 0.514 ± 0.032
2.204ThrTyr: 2.204 ± 0.071
0.0ThrXaa: 0.0 ± 0.0
Val
5.402ValAla: 5.402 ± 0.113
0.376ValCys: 0.376 ± 0.027
3.499ValAsp: 3.499 ± 0.101
4.34ValGlu: 4.34 ± 0.115
3.041ValPhe: 3.041 ± 0.078
4.361ValGly: 4.361 ± 0.1
1.054ValHis: 1.054 ± 0.044
5.086ValIle: 5.086 ± 0.104
4.724ValLys: 4.724 ± 0.105
6.303ValLeu: 6.303 ± 0.121
1.717ValMet: 1.717 ± 0.057
3.167ValAsn: 3.167 ± 0.067
2.429ValPro: 2.429 ± 0.068
2.091ValGln: 2.091 ± 0.065
2.38ValArg: 2.38 ± 0.077
4.671ValSer: 4.671 ± 0.102
4.078ValThr: 4.078 ± 0.097
4.46ValVal: 4.46 ± 0.11
0.574ValTrp: 0.574 ± 0.035
2.303ValTyr: 2.303 ± 0.075
0.002ValXaa: 0.002 ± 0.002
Trp
0.591TrpAla: 0.591 ± 0.035
0.06TrpCys: 0.06 ± 0.011
0.512TrpAsp: 0.512 ± 0.032
0.587TrpGlu: 0.587 ± 0.035
0.488TrpPhe: 0.488 ± 0.033
0.715TrpGly: 0.715 ± 0.046
0.238TrpHis: 0.238 ± 0.022
0.719TrpIle: 0.719 ± 0.037
0.682TrpLys: 0.682 ± 0.037
1.186TrpLeu: 1.186 ± 0.056
0.256TrpMet: 0.256 ± 0.022
0.566TrpAsn: 0.566 ± 0.036
0.171TrpPro: 0.171 ± 0.016
0.415TrpGln: 0.415 ± 0.026
0.38TrpArg: 0.38 ± 0.028
0.64TrpSer: 0.64 ± 0.044
0.539TrpThr: 0.539 ± 0.034
0.614TrpVal: 0.614 ± 0.04
0.155TrpTrp: 0.155 ± 0.018
0.349TrpTyr: 0.349 ± 0.026
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.175TyrAla: 2.175 ± 0.073
0.188TyrCys: 0.188 ± 0.021
2.157TyrAsp: 2.157 ± 0.067
2.206TyrGlu: 2.206 ± 0.075
1.952TyrPhe: 1.952 ± 0.066
2.361TyrGly: 2.361 ± 0.072
0.82TyrHis: 0.82 ± 0.039
2.152TyrIle: 2.152 ± 0.07
2.2TyrLys: 2.2 ± 0.064
3.927TyrLeu: 3.927 ± 0.085
0.725TyrMet: 0.725 ± 0.039
1.958TyrAsn: 1.958 ± 0.072
1.335TyrPro: 1.335 ± 0.049
2.012TyrGln: 2.012 ± 0.069
1.667TyrArg: 1.667 ± 0.054
2.561TyrSer: 2.561 ± 0.08
1.836TyrThr: 1.836 ± 0.075
2.138TyrVal: 2.138 ± 0.066
0.388TyrTrp: 0.388 ± 0.03
1.524TyrTyr: 1.524 ± 0.063
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.002XaaHis: 0.002 ± 0.002
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.002XaaGln: 0.002 ± 0.002
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 2310 proteins (515914 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski